Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,811,491 C→T 8.0% Q150* (CAG→TAG)  dsbG → protein disulfide isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,811,4910CT 8.0% 132.9 / 2.9 50Q150* (CAG→TAG) dsbGprotein disulfide isomerase
Reads supporting (aligned to +/- strand):  ref base C (21/25);  new base T (2/2);  total (23/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.00e-01

ATCTGGGCGAAAATGGAAAAAACCGCGTGGATCGCCGACGGCAAGGCCGATGCGCCGCGCAAGGTGTACCTGTTCAGCGACCCGAACTGCCCGTACTGCAACATGTTCTGGGAACAGGCGCGGCCCTGGGTGGAGTCGGGCAAGGTGCAGTTGCGCCATATCATGGTCGGCATCATCCGCGAGGACAGCCCGGGCAAGTCGGCGGCGCTGCTGGCGGCGAAAGACCCGGTCAAGGCCCTGCAGCAGCATGAAAAGGCCGGCAAGGCCAGCACGCTGAAGGCACTCGAGCAGGTGCC  >  NC_002947/4811344‑4811639
                                                                                                                                                   |                                                                                                                                                    
aTCTGGGCGAAGATGGAAAAAACCGCGTGGATCGCCGACGGCAAGGCCGATGCGCCGCGCAAGGTGTACCTGTTCAGCGACCCGAACTGCCCGTACTGCAACATGTTCTGGGAACAGGCGCGGCCCTGGGTGGAGTCGGGCAAGGTGCAg                                                                                                                                                    <  2:26137/150‑1 (MQ=255)
aTCTGGGCGAAAATGGAAAAAACCGCGTGGATCGCCGACGGCAAGGCCGATGCGCCGCGCAAGGTGTACCTGTTCAGCGACCCGAACTGCCCGTACTGCAACATGTTCTGGGAACAGGCGCGGCCCTGGGTGGAGTCGGGCAAGGTGCAg                                                                                                                                                    <  1:194293/150‑1 (MQ=255)
 tCTGGGCGAAAATGGAAAAAACCGCGTGGATCGCCGACGGCAAGGCCGATGCGCCGCGCAAGGTGTACCTGTTCAGCGACCCGAACTGCCCGTACTGCAACATGTTCTGGGAACAGGCGCGGCCCTGGGTGGAGTCGGGCAAGGTGTAGt                                                                                                                                                   >  1:1023169/1‑150 (MQ=255)
 tCTGGGCGAAAATGGAAAAAACCGCGTGGATCGCCGACGGCAAGGCCGATGCGCCGCGCAAGGTGTACCTGTTCAGCGACCCGAACTGCCCGTACTGCAACATGTTCTGGGAACAGGCGCGGCCCTGGGTGGAGTCGGGCAAGGTGTAGt                                                                                                                                                   >  1:1023101/1‑150 (MQ=255)
    gggCGAAAATGGAAAAAACCGCGTGGATCGCCGACGGCAAGGCCGATGCGCCGCGCAAGGTGTACCTGTTCAGCGACCCGAACTGCCCGTACTGCAACATGTTCTGGGAACAGGCGCGGCCCTGGGTGGAGACGGGCAAGGTGCGGtagg                                                                                                                                                >  2:727146/1‑147 (MQ=255)
     ggCGAAAATGGAAAAAACCGCGTGGATCGCCGACGGCAAGGCCGATGCGCCGCGCAAGGTGTACCTGTTCAGCGACCGGAACTGCCCGTACTGCAACATGTTCTGGGAACAGGCGCGGCCCTGGGTGGAGTCGGGCAAGGTGCAGTTgcg                                                                                                                                               >  2:45730/1‑150 (MQ=255)
     ggCGAAAATGGAAAAAACCGCGTGGATCGCCGACGACAAGGCCGATGCGCCGCGCAAGGTGTACCTGTTCAGCGACCCGAACTGCCCGTACTGCAACATGTTCTGGGAACAGGCGCGGCACTGGGTGGAGTCGGGCAAGGTGCAGTTgcg                                                                                                                                               >  2:595719/1‑150 (MQ=255)
                       cgcgTGGATCGCCGACGGCAAGGCCGATGCGCCGCGCAAGGTGTACCTGTACAGCGACACGAACTGCCCATAATGAAAAATGTTGTGGGAACAGGTGCTGCCCTTGGTGTTGTCGGGCAAGGTGCAGTTGCGCCATATCATGGTCGGcat                                                                                                                             >  2:124707/1‑150 (MQ=255)
                               tCGCCGACGGCAAGGCCGATGCAGTGTGCAAGGTGTACCTGTTCAGCGACCCGAACTGCCCGTACTGCAACATGTTCTGGGAACAGGCGCGGCCCTGGGTGGAGTCGGGCAAGGTGCAGTTGCGCCATATCATGGTCGGCATCATCcgcg                                                                                                                     >  1:996980/1‑150 (MQ=255)
                                cGCCGACGGCAAGGCCGATGCGCCGCGCAAGGTGTACCTGTTCAGCGACCCGAACTGCCCGTACTGCAACATGTTCTGGGAACAGGCGCGGCCCTGGGTGGAGTCGGGCAAGGTGCAGTTGCGCCATATCATGGTCGGCATCATCCGCGa                                                                                                                    <  1:164068/150‑1 (MQ=255)
                                   cGACGGCAAGGCCGATGCGCCGCGCAAGGTGTACCTGTTCAGCGACCCGAACTGCCCGTACTGCAACATGTTCTGGGAACAGGCGCGGCCCTGGGTGGAGTCGGGCAAGGTGCAGTTGCGCCATATCATGGTCg                                                                                                                                 >  1:1099883/1‑134 (MQ=255)
                                   cGACGGCAAGGCCGATGCGCCGCGCAAGGTGTACCTGTTCAGCGACCCGAACTGCCCGTACTGCAACATGTTCTGGGAACAGGCGCGGCCCTGGGTGGAGTCGGGCAAGGTGCAGTTGCGCCATATCATGGTCg                                                                                                                                 <  2:1099883/134‑1 (MQ=255)
                                    gACGGCAAGGCCGATGCGCCGCGCAAGGTGTACCTGTTCAGCGACCCGAACTGCCCGTACTGCAACATGTTCTGGGAACAGGCGCGGCCCTGGGTGGAGTCGGGCAAGGTGCAGTTGCACCATATCATGGTCGGCATCATCCGCGAGGAc                                                                                                                >  2:139437/1‑150 (MQ=255)
                                                           cAAGGTGTACCTGTTCAGCGACCCGAACTGCCCGTACTGCAACATGTTCTGGGAACAGGCGCGGCCCTGGGTGGAGTCGGGCAAGGTGCAGTTGCGCCATATCATGGTCGGCATCATCCGCGAGGACAGCCCGGGCAAGTCGGCGGCgct                                                                                         >  1:655094/1‑150 (MQ=255)
                                                               gtgtACCTGTTCAGCGACCCGAACTGCCCGTACTGCAACATGTTCTGGGAACAGGCGCGGCCCTGGGTGGAGTCGGGCAAGGTGCAGTTGCGCCATATCATGGTCGGCATCATCCGCGAGGACAGCCCGGGCAAGTCGGCGGCGctgctg                                                                                     >  1:863934/1‑150 (MQ=255)
                                                                  tACCTGTTCAGCGACCCGAACTGCCCGTACTGCAACATGTTCTGGGAACAGGCGCGGCCCTGGGTGGAGTCGGGCAAGGTGCAGTTGCGCCATATCATGGTCGGCATCATCCGCGAGGACAGCCCGGGCAAGTCGGCGGCGCTGCTggcg                                                                                  >  2:623211/1‑150 (MQ=255)
                                                                    ccTGTTCAGCGACCCGAACTGCCCGTACTGCAACATGTTCTGGGAACAGGCGCGGCCCTGGGTGGAGTCGGGCAAGGTGCAGTTGCGCCATATCATGGTCGGCATCATCCGCGAGGACAGCCCGGGCAAGTCGGCGGCGCTGCTggcggc                                                                                <  1:682873/150‑1 (MQ=255)
                                                                     cTGTTCAGCGACCCGAACTGCCCGTACTGCAACATGTTCTGGGAACAGGCGCGGCCCTGGGTGGAGTCGGGCAAGGTGCAGTTGCGCCATATCATGGTCGGCATCATCCGCGAGGACAGCCCGGGCAAGTCGGCGGCGCTGCTGgcggcg                                                                               >  2:320397/1‑150 (MQ=255)
                                                                      tGTTCAGCGACCCGAACTGCCCGTACTGCAACATGTTCTGGGAACAGGCGCGGCCCTGGGTGGAGTCGGGCAAGGTGCAGTTGCGCCATATCATGGTCGGCATCATCCGCGAGGACAGCCCGGGCAAGTCGGCGGCGCTGCTGGCGGCGa                                                                              >  1:1089752/1‑150 (MQ=255)
                                                                          cAGCGACCCGAACTGCCCGTACTGCAACATGTCCTGGGAACAGGCGCGGCCCTGGGTGGAGTCGGGCAAGGTGCAGTTGCGCCATATCATGGTCGGCATCATCCGCGAGGACAGCCCGGGCAAGTCGGCGGCGCTGCTGGCGGCGAAAGa                                                                          <  1:961283/150‑1 (MQ=255)
                                                                              ggcccGAACTGCCCGTACTGCAACATGTTCTGGGAACAGGCGCGGCCCTGGGTGGAGTCGGGCAAGGTGCAGTTGCGCCATATCATGGTCGGCATCATCCGCGAGGACAGCCCGGGCAAGTCGGCGGCGCTGCTGGCGGCGAAAGACCCg                                                                      <  1:210243/148‑1 (MQ=255)
                                                                               aCCCGAACTGCCCGTACTGCAACATGTTCTGGGAACAGGCGCGGCCCTGGGTGGAGTCGGGCAAGGTGCAGTTGCGCCATATCATGGTCGGCATCATCCGCGAGGACAGCCCGGGCAAGTCGGCGGCGCTGCTGGCGGCGAAAGACCCgg                                                                     >  2:830495/1‑150 (MQ=255)
                                                                                  cGAACTGCCCGTACTGCAACATGTTCTGGGAACAGGCGCGGCCCTGGGTGGAGTCGGGCAAGGTGCAGTCGCGCCATATCATGGTCGGCATCATCCGCGAGGACAGCCCGGGCAAGTCGGCGGCGCTGCTGGCGGCGAAAGACCCGGTCa                                                                  >  2:572615/1‑150 (MQ=255)
                                                                                    aaCTGCCCGTACTGCAACATGTTCTGGGAACAGGCGCGGCCCTGGGTGGAGTCGGGCAAGGTGCAGTTGCGCCATATCATGGTCGGCATCATCCGCGAGGACAGCCCGGGCAAGTCGGCGGCGCTGCTGGCGGCGAAAGACCCGGTCAAg                                                                <  1:830495/150‑1 (MQ=255)
                                                                                      cTGCCCGTACTGCAACATGTTCTGGGAACAGGCGCGGCCCTGGGTGGAGTCGGGCAAGGTGCAGTTGCGCCATATCATGGTCGGCATCATCCGCGAGGACAGCCCGGGCAAGTCGGCGGCGCTGCTGGCGGCGAAAGACCCGGTCAAGGc                                                              >  2:1058671/1‑150 (MQ=255)
                                                                                              aCTGCAACATGTTCTGGGAACAGGCGCGGCCCTGGGTGGAGTCGGGCAAGGTGCAGTTGCGCCATATCATGGTCGGCATCATCCGCGAGGACAGCCCGGGCAAGTCGGCGGCGCTGCTGGCGGCGAAAGACCCGGTCAAGGCCCTgcagc                                                      <  1:623211/150‑1 (MQ=255)
                                                                                                tGCAACATGTTCTGGGAACAGGCGCGGCCCTGGGTGGAGTCGGGCAAGGTGCAGTTGCGCCATATCATGGTCGGCATCATCCGCGAGGACAGCCCGGGCAAGTCGGCGGCGCTGCTGGCGGCGAAAGACCCGGTCAAGGCCCTgcagcag                                                    <  2:1089752/150‑1 (MQ=255)
                                                                                                 gCAACTTGTTCTGGGAACAGGCGCGGCCCTGGGTGGAGTCGGGCAAGGTGCAGTTGCGCCATATCATGGTCGGCATCATCCGCGAGGACAGCCCGGGCAAGTCGGCGGCGCTGCTGGCGGCGAAAGACCCGGTCAAGGCCCTgcagcagc                                                   <  2:1041375/150‑1 (MQ=255)
                                                                                                 gCAACATGTTCTGGGAACAGGCGCGGCCCTGGGTGGAGTCGGGCAAGGTGCAGTTGCGCCATATCATGGTCGGCATCATCCGCGAGGACAGCCCGGGCAAGTCGGCGGCGCTGCTGGCGGCGAAAGACCCGGTCAAGGCCCTgcagcagc                                                   <  2:777284/150‑1 (MQ=255)
                                                                                                  cAACATGTTCTGGGAACAGGCGCGGCCCTGGGTGGAGTCGGGCAAGGTGCAGTTGCGCCATATCATGGTCGGCATCATCCGCGAGGACAGCCCGGGCAAGTCGGCGGCgctgct                                                                                      >  2:81967/1‑114 (MQ=255)
                                                                                                  cAACATGTTCTGGGAACAGGCGCGGCCCTGGGTGGAGTCGGGCAAGGTGCAGTTGCGCCATATCATGGTCGGCATCATCCGCGAGGACAGCCCGGGCAAGTCGGCGGCgctgct                                                                                      <  1:81967/114‑1 (MQ=255)
                                                                                                   aaCATGTTCTGGGAACAGGCGCGGCCCTGGGTGGAGTCGGGCAAGGTGCAGTTGCGCCATATCATGGTCGGCATCATCCGCGAGGACAGCCCGGGCAAGTCGGCGGCGCTGCTGGCGGCGAAAGACCCGGTCAAGGCCCTGCAGCAGCAt                                                 >  1:530919/1‑150 (MQ=255)
                                                                                                            tGGGAACAGGCGCGGCCCTGGGTGGAGTCGGGCAAGGTGCAGTTGCGCCATATCATGGTCGGCATCATCCGCGAGGACAGCCCGGGCAAGTCGGCGGCGCTGCTGGCGGCGAAAGACCCGGTCAAGGCCCTGCAGCAGCATGAAAAGGcc                                        <  1:572615/150‑1 (MQ=255)
                                                                                                               gAACAGGCGCGGCCCTGGGTGGAGTCGGGCAAGGTGCAGTTGCGCCATATCATGGTCGGCATCATCCGCGAGGACAGCCCGGGCAAGTCGGCGGCGCTGCTGGCGGCGAAAGACCCGGTCAAGGCCCTGCAGCAGCATGAAAAGGCCGGc                                     <  2:655094/150‑1 (MQ=255)
                                                                                                                aaCAGGCGCGGCCCTGGGTGGAGTCGGGCAAGGTGCAGTTGCGCCATATCATGGTCGGCATCATCCGCGAGGACAGCCCGGGCAAGTCGGCGGCGCTGCTGGCGGCGAAAGACCCGGTCAAGGCCCTGCAGCAGCATGAAAAGGCCGGCa                                    >  2:668832/1‑150 (MQ=255)
                                                                                                                    ggCGCGGCCCTGGGTGGAGTCGGGCAAGGTGCAGTTGCGCCATATCATGGTCGGCATCATCCGCGAGGACAGCCCGGGCAAGTCGGCGGCGCTGCTGGCGGCGAAAGACCCGGTCAAGGCCCTGCAGCAGCATGAAAAGGCCGGCAAGGc                                <  2:660212/150‑1 (MQ=255)
                                                                                                                       gcgGCCCTGGGGGGAGTCGGGCAAGGTGCAGTTGCGCCATATCATGGTCGGCATCATCCGCGAGGACAGCCCGGGCAAGTCGGCGGCGCTGCTGGCGGCGAAAGACCCGGTCAAGGCCCTGCAGCAGCATGAAAAGGCCGGCAAGGCCAg                             <  2:410842/150‑1 (MQ=255)
                                                                                                                            ccTGGGTGGAGTCGGGCAAGGTGCAGTTGCGCAATATCATGGTCGGCATCATCCGAGAGGACAGCCCGGGCAAGTCGGCGGCGCTGCTGGCGGCGAAAGACCCGGTCAAGGCCCTGCAGCAGCATGAAAAGGCCGGCAAGGCCAGCACGc                        <  2:530919/150‑1 (MQ=255)
                                                                                                                             cTGGGTGGAGTCGGGCAAGGTGCAGTTGCGCCATATCATGGTCGGCATCATCCGCGAGGACAGCCCGGGCAAGTCGGCGGCGCTGCTGGCGGCGTATAACCCGGTCAAGGCCCTGCAGTAGCATGAAAAGGCCGGCAAGGCCAGCACGCt                       <  1:139437/150‑1 (MQ=255)
                                                                                                                                ggTGGAGTCGGGCAAGGTGCAGTTGCGCCATATCATGGTCGGCATCATCCGCGAGGACAGCCCGGGCAAGTCGGCGGCGCTGCTGGCGGCGAAAGACCCGGTCAAGGCCCTgc                                                         <  1:781581/113‑1 (MQ=255)
                                                                                                                                ggTGGAGTCGGGCAAGGTGCAGTTGCGCCATATCATGGTCGGCATCATCCGCGAGGACAGCCCGGGCAAGTCGGCGGCGCTGCTGGCGGCGAAAGACCCGGTCAAGGCCCTgc                                                         >  2:781581/1‑113 (MQ=255)
                                                                                                                                ggTGGAGTCGGGCAAGGTGCAGTTGCGCCATATCATGGTCGGCATCATCCGCGAGGACAGCCCGGGCAAGTCGGCGGCGCTGCTGGCGGCGAAAGACCCGGTCAAGGCCCTGCAGCAGCATGAAAAGGCCGGCAAGGCCAGCACGCTGaa                    <  1:320397/150‑1 (MQ=255)
                                                                                                                                ggTGGAGTCGGGCAAGGTGCAGTTGCGCCATATCATGGTCGGCATCATCCGCGAGGACAGCCCGGGCAAGTCGGCGGCGCTGCTGGCGGCGAAAGACCCGGTCAAGGCCCTGCAGCAGCATGAAAAGGCCGGCAAGGCCAGCACGCTGaa                    <  1:45730/150‑1 (MQ=255)
                                                                                                                                ggTGGAGTCGGGCAAGGTGCAGTTGCGCCATATCATGGTCGGCATCATCCGCGAGGACAGCCCGGGCAAGTCGGCGGCGCTGCTGGCGGCGAAAGACCCGGTCAAGGCCCTGCAGCAGCATGAAAAGGCCGGCAAGGCCAGCACGCTGaa                    <  1:595719/150‑1 (MQ=255)
                                                                                                                                 gTGGAGTCGGGCAAGGTGTAGTTGCGCCATATCATGGTCGGCATCATCCGCGAGGACAGCCCGGGCAAGTCGGCGGCGCTGCTGGCGGCGAAAGACCCGGTCAAGGCCCTGCAGCAGCATGAAAAGGCCGGCAAGGCCAGCACGCTGAAg                   <  2:1023101/150‑1 (MQ=255)
                                                                                                                                 gTGGAGTCGGGCAAGGTGTAGTTGCGCCATATCATGGTCGGCATCATCCGCGAGGACAGCCCGGGCAAGTCGGCGGCGCTGCTGGCGGCGAAAGACCCGGTCAAGGCCCTGCAGCAGCATGAAAAGGCCGGCAAGGCCAGCACGCTGAAg                   <  2:1023169/150‑1 (MQ=255)
                                                                                                                                            cAAGGTGCAGTTGCGCCATATCATGGTCGGCATCATCCGCGAGGACAGCCCGGGCAAGTCGGCGGCGCTGCTGGCGGCGAAAGACCCGGTCAAGGCCCTGCAGCAGCATGAAAAGGCCGGCAAGGCCAGCACGCTGAAGGCACTCGAGCa        <  1:1058671/150‑1 (MQ=255)
                                                                                                                                             aaGGTGCAGTTGCGCCATATCATGGTCGGCATCATCCGCGAGGACAGCCCGGGCAAGTCGGCGGCGCTGCTGGCGGCGAAAGACCCGGTCAAGGCCCTGCAGCAGCATGAAAAGGCCGGCAAGGCCAGCACGCTGAAGGCACTCGAGCAg       <  1:288853/150‑1 (MQ=255)
                                                                                                                                                 tGCAGGTGCGCCATATCATGGTCGGCATCATCCGCGAGGACAGCCCGGGCAAGGCGGCGGCGCTGCTGGCGGCGAAAGACCCGGTCAAGGCCCTGCAGCAGCATGAAAAGGCCGGCAAGGCCAGCACGCTGAAGGCACTCGAGCAGGTGc   >  2:168836/1‑150 (MQ=255)
                                                                                                                                                  gCAGTTGCGCCATATCATGGTCGGCATCATCCGCGAGGACAGCCCGGGCAAGTCGGCGGCGCTGCTGGCGGCGAAAGACCCGGTCAAGGCCCTGCAGCAGCATGAAAAGGCCGGCAAGGCCAGCACGCTGAAGGCACTCGAGCAGGTGcc  >  1:984042/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ATCTGGGCGAAAATGGAAAAAACCGCGTGGATCGCCGACGGCAAGGCCGATGCGCCGCGCAAGGTGTACCTGTTCAGCGACCCGAACTGCCCGTACTGCAACATGTTCTGGGAACAGGCGCGGCCCTGGGTGGAGTCGGGCAAGGTGCAGTTGCGCCATATCATGGTCGGCATCATCCGCGAGGACAGCCCGGGCAAGTCGGCGGCGCTGCTGGCGGCGAAAGACCCGGTCAAGGCCCTGCAGCAGCATGAAAAGGCCGGCAAGGCCAGCACGCTGAAGGCACTCGAGCAGGTGCC  >  NC_002947/4811344‑4811639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑