Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,754,989 G→A 8.5% L129L (TTG→TTA)  PP_3319 → GGDEF domain‑containing sensory box protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,754,9890GA 8.5% 123.6 / 2.7 47L129L (TTG→TTA) PP_3319GGDEF domain‑containing sensory box protein
Reads supporting (aligned to +/- strand):  ref base G (20/23);  new base A (2/2);  total (22/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

CTTGCCCATCGGGCCTCAGGAAGGTACGTGTGGCACAGCTGCATTCCGCCGCGAGCTGGTGGTGACAGCGGATATAGCCCGGGATCCGAGCTGGGAGCGTTTTCGCAGTCTGGCGCTAGGGTACAATCTACGCTCCTGCTGGTCGTTGCCATTACTCTCGCATGAGGGCTCCGTTCTTGGGACATTTGCGCTATACCAGGATCAAGCGATTGCGCCAAGCGAGGTGCAGATCCATCGGCTGACCTGCGCGGCGCAACTGGCTGCCATTGCGATCCGGCATGAGCGTGACGGCCAAC  >  NC_002947/3754842‑3755137
                                                                                                                                                   |                                                                                                                                                    
cTTGCCCATCGGGCCTCAGGAAGGTACGTGTGGCACAGCTGCATTCCGCCGCGAGCTGGTGGTGACAGCGGATATAGCCCGGGATCCGAGCTGGGAGCGTTTTCGCAGTCTGGCGCTAGGGTACAATCTACGCTCCTGCTGGTCGTTGcc                                                                                                                                                    >  2:572660/1‑150 (MQ=255)
 ttGCCCATCGGGCCTCAGGAAGGTACGTGTGGCACAGCTGCATTCCGCCGCGAGCTGGTGGTGACAGCGGATATAGCCCGGGATCCGAGCTGGGAGCGTTTTCGCAGTCTGGCGCTAGGGTACAATCTACGCTCCTGCTGGTCGTTGCCa                                                                                                                                                   <  2:769228/150‑1 (MQ=255)
         cGGGCCTCAGGAAGGTACGTGTGGCACAGCTGCATTCCGCCGCGAGCTGGTGGTGACAGCGGATATAGCCCGGGATCCGAGCTGGGAGCGTTTTCGCAGTCTGGCGCTAGGGTACAATCTACGCTCCTGCTGGTCGTTGCCATTACtctc                                                                                                                                           <  1:572660/150‑1 (MQ=255)
         cGGGCCTCAGGAAGGTACGTGTGGCACAGCTGCATTCCGACGCGCGCTGGTTGTGACAGCGGATATAGCCCGGGGTTCGAGCTGGGAGCGTTTTCGCAGTCTGGCGCTAGGGTACAATCTACGCTCCTGCTGGTCGTTGCCATTACtctc                                                                                                                                           <  1:248863/150‑1 (MQ=255)
           ggCCTCAGGAAGGTACGTGTGGCACAGCTGCATTCCGCCGCGAGCTGGTGGTGACAGCGGATATAGCCCGGGATCCGAGCTGGGAGCGTTTTCGCAGTCTGGCGCTAGGGTACAATCTACGCTCCTGCTGGTCGTTGCCATTACTCTCGc                                                                                                                                         <  2:338184/150‑1 (MQ=255)
              cTCAGGAAGGTACGTGTGGCACAGCTGCATTCCGCCGCGAGCTGGTGGTGACAGCGGATATAGCCCGGGATCCGAGCTGGGAGCGTTTTCGCAGTCTGGCGCTAGGGTACAATCTACGCTCCTGCTGGTCGTTGCCATTACTCTCGCATg                                                                                                                                      >  1:843658/1‑150 (MQ=255)
                cAGGAAGGTACGTGTGGCACAGCTGCGTTCCGCCGCGAGCTGGTGGTGACAGCGGATATAGCCCGGGATCCGAGCTGGGAGCGTTTTCGCAGTCTGGCGCTAGGGTACAATCTACGCTCCTGCTGGTCGTTGCCATTACTCTCGCATGAg                                                                                                                                    <  2:323043/150‑1 (MQ=255)
                 aGGAAGGTACGTGTGGCACAGCTGCATTCCGCCGCGAGCTGGTGGTGACAGCGGATATAGCCCGGGATCCGAGCTGGGAGCGTTTTCGCAGTCTGGCGCTAGGGTACAATCTACGCTCCTGCTGGTCGTTGCCATTACTCTCGCATGAgg                                                                                                                                   >  1:428790/1‑150 (MQ=255)
                 aGGAAGGTACGTGTGGCACAGCTGCATTCCGCCGCGAGCTGGTGGTGACAGCGGATATAGCCCGGGATCCGAGCTGGGAGCGTTTTCGCAGTCTGGCGCTAGGGAACAATCTACGCTCCTGCTGGTCGTTGCCATTACTCTCGCATGAgg                                                                                                                                   >  1:341249/1‑150 (MQ=255)
                   gAAGGTACGTGTGGCACAGCTGCATTCCGCCGCGAGCTGGTGGTGACAGCGGATATAGCCCGGGATCCGAGCTGGGAGCGTTTTCGCAGTCTGGCGCTAGGGTACAATCTACGCTCCTGCTGGTCGTTGCCATTACTCTCGCATGAGGGc                                                                                                                                 >  1:844221/1‑150 (MQ=255)
                   gAAGGTACGTGTGGCACAGCTGCATTCCGCCGCGAGCTGGTGGTGACAGCGGATATAGCCCGGGATCCGAGCTGGGAGCGTTTTCGCAGTCTGGCGCTAGGGTACAATCTACGCTCCTGCTGGTCGTTACCATTACTCTCGCATGAGGGc                                                                                                                                 >  1:360424/1‑150 (MQ=255)
                   gAAGGTACGTGTGGCACAGCTGCATTCCGCCGCGAGCTGGTGGTGACAGCGGATATAGCCCGGGATCCGAGCTGGGAGCGTTTTCGCAGTCTGGCGCTAGGGTACAATCTACGCTCCTGCTGGTCGTTACCATTACTCTCGCATGAGGGc                                                                                                                                 >  1:360547/1‑150 (MQ=255)
                       gTACGTGTGGCACAGCTGCATTCCGCCGCGAGCTGGTGGTGACAGCGGATATAGCCCGGGATCCGAGCTGGGAGCGTTTTCGCAGTCTGGCGCTAGGGTACAATCTACGCTCCTGCTGGTCGTTGCCATTACTCTCGCATGAGGGCTCCg                                                                                                                             >  1:342922/1‑150 (MQ=255)
                       gTACGTGTGGCACAGCTGCATTCCGCCGCGAGCTGGTGGTGACAGCGGATATAGCCCGGGATCCGAGCTGGGAGCGTTTTCGCAGTCTGGCGCTAGGGTACAATCTACGCTCCTGCTGGTCGTTGCCATTACTCTCGCATGAGGGCTCCg                                                                                                                             >  2:458126/1‑150 (MQ=255)
                             gtgGCACAGCTGCATTCCGCCGCGAGCTGGTGGTGACAGCGGATATAGCCCGGGATCCGAGCTGGGAGCGTTTTCGCAGTCTGGCGCTAGGGTACAATCTACGCTCCTGCTGGTCGTTGCCATTACTCTCGCATGAGGGCTCCGTTCTTg                                                                                                                       <  2:434997/150‑1 (MQ=255)
                             gtgGCACAGCTGCATTCCGCCGCGAGCTGGTGGTGACAGCGGATATAGCCCGGGATCCGAGCTGGGAGCGTTTTCGCAGTCTGGCGCTAGGGTACAATCTACGCTCCTGCTGGTCGTTGCCATTACTCTCGCATGAGGGCTCCGTTCTTg                                                                                                                       <  2:1080905/150‑1 (MQ=255)
                                       tGCATTCCGCCGCGAGCTGGTGGTGACAGCGGATATAGCCCGGGATCCGAGCTGGGAGCGTTTTCGCAGTCTGGCGCTAGGGTACAATCTACGCTCCTGCTGGTCGTTGCCATTACTCTCGCATGAGGGCTCCGTTCTTGGGACATTTgc                                                                                                             <  2:843658/150‑1 (MQ=255)
                                                    gAGCTGGTGGTGACAGCGGATATAGCCCGGGATCCGAGCTGGGAGCGTTTTCGCAGTCTGGCGCTAGGGTACAATCTACGCTCCTGCTGGTCGTTGCCATTACTCTCGCATGAGGGCTCCGTTCTTGGGACATTTGCGCTATACCAGGAt                                                                                                >  2:598774/1‑150 (MQ=255)
                                                       cTGGTGGTGACAGCGGATATAGCCCGGGATCCGAGCTGGGAGCGTTTTCGCAGTCTGGCGCTAGGGTACAATCTACGCTCCTGCTGGTCGTTGCCATTACTCTCGCATGAGGGCTCCGTTCTTGGGACATTTGCGCTATACCAGGATCaa                                                                                             <  1:507797/150‑1 (MQ=255)
                                                            ggtgACAGCGGATATAGCCCGGGATCCGAGCTGGGAGCGTTTTCGCAGTCTGGCGCTAGGGTACAATCTACGCTCCTGCTGGTCGTTGCCATTACTCTCGCATGAGGGCTCCGTTCTTGGGACATTTGCGCTATACCAGGATCAAGCGAt                                                                                        <  1:628200/150‑1 (MQ=255)
                                                              tgACAGCGGATATAGCCCGGGATCCGAGCTGGGAGCGTTTTCGCAGTCTGGCGCTAGGGTACAATCTACGCTCCTGCTGGTCGTTGCCATTACTCTCGCATGAGGGCTCCGTTCTTGGGACATTTGCGCTATACCAGGATCAAGCGATTg                                                                                      >  2:778455/1‑150 (MQ=255)
                                                                  aGCGGATATAGCCCGGGATCCGAGCTGGGAGCGTTTTCGCAGTCTGGCGCTAGGGTACAATCTACGCTCCTGCTGGTCGTTGCCATTACTCTCGCATGAGGGCTCCGTTCTTGGGACATTTGCGCTATACCAGGATCAAGCGATTGCGcc                                                                                  <  1:458126/150‑1 (MQ=255)
                                                                       atatAGCCCGGGATCCGAGCTGGGAGCGTTTTCGCAGTCTGGCGCTAGGGTACAATCTACGCTCCTGCTGGTCGTTGCCGTTACTCTCGCATGAGGGCTCCGTTCTTGGGACATTTGCGCTATACCAGGATCAAGCGCTTGCGCCAACCg                                                                             >  1:156937/1‑150 (MQ=255)
                                                                           aGCCCGGGATCCGAGCTGGGAGCGTTTTCGCAGTCTGGCGCTAGGGTACAATCTACGCTCCTGCTGGTCGTTGCCATTACTCTCGCATGAGGGCTCCGTTCTTGGGACATTTGCGCTATACCAGGATCAAGCGATTGCGCCAAGCGAGGt                                                                         <  2:844221/150‑1 (MQ=255)
                                                                             cccGGGATCCGAGCTGGGAGCGTTTTCGCAGTCTGGCGCTAGGGTACAATCTACGCTCCTGCTGGTCGTTGCCATTACTCTCGCATGAGGGCTCCGTTCTTGGGACATTTGCGCTATACCAGGATCAAGCGATTGCGCCAAGCGAGGTGc                                                                       >  1:398461/1‑150 (MQ=255)
                                                                                gggATCCGAGCTGGGAGCGTTTTCGCAGTCTGGCGCTAGGGTACACTGTACGCTCCTGCTGGTCGTTGCCATTACTCTCGCATGAGGGCTCCGTTCTTGGGACATTTGCGCTATACCAGGATCAAGCGATTGCGCCAAGCGAGGTGCAGa                                                                    <  2:422569/150‑1 (MQ=255)
                                                                                      cGAGCTGGGAGCGTTTTCGCAGTCTGGCGCTAGGGTACAATCTACGCTCCTGCTGGTCGTTGCCATTACTCTCGCATGAGGGCTCCGTTCTTGGGACATTTGCGCTATACCAGGATCAAGCGATTGCGCCAAGCGAGGTGCAGATCCATc                                                              <  1:598774/150‑1 (MQ=255)
                                                                                       gAGCTGGGAGCGTTTTCGCAGTCTGGCGCTAGGGTACAATCTACGCTCCTGCTGGTCGTTGCCATTACTCTCGCATGAGGGCTCCGTTCTTGGGGCATTTGCGCTATACCAGGATCAAGCGATTGCGCCAAGCGAGGTGCAGAtagatcg                                                             >  1:242955/1‑144 (MQ=255)
                                                                                       gAGCTGGGAGCGTTTTCGCAGTCTGGCGCTAGGGTACAATCTACGCTCCTGCTGGTCGTTGCCATTACTCTCGCATGAGGGCTCCGTTCTTGGGACATTTGCGCTATACCAGGATCAAGCGATTGCGCCAAGCGAGGTGCAGATCCATCg                                                             >  1:483775/1‑150 (MQ=255)
                                                                                             ggAGCGTTTTCGCAGTCTGGCGCTAGGGTACAATCTACGCTCCTGCTGGTCGTTGCCATTACTCTCGCATGAGGGCTCCGTTCTTGGGACATTTGCGCTATACCAg                                                                                                   >  1:847216/1‑106 (MQ=255)
                                                                                             ggAGCGTTTTCGCAGTCTGGCGCTAGGGTACAATCTACGCTCCTGCTGGTCGTTGCCATTACTCTCGCATGAGGGCTCCGTTCTTGGGACATTTGCGCTATACCAg                                                                                                   <  2:847216/106‑1 (MQ=255)
                                                                                             ggAGCGTTTTCGCAGTCTGGCGCTAGGGTACAATCTACGCTCCTGCTGGTCGTTGCCATTACTCTCGCATGAGGGCTCCGTTCTTGGGACATTTGCGCTATACCAGGATCAAGCGATTGCGCCAAGCGAGGTGCAGATCCATCGGCTGAc                                                       >  1:728420/1‑150 (MQ=255)
                                                                                                       cGCAGTCTGGCGCTAGGGTACAATCTACGCTCCTGCTGGTCGTTGCCATTACTCTCGCATGAGGGCTCCGTTCTTGGGACATTTGCGCTATACCAGGATCAAGCGATTGCGCCAAGCGAGGTGCAGATCCATCGGCTGACCTGCgcggcg                                             <  2:1037200/150‑1 (MQ=255)
                                                                                                       agaggTCTGGCGCGAGGGTACGATGTGCGCTTCTGCTGGTCGTTGGCAGTACGCTCGCATGAGGGCTCCCTTCTTGGGACAGGTGCGCTATCCCAGGATCAAGCGATTGCGCCAAGCGAGGTGCAGATGCATCGGCTGACCTGCgcggcg                                             <  2:341249/146‑1 (MQ=255)
                                                                                                          aGTCTGGCGCTAGGGTACAATCTACGCTCCTGCTGGTCGTTGCCATTACTCTCGCATGAGGGCTCCGTTCTTGGGACATTTGCGCTATACCAGGATCAAGCGATTGCGCCAAGCGAGGTGCAGATCCATCGGCTGACCTGCGCGGCGCaa                                          >  2:55586/1‑150 (MQ=255)
                                                                                                            tCTGGCGCTAGGGTACAATCTACGCTCCTGCTGGTCGTTGCCATTACTCTCGCATGAGGGCTCCGTTCTTGGGACATTTGCGCTATACCAGGATCAAGCGATTGCGCCAAGCGAGGTGCAGATCCATCGGCTGACCTGCGCGGCGCAACt                                        <  1:55586/150‑1 (MQ=255)
                                                                                                                        gTACAATCTACGCTCCTGCTGGTCGTTGCCATTACTCTCGCATGAGGGCTCCGTTCTTGGGACATTTGCGCTATACCAGGATCAAGCGATTGCGCCAAGCGAGGTGCAGATCCATCGGCTGACCTGCGCGGCGCAACTGGCTGc                                  >  2:96190/1‑144 (MQ=255)
                                                                                                                        gTACAATCTACGCTCCTGCTGGTCGTTGCCATTACTCTCGCATGAGGGCTCCGTTCTTGGGACATTTGCGCTATACCAGGATCAAGCGATTGCGCCAAGCGAGGTGCAGATCCATCGGCTGACCTGCGCGGCGCAACTGGCTGc                                  <  1:96190/144‑1 (MQ=255)
                                                                                                                                 aCGCTCCTGCTGGTCGTTGCCATTACTCTCGCATGAGGGCTCCGTTCTTGGGACATTTGCGCTATACCAGGATCAAGCGATTGCGCCAAGCGAGGTGCAGATCCATCGGCTGACCTGCGCGGCGCAACTGGCTGCCATTGCGATCCGGCa                   <  2:342922/150‑1 (MQ=255)
                                                                                                                                   gCTCCTGCTGGTCGTTGCCATTACTCTCGCATGAGGGCTCCGTTCTTGGGACATTTGCGCTATACCAGGATCAAGCGATTGCGCCAAGCGAGGTGCAGATCCATCGGCTGACCTGCGCGGCGCAACTGGCTGCCATTGCGATCCGGCATg                 >  1:972449/1‑150 (MQ=255)
                                                                                                                                   gCTCCTGCTGGTCGTTGCCATTACTCTCGCATGAGGGCTCCGTTCTTGGGACATTTGCGCTATACCAGGATCAAGCGATTGCGCCAAGCGAGGTGCAGATCCATCGGCTGACCTGCGCGGCGCAACTGGCTGCCATTGCGATCCGGCATg                 >  2:941315/1‑150 (MQ=255)
                                                                                                                                            ggTCGTTACCATTACTCTCGCATGAGGGCTCCGTTCTTGGGACATTTGCGCTATACCAGGATCAAGCGATTGCGCCAAGCGAGGTGCAGATCCATCGGCTGACCTGCGCGGCGCAACTGGCTGCCATTGCGATCCGGCATGAGCGTGACg        <  2:360547/150‑1 (MQ=255)
                                                                                                                                            ggTCGTTACCATTACTCTCGCATGAGGGCTCCGTTCTTGGGACATTTGCGCTATACCAGGATCAAGCGATTGCGCCAAGCGAGGTGCAGATCCATCGGCTGACCTGCGCGGCGCAACTGGCTGCCATTGCGATCCGGCATGAGCGTGACg        <  2:360424/150‑1 (MQ=255)
                                                                                                                                               cGTTGCCATTACTCTCGCATGAGGGCTCCGTTCTTGGGACATTTGCGCTATACCAGGATCAAGCGATTGCGCCAAGCGAGGTGCAGATCCATCGGCTGACCTGCGCGGCGCAACTGGCTGCCATTGCGATCCGGCATGAGCGTGACGGcc     <  2:972449/150‑1 (MQ=255)
                                                                                                                                                gTTGCCATTACTCTCGCATGAGGGCTCCGTTCTTGGGACATTTGCGCTATACCAGGATCAAGCGATTGCGCCAAGCGAGGTGCAGATCCATCGGCTGACCTGCGCGGCGCAACTGGCTGCCATTGCGATCCGGCATGAGCGTGACGGCCa    >  1:706561/1‑150 (MQ=255)
                                                                                                                                                 ttGCCATTACTCTCGCATGAGGGCTCCGTTCTTGGGACATTTGCGCTATACCAGGATCAAGCGATTGCGCCAAGCGAGGTGCAGATCCATCGGCTGACCTGCGCGGCGCAACTGGCTGCCATTGCGATCCGGCATGAGCGTGACGGCCaa   <  1:778455/150‑1 (MQ=255)
                                                                                                                                                  tGCCATTACTCTCGCATGAGGGCTCCGTTCTTGGGACATTTGCGCTATACCAGGATCAAGCGATTGCGCCAAGCGAGGTGCAGATCCATCGGCTGACCTGCGCGGCGCAACTGGCTGCCATTGCGATCCGGCATGAGCGTGACGGCCAAc  <  1:1067797/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CTTGCCCATCGGGCCTCAGGAAGGTACGTGTGGCACAGCTGCATTCCGCCGCGAGCTGGTGGTGACAGCGGATATAGCCCGGGATCCGAGCTGGGAGCGTTTTCGCAGTCTGGCGCTAGGGTACAATCTACGCTCCTGCTGGTCGTTGCCATTACTCTCGCATGAGGGCTCCGTTCTTGGGACATTTGCGCTATACCAGGATCAAGCGATTGCGCCAAGCGAGGTGCAGATCCATCGGCTGACCTGCGCGGCGCAACTGGCTGCCATTGCGATCCGGCATGAGCGTGACGGCCAAC  >  NC_002947/3754842‑3755137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑