Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,866,082 A→C 14.6% S167A (TCG→GCG)  PP_4277 ← GntR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,866,0820AC14.6% 92.3 / 9.9 41S167A (TCG→GCG) PP_4277GntR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (16/19);  new base C (2/4);  total (18/23)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

GTGCCGCCAGCACCAGTTCGCAGCTGCGCTGGCCATAGGCGGTGAGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCGAACGGATAGGCCGCACGCATGACCGCAAAACTGTCGGCGACGAAGGTCTTGATGTCGAGCTGGTCATGG  >  NC_002947/4865935‑4866221
                                                                                                                                                   |                                                                                                                                           
gTGCCGCCAGCACCAGTTCGCAGCTGCGCTGGCCATAGGCGGTGAGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACa                                                                                                                                           <  2:1110955/150‑1 (MQ=255)
 tGCCGCCAGCACCAGTTCGCAGCTGCGCTGGCCATAGGCGGTGAGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGTCGCTGGACCAGCGCCAg                                                                                                                                          <  2:1377/150‑1 (MQ=255)
 tGCCGCCAGCACCAGTTCGCAGCTGCGCTGGCCATAGGCGGTGAGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGTCGCTGGACCAGCGCCAg                                                                                                                                          <  2:333209/150‑1 (MQ=255)
     gccAGCACCAGTTCGCAGCTGCGCTGGCCATAGGCGGTGAGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTaa                                                                                                                                      <  1:896364/150‑1 (MQ=255)
      ccAGCACCAGTTCGCAGCTGCGCTGGCCATAGGCGGTGAGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAAt                                                                                                                                     <  1:35777/150‑1 (MQ=255)
          cACCAGTTCGCAGCTGCGCTGGCCATAGGCGGTGAGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGc                                                                                                                                 <  1:83807/150‑1 (MQ=255)
           aCCAGTTCGCAGCTGCGCTGGCCATAGGCGGTGAGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCa                                                                                                                                <  2:214007/150‑1 (MQ=255)
             cAGTTCGCAGCTGCGCTGGCCATAGGCGGTGAGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACg                                                                                                                              >  2:945071/1‑150 (MQ=255)
                 tCGCAGCTGCGCTGGCCATAGGCGGTGAGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTc                                                                                                                          <  2:584944/150‑1 (MQ=255)
                         gcgcTGGCCATAGGCGGTGAGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCgg                                                                                                                  <  2:386888/150‑1 (MQ=255)
                             tGGCCATAGGCGGTGAGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTCATAGGCACGGCTCACGGCCGGCCGc                                                                                                              >  2:555098/1‑150 (MQ=255)
                             tGGCCATAGGCGGTGAGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCGTGGCCGGCTGc                                                                                                              >  2:500442/1‑150 (MQ=255)
                              ggCCATAGGCGGTGAGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCa                                                                                                             <  1:897564/150‑1 (MQ=255)
                                   tAGGCGGTGAGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGttt                                                                                                        >  1:896759/1‑150 (MQ=255)
                                            aGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGtt                                                                                               >  1:189564/1‑150 (MQ=255)
                                                 cTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAg                                                                                                            >  2:323354/1‑132 (MQ=255)
                                                 cTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAg                                                                                                            <  1:323354/132‑1 (MQ=255)
                                                  tCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGc                                                                                         >  1:750087/1‑150 (MQ=255)
                                                       aaTGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGaa                                                                                    <  2:750087/150‑1 (MQ=255)
                                                               gCAGGTCACGCGAGACCAGAACTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGt                                                                            <  1:335551/150‑1 (MQ=255)
                                                                    tCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCg                                                                       >  2:762084/1‑150 (MQ=255)
                                                                      aCGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCGaa                                                                     >  2:687944/1‑150 (MQ=255)
                                                                       cgcgAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGCCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCGAAc                                                                    <  1:291666/150‑1 (MQ=255)
                                                                        gcgAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCGAACg                                                                   >  1:858762/1‑150 (MQ=255)
                                                                          gagaCCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGACCGCGTTTTCGGCGAACTGa                                                                 <  2:1123282/150‑1 (MQ=255)
                                                                           agaCCACGGCTTCGAGCAAGCGGGCGAACAGGGCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCGAACGGAt                                                                <  1:841311/150‑1 (MQ=255)
                                                                                       cGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCGAACGGATAGGCcgcacgca                                                    <  1:945071/150‑1 (MQ=255)
                                                                                                gggCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCGAACGGATAGGCCGCACGCATGACCGCaa                                           >  1:667944/1‑150 (MQ=255)
                                                                                                    gAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACgg                                                                                 >  2:72430/1‑108 (MQ=255)
                                                                                                    gAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACgg                                                                                 <  1:72430/108‑1 (MQ=255)
                                                                                                       cAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCGAACGGATAGGCCGCACGCATGACCGCAAAACTGTc                                    <  1:762084/150‑1 (MQ=255)
                                                                                                          gTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCGAACGGATAGGCCGCACGCATGACCGCAAAACTGTCGGc                                 >  1:394906/1‑150 (MQ=255)
                                                                                                              agaTAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCGAACGGATAGGCCGCACGCATGACCGCAAAACTGTCGGcgacg                             >  2:1079879/1‑150 (MQ=255)
                                                                                                                    tCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGATTGTCGGCGAACGGATAGGCCGCACGCATGACCGCAAAACTGTCGGCGACGAAGGTc                       <  2:896759/150‑1 (MQ=255)
                                                                                                                             attaccTGTCGCTGGACCAGCGCCAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCGAACGGATAGGCCGCACGCATGACCGCAAAACTGTCGGCGACGAAGGTCTTGATGTCg              >  2:425680/5‑150 (MQ=255)
                                                                                                                                 ccTGTCGCTGGACCAGCGCCAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCGAACGGATAGGCCGCACGCATGACCGCAAAACTGTCGGCGACGAAGGTCTTGATGt                <  2:6403/144‑1 (MQ=255)
                                                                                                                                 ccTGTCGCTGGACCAGCGCCAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCGAACGGATAGGCCGCACGCATGACCGCAAAACTGTCGGCGACGAAGGTCTTGATGt                >  1:6403/1‑144 (MQ=255)
                                                                                                                                         tggaccAGCGCCAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCGAACGGATAGGCCGCACGCATGACCGCAAAACTGTCGGCGACGAAGGTCTTGATGTCGAGCTGGTCATgg  <  1:425680/146‑1 (MQ=255)
                                                                                                                                         tGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCGAACGGATAGGCCGCACGCATGACCGCAAAACTGTCGGCGACGAAGGTCTTGATGTCGAGCTGGTCATgg  >  1:841891/1‑150 (MQ=255)
                                                                                                                                               aGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCgg                                                                         >  1:891390/1‑73 (MQ=255)
                                                                                                                                               aGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCgg                                                                         <  2:891390/73‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                           
GTGCCGCCAGCACCAGTTCGCAGCTGCGCTGGCCATAGGCGGTGAGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCGAACGGATAGGCCGCACGCATGACCGCAAAACTGTCGGCGACGAAGGTCTTGATGTCGAGCTGGTCATGG  >  NC_002947/4865935‑4866221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑