| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,516,648 | A→C | 12.2% | intergenic (+645/‑490) | PP_5576 → / → PP_5578 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,516,648 | 0 | A | C | 12.2% | 112.6 / 11.0 | 50 | intergenic (+645/‑490) | PP_5576/PP_5578 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (26/17); new base C (3/3); total (30/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.95e-01 | |||||||||||
ATCCGCTGGGCCGCAAGACCACCTACGAATATCATCACCTGACCACGCTGGTGAACCAGGTCAGCTACCCGGATGGCAGCACCTGGCGAGCGCGCTACGACGACAAGGGCAACCTGCTCGCCGAATTCGATGCCCT‑CGGCCAGATGACCGAGTACCTGAACAGCGATGACGGCCTGCCGCACACCATCATCGATGCGACCTACAAGTCCAAGTACCTGTGGTGGAACACCCTGGCCCAGGTCGAACGCTATCAGGACTGCTCGGGCAAGAGCACGTACTACCGCTATGACGAG > NC_002947/3516505‑3516797 | aTCCGCTGGGCCGCAAGACCACCTACGAATATCATCACCTGACCACGCTGGTGAACCAGGTCAGCTACCCGGATGGCAGCACCTGGCGAGCGCGCTACGACGACAAGGGCAACCTGCTCGCCGAATTCGATGCCCT‑CGGCCAGATGACCg > 2:1093624/1‑150 (MQ=255) gcggtagtacgtgctcttgcccgagcagtcctgatagcgttcgacctgggccagggtgttccaccacaggtacttggacttgtaggtcgcatcgatgatggtgtgcggcaggccgtcatcgctgttcaggtaCT‑CGGCCAGATGACCGAg > 2:409339‑M1/133‑150 (MQ=255) gCCGCAAGACCACCTACGAATATCATCACCTGACCACGCTGGTGAACCAGGTCAGCTACCCGGATGGCAGCACCTGGCGAGCGCGCTACGACGACAAGGGCAACCTGCTCGCCGAATTCGATGCCCT‑CGGCCAGATGACCGAGTACCTGa > 2:377977/1‑150 (MQ=255) gACCACCTACGAATATCATCACCTGACCACGCTGGTGAACCAGGTCAGCTACCCGGATGGCAGCACCTGGCGAGCGCGCTACGACGACAAGGGCAACCTGCTCGCCGAATTCGATGCCCT‑CGGCCAGATGACCGAGTACCTGAACAGCGa > 2:218399/1‑150 (MQ=255) aaTATCATCACCTGACCACGCTGGTGAACCAGGTCAGCTACCCGGATGGCAGCACCTGGCGAGCGCGCTACGACGACAAGGGCAACCTGCTCGCCGAATTCGATGCCCT‑CGGCCAGATGACAGAGCACCTGAACAGCGATGACGACCTGc > 2:1106332/1‑150 (MQ=255) atcatcACCTGACCACGCTGGTGAACCAGGTCAGCTACCCGGATGGCAGCACCTGGCGAGCGCGCTACGACGACAAGGGCAACCTGCTCGCCGAATTCGATGCCAT‑CGGCCAGATGACCGAGTACCTGAACAGCGATGACGACCTGCCGc < 1:1106332/150‑1 (MQ=255) tcatcaCCTGACCACGCTGGTGAACCAGGTCAGCTACCCGGATGGCAGCACCTGGCGAGCGCGCTACGACGACAAGGGCAACCTGCTCGCCGAATTCGATGCCCT‑CGGCCAGATGACCGAGTACCTGAACAGCGATGACGGCCTGCCGca < 1:377977/150‑1 (MQ=255) tcaCCTGACCACGCTGGTGAACCAGGTCAGCTACCCGGATGGCAGCACCTGGCGAGCGCGCTACGACGACAAGGGCAACCTGCTCGCCGAATCC‑ATAAGTTGCGCGGAGGTGACCGAGTACCTGAACAGCGATGACGGCCTGCCGCacac > 2:870142/1‑150 (MQ=14) aCCACGCTGGTGAACCAGGTCAGCTACCCGGATGGCAGCACCTGGCGAGCGCGCTACGACGACAAGGGCAACCTGCTCGCCGAATTCGATGCCCT‑CGGCCAGATGACCGAGTACCTGAACAGCGATGACGGCCTGCCGCACACCatcatc < 1:218399/150‑1 (MQ=255) gAACCAGGTCAGCTACCCGGATGGTAGCACCTGGCGAGCGCGCTACGACGACAAGGGCAACCTGCTCGCCGAATTCGATGCCCT‑CGGCCAGATGACCGAGTACCTGAACAGCGATGACGGCCTGCCGCACACCATCATCGATGCGACCTa > 1:327287/1‑150 (MQ=255) ggTCAGCTACCCGGATGGCAGCACCTGGCGAGCGCGCTACGACGACAAGGGCAACCTGCTCGCCGAATTCGATGCCCT‑CGGCCAGATGACCGAGTACCTGAACAGCGATGACGGCCTGCCGCACACCATCATCGATGCGACCTACAAGTc > 2:536310/1‑150 (MQ=38) tCAGCTACCCGGATGGCAGCACCTGGCGAGCGCGCTACGACGACAAGGGCAACCTGCTCGCCGAATTCGATGCCCT‑CGGCCAGATGACCGAGTACCTGAACAGCGATGACGGCCTGCCGcaca > 2:508015/1‑123 (MQ=40) tCAGCTACCCGGATGGCAGCACCTGGCGAGCGCGCTACGACGACAAGGGCAACCTGCTCGCCGAATTCGATGCCCT‑CGGCCAGATGACCGAGTACCTGAACAGCGATGACGGCCTGCCGcaca < 1:508015/123‑1 (MQ=40) tCAGCTACCCGGATGGCAGCACCTGGCGAGCGCGCTACGACGACAAGGGCAACCTGCTCGCCGAATTCGATGCCCT‑CGGCCAGATGACCGAGTACCTGAACAGCGATGACGGCCTGCCGCACACCATCATCGATGCGACCTACAAGTCCa > 1:1104380/1‑150 (MQ=39) cAGCTACCCGGATGGCAGCACCTGGCGAGCGCGCTACGACGACAAGGGCAACCTGCTCGCCGAATTCGATGCCCT‑CGGCCAGATGACCGAGTACCTGAACAGCGATGACGGCCTGCCGCACACCATCATCGATGCGACCTACAAGTCCaa < 2:52718/150‑1 (MQ=38) ccGGATGGCAGCACCTGGCGAGCGCGCTACGACGACAAGGGCAACCTGCTCGCCGAATTCGATGCCCT‑CGGCCAGATGACCGAGTACCTGAACAGCGATGACGGCCTGCCGCACACCATCATCGATGCGACCTACAAGTCCAAGTACCtg > 1:63735/1‑150 (MQ=39) cTGGCGAGCGCGCTACGACGACAAGGGCAACCTGCTCGCCGAATTCGATGCCCT‑CGGCCAGATGACCGAGTACCTGAACAGCGATGACGGCCTGCCGCACACCATCATCGATGc < 2:148521/114‑1 (MQ=39) cTGGCGAGCGCGCTACGACGACAAGGGCAACCTGCTCGCCGAATTCGATGCCCT‑CGGCCAGATGACCGAGTACCTGAACAGCGATGACGGCCTGCCGCACACCATCATCGATGc > 1:148521/1‑114 (MQ=39) tGGCGAGCGCGCTACGACGACAAGGGCAACCTGCTCGCCGAATTCGATGCCCT‑CGGCCAGATGACCGAGTACCTGAACAGCGATGACGGCCTGCCGCACACCATCATCGATGCGACCTACAAGTCCAAGTACCTGTGGTGGAACACCCTg > 1:317667/1‑150 (MQ=35) ggCGAGCGCGCTACGACGACAAGGGCAACCTGCTCGCCGAATTCGATGCCCT‑CGGCCAGATGACCGAGTACCTGAACAGCGATGACGGCCTGCCGCACACCATCATCGATGCGACCTACAAGTCCAAGTACCTGTGGTGGAACACCCTgg < 1:1093624/150‑1 (MQ=34) aGCGCGCTACGACGACAAGGGCAACCTGCTCGCCGAATTCGATGCCCT‑CGGCCAGATGACCGAGTACCTGAACAGCGATGACGGCCTGCCGCACACCATCATCGATGCGACCTACAAGTCCAAGTACCTGTGGTGGAACACCCTGGCCCa < 2:63735/150‑1 (MQ=34) gcgcgcTACGACGACAAGGGCAACCTGCTCGCCGAATTCGATGCCCT‑CGGCCAGATGACCGAGTACCTGAACAGCGATGACGGCCTGCCGCACACCATCATCGATGCGACCTACAAGTCCAAGTACCTGTGGTGGAACACCCTGGCCCAg < 1:536310/150‑1 (MQ=34) cgcTACGACGACAAGGGCAACCTGCTCGCCGAATTCGATGCCCT‑CGGCCAGATGACCGAGTACCTGAACAGCGATGACGGCCTGCCGCACACCATCATCGATGCGACCTACAAGTCCAAGTACCTGTGGTGGAACACCCTGGCCCAGGTc > 2:462728/1‑150 (MQ=33) gacgacAAGGGCAACCTGCTCGCCGAATTCGATGCCCT‑CGGCCAGATGACCGAGTACCTGAACAGCGATGACGGCCTGCCGCACACCATCATCGATGCGACCTACAAGTCCAAGTACCTGTGGTGGAACACCCTGGCCCAGGTCGAACGc > 1:515150/1‑150 (MQ=32) gacgacAAGGGCAACCTGCTCGCCGAATTCGATGCCCT‑CGGCCAGATGACCGAGTACCTGAACAGCGATGACGGCCTGCCGCACACCATCATCGATGCGACCTACAAGTCCAAGTACCTGTGGTGGAACACCCTGGCCCAGGTCGAACGc > 2:72336/1‑150 (MQ=32) aaGGGCAACCTGCTCGCCGAATTCGATGCCCT‑CGGCCAGATGACCGAGTACCTGAACAGCGATGACGGCCTGCCGCACACCATCATCGATGCGACCTACAAGTCCAAGTACCTGTGGTGGAACACCCTGGCCCAGGTCGaa > 2:420222/1‑141 (MQ=11) aaGGGCAACCTGCTCGCCGAATTCGATGCCCT‑CGGCCAGATGACCGAGTACCTGAACAGCGATGACGGCCTGCCGCACACCATCATCGATGCGACCTACAAGTCCAAGTACCTGTGGTGGAACACCCTGGCCCAGGTCGaa < 1:420222/141‑1 (MQ=11) aaGGGCAACCTGCTCGCCGAATTCGATGCCCT‑CGGCCAGATGACCGAGTACCTGAACAGCGATGACGGCCTGCCGCACACCATCATCGATGCGACCTACAAGTCCAAGTACCTGTGGTGGAACACCCTGGCCCAGGTCGAACGCTATCAg < 2:974416/150‑1 (MQ=35) cAACCTGCTCGCCGAATTCGATGCCCT‑CGGCCAGATGACCGAGTACCTGAACAGCGATGACGGCCTGCCGCACACCATCATCGATGCGACCTACAAGTCCAAGTACCTGTGGTGGAACACCCTGGCCCAGGTCGAACGCTATCAGGACTg < 2:1104380/150‑1 (MQ=35) ccTGCTCGCCGAATTCGATGCCCT‑CGGCCAGATGACCGAGTACCTGAACAGCGATGACGGCCTGCCGCACACCATCATCGATGCGACCTACAAGTCCAAGTACCTGTGGTGGAACACCCTGGCCCAGGTCGAACGCTATCAGGACTGCTc > 2:519524/1‑150 (MQ=35) ccTGCTCGCCGAATTCGATGCCCT‑CGGCCAGATGACCGAGTACCTGAACAGCGATGACGGCCTGCCGCACACCATCATCGATGCGACCTACAAGTCCAAGTACCTGTGGTGGAACACCCTGGCCCAGGTCGAACGCTATCAGGACTGCTc > 2:66891/1‑150 (MQ=35) cTGCTCGCCGAATTCGATGCCCT‑CGGCCAGATGACCGAGTACCTGAACAGCGATGACGGCCTGCCGCACACCATCATCGATGCGACCTACAAGTCCAAGTACCTGTGGTGGAACACCCTGGCCCAGGTCGAACGCTATCAGGACTGCTCg > 1:721471/1‑150 (MQ=35) tCGCCGAATTCGATGCCCT‑CGGCCAGATGACCGAGTACCTGAACAGCGATGACGGCCTGCCGCACACCATCATCGATGCGACCTACAAGTCCAAGTACCTGTGGTGGAACACCCTGGCCCAGGTCGAACGCTATCAGGa > 2:720795/1‑139 (MQ=35) tCGCCGAATTCGATGCCCT‑CGGCCAGATGACCGAGTACCTGAACAGCGATGACGGCCTGCCGCACACCATCATCGATGCGACCTACAAGTCCAAGTACCTGTGGTGGAACACCCTGGCCCAGGTCGAACGCTATCAGGa < 1:720795/139‑1 (MQ=35) gCCGAATTCGATGCCCT‑CGGCCAGATGACCGAGTACCTGAACAGCGATGACGGCCTGCCGCACACCATCATCGATGCGACCTACAAGTCCAAGTACCTGTGGTGGAACACCCTGGCCCAGGTCGAACGCTATCAGGACTGCTCGGGCAag > 1:438646/1‑150 (MQ=35) gCCGAATTCGATGCCCT‑CGGCCAGATGACCGAGTACCTGAACAGCGATGACGGCCTGCCGCACACCATCATCGATGCGACCTACAAGTCCAAGTACCTGTGGTGGAACACCCTGGCCCAGGTCGAACGCTATCAGGACTGCTCGGGCAag < 1:470501/150‑1 (MQ=35) gCCGAATTCGATGCCCT‑CGGCCAGATGACCGAGTACCTGAACAGCGATGACGGCCTGCCGCACACCATCATCGATGCGACCTACAAGTCCAAGTACCTGTGGTGGAACACCCTGGCCCAGGTCGAACGCTATCAGGACTGCTCGGGCAag > 1:807544/1‑150 (MQ=35) tcgctgttcaggtaCT‑CGGTCATCTGGCCGAGTACCTGAACAGCGATGACGGCCTGCCGCACACCATCATCGATGCGACCTa > 2:902940‑M1/15‑82 (MQ=255) tcgctgttcaggtaCT‑CGGTCATCTGGCCGAGTACCTGAACAGCGATGACGGCCTGCCGCACACCATCATCGATGCGACCTa < 1:902940‑M1/68‑1 (MQ=255) cGATGCCCT‑CGGCCAGATGACCGAGTACCTGAACAGCGATGACGGCCTGCCGCACACCATCATCGATGCGACCTACAAGTCCAAGTACCTGTGGTGGAACACCCTGGCCCAGGTCGAACGCTATCAGGACTGCTCGGGCAAGAGCACGta < 1:66891/150‑1 (MQ=39) gtaCT‑CGGTCATCTGGCCGAGTACCTGAACAGCGATGACGGCCTGCCGCACACCATCATCGa < 1:704525‑M1/59‑1 (MQ=255) gtaCT‑CGGTCATCTGGCCGAGTACCTGAACAGCGATGACGGCCTGCCGCACACCATCATCGa > 2:704525‑M1/4‑62 (MQ=255) gtaCT‑CGGTCATCTGGCCGAGTACCTGAACAGCGATGACGGCCTGCCGCACACCATCATCGATGCGACCTACAAGTCCAAGTACCTGTGGTGGAACACCCTGGCCCAGGTCGaa > 2:865137‑M1/4‑114 (MQ=255) gtaCT‑CGGTCATCTGGCCGAGTACCTGAACAGCGATGACGGCCTGCCGCACACCATCATCGATGCGACCTACAAGTCCAAGTACCTGTGGTGGAACACCCTGGCCCAGGTCGaa < 1:865137‑M1/111‑1 (MQ=255) CGGCCAGATGACCGAGTACCTGAACAGCGATGACGGCCTGCCGCACACCATCATCGATGCGACCTACAAGTCCAAGTACCTGTGGTGGAACACCCTGGCCCAGGTCGAACGCTATCAGGACTGCTCGGGCAAGAGCACGTACTACCGCTa < 1:661719‑M1/150‑1 (MQ=255) ccAGATGACCGAGTACCTGAACAGCGATGACGGCCTGCCGCACACCATCATCGATGCGACCTACAAGTCCAAGTACCTGTGGTGGAACACCCTGGCCCAGGTCGAACGCTATCAGGACTGCTCGGGCAAGAGCACGTACTACCGCTATGa > 1:553078/1‑150 (MQ=39) cAGATGACCGAGTACCTGAACAGCGATGACGGCCTGCCGCACACCATCATCGATGCGACCTACGAGTCCAAGTACCTGTGGTGGAACACCCTGGCCCAGGTCGAACGCTATCAGGACTGCTCGGGCAAGAGCACGTACTACCGCTATGAc > 1:27927/1‑150 (MQ=38) cAGATGACCGAGTACCTGAACAGCGATGACGGCCTGCCGCACACCATCATCGATGCGACCTACAAGTCCAAGTACCTGTGGTGGAACACCCTGGCCCAGGTCGAACGCTATCAGGACTGCTCGGGCAAGAGCACGTACTACCGCTATGAc < 2:327287/150‑1 (MQ=39) cAGATGACCGAGTACCTGAACAGCGATGACGGCCTGCCGCACACCATCATCGATGCGACCTACAAGTCCAAGTACCTGTGGTGGAACACCCTCGCCCAGGTCGAACGCTATCAGGACTGCTCGGGCAAGAGCACGTACTACCGCTATGAc > 2:152993/1‑150 (MQ=38) gATGACCGAGTACCTGAACAGCGATGACGGCCTGCCGGACACCATCATCGATGCGACCTACAAGTCCAAGTACCTGTGGTGGAACACCCTGGCCCAGGTCGAACGCTATCAGGACTGCTCGGGCAAGAGCACGTACTACCGCTATGACGa > 2:244646/1‑150 (MQ=38) aTGACCGAGTACCTGAACAGCGATGACGGCCTGCCGCACACCATCATCGATGCGACCTACAAGTCCAAGTACCTGTGGTGGAACACCCTGGCCCAGGTCGAACGCTATCAGGACTGCTCGGGCAAGAGCACGTACTACCGCTATGACGAg < 2:661719/150‑1 (MQ=40) | ATCCGCTGGGCCGCAAGACCACCTACGAATATCATCACCTGACCACGCTGGTGAACCAGGTCAGCTACCCGGATGGCAGCACCTGGCGAGCGCGCTACGACGACAAGGGCAACCTGCTCGCCGAATTCGATGCCCT‑CGGCCAGATGACCGAGTACCTGAACAGCGATGACGGCCTGCCGCACACCATCATCGATGCGACCTACAAGTCCAAGTACCTGTGGTGGAACACCCTGGCCCAGGTCGAACGCTATCAGGACTGCTCGGGCAAGAGCACGTACTACCGCTATGACGAG > NC_002947/3516505‑3516797 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |