Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,976,252 A→C 13.1% G188G (GGT→GGG)  flgJ ← peptidoglycan hydrolase FlgJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,976,2520AC13.1% 95.5 / 11.1 46G188G (GGT→GGG) flgJpeptidoglycan hydrolase FlgJ
Reads supporting (aligned to +/- strand):  ref base A (18/22);  new base C (3/3);  total (21/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.21e-01

TCGGCCATTGGCAGCATGGTGGCGACGAAGGCGTCGCTGTCGGTGAAAGCCTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGTTGAGTGCAGCAACGTCGTTGCGCGTAGCGGACGAGGCCATG  >  NC_002947/4976112‑4976399
                                                                                                                                            |                                                                                                                                                   
tCGGCCATTGGCAGCATGGTGGCGACGAAGGCGTCGCTGTCGGTGAAAGCCTTGTTCGGCACAAACGCTGGCTCAGCCACTACAAGGCCCACAATTCGCAGTCCGTTGTCCGGCAGGTCGAAGTCTTTGGCTACTGCCTGACCGTCACgg                                                                                                                                            <  2:680060/150‑1 (MQ=255)
  ggCCATTGGCAGCATGGTGGCGACGAAGGCGGCGATGTCGGTGAAAGCCTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCAGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGggc                                                                                                                                          >  2:287620/1‑150 (MQ=255)
    ccATTGGAAGCAGGGTGGCGACGAAGGCGTCGCTGTCTTAGAAAGCCTTGTTAGCCGGCACCGCTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGggcag                                                                                                                                        <  2:262324/150‑1 (MQ=255)
       ttGGCAGCATGGTGGCGACGAAGGCGTCGCTGTCGGTGAAAGCCTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGggcaggca                                                                                                                                     <  1:381105/150‑1 (MQ=255)
          gcagcaTGGTGGCGACGAAGGCGTCGCTGTCGGTGAAAGCCTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGCCCGTGACGgtcag                                                                                                                                        <  1:1033050/144‑1 (MQ=255)
          gcagcaTGGTGGCGACGAAGGCGTCGCTGTCGGTGAAAGCCTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGCCCGTGACGgtcag                                                                                                                                        >  2:1033050/1‑144 (MQ=255)
            agcaTGGTGGCGACGAAGGCGTCGCTGTCGGTGAAAGCCTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCAcggcg                                                                                                                                >  1:268050/1‑150 (MQ=255)
             gcaTGGTGGCGACGAAGGCGTCGCTGTCGGTGAAAGCCTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCAcggcgg                                                                                                                               <  1:1003164/150‑1 (MQ=255)
                       gacgaAGGCGGCGCTGTCGGTGAGAGCCTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGA‑CGTCACGGGCAGGCACGGCGGCGTTATTGGCa                                                                                                                    <  2:434247/150‑1 (MQ=255)
                          gaAGGCGTCGCTGTCGGTGAAAGGCTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCGGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGCCAGt                                                                                                                  >  2:553720/1‑150 (MQ=255)
                                    cTGTCGGTGAAAGCCTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGCCCGTGACGgtcagg                                                                                                                                       <  2:658624/119‑1 (MQ=255)
                                    cTGTCGGTGAAAGCCTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGCCCGTGACGgtcagg                                                                                                                                       >  1:658624/1‑119 (MQ=255)
                                       tCGGTGAAAGCCTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGc                                                                                                     >  2:463078/1‑150 (MQ=255)
                                          gTGAAAGCCTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACg                                                                                                  >  1:342813/1‑150 (MQ=255)
                                                  cTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGc                                                                                          <  1:1022387/150‑1 (MQ=255)
                                                  cTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCCCGGCTACTGCCTGACCGTAACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGc                                                                                          <  1:1101606/150‑1 (MQ=255)
                                                        cGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAg                                                                                    <  2:322939/150‑1 (MQ=255)
                                                         ggCGCCAGCGGAGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAgg                                                                                   >  2:1123636/1‑150 (MQ=255)
                                                                gCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGcggtcgg                                                                            <  1:17329/150‑1 (MQ=255)
                                                                gCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGcggtcgg                                                                            <  1:17094/150‑1 (MQ=255)
                                                                   gTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTCCCGCCGACGCCACGATAACTGCTAACAGGcggtcgtgga                                                                         <  2:799991/150‑5 (MQ=255)
                                                                           gcaccggcacggcCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCtt                                                                 <  1:188534/146‑1 (MQ=255)
                                                                           gCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCtt                                                                 >  2:909019/1‑150 (MQ=255)
                                                                            ccACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTg                                                                <  1:157966/150‑1 (MQ=255)
                                                                              acggcacggcCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGc                                                              >  2:109923/1‑150 (MQ=255)
                                                                                        cGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGgc                                                    <  1:109923/150‑1 (MQ=255)
                                                                                         gACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGgcg                                                   >  1:91977/1‑150 (MQ=255)
                                                                                         gACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGgcg                                                   >  1:210649/1‑150 (MQ=255)
                                                                                                       cGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAg                                                      >  2:165685/1‑133 (MQ=255)
                                                                                                       cGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAg                                                      <  1:165685/133‑1 (MQ=255)
                                                                                                        gTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGCCCGTGACGgtcagg                                                                                                                                       <  1:1029430/51‑1 (MQ=25)
                                                                                                        gTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGCCCGTGACGgtcagg                                                                                                                                       >  2:1029430/1‑51 (MQ=25)
                                                                                                         ttGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGTTGAGTgca                                   <  1:909019/150‑1 (MQ=255)
                                                                                                              gggCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGTTGAGTGCAGCAAc                              <  2:904131/149‑1 (MQ=255)
                                                                                                                 cACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGTTGAGTGCAGCAAcgtc                           <  1:526659/150‑1 (MQ=255)
                                                                                                                              ttGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGGCGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAAGCGCCGCGCGTTGAGTGCAGCAACGTCGTTGCGCGTAGCg              >  2:280075/1‑150 (MQ=255)
                                                                                                                               gccgccgtgcctgccCGTGACGGTCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGTTGAGTGCAGCAACGTCGTTGCGCGTAGCgg             >  2:806197/8‑150 (MQ=255)
                                                                                                                                  cTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGTTGAGTGCAGCAACGTCGTTGCGCGTAGCGGACg          >  2:216403/1‑150 (MQ=255)
                                                                                                                                  cTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGTTGAGTGCAGCAACGTCGTTGCGCGTAGCGGACg          >  1:190108/1‑150 (MQ=255)
                                                                                                                                   tACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGTTGAGTGCAGTAACGTCGTTGCGCGTAGCGGACGa         >  2:563648/1‑150 (MQ=255)
                                                                                                                                   tACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGTTGAGTGCAGCAACGTCGTTGCGCGTAGCGGACGa         >  2:1120969/1‑150 (MQ=255)
                                                                                                                                        ccTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCa                                                             <  2:968369/93‑1 (MQ=255)
                                                                                                                                        ccTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCa                                                             >  1:968369/1‑93 (MQ=255)
                                                                                                                                          tGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAgg                                                           <  1:1045855/93‑1 (MQ=255)
                                                                                                                                          tGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAgg                                                           >  2:1045855/1‑93 (MQ=255)
                                                                                                                                          tGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCATGGCCAGGCGCCGCGCGTTGAGTGCAGCAACGTCGTTGCGCGTAGCGGACGAGGCCATg  <  2:268050/150‑1 (MQ=255)
                                                                                                                                          tGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGTTGAGTGCAGCAACGTCGTTGCGCGTAGCGGACGAGGCCATg  <  2:465337/150‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                   
TCGGCCATTGGCAGCATGGTGGCGACGAAGGCGTCGCTGTCGGTGAAAGCCTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGTTGAGTGCAGCAACGTCGTTGCGCGTAGCGGACGAGGCCATG  >  NC_002947/4976112‑4976399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑