Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,866,075 T→A 11.6% D169V (GAC→GTC)  PP_4277 ← GntR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,866,0750TA11.6% 115.3 / 7.2 43D169V (GAC→GTC) PP_4277GntR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (18/20);  new base A (2/3);  total (20/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01

CTCTGGCCAGTGCCGCCAGCACCAGTTCGCAGCTGCGCTGGCCATAGGCGGTGAGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCGAACGGATAGGCCGCACGCATGACCGCAAAACTGTCGGCGACGAAGGTCTTGATGTCGAGCTGGTCATGG  >  NC_002947/4865926‑4866221
                                                                                                                                                     |                                                                                                                                                  
ctctGGCCAGTGCCGCCAGCACCAGTTCGCAGCTGCGCTGGCCATAGGCGGTGAGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGt                                                                                                                                                    <  2:582228/150‑1 (MQ=255)
 tctGGCCAGTGCCGCCAGCACCAGTTCGCAGCTGCGCTGGTCATAGGCGGTGAGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTc                                                                                                                                                   <  2:925939/150‑1 (MQ=255)
     gCCAGTGCCGCCAGCACCAGTTCGCAGCTGCGCTGGCCATAGGCGGTGAGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGc                                                                                                                                               >  1:1123282/1‑150 (MQ=255)
     gCCAGTGCCGCCAGCACCAGTTCGCAGCTGCGCTGGCCATAGGCGGTGAGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGc                                                                                                                                               >  1:386888/1‑150 (MQ=255)
         gTGCCGCCAGCACCAGTTCGCAGCTGCGCTGGCCATAGGCGGTGAGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACa                                                                                                                                           <  2:1110955/150‑1 (MQ=255)
          tGCCGCCAGCACCAGTTCGCAGCTGCGCTGGCCATAGGCGGTGAGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGTCGCTGGACCAGCGCCAg                                                                                                                                          <  2:333209/150‑1 (MQ=255)
          tGCCGCCAGCACCAGTTCGCAGCTGCGCTGGCCATAGGCGGTGAGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGTCGCTGGACCAGCGCCAg                                                                                                                                          <  2:1377/150‑1 (MQ=255)
              gccAGCACCAGTTCGCAGCTGCGCTGGCCATAGGCGGTGAGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTaa                                                                                                                                      <  1:896364/150‑1 (MQ=255)
               ccAGCACCAGTTCGCAGCTGCGCTGGCCATAGGCGGTGAGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAAt                                                                                                                                     <  1:35777/150‑1 (MQ=255)
                   cACCAGTTCGCAGCTGCGCTGGCCATAGGCGGTGAGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGc                                                                                                                                 <  1:83807/150‑1 (MQ=255)
                    aCCAGTTCGCAGCTGCGCTGGCCATAGGCGGTGAGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCa                                                                                                                                <  2:214007/150‑1 (MQ=255)
                      cAGTTCGCAGCTGCGCTGGCCATAGGCGGTGAGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACg                                                                                                                              >  2:945071/1‑150 (MQ=255)
                          tCGCAGCTGCGCTGGCCATAGGCGGTGAGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTc                                                                                                                          <  2:584944/150‑1 (MQ=255)
                            gCAGCTGCGCTGGCCATAGGCGGTGAGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTcc                                                                                                                                                  >  1:124861/1‑124 (MQ=255)
                            gCAGCTGCGCTGGCCATAGGCGGTGAGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTcc                                                                                                                                                  <  2:124861/124‑1 (MQ=255)
                                  gcgcTGGCCATAGGCGGTGAGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCgg                                                                                                                  <  2:386888/150‑1 (MQ=255)
                                      tGGCCATAGGCGGTGAGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTCATAGGCACGGCTCACGGCCGGCCGc                                                                                                              >  2:555098/1‑150 (MQ=255)
                                      tGGCCATAGGCGGTGAGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCGTGGCCGGCTGc                                                                                                              >  2:500442/1‑150 (MQ=255)
                                       ggCCATAGGCGGTGAGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCa                                                                                                             <  1:897564/150‑1 (MQ=255)
                                            tAGGCGGTGAGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGttt                                                                                                        >  1:896759/1‑150 (MQ=255)
                                                     aGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGtt                                                                                               >  1:189564/1‑150 (MQ=255)
                                                          cTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAg                                                                                                            <  1:323354/132‑1 (MQ=255)
                                                          cTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAg                                                                                                            >  2:323354/1‑132 (MQ=255)
                                                           tCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGc                                                                                         >  1:750087/1‑150 (MQ=255)
                                                                aaTGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGaa                                                                                    <  2:750087/150‑1 (MQ=255)
                                                                        gCAGGTCACGCGAGACCAGAACTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGt                                                                            <  1:335551/150‑1 (MQ=255)
                                                                             tCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCg                                                                       >  2:762084/1‑150 (MQ=255)
                                                                               aCGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCGaa                                                                     >  2:687944/1‑150 (MQ=255)
                                                                                cgcgAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGCCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCGAAc                                                                    <  1:291666/150‑1 (MQ=255)
                                                                                 gcgAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCGAACg                                                                   >  1:858762/1‑150 (MQ=255)
                                                                                   gagaCCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGACCGCGTTTTCGGCGAACTGa                                                                 <  2:1123282/150‑1 (MQ=255)
                                                                                    agaCCACGGCTTCGAGCAAGCGGGCGAACAGGGCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCGAACGGAt                                                                <  1:841311/150‑1 (MQ=255)
                                                                                                cGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCGAACGGATAGGCcgcacgca                                                    <  1:945071/150‑1 (MQ=255)
                                                                                                         gggCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCGAACGGATAGGCCGCACGCATGACCGCaa                                           >  1:667944/1‑150 (MQ=255)
                                                                                                             gAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACgg                                                                                 <  1:72430/108‑1 (MQ=255)
                                                                                                             gAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACgg                                                                                 >  2:72430/1‑108 (MQ=255)
                                                                                                                cAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCGAACGGATAGGCCGCACGCATGACCGCAAAACTGTc                                    <  1:762084/150‑1 (MQ=255)
                                                                                                                   gTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCGAACGGATAGGCCGCACGCATGACCGCAAAACTGTCGGc                                 >  1:394906/1‑150 (MQ=255)
                                                                                                                       agaTAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCGAACGGATAGGCCGCACGCATGACCGCAAAACTGTCGGcgacg                             >  2:1079879/1‑150 (MQ=255)
                                                                                                                             tCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGATTGTCGGCGAACGGATAGGCCGCACGCATGACCGCAAAACTGTCGGCGACGAAGGTc                       <  2:896759/150‑1 (MQ=255)
                                                                                                                                      attaccTGTCGCTGGACCAGCGCCAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCGAACGGATAGGCCGCACGCATGACCGCAAAACTGTCGGCGACGAAGGTCTTGATGTCg              >  2:425680/5‑150 (MQ=255)
                                                                                                                                          ccTGTCGCTGGACCAGCGCCAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCGAACGGATAGGCCGCACGCATGACCGCAAAACTGTCGGCGACGAAGGTCTTGATGt                <  2:6403/144‑1 (MQ=255)
                                                                                                                                          ccTGTCGCTGGACCAGCGCCAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCGAACGGATAGGCCGCACGCATGACCGCAAAACTGTCGGCGACGAAGGTCTTGATGt                >  1:6403/1‑144 (MQ=255)
                                                                                                                                                  tGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCGAACGGATAGGCCGCACGCATGACCGCAAAACTGTCGGCGACGAAGGTCTTGATGTCGAGCTGGTCATgg  >  1:841891/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CTCTGGCCAGTGCCGCCAGCACCAGTTCGCAGCTGCGCTGGCCATAGGCGGTGAGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCGAACGGATAGGCCGCACGCATGACCGCAAAACTGTCGGCGACGAAGGTCTTGATGTCGAGCTGGTCATGG  >  NC_002947/4865926‑4866221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑