| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,415,081 | G→A | 9.7% | intergenic (+146/+22) | PP_5644 → / ← PP_3899 | membrane protein/hicB family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,415,081 | 0 | G | A | 9.7% | 107.2 / 3.1 | 41 | intergenic (+146/+22) | PP_5644/PP_3899 | membrane protein/hicB family protein |
| Reads supporting (aligned to +/- strand): ref base G (20/17); new base A (2/2); total (22/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01 | |||||||||||
TAGATGGCTATGAAGCACTCCTCTTGAAGCCGCCATCAAGTGATCCCACAGTCCGCCTCGGCGGGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGAAATACTTTGCTTGGTGGCTGCCACGCTGGCCGCTAGGTGCAGGTCGTGCCCTACCCTCACATTGA > NC_002947/4414933‑4415224 | tAGATGGCTATGAAGCACTCCTCTTGAAGCCGCCATCAAGTGATCCCACAGTCCGCCTCGGCGGGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGt < 2:363241/150‑1 (MQ=255) tGGCTATGAAGCACTCCTCTTGAAGCCGCCATCAAGTGATCCCACAGTCCGCCTCGGCGGGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCgg < 1:1085121/150‑1 (MQ=255) tGGCTATGAAGCACTCCTCTTGAAGCCGCCATCAAGTGATCCCACAGTCCGCCTCGGCGGGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCgg > 1:146772/1‑150 (MQ=255) cACTCCTCTTGAAGCCGCCATCAAGTGATCCCACAGTCCGCCTCGGCGGGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTg < 2:627749/150‑1 (MQ=255) tcctcTTGAAGCCGCCATCAAGTGATCCCACAGTCCGCCTCGGCGGGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGggc > 2:852964/1‑150 (MQ=255) tCAAGTGATCCCACAGTCCGCCTCGGCGGGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGAcc > 2:953353/1‑150 (MQ=255) gTGATCCCACAGTCCGCCTCGGCGGGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAgg < 2:327739/150‑1 (MQ=255) cccACAGTCCGCCTCGGCGGGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTc < 1:852964/150‑1 (MQ=255) cGCCTCGGCGGGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAgcgc > 1:1045661/1‑150 (MQ=255) cGCCTCGGCGGGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAgcgc < 2:1045661/150‑1 (MQ=255) gCCTCGGCGGGCTTCACCATGCATTCACGCTTTTTTTTTGCCTTCCCCTGCACAGTGACGGCTCATCCGCCCCCCTACGTTCGCCGGTATTGAAGTGCGGGCTTTGCGTTGGGCGG‑TCCGTCGTGATGACCCACGTGCTCCCCAAGCGCt > 2:235088/1‑150 (MQ=255) gCCTCGGCGGGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCACTGCAATGCggg < 2:999211/101‑1 (MQ=255) gCCTCGGCGGGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCACTGCAATGCggg > 1:999211/1‑101 (MQ=255) cggcggGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGc < 2:852529/150‑1 (MQ=255) gcggGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGCgt < 1:975540/150‑1 (MQ=255) gggCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGCgtgt > 1:103014/1‑150 (MQ=255) gggCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGCgtgt > 2:216769/1‑150 (MQ=255) cTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGCGTGTTAg > 1:517607/1‑150 (MQ=255) cATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGCGTGTCAGATCAtt < 2:146772/150‑1 (MQ=255) ttttCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTAcg‑c < 2:516080/90‑1 (MQ=255) ttttCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTAcg‑c > 1:516080/1‑90 (MQ=255) gCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCCACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGAAATACTTTGCTtggtg < 1:678824/150‑1 (MQ=255) ccTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAg > 1:656850/1‑93 (MQ=255) ccTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAg < 2:656850/93‑1 (MQ=255) cTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGCGTGTCAGATCATTc > 2:761222/1‑126 (MQ=255) cTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGCGTGTCAGATCATTc < 1:761222/126‑1 (MQ=255) ccTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCGTTCAGTGGTAGATCATTCAAGGAAATACTTTGCTTGGTGGCTGCCa > 1:851401/1‑150 (MQ=255) cTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGAAATACTTTGCTTGGTGGCTGCCAc > 1:708764/1‑150 (MQ=255) aGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGACGGCTACG‑CGGGAGGACCCAGGTACTCACTAAGCGCTTTGGGTGTCAGATCGTTCAAGGAAAGACTTTGCTTGGTGGCTGCCACGCTGGc > 1:217083/1‑150 (MQ=255) cTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGCgt > 2:494851/1‑82 (MQ=255) cTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGCgt < 1:494851/82‑1 (MQ=255) gTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGAAATACTTTGCTTGGTGGCTGCCACGCTGGCCGCTAGGTGCAGGTCGTGCCCNAcc < 2:696324/150‑1 (MQ=255) aGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGAAATACTTTGCTTGGTGGCTGCCACGCTGGCCGCTAGGTGCAGGTCGTGCCCTACCCTc < 2:708764/150‑1 (MQ=255) gCCCGCACTGCAATGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGCgt < 2:72563/74‑1 (MQ=255) gCCCGCACTGCAATGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGCgt > 1:72563/1‑74 (MQ=39) gaagtaTTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGAAATACTTTGCTTGGTGACTGCAACGCTGGCAGCTAGGTGCAGGTCGTGCCGTACCCTcac > 1:879536/6‑150 (MQ=255) ccGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGAAATACTTTGCTTGGTGGCTGCCACGCTGGCCGCTAGGTGCAGGTCGTGCCCTACCCTcaca > 2:602693/1‑150 (MQ=255) cGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGAAATACTTTGCTTGGTGGCTGCCACGCTGGCCGCTAGGTGCAGGTCGTGCCCTACCCTCACAt > 1:195557/1‑150 (MQ=255) gCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGAAATACTTTGCTTGGTGGCTGCCACGCTGGCCGCTAGGTGCAGGTCGTGCCCTACCCTCACAtt > 2:191051/1‑150 (MQ=255) gCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGAAATACTTTGCTTGGTGGCTGCCACGCTGGCCGCTAGGTGCAGGTCGTGCCCTACCCTCACAtt > 2:461885/1‑150 (MQ=255) aTTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGAAATACTTTGCTTGGTGGCTGCCACGCTGGCCGCTAGGTGCAGGTCGTGCCCTACCCTCACATTGa < 1:312402/150‑1 (MQ=255) | TAGATGGCTATGAAGCACTCCTCTTGAAGCCGCCATCAAGTGATCCCACAGTCCGCCTCGGCGGGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACG‑CGTGATGACCCAGGTACTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGAAATACTTTGCTTGGTGGCTGCCACGCTGGCCGCTAGGTGCAGGTCGTGCCCTACCCTCACATTGA > NC_002947/4414933‑4415224 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |