Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,644,600 C→T 9.1% A117T (GCC→ACC)  PP_3211 ← ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,644,6000CT 9.1% 117.6 / 3.3 44A117T (GCC→ACC) PP_3211ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (16/24);  new base T (2/2);  total (18/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.48e-01

GCATACCGCCGGAGAGCTCCCGCGGGTAAACGTCGCCGAACTTGCCCAGCCCCACCAGTTCCAGGGCGGCTTGCACCTTGAGCTGGCTCTGGGCCTTGGGGGCGCCGGCCAAGTCCAGGGGCAAGCGCACGTTGTCGTGCACTTTGGCCCATGGCATCAGGGTGGCTTCCTGGAACACCATGGCCAGGCTGCGCCCCGGTGTGCCTGCGTGGTGGTCCGTACCCGGGGCATCCTTGCCCCACCAGCGCACATGGCCGGCGGAGGGCTGTTCGAGCCCGGCGAACATTTTCAGC  >  NC_002947/3644453‑3644745
                                                                                                                                                   |                                                                                                                                                 
gCATACCGCCGGAGAGCTCCCGCGGGTAAACGTCGCCGAACTTGCCCAGCCCCACCAGTTCCAGGGCGGCTTGCACCTTGAACTGCCTCTGGTCCTTGGGGGCGCCGGCCAAGTCCAGTGGCAAGCGCACGTTGTCGTGCACTTTGGccc                                                                                                                                                 >  2:505100/1‑150 (MQ=255)
    aCCGCCGGAGAGCTCCCGCGGGTAAACGTCGCCGAACTTGCCCAGCCCCACCAGTTCCAGGGCGGCTTGCACCTTGAGCTGGCTCTGGGCCTTGGGGGCGCCGGCCAAGTCCAGGGGCAAGCGCACGTTGTCGTGCACTTTGGCCCATgg                                                                                                                                             >  2:647137/1‑150 (MQ=255)
              ggCTCCCGCGGGTAAACGTCGCCGCACTTGCCCAGCCCCACCAGTTCCAGGGCGGATTGCACCGTGGGCTGGCTCTGGGCCTTGGGGGCGCCGGCCAAGTCCAGGGGCCAGCGCACGTTGTCGCGCACTTTGGCCCATGGCATCAGGGTg                                                                                                                                   <  2:564293/149‑1 (MQ=255)
              agCTCCCGCGGGTAAACGTCGCCTAACTTGCCCAGCCCCACCAGTTCCAGGGCGGCTTGCACCTTGAGCTGGCTCTGGGCCTTGGGGGCGCCGGCCAAGTCCAGGGGCAAGTGCACGTTGTCGTGCACTTTGGCCCATGGCATCAGGGTg                                                                                                                                   <  1:146044/150‑1 (MQ=255)
                          tAAACGTCGCCGAACTTGCCCAGCCCCACCAGTTCAACAACGGTTTGCACCTTCAGATGCCTCTGGGCCTTGGGGGCGCCGGCCAAGTCCAGGGGCAAACGCACGTTGTCGTGCACTTTAGCCCCTGGCATCAGGCGGGCTTCCTGCAac                                                                                                                       >  2:39005/1‑150 (MQ=255)
                               gTCGCCGAACTTGCCCAGCCCCACCAGTTCCAGGGCGGCTTGCACCTTGAGCTGGCTCTGGGCCTTGGGGGCGCCGGCCAAGTCCAGGGGCAAGCGCACGTTGTCGTGCACTTTGGCCCATGGCATCAGGGTGGCTTCCTGGAACAccct                                                                                                                  <  1:813263/150‑3 (MQ=255)
                               gTCGCCGAACTTGCCCAGCCCCACCAGTTCCAGGGCGGCTTGCACCTTGAGCTGGCTCTGGGCCTTGGGGGCGCCGGCCAAGCCCAGGGGCAAGCGCACGTTGTCGTGCACTTTGGCCCATGGCATCAGGGTGGCTTCCTGGAACACCAt                                                                                                                  <  1:905506/150‑1 (MQ=255)
                                  gCCGAACTTGCCCAGCCCCACCAGTTCCAGGGCGGCTTGCACCTTGAGCTGGCTCTGGGCCTTGGGGGCGCCGGCCAAGTCCAGGGGCAAGCGCACGTTGTCGTGCACTTTGGCCCATGGCATCAGGGTGGCTTCCTGGAACACCATGGc                                                                                                               <  2:950282/150‑1 (MQ=255)
                                  gCCGAACTTGCCCAGCCCCACCAGTTCCAGGGCGGCTTGCACCTTGAGCTGGCTCTGGGCCTTGGGGGCGCCGGCCAAGTCCAGGGGCAAGCGCACGTTGTCGTGCACTTTGGCCCATGGCATCAGGGTGGCTTCCTGGAACACCATGGc                                                                                                               >  2:1099983/1‑150 (MQ=255)
                                  gCCGAACTTGCCCAGCCCCACCAGTTCCAGGGCGGCTTGCACCTTGAGCTGGCTCTGGGCCTTGGGGGCGCCGGCCAAGTCCAGGGGCAAGCGCACGTTGTCGTGCACTTTGGCCCATGGCATCAGGGTGGCTTCCTGGAACACCATGGc                                                                                                               >  1:950282/1‑150 (MQ=255)
                                       aCTTGCCCAGCCCCACCAGTTCCAGGGCGGCTTGCACCTTGAGCTGGCTCTGGGCCTTGGGGGCGCCGGCCAAGTCCAGGGGCAAGCGCACGTTGTCGTGCACTTTGGCCCATGGCATCAGGGTGGCTTCCTGGAACACCATGGCCAGGc                                                                                                          <  1:497164/150‑1 (MQ=255)
                                        cTTGCCCAGCCCCACCAGTTCCAGGGCGGCTTGCACCTTGAGCTGGCTCTGGGCCTTGGGGGCGCCGGCCAAGTCCAGGGGCAAGCGCACGTTGTCGTGCACTTTGGCCCATGGCATCAGGGTGGCTTCCTGGAACACCATGGCCAGGCt                                                                                                         <  2:742557/150‑1 (MQ=255)
                                             ccAGCCCCACCAGTTCCAGGGCGGCTTGCACCTTGAGCTGGCTCTGGGCCTTGGGGGCGCCGGCCAAGTCCATGGGCAAGCGCACGTTGTCGTGCACTTTGGCCCATGGCATCAGGGTGGCTTCCTGGAACACCATGGCCAGGCTGCGcc                                                                                                    >  2:610031/1‑150 (MQ=255)
                                                gCCCCACCAGTTCCAGGGCGGCTTGCACCTTGAGCTGGCTCTGGGCCTTGGGGGCGCCGGCCAAGTCCAGGGGCAAGCGCACGTTGTCGTGCACTTTGGCCCATGGCATCAGGGTGGCTTCCTGGAACACCATGGCCAGGCTGCGCCCCg                                                                                                 <  2:1042930/150‑1 (MQ=255)
                                                     accaGTTCCAGGGCGGCTTGCACCTTGAGCTGGCTCTGGGCCTTGGGGGCGCCGGCCAAGTCCAGGGGCAAGCGCACGTTGTCGTGCACTTTGGCCCATGGCATCAGGGTGGCTTCCTGGAACACCATGGCCAGGCTGCGCCCCGGtgtg                                                                                            <  1:1099983/150‑1 (MQ=255)
                                                      ccaGTTCCAGGGCGGCTTGCACCTTGAGCTGGCTCTGGGCCTTGGGGGCGCCGGCCAAGTCCAGGGGCAAGCGCACGTTGTCGTGCACTTTGGCCCATGGCATCAGGGTGGCTTCCTGGAACACCATGGCCAGGCTGCGCCCCGGTGTGc                                                                                           <  1:136933/150‑1 (MQ=255)
                                                         gTTCCAGGGCGGCTTGCACCTTGAGCTGGCTCTGGGCCTTGGGGGCGCCGGCCAAGTCCAGGGGCAAGCGCACGTTGTCGTGCACTTTGGCCCATGGCATCAGGGTGGCTTCCTGGAACACCATGGCCAGGCTGCGCCCCGGTGTGCCTg                                                                                        >  2:320810/1‑150 (MQ=255)
                                                             cAGGGCGGCTTGCACCTTGAGCTGGCTCTGGGCCTTGGGGGCGCCGGCCAAGTCCAGGGGCAAGCGCACGTTGTCTTGCACTTTGGCCCATGGCATCAGGGTGGCTTCCTGGAACACCATGGCCAGGCTGCGCCCCGGTGTGCCTGCgtg                                                                                    <  2:334732/150‑1 (MQ=255)
                                                              aGGGCGGCTTGCACCTTGAGCTGGCTCTGGGCCTTGGGGGCGCCGGCCAAGTCCAGGGGCAAGCGCACGTTGTCGTGCACTTTGGCCCATGGCATCAGGGTGGCTTCCTGGAACACCATGGCCAGGCTGCGCCCCGGTGTGCCGGCgtgg                                                                                   <  2:883807/150‑1 (MQ=255)
                                                                    gcTTGCACCTTGAGCTGGCTCTGGGCCTTGGGGGCGCCGGCCAAGTCCAGGGGCAAGCGCACGTTGTCGTGCACTTTGGCCCATGGCATCAGGGTGGCTTCCTGGAACACCATGGCCAGGCTGCGCCCCGGTGTGCCTGCGTGGTGGTcc                                                                             <  2:557530/150‑1 (MQ=255)
                                                                                     gCTCTGGGCCTTGGGGGCGCCGGCCAAGTCCAGGGGCAAGCGCACGTTGTCGTGCACTTTGGCCCATGGCATCa                                                                                                                                        >  1:320455/1‑74 (MQ=255)
                                                                                     gCTCTGGGCCTTGGGGGCGCCGGCCAAGTCCAGGGGCAAGCGCACGTTGTCGTGCACTTTGGCCCATGGCATCa                                                                                                                                        <  2:320455/74‑1 (MQ=255)
                                                                                       tctGGGCCTTGGGGGCGCCGGCCAAGTCCAGGGGCAAGCGCACGTTGTCGTGCACTTTGGCCCATGGCATCAGGGTGGCTTCCTGGAACACCATGGCCAGGCTGCGCCCCGGTGTGCCTGCGTGGTGGTCCGTACCCGGGGCATCCTTGc                                                          <  2:781196/150‑1 (MQ=255)
                                                                                        ctGGGCCTTGGGGGCGCCGGCCAAGTCCAGGGGCAAGCGCACGTTGTCGTGCACTTTGGCCCATGGCATCAGGGTGGCTTCCTGGAACACCATGGCCAGGCTGCGCCCCGGTGTGCCTGCGTGGTGGTCCGTACCCGGGGCATCCTTGcc                                                         <  1:647137/150‑1 (MQ=255)
                                                                                             ccTTGGGGGCGCCGGCCAAGTCCAGGGGCAAGCGCACGTTGTCGTGCACTTTGGCCCATGGCATCAGGGTGGCTTCCTGGAACACCATGGCCAGGCTGCGCCCCGGTGTGCCTGCGTGGTGGTCCGTACCCGGGGCATCCTTGCCccacc                                                    >  1:1941/1‑150 (MQ=255)
                                                                                             ccTTGGGGGCGCCGGCCAAGTCCAGGGGCAAGCGCACGTTGTCGTGCACTTTGGCCCATGGCATCAGGGTGGCTTCCTGGAACACCATGGCCAGGCTGCGCCCCGGTGTGCCTGCGTGGTGGTCCGTACCCGGGGCATCCTTGCCccacc                                                    >  2:147093/1‑150 (MQ=255)
                                                                                              cTTGGGGGCGCCGGCCAAGTCCAGGGGCAAGCGCACGTTGTCGTGCACTTTGGCCCATGGCATCAGGGTGGCTTCCTGGAACACCATGGCCAGGCTGCGCCCCGGTGTGCCTGCGTGGTGGTCCGTACCCGGGGCATCCTTGCCccacca                                                   <  1:59494/150‑1 (MQ=255)
                                                                                                   gggCGCCGGCCAAGTCCAGGGGCAAGCGCACGTTGTCGTGCACTTTGGCCCATGGCATCAGGGTGGCTTCCTGGAACACCATGGCCAGGCTGCGCCCCGGTGTGCCTGCGTGGTGGTCCGTACCCGGGGCATCCTTGCCCCACCAGCGca                                              <  1:858281/150‑1 (MQ=255)
                                                                                                    ggCGCCGGCCAAGTCCAGGGGCAAGCGCACGTTGTCGTGCACTTTGGCCCATGGCATCAGGGTGGCTTCCTGGAACACCATGGCCAGGCTGCGCCCCGGTGTGCCTGCGTGGTGGTCCGTACCCGGGGCATCCTTGCCCCACCAGCGcac                                             <  2:1009100/150‑1 (MQ=255)
                                                                                                       gcCGGCCAAGTCCAGGGGCAAGCGCACGGTGTCGTGCACTTTGGCCCATGGCATCAGGGTGGCTTCCTGGAACACCATGGCCAGGCTGCGCCCCGGTGTGCCTGCGTGGTGGTCCGTACCCGGGGCATCCTTGCCCCACCAGCGCACATg                                          >  1:262019/1‑150 (MQ=255)
                                                                                                         cGGCCAAGTCCAGGGGCAAGCGCACGTTGTCGTGCACTTTGGCCCATGGCATCAGGGTGGCTTCCTGGAACACCATGGCCAGGCTGCGCCCCGGTGTGCCTGCGTGGTGGTCCGTACCCGGGGCATCCTTGCCCCACCAGCGCACATGGc                                        >  1:422122/1‑150 (MQ=255)
                                                                                                            ccAAGTCCAGGGGCAAGCGCACGTTGTCGTGCACTTTCGCCCATGGCATCAGGGTGGCTTCCTGGAACACCATGGCCAGACTGCGCCCCGGTGTGCCTGCGTGGTGGTCCGTACCCGGGGCATCCTTGCCCCACCAGCGCACATGGCcgg                                     >  1:366462/1‑150 (MQ=255)
                                                                                                             cAAGTCCAGGGGCAAGCGCACGTTGTCGTGCACTTTGGCCCATGGCATCAGGGTGGCTTCCTGGAACACCATGGCCAGGCTGCGCCCCGGTGTGCCTGCGTGGTGGTCCGTACCCGGGGCATCCTTGCCCCACCAGCGCACATGGCcggc                                    >  2:811965/1‑150 (MQ=255)
                                                                                                                    aGGGGCAAGCGCACGTTGTCGTGCACTTTGGCCCATGGCATCAGGGTGGCTTCCTGGAACACCATGGCCAGGCTGCGCCCCGGTGTGCCTGCGTGGTGGTCCGTACCCGGGGCATCCTTGCCCCACCAGCGCACATGGCCGGCGGAGGGc                             >  1:530874/1‑150 (MQ=255)
                                                                                                                      gggCAAGCGCACGTTGTCGTGCACTTTGGCCCATGGCATCAGGGTGGCTTCCTGGAACACCATGGCCAGGCTGCGCCCCGGTGTGCCTGCGTGGTGGTCCGTACCCGGGGCATCCTTGCCCCACCAGCGCACATGGCCGGCGGAGGGCTg                           >  2:472115/1‑150 (MQ=255)
                                                                                                                         cAAGCGCACGTTGTCGTGCACTTTGGCCCATGGCATCAGGGTGGCTTCCTGGAACACCATGGCCAGGCTGCGCCCCGGTGTGCCTGCGTGGTGGTCCGTACCCGGGGCATCCTTGCCCCACCAGCGCACATGGCCGGCGGAGGGCTGTTc                        <  2:422122/150‑1 (MQ=255)
                                                                                                                              gcACGTTGTCGTGCACTTTGGTCCATGGCATCAGGGTGGCTTCCTGGAACACCATGGCCAGGCTGCGCCCCGGTGTGCCTGCGTGGTGGTCCGTAc                                                                         >  2:1117740/1‑96 (MQ=255)
                                                                                                                              gcACGTTGTCGTGCACTTTGGTCCATGGCATCAGGGTGGCTTCCTGGAACACCATGGCCAGGCTGCGCCCCGGTGTGCCTGCGTGGTGGTCCGTAc                                                                         >  2:1117442/1‑96 (MQ=255)
                                                                                                                              gcACGTTGTCGTGCACTTTGGTCCATGGCATCAGGGTGGCTTCCTGGAACACCATGGCCAGGCTGCGCCCCGGTGTGCCTGCGTGGTGGTCCGTAc                                                                         <  1:1117740/96‑1 (MQ=255)
                                                                                                                              gcACGTTGTCGTGCACTTTGGTCCATGGCATCAGGGTGGCTTCCTGGAACACCATGGCCAGGCTGCGCCCCGGTGTGCCTGCGTGGTGGTCCGTAc                                                                         <  1:1117442/96‑1 (MQ=255)
                                                                                                                              gcACGTTGTCGTGCACTTTGGCCCATGGCATCAGGGTGGCTTCCTGGAACACCATGGCCAGGCTGCGCCCCGGTGTGCCTGCgtggtg                                                                                 <  2:22635/88‑1 (MQ=255)
                                                                                                                              gcACGTTGTCGTGCACTTTGGCCCATGGCATCAGGGTGGCTTCCTGGAACACCATGGCCAGGCTGCGCCCCGGTGTGCCTGCgtggtg                                                                                 >  1:22635/1‑88 (MQ=255)
                                                                                                                                    tGTCGTGCACTTTGGCCCATGGCATCAGGGTGGCTTCCTGGAACACCATGGCCAGGCTGCGCCCCGGTGTGCCTGCGTGGTGGTCCGTACCCGGGGCATCCTTGCCCCACCAGCGCACATGGCCGGCGGAGGGCTGTTCGAGCCCGGCGa             <  1:610031/150‑1 (MQ=255)
                                                                                                                                    tGTCGTGCACTTTGGCCCATGGCATCAGGGTGGCTTCCTGGAACACCATGGCCAGGCTGCGCCCCGGTGTGCCTGCGTGGTGGTCCGTACCAGAAGACGGCTTGCCCGATGACTCCTCGTGGCTGGCGTAGGGCTGTTCGAGCCCGGCGa             <  1:139495/150‑1 (MQ=255)
                                                                                                                                               ttGGCCCATGGCATCAGGGTGGCTTCCTGGAACACCATGGCCAGGCTGCGCCCCGGTGTGCCTGCGTGGTGGTCCGTACCCGGGGCATCCTTGCCCCACCAGCGCACATGGCCGGCGGAGGGCTGTTCGAGCCCGGCGAACATTTTcagc  <  1:472115/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GCATACCGCCGGAGAGCTCCCGCGGGTAAACGTCGCCGAACTTGCCCAGCCCCACCAGTTCCAGGGCGGCTTGCACCTTGAGCTGGCTCTGGGCCTTGGGGGCGCCGGCCAAGTCCAGGGGCAAGCGCACGTTGTCGTGCACTTTGGCCCATGGCATCAGGGTGGCTTCCTGGAACACCATGGCCAGGCTGCGCCCCGGTGTGCCTGCGTGGTGGTCCGTACCCGGGGCATCCTTGCCCCACCAGCGCACATGGCCGGCGGAGGGCTGTTCGAGCCCGGCGAACATTTTCAGC  >  NC_002947/3644453‑3644745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑