Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,415,076 T→C 9.3% intergenic (+141/+27) PP_5644 → / ← PP_3899 membrane protein/hicB family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,415,0760TC 9.3% 112.7 / 3.2 43intergenic (+141/+27)PP_5644/PP_3899membrane protein/hicB family protein
Reads supporting (aligned to +/- strand):  ref base T (21/18);  new base C (2/2);  total (23/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.47e-01

TAGATGGCTATGAAGCACTCCTCTTGAAGCCGCCATCAAGTGATCCCACAGTCCGCCTCGGCGGGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGAAATACTTTGCTTGGTGGCTGCCACGCTGGCCGCTAGGTGCAGGTCGTGCCCTACCCTCACATTGA  >  NC_002947/4414933‑4415224
                                                                                                                                               |                                                                                                                                                     
tAGATGGCTATGAAGCACTCCTCTTGAAGCCGCCATCAAGTGATCCCACAGTCCGCCTCGGCGGGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGt                                                                                                                                                 <  2:363241/150‑1 (MQ=255)
    tGGCTATGAAGCACTCCTCTTGAAGCCGCCATCAAGTGATCCCACAGTCCGCCTCGGCGGGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGc                                                                                                                                                    <  2:953729/143‑1 (MQ=255)
    tGGCTATGAAGCACTCCTCTTGAAGCCGCCATCAAGTGATCCCACAGTCCGCCTCGGCGGGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGc                                                                                                                                                    >  1:953729/1‑143 (MQ=255)
    tGGCTATGAAGCACTCCTCTTGAAGCCGCCATCAAGTGATCCCACAGTCCGCCTCGGCGGGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCgg                                                                                                                                             <  1:1085121/150‑1 (MQ=255)
    tGGCTATGAAGCACTCCTCTTGAAGCCGCCATCAAGTGATCCCACAGTCCGCCTCGGCGGGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCgg                                                                                                                                             >  1:146772/1‑150 (MQ=255)
               cACTCCTCTTGAAGCCGCCATCAAGTGATCCCACAGTCCGCCTCGGCGGGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTg                                                                                                                                  <  2:627749/150‑1 (MQ=255)
                  tcctcTTGAAGCCGCCATCAAGTGATCCCACAGTCCGCCTCGGCGGGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGggc                                                                                                                               >  2:852964/1‑150 (MQ=255)
                                   tCAAGTGATCCCACAGTCCGCCTCGGCGGGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGAcc                                                                                                             >  2:953353/1‑150 (MQ=255)
                                       gTGATCCCACAGTCCGCCTCGGCGGGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAgg                                                                                                         <  2:327739/150‑1 (MQ=255)
                                            cccACAGTCCGCCTCGGCGGGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTc                                                                                                    <  1:852964/150‑1 (MQ=255)
                                                     cGCCTCGGCGGGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAgcgc                                                                                           >  1:1045661/1‑150 (MQ=255)
                                                     cGCCTCGGCGGGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAgcgc                                                                                           <  2:1045661/150‑1 (MQ=255)
                                                      gCCTCGGCGGGCTTCACCATGCATTCACGCTTTTTTTTTGCCTTCCCCTGCACAGTGACGGCTCATCCGCCCCCCTACGTTCGCCGGTATTGAAGTGCGGGCTTTGCGTTGGGCGG‑TCCGTCGTGATGACCCACGTGCTCCCCAAGCGCt                                                                                          >  2:235088/1‑150 (MQ=255)
                                                      gCCTCGGCGGGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCACTGCAATGCggg                                                                                                                                            <  2:999211/101‑1 (MQ=255)
                                                      gCCTCGGCGGGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCACTGCAATGCggg                                                                                                                                            >  1:999211/1‑101 (MQ=255)
                                                          cggcggGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGc                                                                                      <  2:852529/150‑1 (MQ=255)
                                                            gcggGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGCgt                                                                                    <  1:975540/150‑1 (MQ=255)
                                                              gggCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGCgtgt                                                                                  >  2:216769/1‑150 (MQ=255)
                                                              gggCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGCgtgt                                                                                  >  1:103014/1‑150 (MQ=255)
                                                                 cTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGCGTGTTAg                                                                               >  1:517607/1‑150 (MQ=255)
                                                                       cATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGCGTGTCAGATCAtt                                                                         <  2:146772/150‑1 (MQ=255)
                                                                                      ttttCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTAcg‑c                                                                                                                      >  1:516080/1‑90 (MQ=255)
                                                                                      ttttCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTAcg‑c                                                                                                                      <  2:516080/90‑1 (MQ=255)
                                                                                             gCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCCACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGAAATACTTTGCTtggtg                                                   <  1:678824/150‑1 (MQ=255)
                                                                                               ccTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAg                                                                                                          >  1:656850/1‑93 (MQ=255)
                                                                                               ccTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAg                                                                                                          <  2:656850/93‑1 (MQ=255)
                                                                                                cTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGCGTGTCAGATCATTc                                                                        <  1:761222/126‑1 (MQ=255)
                                                                                                cTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGCGTGTCAGATCATTc                                                                        >  2:761222/1‑126 (MQ=255)
                                                                                                    ccTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCGTTCAGTGGTAGATCATTCAAGGAAATACTTTGCTTGGTGGCTGCCa                                            >  1:851401/1‑150 (MQ=255)
                                                                                                     cTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGAAATACTTTGCTTGGTGGCTGCCAc                                           >  1:708764/1‑150 (MQ=255)
                                                                                                           aGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGACGGCTACG‑CGGGAGGACCCAGGTACTCACTAAGCGCTTTGGGTGTCAGATCGTTCAAGGAAAGACTTTGCTTGGTGGCTGCCACGCTGGc                                     >  1:217083/1‑150 (MQ=255)
                                                                                                                                cTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGCgt                                                                                    <  1:494851/82‑1 (MQ=255)
                                                                                                                                cTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGCgt                                                                                    >  2:494851/1‑82 (MQ=255)
                                                                                                                                    gTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGAAATACTTTGCTTGGTGGCTGCCACGCTGGCCGCTAGGTGCAGGTCGTGCCCNAcc            <  2:696324/150‑1 (MQ=255)
                                                                                                                                       aGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGAAATACTTTGCTTGGTGGCTGCCACGCTGGCCGCTAGGTGCAGGTCGTGCCCTACCCTc         <  2:708764/150‑1 (MQ=255)
                                                                                                                                        gCCCGCACTGCAATGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGCgt                                                                                    <  2:72563/74‑1 (MQ=255)
                                                                                                                                        gCCCGCACTGCAATGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGCgt                                                                                    >  1:72563/1‑74 (MQ=39)
                                                                                                                                         gaagtaTTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGAAATACTTTGCTTGGTGACTGCAACGCTGGCAGCTAGGTGCAGGTCGTGCCGTACCCTcac       >  1:879536/6‑150 (MQ=255)
                                                                                                                                          ccGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGAAATACTTTGCTTGGTGGCTGCCACGCTGGCCGCTAGGTGCAGGTCGTGCCCTACCCTcaca      >  2:602693/1‑150 (MQ=255)
                                                                                                                                           cGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGAAATACTTTGCTTGGTGGCTGCCACGCTGGCCGCTAGGTGCAGGTCGTGCCCTACCCTCACAt     >  1:195557/1‑150 (MQ=255)
                                                                                                                                            gCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGAAATACTTTGCTTGGTGGCTGCCACGCTGGCCGCTAGGTGCAGGTCGTGCCCTACCCTCACAtt    >  2:461885/1‑150 (MQ=255)
                                                                                                                                            gCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGAAATACTTTGCTTGGTGGCTGCCACGCTGGCCGCTAGGTGCAGGTCGTGCCCTACCCTCACAtt    >  2:191051/1‑150 (MQ=255)
                                                                                                                                              aTTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGAAATACTTTGCTTGGTGGCTGCCACGCTGGCCGCTAGGTGCAGGTCGTGCCCTACCCTCACATTGa  <  1:312402/150‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                     
TAGATGGCTATGAAGCACTCCTCTTGAAGCCGCCATCAAGTGATCCCACAGTCCGCCTCGGCGGGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGAAATACTTTGCTTGGTGGCTGCCACGCTGGCCGCTAGGTGCAGGTCGTGCCCTACCCTCACATTGA  >  NC_002947/4414933‑4415224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑