| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,151,995 | G→A | 10.2% | intergenic (+141/+10) | PP_3655 → / ← PP_3656 | cytosine/purine/uracil/thiamine/allantoin permease family protein/aromatic compound‑specific porin |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,151,995 | 0 | G | A | 10.2% | 103.7 / 3.2 | 39 | intergenic (+141/+10) | PP_3655/PP_3656 | cytosine/purine/uracil/thiamine/allantoin permease family protein/aromatic compound‑specific porin |
| Reads supporting (aligned to +/- strand): ref base G (15/20); new base A (2/2); total (17/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01 | |||||||||||
CTTAATCGGTACTGGCCTTTCGTTGCAAAAGATGAAACGGCTGGGTGCAGGCGTCCGAACGCCTGGTGCAATTCAGGCGGAAAACAGCGAACCCTTCGATTGGGAATAAATGGCGAAAAATGGGCCGG‑CTTCAGAAGGCCGGCCCGTTTCGCTATTTAGAAGTTGAAGGTGTAGCGCATGATCAGGCGATTTTCATTAGCGCTGTCAGCGTAGGTCGAGCGGTTCGTGGAGTTACGCCACTGAAACGAAACACCCTTCGCCGGACCACTCTGAATAACATAAG > NC_002947/4151850‑4152132 | cTTAATCGGTACTGGCCTTTCGTTGCAAAAGATGAAACGGCTGGGTGCAGGCGTCCGAACGCCTGGTGCAATTCAGGCGGAAAACAGCGAACCCTTCGATTGGGAATAAATGGCGAAAAATGGGCCGG‑CTTCAGAAGGCCGGCCCGTTTc > 2:246251/1‑150 (MQ=255) ttAATCGGTACTGGCCTTTCGTTGCAAAAGATGAAACGGCTGGGTGCAGGCGTCCGAACGCCTGGTGCAATTCAGGCGGAAAACAGCGAACCCTTCGATTGGGAATAAATGGCGAAAAATGGGCCGG‑CTTCAGAAGGCCGGCCCGTTTCg < 2:648623/150‑1 (MQ=255) tAATCGGTACTGGCCTTTCGTTGCAAAAGATGAAACGGCTGGGTGCAGGCGTCCGAACGCCTGGTGCAATTCAGGCGGAAAACAGCGAACCCTTCGATTGGGAATAAATGGCGAAAAATGGGCCGG‑CTTCAGAAGGCCGGCCCGTTTCGc < 1:297356/150‑1 (MQ=255) aaTCGGTACTGGCCTTTCGTTGCAAAAGATGAAACGGCTGGGTGCAGGCGTCCGAACGCCTGGTGCAATTCAGGCGGAAAACAGCGAACCCTTCGATTGGGAATAAATGGCGAAAAATGGGCCGG‑CTTCAGAAGGCCGGCCCGTTTCGCt < 1:173873/150‑1 (MQ=255) tttCGTTGCAAAAGATGAAACGGCTGGGTGCAGGCGCCGTGTGGCCTGGTGCAATTCAGGCGGAAAACAGCGAACCCTTCGATTGGGAATAAATGGCGAAAAATGGGCCGG‑CTTCAGAAGGCCGGCCCGTTTCGCTATTTAGAAGTTGaa < 1:851596/150‑1 (MQ=255) ttCGTTGCAAAAGATGAAACGGCTGGGTGCAGGCGTCCGAACGCCTGGTGCAATTCAGGCGGAAAACAGCGAACCCTTCGATTGGGAATAAATGGCGAAAAATGGGCCGG‑CTTCAGAAGGCCGGCCCGTTTCGCTATTTAGAAGTTGAAg < 1:452949/150‑1 (MQ=255) ttCGTTGCAAAAGATGAAACGGCTGACGTCAGGCGTCCGAACGCCTGGTGCAATTCAGGCGGAAAACAGCGAACCCTTCGATTGGGAATAAATGGCGAAAAATGGGCCGG‑CTTCAGAAGGCCGGCCCGTTTCGCTATTTAGAAGTTGAAg < 1:550158/150‑1 (MQ=255) aaGATGAAACGGCTGGGTGCAGGCGTCCGAACGCCTGGTGCAATTCAGGCTGAATACAGCGTATTCTTTGATTGATAATAAATGGCGAAAAATGGGCCGG‑CTTCTTAAGGCCGGCCCGTTTCGCTATTTAGAAGTTGAAGGTGTAGCGCa < 1:570862/150‑1 (MQ=255) gATGAAACGGCTGGGTGCAGGCGTCCGAACGCCTGGTGCAATTCAGGCGGAAAACAGCGAACCCTTCGATTGGGAATAAATGGCGAAAAATGGGCCGGCCTTCTGAA‑GCCGGCCCAttt < 1:1063233/119‑1 (MQ=255) gATGAAACGGCTGGGTGCAGGCGTCCGAACGCCTGGTGCAATTCAGGCGGAAAACAGCGAACCCTTCGATTGGGAATAAATGGCGAAAAATGGGCCGGCCTTCTGAA‑GCCGGCCCAttt > 2:1063233/1‑119 (MQ=255) gATGAAACGGCTGGGTGCAGGCGTCCGAACGCCTGGTGCAATTCAGGCGGAAAACAGCGAACCCTTCGATTGGGAATAAATGGCGAAAAATGGGCCGG‑CTTCAGAAGGCCGGCCCGTTTCGCTATTTAGAAGTTGAAGGTGTAGCGCATg > 1:703151/1‑150 (MQ=255) gATGAAACGGCTGGGTGCAGGCGTCCGAACGCCTGGTGCAATTCAGGCGGAAAACAGCGAACCCTTCGATTGGGAATAAATGGCGAAAAATGGGCCGG‑CTTCAGAAGGCCGGCCCGTTTCGCTATTTAGAAGTTGAAGGTGTAGCGCATg > 1:950854/1‑150 (MQ=255) aTGAAACGGCTGGGTGCAGGCGTCCGAACGCCTGGTGCAATTCAGGCGGAAAACAGCGAACCCTTCGATTGGGAATAAATGGCGAAAAATGGGCCGG‑CTTCAGAAGGCCGGCCCGTTTCGCTATTTAGAAGTTGAAGGTGTAGCGCATGa < 2:426443/150‑1 (MQ=255) gCGTCCGAACGCCTGGTGCAATTCAGGCGGAAAACAGCGAACCCTTCGATTGGGAATAAATGGCGAAAAATGGGCCGG‑CTTCAGGAGGCCGGCCCGTTTCGCTATTTAGAAGTTGAAGGTGTAGCGCATGATCAGGCGATTTTCATTAgc > 2:102103/1‑150 (MQ=255) gAACGCCTGGTGCAATTCAGGCGGAAAACAGCGAACCCTTCGATTGGGAATAAATGGCGAAAAATGGGCCGG‑CTTCAGAAGGCCGGCCCGTTTCGCTATTTAGAAGTTGAAGGTGTAGCGCATGATCAGGCGATTTTCATTAGCGCNGTc < 2:703151/150‑1 (MQ=255) gAACGCCTGGTGCAATTCAGGCGGAAAACAGCGAACCCTTCGATTGGGAATAAATGGCGAAAAACGGGCCGGCCTTCTGAA‑GCCGGCCCAtttttc < 1:1057146/96‑4 (MQ=25) gAACGCCTGGTGCAATTCAGGCGGAAAACAGCGAACCCTTCGATTGGGAATAAATGGCGAAAAACGGGCCGGCCTTCTGAA‑GCCGGCCCAtttttc > 2:1057146/1‑93 (MQ=21) gCCTGGTGCAATTCAGGCGGAAAACAGCGAACCCTTCGATTGGGAATAAATGGCGAAAAATGGGCCGG‑CTTCAGAAGGCCGGCCCGTTTCGCTATTTAGAAGTTGAAGGTGTAGCGCATGATCAGGCGATTTTCATTAGCGCTGTCAGCg > 1:18098/1‑150 (MQ=255) cTGGTGCAATTCAGGCGGAAAACAGCGAACCCTTCGATTGGGAATAAATGGCGAAAAATGGGCCGG‑CTTCAGAAGGCCGGCCCGTTTCGCTATTTAGAAGTTGAAGGTGTAGCGCATGAAGTGGCGATTTTCATTAGCGCTGTCAGCGTa < 1:203209/150‑1 (MQ=255) ggTGCAATTCAGGCGGAAAACAGCGAACCCTTCGATTGGGAATAAATGGCGAAAAATGGGCCGG‑CTTCAGAAGGCCGGCCCGTTTCGCTATTTAGAAGTTGAAGGTGTAGCGCATGATCAGGCGATTTTCATTAGCGCTGTCa > 1:152029/1‑143 (MQ=255) ggTGCAATTCAGGCGGAAAACAGCGAACCCTTCGATTGGGAATAAATGGCGAAAAATGGGCCGG‑CTTCAGAAGGCCGGCCCGTTTCGCTATTTAGAAGTTGAAGGTGTAGCGCATGATCAGGCGATTTTCATTAGCGCTGTCa < 2:152029/143‑1 (MQ=255) aGGCGGAAAACAGCGAACCCTTCGATTGGGAATAAATGGCGAAAAATGGGCCGG‑CTTCAGAAGGCCGGCCCGTTTCGCTATTTAGAAGTTGAAGGTGTAGCGCa < 1:194072/104‑1 (MQ=255) aGGCGGAAAACAGCGAACCCTTCGATTGGGAATAAATGGCGAAAAATGGGCCGG‑CTTCAGAAGGCCGGCCCGTTTCGCTATTTAGAAGTTGAAGGTGTAGCGCa > 2:194072/1‑104 (MQ=255) aGGCGGAAAACAGCGAACCCTTCGATTGGGAATAAATGGCGAAAAATGGGCCGG‑CTTCAGAAGGCCGGCCCGTTTCGCTATTTAGAAGTTGAAGGTGTAGCGCATGATCAGGCGATTTTCATTAGCGCTGTCAGCGTAGGTCGAGCGGtt > 2:464715/1‑150 (MQ=255) ggAAAACAGCGAACCCTTCGATTGGGAATAAATGGCGAAAAATGGGCCGG‑CTTCAGAAGGCCGGCCCGTTTCGCTATTTAGAAGTTGAAGGTGTAGCGCATGATCAGGCGATTTTCATTAGCGCTGTCAGCGTAGGTCGAGCGGTTCGTg > 2:995257/1‑150 (MQ=255) aaaCAGCGAACCCTTCGATTGGGAATAAATGGCGAAAAATGGGCCGG‑CTTCAGAAGGCCGGCCCGTTTCGCTATTTAGAAGTTGAAGGTGTAGCGCATGATCAGGCGATTTTCATTAGCGCTGTCAGCGTAGGTCGAGCGGTTCGTGGAg < 2:834593/150‑1 (MQ=255) aGCGAACCCTTCGATTGGAAATAAATGGCGAAAAATGGGCCGG‑CTTCAGAAGGCCGGCCCGTTTCGCTATTTAGAAGTTGAAGGTGTAGCGCATGATCAGGCGATTTTCATTAGCGCTGTCAGCGTAGGTCGAGCGGTTCGTGGAGTTAc < 1:478701/150‑1 (MQ=255) cTTCGATTGGGAATAAATGGCGAAAAATGGGCCGG‑CTTCAGAAGGCCGGCCCGTTTCGCTATTTAGAAGTTGAAGGTGTAGCGCATGATCAGGCGATTTTCATTAgc > 1:575672/1‑107 (MQ=255) cTTCGATTGGGAATAAATGGCGAAAAATGGGCCGG‑CTTCAGAAGGCCGGCCCGTTTCGCTATTTAGAAGTTGAAGGTGTAGCGCATGATCAGGCGATTTTCATTAgc < 2:575672/107‑1 (MQ=255) ttCGATTGGGAATAAATGGCGAAAAATGGGCCGG‑CTTCAGAAGGCCGGCCCGTTTCGCTATTTAGAAGTTGAAGGTGTAGCGCATGATCAGGGCATTTTCATTAGCGCTGTCAGCGGAGGTCGAGCGGTTCGTGGAGTTACGCCACTgaa > 1:852191/1‑150 (MQ=255) ttCGATTGGGAATAAATGGCGAAAAATGGGCCGG‑CTTCAGAAGGCCGGCCCGTTTCGCTATTTAGAAGTTGAAGGTGTAGCGCATGATCAGGCGATTTTCATTAGCGCTGTCAGCGTAGGTCGAGCGGTTCGTGGAGTTACGCCACTgaa > 2:681476/1‑150 (MQ=255) tCGATTGGGAATAAATGGCGAAAAATGGGCCGG‑CTTCAGAAGGCCGGCCCGTTTCGCTATTTAGAAGTTGAAGGTGTAGCGCATGATCAGGCGATTTTCATTAGCGCTGTCAGCGTAGGTCGAGCGGTTCGTGGAGTTACGCCACTgaaa < 2:1047914/150‑1 (MQ=255) tGGGAATAAATGGCGAAAAATGGGCCGG‑CTTCAGAAGGCCGGCCCGTTTCGCTATTTAGAAGTTGAAGGTGTAGCGCATGATCAGGCGATTTTCATTAGCGCTGTCAGCGTAGGTCGAGCGGTTCGTGGAGTTACGCCACTgaaacgaaa < 1:246251/150‑1 (MQ=255) cGG‑CTTCAGAAGGCCGGCCCGTTTCGCTATTTAGAAGTTGAAGGTGTAGCGCATGATCAGGCGATTTTCATTAGCGCTGTCAGCGTAGGTCGAGCGGTTCGTGGa > 2:1125390/1‑105 (MQ=255) cGG‑CTTCAGAAGGCCGGCCCGTTTCGCTATTTAGAAGTTGAAGGTGTAGCGCATGATCAGGCGATTTTCATTAGCGCTGTCAGCGTAGGTCGAGCGGTTCGTGGa < 1:1125390/105‑1 (MQ=255) g‑CTTCAGAAGGCCGGCCCGTTTCGCTATTTAGAAGTTGAAGGTGTAGCGCATGATCAGGCGATTTTCATTAGCGCTGTCAGCGTAGGTCGAGCGGTTCGTGGAGTTACGCCACTGAAACGAAACACCCTTCGCCGGACCACTCTGAATaa > 2:488673/1‑150 (MQ=255) gAAGGCCGGCCCGTTTCGCTATTTAGAAGTTGAAGGTGTAGCGCATGATCAGGCGATTTTCATTAGCGCTGTCAGCGTAGGTCGAGCGGTTCGTGGAGTTACGCCACTGAAACGAAACACCCTTCGCCGGACCACTCTGAATAACATAag < 1:115269/150‑1 (MQ=255) cccGTTTCGCTATTTAGAAGTTGAAGGTGTAGCGCATGATCAGGCGATTTTCATTAGCGCTGTCAGCGTAGGTCGAGCGGTTc > 1:527006/1‑83 (MQ=255) cccGTTTCGCTATTTAGAAGTTGAAGGTGTAGCGCATGATCAGGCGATTTTCATTAGCGCTGTCAGCGTAGGTCGAGCGGTTc < 2:527006/83‑1 (MQ=255) | CTTAATCGGTACTGGCCTTTCGTTGCAAAAGATGAAACGGCTGGGTGCAGGCGTCCGAACGCCTGGTGCAATTCAGGCGGAAAACAGCGAACCCTTCGATTGGGAATAAATGGCGAAAAATGGGCCGG‑CTTCAGAAGGCCGGCCCGTTTCGCTATTTAGAAGTTGAAGGTGTAGCGCATGATCAGGCGATTTTCATTAGCGCTGTCAGCGTAGGTCGAGCGGTTCGTGGAGTTACGCCACTGAAACGAAACACCCTTCGCCGGACCACTCTGAATAACATAAG > NC_002947/4151850‑4152132 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |