Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 399,531 A→G 8.9% V19A (GTG→GCG) ‡ PP_0332 ← acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947399,5310AG 8.9% 138.5 / 3.6 55V19A (GTG→GCG) ‡PP_0332acyltransferase
Reads supporting (aligned to +/- strand):  ref base A (22/28);  new base G (3/2);  total (25/30)
Fisher's exact test for biased strand distribution p-value = 6.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.92e-01

GGTAGGGATGCACAGGGCGAAGATGGCTTTGTCGATTTCCGACCAGGTTTCGGCCACCCACATCACCGCCCACGACGCGTAGTCGCGGCCACGGCCTGGCAACACCAGCTTGAGCAGGGCGAAGACCAGCAGCGGGCAGATCAATACCACGGTATTGAGCAGCAGCAGGGTGGTAGTGAGAATGCCGGTCAGCAGGCGACGCATAAAGAGACTCTTGTTGTGGGAGATAAACGGTTGGCAATGATAAGCAGGGCGGTGGCCTACGCCAAATGTCCAATGCCCGACGGCGGGGACAAA  >  NC_002947/399383‑399679
                                                                                                                                                    |                                                                                                                                                    
ggTAGGGATGCACAGGGCGAAGATGGCTTTGTCGATTTCCGACCAGGTTTCGGCCACCCACATCACCGCCCACGACGCGTAGTCGCGGCCACGGCCTGGCAACACCAGCTTGAGCAGGGCGAAGACCAGCAGCGGGCAGATCAATACCAc                                                                                                                                                     <  1:934722/150‑1 (MQ=255)
  tAGGGATGCACAGGGCGAAGATGGCTTTGTCGATTTCCGACCAGGTTTCGGCCACCCACATCACCGCCCACGACGCGTAGTCGCGGCCACGGCCTGGCAACACCAGCTTGAGCAGGGAGCAGCTCAGCAGCGGGCAGATCAATACCACgg                                                                                                                                                   >  1:649/1‑150 (MQ=255)
         gCACAGGGCGAAGATGGCTTTGTCGATTTCCGACCAGGTTTCGGCCACCCACATCACCGCCCACGACGCGTAGTCGCGGCCACGGCCTGGCAACACCAGCTTGAGCAGGGCGAAGACCAGCAGCGGGCAGATCAATACCACGGTATTGag                                                                                                                                            <  1:1066241/150‑1 (MQ=255)
           acaGGGCGAAGATGGCTTTGTCGATTTCCGACCAGGTTTCGGCCACCCACATCACCGCCCACGACGCGTAGTCGCGGCCACGGCCTGGCAACACCAGCTTGAGCAGGGCGAAGACCAGCAGCGGGCAGATCAATACCGTGGTATTGatct                                                                                                                                          <  1:955465/150‑4 (MQ=255)
            caGGGCGAAGATGGCTTTGTCGATTTCCGACCAGGTTTCGGCCACCCACATCACCGCCCACGACGCGTAGTCGCGGCCACGGCCTGGCAACACCAGCTTGAGCAGGGCGAAGACCAGCAGCGGGCAGATCAATACCGTGGTATTGatctg                                                                                                                                         <  2:419929/150‑5 (MQ=255)
                gCGAAGATGGCTTTGTCGATTTCCGACCAGGTTTCGGCCACCCACATCACCGCCCACGACGCGTAGTCGCGGCCACGGCCTGGCAACACCAGCTTGAGCAGGGCGAAGACCAGCAGCGGGCAGATCAATACCACGGTATTGagcagcagc                                                                                                                                     >  2:448231/1‑150 (MQ=255)
                 cGANGATGGCTTTGTCGATTTCCGACCAGGTTTCGGCCACCCACATCACCGCCCACGACGCGTAGTCGCGGCCACGGCCTGGCAACACCAGCTTGAGCAGGGCGAAGACCAGCAGCGGGCAGATCAATACCACGGTATTGAgcagcagca                                                                                                                                    >  2:693861/1‑150 (MQ=255)
                  gAAGATGGCTTTGTCGATTTCCGACCAGGTTTCGGCCACCCACATCACCGCCCACGACGCGTAGTCGCGGCCACGGCCTGGCAACACCAGCTTGAGCAGGGCGAAGACCAGCAGCGGGCAGATCAATACCACGGTATTGagcagca                                                                                                                                       <  1:153856/146‑1 (MQ=255)
                  gAAGATGGCTTTGTCGATTTCCGACCAGGTTTCGGCCACCCACATCACCGCCCACGACGCGTAGTCGCGGCCACGGCCTGGCAACACCAGCTTGAGCAGGGCGAAGACCAGCAGCGGGCAGATCAATACCACGGTATTGagcagca                                                                                                                                       >  2:153856/1‑146 (MQ=255)
                   aaGATGGCTTTGTCGATTTCCGACCAGGTTTCGGCCACCCACATCACCGCCCACGACGCGTAGTCGCGGCCACGGCCTGGCAACACCAGCTTGAGCAGGGCGAAGACCAGCAGCGGGCAGATCAATACCACGGTATTGAGCAGCAGCAgg                                                                                                                                  <  2:837645/150‑1 (MQ=255)
                      aTGGCTTTGTCGATTTCCGACCAGGTTTCGGCCACCCACATCACCGCCCACGACGCGTAGTCGCGGCCACGGCCTGGCAACACCAGCTTGAGCAGGGCGAAGACCAGCAGCGGGCAGATCAATACCACGGTCTTGCGCAGCGGCACggtt                                                                                                                               <  2:103321/150‑2 (MQ=255)
                      aTGGCTTTGTCGATTTCCGACCAGGTTTCGGCCACCCACATCACCGCCCACGACGCGTAGTCGCGGCCACGGCCTGGCAACACCAGCTTGAGCAGGGCGAAGACCAGCAGCGGGCAGATCAATACCACGGTATTGAGCAGCAGCAGggtg                                                                                                                               <  1:693861/150‑1 (MQ=255)
                      aTGGCTTTGTCGATTTCCGACCAGGTTTCGGCCACCCACATCACCGCCCACGACGCGTAGTCGCGGCCACGGCCTGGCAACACCAGCTTGAGCAGGGCGAAGACCAGCAGCGGGCAGATCAATACCACGGTATTGAGCAGCAGCAGggtg                                                                                                                               <  1:794821/150‑1 (MQ=255)
                       tGGCTTTGTCGATTTCCGACCAGGTTTCGGCCACCCACATCACCGCCCACGACGCGTAGTCGCGGCCACGGCCTGGCAACACCAGCTTGAGCAGGGCGAAGACCAGCAGCGGGCAGATCAATACCACGGTATTGAGCAGCAGCAGggtgg                                                                                                                              <  1:859156/150‑1 (MQ=255)
                         gCTTTGTCGATTTCCGACCAGGTTTCGGCCTCCCACATCACCGCCCACGACGCGTAGTCGCGGCCACGGCCTGGCAACACCAGCTTGAGCAGGGGGAAGACCAGCAGCGGGCAGATCAATACCACGGTATTGAGCAGCAGCAGGGTGGTa                                                                                                                            <  2:596865/150‑1 (MQ=255)
                               tCGATTTCCGACCAGGTTTCGGCCACCCACATCACCGCCCACGACGCGTAGTCGCGGCCACGGCCTGGCAACACCAGCTTGAGCAGGGCGAAGACCAGCAGCGGGCAGATCAATACCACGGTATTGAGCAGCAGCAGGGTGGTAGTgaga                                                                                                                      >  2:816522/1‑150 (MQ=255)
                                 gATTTCCGACCAGGTTTCGGCCACCCACATCACCGCCCACGACGCGTAGTCGCGGCCACGGCCTGGCAACACCAGCTTGAGCAGGGCGAAGACCAGCAGCGGGCAGATCAATACCACGGTATTGAGCAGCAGCAGggt                                                                                                                                >  1:449379/1‑138 (MQ=255)
                                 gATTTCCGACCAGGTTTCGGCCACCCACATCACCGCCCACGACGCGTAGTCGCGGCCACGGCCTGGCAACACCAGCTTGAGCAGGGCGAAGACCAGCAGCGGGCAGATCAATACCACGGTATTGAGCAGCAGCAGggt                                                                                                                                <  2:449379/138‑1 (MQ=255)
                                    ttCCGACCAGGTTTCGGCCACCCACATCACCGCCCACGACGCGTAGTCGCGGCCACGGCCTGGCAACACCAGCTTGAGCAGGGCGAAGACCAGCAGCGGGCAGATCAATATCGCGGTATTGAGCAGCAGCAGGGTGGTAGTGAGAATGcc                                                                                                                 >  1:57565/1‑150 (MQ=255)
                                    ttCCGACCAGGTTTCGGCCACCCACATCACCGCCCACGACGCGTAGTCGCGGCCACGGCCTGGCAACACCAGCTTGAGCAGGGCGAAGACCAGCAGCGGGCAGATCAATACCACGGTATTGAGCAGCAGCAGGGTGGTAGTGAGAATGcc                                                                                                                 <  2:863909/150‑1 (MQ=255)
                                                    gCCACCCACATCACCGCCCACGACGCGTAGTCGCGGCCACGGCCTGGCAACACCAGCTTGAGCAGGGCGAAGACCAGCAGCGGGCAGATCAATACCACGGTATTGAGCAGCAGCAGGGTGGTAGTGAGAATGCCGGTCAGCAGGCGACGc                                                                                                 >  1:651585/1‑150 (MQ=255)
                                                       acccacATCACCGCCCACGACGCGTAGTCGCGGCCACGGCCTGGCAACACCAGCTTGAGCAGGGCGAAGACCAGCAGCGGGCAGATCAATACCACGGTATTGAGCAGCAGCAGGGTGGTAGTGAGAATGCCGGTCAGCAGGCGACGCATa                                                                                              >  1:743134/1‑150 (MQ=255)
                                                         ccacATCACCGCCCACGACGCGTAGTCGCGGCCACGGCCTGGCAACACCAGCTTGAGCAGGGCGAAGACCAGCAGCGGGCAGATCAATACGAGGAGAGTGAGCAGCAGCAGGGTGGTAGTGAGAATGCCGGTCAGCAGGCGACGCATaaa                                                                                            >  1:11595/1‑150 (MQ=255)
                                                         ccacATCACCGCCCACGACGCGTAGTCGCGGCCACGGCCTGGCAACACCAGCTTGAGCAGGGCGAAGACCAGCAGCGGGCAGATCAATACCACGGTATTGAGCAGCAGCAGGGTGGTAGTGAGAATGCCGGTCAGCAGGCGACGCATaaa                                                                                            >  2:520449/1‑150 (MQ=255)
                                                          cacaTCACCTCCCACGACGCGTAGTCGCGGCCACGGCCTGGCAACACCAGCTTGAGCAGGGCGAAGACCAGCAGCGGGAAGTTCAATACCACGGTATTGAGCAGCAGCAGGGTGGTAGTGAGAATGCCGGTCAGCAGGCGACGCATAAag                                                                                           <  1:629630/150‑1 (MQ=255)
                                                           acaTCACCGCCCACGACGCGTAGTCGCGGCCACGGCCTGGCAACACCAGCTTGAGCAGGGCGAAGACCAGCAGCGGGCAGATCAATACCACGGTATTGAGCAGCAGCAGGGTGGTAGTGAGAATGCGGGTCAGCAGGCGACGCATAAaga                                                                                          >  1:298381/1‑150 (MQ=255)
                                                           acaTCACCGCCCACGACGCGTAGTCGCGGCCACGGCCTGGCAACACCAGCTTGAGCAGGGCGAAGACCAGCAGCGGGCAGATCAATACCACGGTATTGAGCAGCAGCAGGGTGGTAGTGAGAATGCCGGTCAGCAGGCGACGCATAAaga                                                                                          >  2:363158/1‑150 (MQ=255)
                                                           acaTCACCGCCCACGACGCGTAGTCGCGGCCACGGCCTGGCAACACCAGCTTGAGCAGGGCGAAGACCAGCAGCGGGCAGATCAATACCACGGTATTGAGCAGCAGCAGGGTGGTAGTGAGAATGCAGGTCAGCAGGCGACGCATAAaga                                                                                          >  1:298208/1‑150 (MQ=255)
                                                           acaTCACCGCCCACGACGCGTAGTCGCGGCCACGGCCTGGCAACACCAGCTTGAGCAGGGCGAAGACCAGCAGCGCGCACTCCAATACCACGGTATTGAGCAGCAGCAGGGTGGTAGTGAGAATGCCGGTCAGCAGGCGACGCATAAaga                                                                                          >  1:1040363/1‑150 (MQ=255)
                                                             aTCACCGCCCACGACGCGTAGTCGCGGCCACGGCCTGGCAACACCAGCTTGAGCAGGGCGAAGACCAGCAGCGGGCAGATCAATACCACGGTATTGAGCAGCAGCAGGGTGGTAGTGAGAATGCCGGTCAGCAGGCGACGCATAAAgaga                                                                                        <  1:363158/150‑1 (MQ=255)
                                                             aTCACCGCCCACGACGCGTAGTCGCGGCCACGGCCTGGCAACACCAGCTTGAGCAGGGCGAAGACCAGCAGCGGGCAGATCAATACCACGGTATTGAGCAGCAGCAGGGTGGTAGTGAGAATGCCGGTCAGCAGGCGACGCATAAAgaga                                                                                        <  1:520449/150‑1 (MQ=255)
                                                                 ccGCCCCCGACGCGTAGTCGCGGCCACGGCCAGGCAACACCGTCGTGAGCAGGGCGAAGACCAGCAGCGGGCAGATCAATACCACGGTATTGAGCAGCAGCAGGGTGGTAGTGAGAATGCCGGTCAGCAGGCGACGCATAAAGAGActct                                                                                    >  2:739576/1‑150 (MQ=255)
                                                                      cACGACGCGTAGTCGCGGCCACGGCCTGGCAACACCAGCTTGAGCAGGGCGAAGACCAGCAGCGGGCAGATCAATACCACGGTATTGAGCAGCAGCAGGGTGGTAGTGAGAATGCCGGTCAGCAGGCGACGCATAAAGAGACTCttgttg                                                                               <  2:57565/150‑1 (MQ=255)
                                                                                 gTCGCGGCCACGGCCTGGCAACACCAGCTTGAGCAGGGCGAAGACCAGCAGCGGGCAGATCAATACCACGGTATTGAGCAGCAGCAGGGTGGTAGTGAGAATGCCGGTCAGCAGGCGACGCATAAAGAGACTCTTGTTGTGGGAGATaaa                                                                    <  2:952204/150‑1 (MQ=255)
                                                                                      ggCCACGGCCTGGCAACACCAGCTTGAGCAGGGCGAAGACCAGCAGCGGGCAGATCAATACCACGGTATTGAGCAGCAGCAGGGTGGTAGTGAGAATGCCGGTCAGCAGGCGACGCATAAAGAGATTCTTGTTGTGGGAGATAAACGGtt                                                               <  1:705481/150‑1 (MQ=255)
                                                                                                    aaCACCAGCTTGAGCAGGGCGAAGACCAGCAGCGGGCAGATCAATACCACGGTATTGAGCAGCAGCAGGGTGGTAGTGAGAATGCCGGTCAGCAGGCGACGCATAAAGAGACTCTTGTTGTGGGAGATAAACGGTTGGCAATGATAAGCa                                                 >  2:140930/1‑150 (MQ=255)
                                                                                                     acacCAGCTTGAGCAGGGCGAAGACCAGCAGCGGGCAGATCAATACCACGGTATTGAGCAGCAGCAGGGTGGTAGTGAGAATGCCGGTCAGCAGGCGACGCATAAAGAGACTCTTGTTGTGGGAGATAAACGGTTGGCAATGATAAGCAg                                                >  2:92585/1‑150 (MQ=255)
                                                                                                     acacCAGCTTGAGCAGGGCGAAGACCAGCAGCGGGCAGATCAATACCACGGTATTGAGCAGCAGCAGGGTGGTAGTGAGAATGCCGGTCAGCAGGCGACGCATAAAGAGACTCTTGTTGTGGGAGATAAACGGTTGGCAATGATAAGCAg                                                <  1:92585/150‑1 (MQ=255)
                                                                                                     acacCAGCTTGAGCAGGGCGAAGACCAGCAGCGGGCAGATCAATACCACGGTATTGAGCAGCAGCAGGGTGGTAGTGAGAATGCCGGTCAGCAGGCGACGCATAAAGAGACTCTTGTTGTGGGAGATAAACGGTTGGCAATGATAAGCAg                                                <  1:610863/150‑1 (MQ=255)
                                                                                                        ccAGCTTGAGCAGGGCGAAGACCAGCAGCGGGCAGATCAATACCACGGTATTGAGCAGCAGCAGGGTGGTAGTGAGAATGCCGGTCAGCAGGCGACGCATAAAGAGACTCTTGTTGTGGGAGATAAACGGTTGGCAATGATAAGCAGGGc                                             >  1:605466/1‑150 (MQ=255)
                                                                                                         cAGCTTGAGCAGGGCGAAGACCAGCAGCGGGCAGATCAATACCACGGTATTGagca                                                                                                                                          >  2:197343/1‑56 (MQ=255)
                                                                                                         cAGCTTGAGCAGGGCGAAGACCAGCAGCGGGCAGATCAATACCACGGTATTGagca                                                                                                                                          <  1:197343/56‑1 (MQ=255)
                                                                                                         cAGCTTGAGCAGGGCGAAGACCAGCAGCGGGCAGATCAATACCACGGTATTGAGCAGCAGCAGGGTGGTAGTGAGAATGCCGGTCAGCAGGCGACGCATAAAGAGACTCTTGTTGTGGGAGATAAACGGTTGGCACTGATAAGAAGGGCg                                            <  2:743134/150‑1 (MQ=255)
                                                                                                         cAGCTTGAGCAGGGCGAAGACCAGCAGCGGGCAGATCAATACCACGGTATTGAGCAGCAGCAGGGTGGTAGTGAGAATGCCGGTCAGCAGGCGACGCATAAAGAGACTCTTGTTGTGGGAGATAAACGGTTGGCAATGATAAGCAGGGCg                                            >  1:1088057/1‑150 (MQ=255)
                                                                                                            cTTGAGCAGGGCGAAGACCAGCAGCGGGCAGATCAATACCACGGTATTGAGCAGCAGCAGGGTGGTAGTGAGAATGCCGGTCAGCAGGCGACGCATAAAGAGACTCTTGTTGTGGGAGATAAACGGTTGGCAATGATAAGCAGGGCGGTg                                         <  2:914489/150‑1 (MQ=255)
                                                                                                                  cAGGGCGAAGACCAGCAGCGGGCAGATCAATACCACGGTATTGAGCAGCAGCAGGGTGGTAGTGAGAATGCCGGTCAGCAGGCGACGCATAAAGAGACTCTTGTTGTGGGAGATAAACGGTTGGCAATGATAAGCAGGGCGGTGGCCTAc                                   <  2:1040363/150‑1 (MQ=255)
                                                                                                                        gAAGACCAGCAGCGGGCAGATCAATACCACGGTATTGAGCAGCAGCAGGGTGGTAGTga                                                                                                                        >  1:322553/1‑59 (MQ=255)
                                                                                                                        gAAGACCAGCAGCGGGCAGATCAATACCACGGTATTGAGCAGCAGCAGGGTGGTAGTga                                                                                                                        <  2:322553/59‑1 (MQ=255)
                                                                                                                        gAAGACCAGCAGCGGGCAGATCAATACCACGGTATTGAGCAGCAGCAGGGTGGTAGTGAGAATGCCGGTCAGCAGGCGACGCATAAAGAGACTCTTGTTGTGGGAGATAAACGGTTGGCAATGATAAGCAGGGCGGTGGCCTACGCCaaa                             <  1:448231/150‑1 (MQ=255)
                                                                                                                               agcagcGGGCAGATCAATACCACGGTATTGAGCAGCAGCAGGGTGGTAGTGAGAATGCCGGTCAGCAGGCGACGCATAAAGAGACTCTTGTTGTGGGAGATAAACGGTTGGCAATGATAAGCAGGGCGGTGGCCTACGCCAAATGTCCaa                      <  2:27447/150‑1 (MQ=255)
                                                                                                                                           ctCAATACCGTGGTATTGAGCAGCAGCAGGGTGGTAGTGAGAATGCCGGTCAGCAGGCGACGCATAAAGAGACTCTTGTTGTGGGAGATAAACGGTTGGCAATGATAAGCAGGGCGGTGGCCTACGCCAAATGTCCAATGCCCGAcggcg          >  1:835453/2‑150 (MQ=255)
                                                                                                                                           ctCAATACCGTGGTATTGAGCAGCAGCAGGGTGGTAGTGAGAATGCCGGTCAGCAGGCGACGCATAAAGAGACTCTTGTTGTGGGAGATAAACGGTTGGCAATGATAAGCAGGGCGGTGGCCTACGCCAAATGTCCAATGCCCGAcggcg          >  1:904550/2‑150 (MQ=255)
                                                                                                                                            tCAATACCACGGTATTGAGCAGCAGCAGGGTGGTAGTGAGAATGCCGGTCAGCAGGCGACGCATAAAGAGACTCTTGTTGTGGGAGATAAACGGTTGGCAATGATAAGCAGGGCGGTGGCCTACGCCAAATGTCCAATGCCCGAcggcg          <  1:752905/149‑1 (MQ=255)
                                                                                                                                            tCAATACCACGGTATTGAGCAGCACCAGGGTGGTAGTGAGAATGCCGGTCAGCAGGCGACGCATAAAGAGACTCTTGTTGTGGGAGATAAACGGTTGGCAATGATAAGCAGGGCGGTGGCCTACGCCAAATGTCCAATGCCCGAcggcg          >  2:752905/1‑149 (MQ=255)
                                                                                                                                                   cACGGTATTGAGCAGCAGCAGGGTGGTAGTGAGAATGCCGGTCAGCAGGCGACGCATAAAGAGACTCTTGTTGTGGGAGATAAACGGTTGGCAATGATAAGCAGGGCGGTGGCCTACGCCAAATGTCCAATGCCCGACGGCGGGGACaaa  <  1:816522/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GGTAGGGATGCACAGGGCGAAGATGGCTTTGTCGATTTCCGACCAGGTTTCGGCCACCCACATCACCGCCCACGACGCGTAGTCGCGGCCACGGCCTGGCAACACCAGCTTGAGCAGGGCGAAGACCAGCAGCGGGCAGATCAATACCACGGTATTGAGCAGCAGCAGGGTGGTAGTGAGAATGCCGGTCAGCAGGCGACGCATAAAGAGACTCTTGTTGTGGGAGATAAACGGTTGGCAATGATAAGCAGGGCGGTGGCCTACGCCAAATGTCCAATGCCCGACGGCGGGGACAAA  >  NC_002947/399383‑399679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑