| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,530,070 | T→A | 15.2% | intergenic (+32/‑136) | PP_4018 → / → topB | acetyltransferase/DNA topoisomerase III |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,530,070 | 0 | T | A | 15.2% | 72.5 / 8.2 | 33 | intergenic (+32/‑136) | PP_4018/topB | acetyltransferase/DNA topoisomerase III |
| Reads supporting (aligned to +/- strand): ref base T (14/14); new base A (3/2); total (17/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.88e-01 | |||||||||||
GGTGCACGCCACGCCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGCCATGC > NC_002947/4529930‑4530209 | ggTGCACGCCACGCCGTTCTATGAGCGGCTGGGCTTTCGGGTTGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACCGGCCTCTTCGCGGGTAAACCCGCGaa < 1:10950/150‑1 (MQ=255)ggTGCACGCCACGCCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGaa < 2:156841/150‑1 (MQ=255)ggTGCACGCCACGCCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTAAACCCGCGaa < 1:853208/150‑1 (MQ=255) cGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATc < 1:229970/150‑1 (MQ=255) ggCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTCCCCGCGAAGAGGCCGGCTCAGGCGATATACATcc < 2:541038/150‑1 (MQ=255) gCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATccc > 1:58478/1‑150 (MQ=255) tAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTGGTCATCCTGAGCGATGCTCGTGCTGGATCCATAACGTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAAc < 1:1098266/150‑1 (MQ=255) gTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCt < 2:58478/150‑1 (MQ=255) tCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTg < 2:526047/150‑1 (MQ=255) aTTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTAcatccatc > 1:949781/1‑150 (MQ=255) ttCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGATCAGGCGATATACATCCCCGCTGACAAAAAAc < 2:684282/136‑1 (MQ=255) ttCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGATCAGGCGATATACATCCCCGCTGACAAAAAAc > 1:684282/1‑136 (MQ=255) cTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGTGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACAtccatacaaa > 2:357/1‑148 (MQ=255) cTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGa > 1:929551/1‑150 (MQ=255) tCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCt < 1:777956/134‑1 (MQ=255) tCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCt > 2:777956/1‑134 (MQ=255) gCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTAcatccatc < 1:835328/136‑1 (MQ=255) gCATCCCGCAGGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTAcatccatc > 2:835328/1‑136 (MQ=255) cccGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGAGAAGAGGCCGGCTCAGCCCCTATACATCCCCGGTGTCAAAATACCTGTACATCCTTGCATGTAAAACTTGCctct < 2:12016/150‑1 (MQ=255) gTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTgcgc < 2:949781/150‑1 (MQ=255) aCTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTAcatcc > 2:356489/1‑106 (MQ=255) aCTCGCGCGCCTGATCCATCACCTGCACTGGCATCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTAcatcc < 1:356489/106‑1 (MQ=255) gcgcCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCcca > 2:1026244/1‑150 (MQ=255) ccTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTa > 2:244962/1‑140 (MQ=255) ccTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTa < 1:244962/140‑1 (MQ=255) gATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCAccc < 1:855392/150‑1 (MQ=255) tGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCg > 1:1036726/1‑150 (MQ=255) ggCCTCTTCGCGGGGAAACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCgg > 2:1108914/1‑150 (MQ=255) ctctTCGCGGGTTTACCCGCGAAGAGGCAGGCTCCGGCGATATACATCCCCGATGCCAGAAAACATTTCGCTATCGCCAGGTGAATCGGGCCGCGTCTCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGCCa > 2:679524/1‑150 (MQ=255) ctctTCGCGGGTAAACCCGCGAAGAGGCCAGTGCAGGTGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGccc > 2:1006529/1‑149 (MQ=38) ttCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCAACCCGATGAAGCGTCCCCGGCCATgc > 2:1074279/1‑150 (MQ=255) ttCGCGGGTTTACCCGCGAAGAGGCCGGCTAAAGCGATATACATCCACGCTGAAAAAAAAAATGTACAACCATCAAGATAAAACTTTCCACTGCTCTCCCGCTCGCGCTGCCTAGTGCCCACCACCCCGATGAAGCGTCCCCGGCCATgc > 2:125127/1‑150 (MQ=255) ttCGCGGGTAAACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGCCATgc > 2:419727/1‑150 (MQ=255) | GGTGCACGCCACGCCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGCCATGC > NC_002947/4529930‑4530209 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |