| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 35,009 | A→G | 19.3% | intergenic (+27/+31) | czrSA → / ← PP_0031 | metal‑sensor histidine kinase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 35,009 | 0 | A | G | 19.3% | 119.3 / 21.6 | 57 | intergenic (+27/+31) | czrSA/PP_0031 | metal‑sensor histidine kinase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (25/21); new base G (5/6); total (30/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.40e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.63e-01 | |||||||||||
GTCGATCATGAGTCTGCACCAGGGATCGGCGAGCGTGGAGGTGACCGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGCGTTTTTCCACAGGTGTGAGAAGTACTGAGCATTCAC > NC_002947/34862‑35153 | gTCGATCATGAGTCTGCACCAGGGATCGGCGAGCGTGGAGGTGACCGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAAt > 1:987693/1‑150 (MQ=255) tCATGAGTCTGCACCAGGGATCGGCGAGCGTGGAGGTGACCGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACg > 1:1085460/1‑150 (MQ=255) tCATGAGTCTGCACCAGGGATCGGCGAGCGTGGAGGTGACCGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACg > 1:1123770/1‑150 (MQ=255) gAGTCTGCACCAGGGATCGGCGAGCGTGGAGGTGACCGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCGATTGACGGGcc < 2:663431/150‑1 (MQ=255) gAGTCTGCACCAGGGATCGGCGAGCGTGGAGGTGACCGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCGATTGACGGGc > 1:766537/1‑149 (MQ=255) gAGTCTGCACCAGGGATCGGCGAGCGTGGAGGTGACCGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCGATTGACGGGc < 2:766537/149‑1 (MQ=255) gAGTCTGCACCAGGGATCGGCGAGCGTGGAGGTGACCGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGcc > 1:751683/1‑150 (MQ=255) cAGGGATCGGCGAGCGTGGAGGTGACCGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGc > 2:918627/1‑150 (MQ=255) aGGCATCGGCGAGCCTGCCCGCGACCGAAGAAACCTCCACCTGGTTCCACCCTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTCCACTGCGCAGACCCGATTAGGCCCGCCAACCGCCGCCCCTTTCCTTTGcc > 2:127908/1‑150 (MQ=255) aTCGGCGAGCGTGGAGGTGACCGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACAGGCCTTTTGTTTGCCTCaa < 2:657410/150‑1 (MQ=255) ggCGAGCGTGGAGGTGACCGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGc > 2:894343/1‑150 (MQ=255) tGGAGGTGACCGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGc < 1:726998/150‑1 (MQ=255) cGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGt > 2:620370/1‑138 (MQ=255) cGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGt < 1:620370/138‑1 (MQ=255) cGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACca > 2:300134/1‑150 (MQ=255) cGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCCTAAATTGCGCAGACACGATTAGGCCCGTCGATTGACGGGc > 1:894542/1‑113 (MQ=255) cGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCCTAAATTGCGCAGACACGATTAGGCCCGTCGATTGACGGGc < 2:894542/113‑1 (MQ=255) aagaaAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAg < 1:350371/143‑1 (MQ=255) aagaaAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAg > 2:350371/1‑143 (MQ=255) ttCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCAt > 2:797872/1‑150 (MQ=255) ttCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCAt > 1:168906/1‑150 (MQ=255) cACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATgg < 1:797872/150‑1 (MQ=255) ccTGGTTCCACCTTCACTTTCCGGAATCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGc < 2:265554/150‑1 (MQ=255) ccTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGa > 2:268673/1‑129 (MQ=255) ccTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGa < 1:268673/129‑1 (MQ=255) ccTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGc < 2:751683/150‑1 (MQ=255) gTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTGAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGAcc > 2:598843/1‑150 (MQ=255) aCCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGa > 2:447654/1‑150 (MQ=255) tCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGc < 1:371021/137‑1 (MQ=255) tCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGc > 2:371021/1‑137 (MQ=255) ccGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACGCGGTTAGGCCCGTCAATCGACGGGTTTCTTATTTGGCTCAAGGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACtt > 1:68775/1‑150 (MQ=255) ggAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTtaa > 1:447599/1‑150 (MQ=255) cccAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAAtc < 1:447654/150‑1 (MQ=255) cAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGAc > 1:843494/1‑105 (MQ=255) cAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGAc < 2:843494/105‑1 (MQ=255) aGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAAtcttc < 1:1033490/150‑1 (MQ=255) tgtaGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCg > 2:814964/4‑150 (MQ=255) aGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCGTCTGTTCGTCa < 2:1123770/150‑1 (MQ=255) gCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGAtt < 1:894343/150‑1 (MQ=255) aaTGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTgg > 1:1017322/1‑150 (MQ=255) aTGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATCATCTACTTTTCGTCAGGATTGGc < 2:831301/150‑1 (MQ=255) aTGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGc < 2:1017322/150‑1 (MQ=255) tGACCGCCGCTTAAATTGCGCCGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGCg < 1:1014939/150‑1 (MQ=255) tAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGCGTTTTTCCACAg < 2:168906/150‑1 (MQ=255) aaTTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGCGTTTTTCCACAGgt < 2:447599/150‑1 (MQ=255) ttGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGCGTTTTTCCACAGgtgt > 2:626592/1‑150 (MQ=255) gcgcAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACCGGGTACGTGGGCGCGCTCTGGCGTTCCCAGCTAAGTATCTTCGGTTCGTCAGGATTGGCGTTTTTCCACAGGTGTga < 1:300134/150‑1 (MQ=255) acacGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCGGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGCGTTTTTCCACAGGTGTGAGAAGTa > 1:754079/1‑150 (MQ=255) aaGGCCCGTCGATTGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGAcc < 2:723647/75‑1 (MQ=38) aaGGCCCGTCGATTGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGAcc > 1:723647/2‑76 (MQ=38) ggCCCGTCGATTGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCAc < 2:297000/84‑1 (MQ=255) ggCCCGTCGATTGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCAc > 1:297000/1‑84 (MQ=255) gCCCGTCGATTGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGCGTTTTTCCACAg > 1:344251/1‑128 (MQ=255) gCCCGTCGATTGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGCGTTTTTCCACAg < 2:344251/128‑1 (MQ=255) ccGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGCGTTTTTCca < 2:1057946/123‑1 (MQ=255) ccGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGCGTTTTTCca > 1:1057946/1‑123 (MQ=255) ccGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGCGTTTTTCCACAGGTGTGAGAAGTACTGAGCATTCAc > 1:251062/1‑150 (MQ=255) | GTCGATCATGAGTCTGCACCAGGGATCGGCGAGCGTGGAGGTGACCGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGCGTTTTTCCACAGGTGTGAGAAGTACTGAGCATTCAC > NC_002947/34862‑35153 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |