| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,379,626 | T→C | 7.3% | H189R (CAT→CGT) | tnpS ← | cointegrate resolution protein S |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,379,626 | 0 | T | C | 7.3% | 157.5 / 2.4 | 55 | H189R (CAT→CGT) | tnpS | cointegrate resolution protein S |
| Reads supporting (aligned to +/- strand): ref base T (22/29); new base C (2/2); total (24/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.52e-01 | |||||||||||
TGATCAGGCTGCCGGGATGCAAGGCCTCGTCGCTCAGGTGCCCCCAGCGATCCAGGCG‑ACAGAACACTGCACCACGGGCAATGCCGGCGGCACTGATCCAGTCGAGATAAGCCTGCACCGGGCAGAGGCACTTCAAGGCTGGTACGTAATGAGTCGTGCCGAGGTTGTCCCTGTCACCCTTACTTTGTGGCAGGAACAGGCTCATGCCCGCACCGGCTTCGGCCTGGATATGTTCGACCTGCAGCCGACAGAGTTCGTCACTACGAAAACCCCGCCAGAAGCCGATCAGCAGCAACG > NC_002947/3379477‑3379773 | tGATCAGGCTGCCGGGATGCAAGGCCTCGTCGCTCAGGTGCCCCCAGCGATCCAGGCG‑ACAGAACACTGCACCACGGGCAATGCCGGCGGCACTGATCCAGTCGAGATAAGCCTGCACCGGGCAGAGGCACTTCAAGGCTGGTACGTAAt > 1:462579/1‑150 (MQ=255) cAGGCTGCCGTGATTCAAGTCCTCGTCGCTCAGGTGCCCCCAGCGATCCAGACGTGCCCAACAATTC‑GTACGGTTAATGCCGGCGGTACTGTGCCAGCCGAGATAAGCCTGCACCGGGCAGAGGCACTTCAAGGCTGGTACGTAATGAGt < 1:252372/150‑1 (MQ=255) gCTGCCGGGATGCAAGGCCTCGTCGCTCAGGTGCCCCCAGCGATCCAGGCG‑ACAGAACACTGCACCACGGGCAATGCCGGCGGCACTGATCCAGTCGAGATAAGCCTGCACCGGGCAGAGGCGCATCAAGGCTGGTACGTTATGAggcat > 2:906584/1‑146 (MQ=255) cGGGATGCAAGGCCTCGTCGCTCAGGTGCCCCCAGCGATCCAGGCG‑ACAGAACACTGCACCACGGGCAATGCCGGCGGCACTGATCCAGTCGAGATAAGCCTGCACCGGGCAGAGGCACTTCAAGGCTGGTACGTAATGAGTCGTGCCGa < 2:278734/150‑1 (MQ=255) cGGGATGCAAGGCCTCGTCGCTCAGGTGCCCCCAGCGATCCAGGCG‑ACAGAACACTGCACCACGGGCAATGCCGGCGGCACTGATCCAGTCGAGATAAGCCTGCACCGGGCAGAGGCACTTCAAGGCTGGTACGTAACGAGTCGTGCCGa > 2:579829/1‑150 (MQ=255) gtcgCTCAGGTGCCCCCAGCGATCCAGGCG‑ACAGAACACTGCACCACGGGCAATGCCGGCGGCACTGATCCAGTCGAGATAAGCCTGCACCGGGCAGAGGCACTTCAAGGCTGGTACGTAATGAGTCGTGCCGAGGTTGTCCCTGTCAcc < 1:906584/150‑1 (MQ=255) tCAGGTGCCCCCAGCGATCCAGGCG‑ACAGAACACTGCACCACGGGCAATGCCGGCGGCACTGATCCAGTCGAGATAAGCCTGCACCGGGCAGAGGCACTTCAAGGCTGGTACGTAATGAGTCGTGCCGAGGTTGTCCCTGTCACCCTTAc > 1:133422/1‑150 (MQ=255) tCAGGTGCCCCCAGCGATCCAGGCG‑ACAGAACACTGCACCACGGGCAATGCCGGCGGCACTGATCCAGTCGAGATAAGCCTGCACCGGGCAGAGGCACTTCAAGGCTGGTACGTAATGAGTCGTGCCGAGGTTGTCCCTGTCACCCTTAc > 1:133657/1‑150 (MQ=255) tGCCCCCAGCGATCCAGGCG‑ACAGAACACTGCACCACGGGCAATGCCGGCGGCACTGATCCAGTCGAGATAAGCCTGCACCGGGCAGAGGCACTTCAAGGCTGGTACGTAATGAGTCGTGCCGAGGTTGTCCCTGTCACCCTTACTTtgt < 2:58022/150‑1 (MQ=255) ccccAGCGATCCAGGCG‑ACAGAACACTGCACCACGGGCAATGCCGGCGGCACTGATCCAGTCGAGATAAGCCTGCACCGGGCAGAGGCACTTCAAGGCTGGTACGTAATGAGTCGTGCCGAGGTTGTCCCt > 1:790257/1‑131 (MQ=255) ccccAGCGATCCAGGCG‑ACAGAACACTGCACCACGGGCAATGCCGGCGGCACTGATCCAGTCGAGATAAGCCTGCACCGGGCAGAGGCACTTCAAGGCTGGTACGTAATGAGTCGTGCCGAGGTTGTCCCt < 2:790257/131‑1 (MQ=255) cccAGCGATCCAGGCG‑ACAGAACACTTCACCACGGGCAATGCCGGCGGCACTGATCCAGTCGAGATAAGCCTGCACCGGGCAGAGGCACTTCAAGGCTGGTACGTAATGAGTCGTGCCGAGGTTGTCCCTGTCAccc < 1:804211/137‑1 (MQ=255) cccAGCGATCCAGGCG‑ACAGAACACTGCACCACGGGCAATGCCGGCGGCACTGATCCAGTCGAGATAAGCCTGCACCGGGCAGAGGCACTTCAAGGCTGGTACGTAATGAGTCGTGCCGAGGTTGTCCCTGTCAccc > 2:804211/1‑137 (MQ=255) cccAGCGATCCAGGCG‑ACAGAACACTGCACCACGGGCAATGCCGGCGGCACTGATCCAGTCGAGATAAGCCTGCACCGGGCAGAGGCACTTCAAGGCTGGTACGTAATGAGTCGTGCCGAGGTTGTCCCTGTCACCCTTACTTTGTGGCa > 2:871883/1‑150 (MQ=255) aGCGATCCAGGCG‑ACAGAACACTGCACCACGGGCAATGCCGGCGGCACTGATCCAGTCGAGATAAGCCTGCACCGGGCAGAGGCACTTCAAGGCTGGTACGTAATGAGTCGTGCCGAGGTTGTCCCTGTCACCCTTACTTTGTGGCAGGa < 1:147763/150‑1 (MQ=255) gCGATCCAGGCG‑ACAGAACACTGCACCACGGGCAATGCCGGCGGCACTGATCCAGTCGAGATAAGCCTGCACCGGGCAGAGGCACTTCAAGGCTGGTACGTAATGAGTCGTGCCGAGGTTGTCCCTGTCACCCTTACTTTGTGGCAGGaa > 1:370679/1‑150 (MQ=255) cGATCCAGGCG‑ACAGAACACTGCACCACGGGCAATGCCGGCGGCACTGATCCAGTCGAGATAAGCCTGCACCGGGCAGAGGCACTTCAAGGCTGGTACGTAACGAGTCGTGCCGAGGTTGTCCCTGTCACCCTTACTTTGTGGCAGGAAc < 1:579829/150‑1 (MQ=255) gATCCAGGCG‑ACAGAACACTGCACCACGGGCAATGCCGGCGGCACTGATCCAGTCGAGATAAGCCTGCACCGGGCAGAGGCACTTCAAGGCTGGTACGTAATGAGTCGTGCCGAGGTTGTCCCTGTCACCCTTACTTtgtg > 2:752143/1‑141 (MQ=255) gATCCAGGCG‑ACAGAACACTGCACCACGGGCAATGCCGGCGGCACTGATCCAGTCGAGATAAGCCTGCACCGGGCAGAGGCACTTCAAGGCTGGTACGTAATGAGTCGTGCCGAGGTTGTCCCTGTCACCCTTACTTtgtg < 1:752143/141‑1 (MQ=255) ccAGGCG‑ACAGAACACTGCACCACGGGCAATGCCGGCGGCACTGATCCAGTCGAGATAAGCCTGCACCGTCCATAGGCACTTCAAGGCTGGTACGTAATGAGTCGTGCCGAGGTTGTCCCTGTCACCCTTACTTTGTGGCAGGAACAGGc < 1:747580/150‑1 (MQ=255) g‑ACAGAACACTGCACCACGGGCAATGCCGGCGGCACTGATCCAGTCGAGATAAGCCTGCACCGGGCAGAGGCACTTCAAGGCTGGTACGTAATGAGTCGTGCCGAGGTTGTCCCTGTCACCCTTACTTTGTGGCAGGAACAGGCTCATGc < 1:967607/150‑1 (MQ=255) g‑ACAGAACACTGCACCACGGGCAATGCCGGCGGCACTGATCCAGTCGAGATAAGCCTGCACCGGGCAGAGGCACTTCAAGGCTGGTACGTAACGAGTCGTGCCGAGGTTGTCCCTGTCACCCTTACTTTGTGGCAGGAACAGGCTCATGc > 2:752463/1‑150 (MQ=255) aGAACACTGCACCACGGGCAATGCCGGCGGCACTGATCCAGTCGAGATAAGCCTGCACCGGGCAGAGGCACTTCAAGGCTGGTACGTAATGAGTc < 2:100896/95‑1 (MQ=255) aGAACACTGCACCACGGGCAATGCCGGCGGCACTGATCCAGTCGAGATAAGCCTGCACCGGGCAGAGGCACTTCAAGGCTGGTACGTAATGAGTc > 1:100896/1‑95 (MQ=255) aGAACACTGCACCACGGGCAATGCCGGCGGCACTGATCCAGTCGAGATAAGCCTGCACCGGGCAGAGGCACTTCAAGGCTGGTACGTAATGAGTCGTGCCGAGGTTGTCCCTGTCACCCTTACTTTGTGGCAGGAACAgg > 2:1043255/1‑140 (MQ=255) aGAACACTGCACCACGGGCAATGCCGGCGGCACTGATCCAGTCGAGATAAGCCTGCACCGGGCAGAGGCACTTCAAGGCTGGTACGTAATGAGTCGTGCCGAGGTTGTCCCTGTCACCCTTACTTTGTGGCAGGAACAgg < 1:1043255/140‑1 (MQ=255) acacTGCACCACGGGCAATGCCGGCGGCACTGATCCAGTCGAGATAAGCCTGCACCGGGCAGAGGCACTTCAAGGCTGGTACGTAATGAGTCGTGCCGa > 2:713439/1‑99 (MQ=255) acacTGCACCACGGGCAATGCCGGCGGCACTGATCCAGTCGAGATAAGCCTGCACCGGGCAGAGGCACTTCAAGGCTGGTACGTAATGAGTCGTGCCGa < 1:713439/99‑1 (MQ=255) acTGCACCACGGGCAATGCCGGCGGCACTGATCCAGTCGAGATAAGCCTGCACCGGGCAGAGGCACTTCAAGGCTGGTACGTAATGAGTCGTGCCGAGGTTGTCCCTGTCACCCTTACTTTGTGGCAg < 2:72042/128‑1 (MQ=255) acTGCACCACGGGCAATGCCGGCGGCACTGATCCAGTCGAGATAAGCCTGCACCGGGCAGAGGCACTTCAAGGCTGGTACGTAATGAGTCGTGCCGAGGTTGTCCCTGTCACCCTTACTTTGTGGCAg > 1:72042/1‑128 (MQ=255) acTGCACCACGGGCAATGCCGGCGGCACTGATCCAGTCGAGATAAGCCTGCACCGGGCAGAGGCACTTCAAGGCTGGTACGTAATGAGTCGTGCCGAGGTTGTCCCTGTCACCCTTACTTTGTGGCAGGAACAGGCTCATGCCCGCACCg < 2:53392/150‑1 (MQ=255) accacGGGCAATGCCGGCGGCACTGATCCAGTCGAGATAAGCCTGCACCGGGCAGAGGCACTTCAAGGCTGGTACGTAATGAGTCGTGCCGAGGTTGTCCCTGTCACCCTTACTTTGTGGCAGGAACAGGCTCATGCCCGCACCGGCTTc < 2:772504/150‑1 (MQ=255) gCAATGCGGGCGGCACTGATCCAGTCGAGATAAGCGTGCACCGGGCAGAGGAACTTCAAGGCTGGTACGTAATGAGTCGTGCCGAGGTTGTCCCTGTCACCCTTACTTTGTGGCAGGAACAGGCTCATGCCCGCACCGGCTTCGGCCTgg < 1:828346/150‑1 (MQ=255) gCCGGCGGCACTGATCCAGTCGAGATAAGCCTGCACCGGGCAGAGGCACTTCAAGGCTGGTACGTAATGAGTCGTGCCGAGGTTGTCCCTGTCACCCTTACTTTGTGGCAGGAACAGGCTCATGGCGGCACCGTCTTCGGCCTGGATATg > 2:9977/1‑150 (MQ=255) ggcggcACTGATCCAGTCGAGATAAGCCTGCACCGGGCAGAGGCACTTCAAGGCTGGTACGTAATGAGTCGTGCCGAGGTTGTCCCTGTCACCCTTACTTTGTGGCAGGAACAGGCTCATGCCCGCACCTGTTTCGGCCTGGATATGTTc < 2:462579/150‑1 (MQ=255) tGATCCAGTCGAGATAAGCCTGCACCGGGCAGAGGCACTTCAAGGCTGGTACGTAATGAGTCGTGCCGAGGTTGTCCCTGTCACCCTTACTTTGTGGCAGGAACCGGCTCCTGACCGCACCGGCTTCGGCGTGGAAATGTTCGACCTGcc > 2:154957/1‑149 (MQ=255) tGATCCAGTCGAGATAAGCCTGCACCGGGCAGAGGCACTTCAAGGCTGGTACGTAATGAGTCGTGCCGAGGTTGTCCCTGTCACCCTTACTTTGTGGCAGGAACAGGCTCATGCCCGCACCGGCTTCGGCCTGGATATGTTCGACCTGCa > 2:382010/1‑150 (MQ=255) tCCAGTCGAGATAAGCCTGCACCGGGCAGAGGCACTTCAAGGCTGGTACGTAATGAGTCGTGCCGAGGTTGTCCCTGTCACCCTTACTTTGTGGCAGGAACAGGCTCATGCCCGCACCGGCTTCGGCCTGGa < 2:4303/132‑1 (MQ=255) tCCAGTCGAGATAAGCCTGCACCGGGCAGAGGCACTTCAAGGCTGGTACGTAATGAGTCGTGCCGAGGTTGTCCCTGTCACCCTTACTTTGTGGCAGGAACAGGCTCATGCCCGCACCGGCTTCGGCCTGGa > 1:4303/1‑132 (MQ=255) tCCAGTCGAGATAAGCCTGCACCGGGCAGAGGCACTTCAAGGCTGGTACGTAATGAGTCGTGCCGAGGTTGTCCCTGTCACCCTTACTTTGTGGCAGGAACAGGCTCATGCCCGCACCGGCTTCGGCCTGGATATGTTCGACCTGCAGcc > 1:463675/1‑150 (MQ=255) gTCGAGATAAGCCTGCACCGGGCAGAGGCACTTCAAGGCTGGTACGTAATGAGTCGTGCCGAGGTTGTCCCTGTCACCCTTACTTTGTGGCAGGAACAGGCTCATGCCCGCACCGGCTTCGGCCTGGATATGTTCGACCTGCAGCCGACa < 2:370679/150‑1 (MQ=255) aTAAGCCTGCACCGGGCAGAGGCACTTCAAGGCTGGTACGTAATGAGTCGTGCCGAGGTTGTCCCTGTCACCCTTACTTTGTGGCAGGAACAGGCTCATGCCCGCACCGGCTTCGGCCTGGATATGTTCGACCTGCAGCCGACAGAGTTc < 1:648917/150‑1 (MQ=255) ccTGCACCGGGCAGAGGCACTTCAAGGCTGGTACGTAATGAGTCGTGCCGAGGTTGTCCCTGTCACCCTTACTTTGTGGCAGGAACAGGc > 2:234213/1‑90 (MQ=255) ccTGCACCGGGCAGAGGCACTTCAAGGCTGGTACGTAATGAGTCGTGCCGAGGTTGTCCCTGTCACCCTTACTTTGTGGCAGGAACAGGc < 1:234213/90‑1 (MQ=255) ggCAGAGGCACTTCAAGGCTGGTACGTAATGAGTCGTGCCGAGGTTGTCCCTGTCACCCTTACTTTGTGGCAGGAACAGGCTCATGCCCGCACCGGCTTCGGCCTGGATATGTTCGACCTGCAGCCGACAGAGTTCGTCACTACGAAAAc < 1:9977/150‑1 (MQ=255) gCAGAGGCACTTCAAGGCTGGTACGTAATGAGTCGTGCCGAGGTTGTCCCTGTCACCCTTACTTTGTGGCAGGAACAGGCTCATGCCCGCa < 2:1086762/91‑1 (MQ=255) gCAGAGGCACTTCAAGGCTGGTACGTAATGAGTCGTGCCGAGGTTGTCCCTGTCACCCTTACTTTGTGGCAGGAACAGGCTCATGCCCGCa > 1:1086762/1‑91 (MQ=255) agagGCACTTCAAGGCTGGTACGTAATGAGTCGTGCCGAGGTTGTCCCTGTCACCCTTACTTTGTGGCAGGAACAGGCTCATGCCCGCACCGGCTTCGGCCTGGATATGTTCGACCTGCAGCCGACAGAGTTCGTCACTACGAAAAcccc < 2:463675/150‑1 (MQ=255) gCACTTCAAGGCTGGTACGTAATGAGTCGTGCCGAGGTTGTCCCTGTCACCCTTACTTTGTGGCAGGAACAGGCTCATGCCCGCACCGGCTTCGGCCTGGATATGTTCGACCTGCAGCCGACAGAGTTCGTCACTACGAAAACCCCGCCa < 2:747897/150‑1 (MQ=255) gCACTTCAAGGCTGGTACGTAATGAGTCGTGCCGAGGTTGTCCCTGTCACCCTTACTTTGTGGCAGGAACAGGCTCATGCCCGCACCGGCTTCGGCCTGGATATGTTCGACCTGCAGCCGACAGAGTTCGTCACTACGAAAACCCCGCCa < 2:133422/150‑1 (MQ=255) gCACTTCAAGGCTGGTACGTAATGAGTCGTGCCGAGGTTGTCCCTGTCACCCTTACTTTGTGGCAGGAACAGGCTCATGCCCGCACCGGCTTCGGCCTGGATATGTTCGACCTGCAGCCGACAGAGTTCGTCACTACGAAAACCCCGCCa < 2:133657/150‑1 (MQ=255) aaGGCTGGTACGTAACGAGTCGTGCCGAGGTTGTCCCTGTCACCCTTACTTTGTGGCAGGAACAGGCTCATGCCCGCACCGGCTTCGGCCTGGATATGTTCGACCTGCAGCCGACAGAGTTCGTCACTACGAAAACCCCGCCAGAAGCCg < 1:752463/150‑1 (MQ=255) gTAATGAGTCGTGCCGAGGTTGTCCCTGTCACCCTTACTTTGTGGCAGGAACAGGCTCATGCCCGCACCGGCTTCGGCCTGGATATGTTCGACCTGCAGCCGACAGAGTTCGTCACTACGAAAACCCCGCCAGAAGCCGATCAGCAGCaa > 2:973784/1‑150 (MQ=255) gTAATGAGTCGTGCCGAGGTTGTCCCTGTCACCCTTACTTTGTGGCAGGAACAGGCTCATGCCCGCACCGGCTTCGGCCTGGATATGTTCGACCTGCAGCCGACAGAGTTCGTCACTACGAAAACCCCGCCAGAAGCCGATCAGCAGCaa > 2:380165/1‑150 (MQ=255) tAATGAGTCGTGCCGAGGTTGTCCCTGTCACCCTTACTTTGTGGCAGGAACAGGCTCATGCCCGCACCGGCTTCGGCCTGGATATGTTCGACCTGCAGCCGACAGAGTTCGTCACTACGAAAACCCCGCCAGAAGCCGATCAGCAGCAAc < 1:319863/150‑1 (MQ=255) aaTGAGTCGTGCCGAGGTTGTCCCTGTCACCCTTACTTTGTGGCAGGAACAGGCTCATGCCCGCACCGGCTTCGGCCTGGATATGTTCGACCTGCAGCCGACAGAGTTCGTCACTACGAAAACCCCGCCAGAAGCCGATCAGCAGCAAcg > 1:183178/1‑150 (MQ=255) | TGATCAGGCTGCCGGGATGCAAGGCCTCGTCGCTCAGGTGCCCCCAGCGATCCAGGCG‑ACAGAACACTGCACCACGGGCAATGCCGGCGGCACTGATCCAGTCGAGATAAGCCTGCACCGGGCAGAGGCACTTCAAGGCTGGTACGTAATGAGTCGTGCCGAGGTTGTCCCTGTCACCCTTACTTTGTGGCAGGAACAGGCTCATGCCCGCACCGGCTTCGGCCTGGATATGTTCGACCTGCAGCCGACAGAGTTCGTCACTACGAAAACCCCGCCAGAAGCCGATCAGCAGCAACG > NC_002947/3379477‑3379773 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |