Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,380,583 T→A 12.7% L71Q (CTG→CAG)  tnpT‑I → cointegrate resolution protein T

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,380,5830TA12.7% 82.1 / 2.9 30L71Q (CTG→CAG) tnpT‑Icointegrate resolution protein T
Reads supporting (aligned to +/- strand):  ref base T (13/13);  new base A (2/2);  total (15/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGAGAACCCAAGCATCGACGCGGTACGGGTCGAACTTGGCAATACCGGCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGC  >  NC_002947/3380436‑3380728
                                                                                                                                                   |                                                                                                                                                 
gCGAGAACCCAAGCATCGACGCGGTACGGGTCGAACTTGGCAATACCGGCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTgg                                                                                                                                                 >  2:713582/1‑150 (MQ=255)
      aCCCAAGCATCGACGCGGTACGGGTCGAACTTGGCAATACCGGCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCGACCAGGTTGGcc                                                                                                                                           <  1:407857/150‑1 (MQ=255)
          aaGCATCGACGCGGTACGGGTCGAACTTGGCAATACCGGCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACt                                                                                                                                       >  2:216899/1‑150 (MQ=255)
            gCATCGACGCGGTACGGGTCGAACTTGGCAATACCGGCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTgg                                                                                                                                     <  1:713582/150‑1 (MQ=255)
                  aCGCGGTACGGGTCGAACTTGGCAATACCGGCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCTCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATc                                                                                                                               >  1:291131/1‑150 (MQ=255)
                  aCGCGGTACGGGTCGAACTTGGCAATACCGGCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATc                                                                                                                               <  1:216899/150‑1 (MQ=255)
                      ggTACGGGTCGAACTTGGCAATACCGGCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCt                                                                                                                           >  1:635934/1‑150 (MQ=255)
                            ggTCGAACTTGGAAATACCGGCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGACGCGATACCGCTGAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAgagga                                                                                                                     <  2:615691/150‑1 (MQ=255)
                                gAACTTGGCAATACCGGCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGAt                                                                                                                 <  1:76471/150‑1 (MQ=255)
                                   cTTGGCAATACCGGCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATgcg                                                                                                              <  1:397180/150‑1 (MQ=255)
                                    ttGGCAATACCGGCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATgcgc                                                                                                             >  1:2812/1‑150 (MQ=255)
                                      ggCAATACCGGCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAGCAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAg                                                                                                           <  1:340240/150‑1 (MQ=255)
                                      ggCAATACCGGCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAg                                                                                                           <  2:635934/150‑1 (MQ=255)
                                                 cTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCAAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGc                                                                                                <  2:247998/150‑1 (MQ=255)
                                                          caccaTTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCa                                                                                             >  1:1020981/1‑144 (MQ=255)
                                                          caccaTTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCa                                                                                             <  2:1020981/144‑1 (MQ=255)
                                                                 cACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGaacaa                                                                                <  2:301270/150‑1 (MQ=255)
                                                                          cTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGAGAACAGTTGACCAACCAGGTCGGCCAACTGGCAGATCAGCTAGACGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAAcgc                                                                       >  2:539206/1‑150 (MQ=255)
                                                                                            gCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAg                                                     >  2:1005457/1‑150 (MQ=255)
                                                                                                aGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGCTTACCAGAAcc                                                 <  1:894185/150‑1 (MQ=255)
                                                                                                     ggCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCt                                            >  1:380071/1‑150 (MQ=255)
                                                                                                      gCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCTAACGGCTCGATTACCAGAACCAAGCTc                                           <  2:323833/150‑1 (MQ=255)
                                                                                                         gAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGc                                        >  1:333001/1‑150 (MQ=255)
                                                                                                                                      cAGTTGGCCGACCAGGTTGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGaa           >  1:1007729/1‑150 (MQ=255)
                                                                                                                                      cAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGaa           <  1:1005457/150‑1 (MQ=255)
                                                                                                                                       aGTTGGCCGACCAGGTTGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGaaa          <  2:1007729/150‑1 (MQ=255)
                                                                                                                                            gCCAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCag     >  2:975608/1‑150 (MQ=255)
                                                                                                                                             ccAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCaga    >  1:117934/1‑150 (MQ=255)
                                                                                                                                               aaCCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGagc  >  2:812658/1‑150 (MQ=255)
                                                                                                                                               aaCCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGagc  >  2:93969/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GCGAGAACCCAAGCATCGACGCGGTACGGGTCGAACTTGGCAATACCGGCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGC  >  NC_002947/3380436‑3380728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑