Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,163,542 T→C 13.6% intergenic (‑37/‑145) pssA ← / → PP_3665 CDP‑diacylglycerol‑‑serine O‑phosphatidyltransferase/AraC family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,163,5420TC13.6% 102.1 / 8.0 44intergenic (‑37/‑145)pssA/PP_3665CDP‑diacylglycerol‑‑serine O‑phosphatidyltransferase/AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (17/21);  new base C (4/2);  total (21/23)
Fisher's exact test for biased strand distribution p-value = 4.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

GCTCGAACAGGCAGGCGCGGTAAGCCCTGGCATCGGGCAGCACCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGAGGCTCTGCTTTCTCACTGCCCCTGACCAACCAGGCCTCAGCTTGCACAGGAACAGCGCAATG  >  NC_002947/4163400‑4163689
                                                                                                                                              |                                                                                                                                                   
gcTCGAACAGGCAGGCGCGGTAAGCCCTGGCATCGGGCAGCACCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCtgcctgcc                                                                                                                                              >  1:433853/1‑150 (MQ=255)
gcTCGAACAGGCAGGCGCGGTAAGCCCTGGCATCGGGCAGCACCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCtgcctgcc                                                                                                                                              >  1:451888/1‑150 (MQ=255)
gcTCGAACAGGCAGGCGCGGTAAGCCCTGGCATCGGGCAGCACCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTAGGGGTTAACTTACGCTCACCTTCTCTGTGCAATCACTGTCGGCcccgccggca                                                                                                                                              >  2:29394/1‑146 (MQ=255)
    gAACAGGCAGGCGCGGTAAGCCCTGGCATCGGGCAGCACCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCcggcg                                                                                                                                          >  1:238973/1‑150 (MQ=255)
     aaCAGGCAGGCGCGGTAAGCCCTGGCATCGGGCAGCACCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCcggcgg                                                                                                                                         <  1:294749/150‑1 (MQ=255)
     aaCAGGCAGGCGCGGTAAGCCCTGGCATCGGGCAGCACCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCcggcgg                                                                                                                                         <  1:871843/150‑1 (MQ=255)
        aggcaggcGCGGTAAGCCCTGGCATCGGGCAGCACCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGc                                                                                                                                      <  2:27368/150‑1 (MQ=255)
        aggcaggcGCGGTAAGCCCTGGCATCGGGCAGCACCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGc                                                                                                                                      <  2:776167/150‑1 (MQ=255)
         ggcaggcGCGGTAAGCCCTGGCATCGGGCAGCACCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACGGTCGGCCCCGCCGGCAGGCAGGGcc                                                                                                                                     <  1:29394/150‑1 (MQ=255)
           caggcGCGGTAAGCCCTGGCATCGGGCAGCACCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCAt                                                                                                                                   <  1:969812/150‑1 (MQ=255)
            aggcGCGGTAAGCCCTGGCATCGGGCAGCACCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGACCTGCCTGCCGGCGGGGCCAtt                                                                                                                                  >  2:557605/1‑150 (MQ=255)
              gcgcGGTAAGCCCTGGCATCGGGCAGCACCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTcc                                                                                                                                >  2:973261/1‑150 (MQ=255)
               cgcgGTAAGCCCTGGCATCGGGCAGCACCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCt                                                                                                                               <  2:60070/150‑1 (MQ=255)
                gcgGTAAGCCCTGGCATCGGGCAGCACCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTg                                                                                                                              <  1:992448/150‑1 (MQ=255)
                         ccTGGCATCGGGCAGCACCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCCGCCGGCaggcagg                                                                                                                                        >  1:50184/1‑128 (MQ=255)
                              cATCGGGCAGCACCTGAATCGCTTCGTGTTCCATGGCGTAGCCTGGCAAGCCCGCCCGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCgg                                                                                                                <  2:238973/150‑1 (MQ=255)
                                       gcaCCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGAACGCGCGCTAGGATTGGTTCGGGGTTCACTGACGCGCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCGGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGa                                                                                                       >  1:861793/1‑150 (MQ=255)
                                           cTGGACCGTTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGCCGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGa                                                                                                   <  2:825190/150‑1 (MQ=255)
                                                    ttCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCCGCCGGCAGGCAGGGccgaca                                                                                                                                 <  1:322666/111‑5 (MQ=255)
                                                    ttCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCCGCCGGCAGGCAGGGccgaca                                                                                                                                 >  2:322666/1‑107 (MQ=255)
                                                         gggNCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCACCACTGGTGGATGATCGACCCGGCAACGAccc                                                                                     >  2:694230/1‑150 (MQ=255)
                                                             cATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCggg                                                                                                                                        <  1:711477/95‑1 (MQ=255)
                                                             cATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCggg                                                                                                                                        >  2:711477/1‑95 (MQ=255)
                                                              aTGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATaac                                                                                >  2:1115361/1‑150 (MQ=255)
                                                                 gCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATaacaac                                                                             <  1:197702/150‑1 (MQ=255)
                                                                           gCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCAcg                                                                   >  1:740629/1‑150 (MQ=255)
                                                                              aGCCCGCCAGCATGGTTTCGGTGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCCCTGCATCCGGGGTGTCCCATCTTCCCGGCAACGACCCATAACAACAGGGACCACGCGa                                                                <  2:451888/150‑1 (MQ=255)
                                                                              aGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGa                                                                >  2:1049007/1‑150 (MQ=255)
                                                                                 ccGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGAGGc                                                             >  1:675178/1‑150 (MQ=255)
                                                                                 ccGCCAGCAGGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGAGGc                                                             <  1:187285/150‑1 (MQ=255)
                                                                                         aTGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGAGGCTCTGCttt                                                     >  1:106219/1‑150 (MQ=255)
                                                                                          tGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGAGGCTCTGCTTtc                                                    >  1:823139/1‑150 (MQ=255)
                                                                                           ggTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGTCCCATAACAACAAGAACCACGCGAGGCTCTGCTTtct                                                   <  1:694230/150‑1 (MQ=255)
                                                                                             tttCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGGGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGAGGCTCTGCTTTCTCa                                                 <  2:433853/150‑1 (MQ=255)
                                                                                                   ggTTTACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGAGGCTCTGCTTTCTCACTGccc                                           <  2:552434/150‑1 (MQ=255)
                                                                                                       cACTGACGCTCACCTGCTCTGTGCAATCACTGTCGGCCATGCCTGCCGGGGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGAGGCTCTGCTTTCTCACTGCCCCTGa                                       <  2:909614/150‑1 (MQ=255)
                                                                                                                 cACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGAGGCTCTGCTTTCTCACTGCCCCTGACCAACCAGGc                             <  2:823139/150‑1 (MQ=255)
                                                                                                                  aCCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGAGGCTCTGCTTTCTCACTGCCACTGACCAACCAGGcc                            >  2:912650/1‑150 (MQ=255)
                                                                                                                          tgtgCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGAGGCTCTGCt                                                       >  2:919788/1‑115 (MQ=255)
                                                                                                                          tgtgCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGAGGCTCTGCt                                                       <  1:919788/115‑1 (MQ=255)
                                                                                                                               aaTCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGAGGCTCTGCTTTCTCACTGCCCCTGACCAACCAGGCCTCAGCTTGCACAg               >  1:104918/1‑150 (MQ=255)
                                                                                                                                aTCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTAATGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGAGGCTCTGCTTTCTCACTGCCCCTGACCAACCAGGCCTCAGCTTGCACAgg              >  1:262074/1‑150 (MQ=255)
                                                                                                                                  cACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGGTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGAGGCTCTGCTTTCTCACTGCCCCTGACCAACCAGGCCTCAGCTTGCACAGGaa            <  2:1079062/150‑1 (MQ=255)
                                                                                                                                   aggaatggCCCCGCCGGCAGGCAGGGCCATTCCTGGCCGCTGCATCCGGGGGGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGAGGCTCTGCTTTCTCACTGCCCCTGACCAACCAGGCCTCAGCTTGCACAGGAAc           >  2:720541/7‑150 (MQ=255)
                                                                                                                                    cTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGAGGCTCTGCTTTCTCACTGCCCCTGACCAACCAGGCCTCAGCTTGCACAGGAACa          <  1:1103449/150‑1 (MQ=255)
                                                                                                                                    cTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGAGGCTCTGCTTTCTCACTGCCCCTGACCAACCAGGCCTCAGCTTGCACAGGAACa          >  2:907855/1‑150 (MQ=255)
                                                                                                                                          gCCCCGCCGGCAGGCAGTGCCATTCCTGGCCGCTGCATCCGGGGGGTATGATCGACCCGGCAACGACCCATAACAACAACAACCACGCGAGGCTCTGCTTTCTCACTGCCCCTGACCAACCAGGCCTCAGCTTGCACAGGAACAGCGCaa    >  2:235649/1‑150 (MQ=255)
                                                                                                                                            cctgcctgCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGAGGCTCTGCTTTCTCACTGCCCCTGACCAACCAGGCCTCAGCTTGCACAGGAACAGCGCAATg  >  2:792468/1‑150 (MQ=255)
                                                                                                                                            cctgcctgCCGGCGGGGCCATTCCTCGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGAGGCTCTGCTTTCTCACTGCCCCTGACCAACCAGGCCTCAGCTTGCACAGGAACAGCGCAATg  <  1:973261/150‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                   
GCTCGAACAGGCAGGCGCGGTAAGCCCTGGCATCGGGCAGCACCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGAGGCTCTGCTTTCTCACTGCCCCTGACCAACCAGGCCTCAGCTTGCACAGGAACAGCGCAATG  >  NC_002947/4163400‑4163689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑