| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,397,589 | C→T | 7.3% | G9G (GGC→GGT) | PP_3002 → | shikimate dehydrogenase‑like protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,397,589 | 0 | C | T | 7.3% | 151.3 / 2.0 | 55 | G9G (GGC→GGT) | PP_3002 | shikimate dehydrogenase‑like protein |
| Reads supporting (aligned to +/- strand): ref base C (23/28); new base T (2/2); total (25/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01 | |||||||||||
CAAGTAGCCCCGGACCTTGGCCAGCACAACGCCGAAGTGGCCGCCGGGCTTGGCTTCAGCCCTGAAGAAATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTACTGCAGTTTCGTAGCGAAGGCGGGAAGGGCAT > NC_002947/3397444‑3397738 | cAAGTAGCCCCGGACCTTGGCCAGCACAACGCCGAAGTGGCCGCCGGGCTTGGCTTCAGCCCTGAAGAAATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGccgac < 2:281791/150‑1 (MQ=255) aGTAGCCCCGGACCTTGGCCAGCACAACGCCGAAGTGGCCGCCGGGCTTGGCTTCAGCCCTGAAGAAATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGccgaccg > 2:621072/1‑150 (MQ=255) cccGGACCTTGGCCAGCACAACGCCGAAGTGGCCGCCGGGCTTGGCTTCAGCCCTGAAGAAATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAAc < 1:670545/150‑1 (MQ=255) cccGGACCTTGGCCAGCACAACGCCGAAGTGGCCGCCGGGCTAGGATTCAGCCCTGAAGAAATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAAc < 1:965768/150‑1 (MQ=255) ttGGCCAGCACAACGCCGAAGTGGCCGCCGGGCTTGGCTTCAGCCCTGAAGAAATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCaa > 2:739184/1‑150 (MQ=255) ttGGCCAGCACAACGCCGAAGTGGCCGCCGGGCTTGGCTTCAGCCCTGAAGAAATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCaa > 2:670632/1‑150 (MQ=255) ccAGCACAACGCCGAAGTGGCCGCCGGGCTTGGCTTCAGCCCTGAAGAAATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTcc > 2:259620/1‑150 (MQ=255) aaCGCCGAAGTGGCCGCCGGGCTTGGCTTCAGCAATGAAGAAATCACTGCCTTGCAGGCAGATGGAGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCACCGCTGa > 1:203516/1‑150 (MQ=255) cGAAGTGGCCGCCGGGCTTGGCTTCAGCCCTGAAGAAATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGCGATCGGCCGACCGATCAACCACACCGAGTCTCCGCTGATTCAt < 2:1007432/150‑1 (MQ=255) ggCCGCCGGGCTTGGCTTCAGCCCTGAAGAAATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTg < 2:343292/150‑1 (MQ=255) ccgccgGGCTTGGCTTCAGCCCTGAAGAAATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGtt < 1:904830/150‑1 (MQ=255) gccgGGCTTGGCTTCAGCCCTGAAGAAATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCg > 2:981149/1‑150 (MQ=255) gcttCAGCCCTGAAGAAATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCaa < 1:238154/114‑1 (MQ=255) gcttCAGCCCTGAAGAAATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCaa > 2:238154/1‑114 (MQ=255) cAGCCCTGAAGAAATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCacac > 2:598614/1‑107 (MQ=255) cAGCCCTGAAGAAATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCacac < 1:598614/107‑1 (MQ=255) ccTGAAGAAATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACcagc > 2:947575/1‑150 (MQ=255) tGAAGAAATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACcagcag < 2:931309/150‑1 (MQ=255) agaaATCACTGCCTTGCAGGCAGATGGCTTGTTGTTTACCCACGTAGATGCACTATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTg < 2:203516/150‑1 (MQ=255) aTCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCCGTTCGCCCAGGCCAGCAACCAGCAGCTGGAAt < 1:621072/150‑1 (MQ=255) tCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTg > 1:492850/1‑118 (MQ=255) tCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTg < 2:492850/118‑1 (MQ=255) gCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAg < 1:1070795/150‑1 (MQ=255) tGGCGTGTTGTTTACCCACGGAGATGCATGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGATGATTCATGGCCTGATCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCg > 2:583288/1‑150 (MQ=255) gtgtTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTgg > 1:405718/1‑150 (MQ=255) tgttgtTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGga < 1:947575/150‑1 (MQ=255) tgttgtTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTAACCGCGGATTCATGTCCTGGTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGga < 1:981149/150‑1 (MQ=255) gttTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGgacgac > 1:1064823/1‑150 (MQ=255) tttACCCACGGAGATGCACGATGAGCGACCGCTCCCCAGTGAGCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACt < 2:88080/150‑1 (MQ=255) ccACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCCGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGaa < 1:815208/150‑1 (MQ=255) ccACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGaa > 2:1033381/1‑150 (MQ=255) aCGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCa > 1:105721/1‑95 (MQ=255) aCGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCa < 2:105721/95‑1 (MQ=255) aTGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCa < 2:1075140/147‑1 (MQ=255) aTGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCa > 1:1075140/1‑147 (MQ=255) tGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTa > 1:181017/1‑150 (MQ=255) gCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTAc > 2:498595/1‑150 (MQ=255) aCGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTACTg < 2:1089505/150‑1 (MQ=255) cGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTACTGc < 2:349371/150‑1 (MQ=255) gATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTACTGCa > 1:822701/1‑150 (MQ=255) gACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTACTGCAGTTTCGt < 1:498595/150‑1 (MQ=255) ccGCTACGCAGTGATCCGCCGTCCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTACTGCAGTTTCGTAg < 2:255647/150‑1 (MQ=255) cGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTACTGCAGTTTCGTAGc < 1:583288/150‑1 (MQ=255) cGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTACTGCAGTTTCGTAGc < 1:1033381/150‑1 (MQ=255) cGCTACGCAGTCATCGGCCGGCGGAGCAACCACACGAAGGCCCCGCGGATTGATGGCCTGTTCTCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTACCGGCCGCTCGTTGGACGACCCCTAAGCCCAGGTACTACAGATTCGTAGc > 2:618890/1‑150 (MQ=255) cTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTACTGCAGTTTCGTAGCGa > 1:323366/1‑150 (MQ=255) cTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTACTGCAGTTTCGTAGCGa > 1:786390/1‑150 (MQ=255) gttGATCGGTCGGCCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTACTGCAGTTTCGTAGCGaa > 1:232203/3‑145 (MQ=255) gttGATCGGTCGGCCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTACTGCAGTTTCGTAGCGaa < 2:232203/143‑1 (MQ=255) aGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACgg > 2:429951/1‑87 (MQ=255) aGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACgg < 1:429951/87‑1 (MQ=255) aGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGcc > 2:352212/1‑121 (MQ=255) aGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGcc < 1:352212/121‑1 (MQ=255) ttGATCGGTCGGCCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCg > 2:645252/2‑102 (MQ=255) ttGATCGGTCGGCCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCg < 1:645252/101‑1 (MQ=255) aTCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTACTGCAGTTTCGTAGCGAAGGCGGGAAg < 1:739184/150‑1 (MQ=255) cGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTACTGCAGTTTCGTAGCGAAGGCGGGacgca < 1:588175/150‑5 (MQ=255) ccgaccgaTCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTACTGCAGTTTCGTAGCGAAGGCGGGAAGGGCAt > 1:50174/1‑150 (MQ=255) | CAAGTAGCCCCGGACCTTGGCCAGCACAACGCCGAAGTGGCCGCCGGGCTTGGCTTCAGCCCTGAAGAAATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTACTGCAGTTTCGTAGCGAAGGCGGGAAGGGCAT > NC_002947/3397444‑3397738 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |