Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,066,147 G→C 9.5% F164L (TTC→TTG)  PP_4463 ← carbon‑nitrogen hydrolase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,066,1470GC 9.5% 124.0 / 4.7 42F164L (TTC→TTG) PP_4463carbon‑nitrogen hydrolase family protein
Reads supporting (aligned to +/- strand):  ref base G (18/20);  new base C (2/2);  total (20/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

GTTTGACCTGGAGCAAGGAAATAGCACTGCGTTTCAATTGCCCGAGCGCGGCAGAGTACATCCCAATGATCTTTACCAGTTTGAAGGGTAAATGCCGCCGGCAGTACAATTACATCAGCACCCAACGCAACCAGCTTTTGGAAAAGCTCCGGGAAGCGAATGTCGTAGCAGATCGCAAAGCCATACTTAAGGCCTTCAATATCGACCACCGATACCTCTGTACCTGGCGCTACTGCACTGGATTCGCCATAGCGCATACCGTCAGGAGTGACGATGTCGAACAGGTGGAT  >  NC_002947/5066007‑5066296
                                                                                                                                            |                                                                                                                                                     
gTTTGACCTGGAGCAAGGAAATAGCACTGCGTTTCAATTGCCCGAGCGCGGCAGAGTACATCCCAATGATCTTTACCAGTTTGAAGGGTAAATGCCGCCGGCAGTACAATTACATCAGCACCCAACGCAACCAGCTTTTGGAAAAGCTcc                                                                                                                                              >  1:750872/1‑150 (MQ=255)
       cTGGAGCAAGGAAATAGCACTGCGTTTCAATTGCCCGAGCGCGGCAGAGTACATCCCAATGATCTTTACCAGTTTGAAGGGTAAATGCCGCCGGCAGTACAATTACATCAGCACCCAACGCAACCAGCTTTTGGAAAAGCTCCGGGAAGc                                                                                                                                       <  2:380854/150‑1 (MQ=255)
       cTGGAGCAAGGAAATAGCACTGCGTTTCAATTGCCCGAGCGCGGCAGAGTACATCCCAATGATCTTTACCAGTTTGAAGGGTAAATGCCGCCGGCAGTACAATTACATCAGCACCCAACGCAACCAGCTTTTGGAAAAGCTCCGGGAAGc                                                                                                                                       >  1:380854/1‑150 (MQ=255)
       cTGGAGCAAGGAAATAGCACTGCGTTTCAATTGCCCGAGCGCGGCAGAGTACATCCCAATGATCTTTACCAGTTTGAAGGGTAAATGCAGCAGGCAGTACAATTACATCAGCACCCAACGCAACCAGCTTTTGGAAAAGCTCCGGGAAGc                                                                                                                                       >  2:150927/1‑150 (MQ=255)
          gAGCAAGGAAATAGCACTGCGTTTCAATTGCCCGAGCGCGGCAGAGTACATCCCAATGATCTTTACCAGTTTGAAGGGTAAATGCCGCCGGCAGTACAATTACATCAGCACCCAACGCAACCAGCTTTTGGAAAAGCTCCGGGAAGCGaa                                                                                                                                    >  1:572580/1‑150 (MQ=255)
                 gAAATAGCACTGCGTTTCAATTGCCCGAGCGCGGCAGAGTACATCCCAATGATCTTTACCAGTTTGAAGGGTAAATGCCGCCGGCAGTACAATTACATCAGCACCCAACGCAACCAGCTTTTGGAAAAGCTCCGGGAAGCGAATGTCGTa                                                                                                                             <  2:1073431/150‑1 (MQ=255)
                              gTTTCAATTGCCCGAGCGCGGCAGAGTACATCCCAATGATCTTTACCAGTTTGAAGGGTAAATGCCGCCGGCAGTACAATTACATCAGCACCCAACGCAACCAGCTTTTGGAAAAGCTCCGGGAAGCGAATGTCGTAGCAGATCGCAAAg                                                                                                                <  2:750872/150‑1 (MQ=255)
                              gTTTCAATTGCCCAAGCGCGGCAGAGTACATCCCAATGATCTTTACCAGTTTGAAGGGTAAATGCCGCCGGCAGTACAATTACAGCAGCACCCAACGCAACCAGCTTTTGGAAAAGCTCCGGGAAGCGAATGTCGTAGCAGATCGCAAAg                                                                                                                <  1:56692/150‑1 (MQ=255)
                                ttCAATTGCCCGAGCGCGGCAGAGTACATCCCAATGATCTTTACCAGTTTGAAGGGTAAAGGCCGCCGGCAGTACAATTACATCAGCACCCAACGCAACCAGCTTTTGGAAAAGCTCCGGGAAGCGAATGTCGTAGCAGATCGCAAAGcc                                                                                                              <  2:331890/150‑1 (MQ=255)
                                       gCCCGAGCGCGGCAGAGTACATCCCAATGATCTTTACCAGTTTGAAGGGTAAATGCCGCCGGCAGTACAATTACATCAGCACCCAACGCAACCAGCTTTTGGAAAAGCTCCGGGAAGCGAATGTCGTa                                                                                                                             <  2:502939/128‑1 (MQ=255)
                                       gCCCGAGCGCGGCAGAGTACATCCCAATGATCTTTACCAGTTTGAAGGGTAAATGCCGCCGGCAGTACAATTACATCAGCACCCAACGCAACCAGCTTTTGGAAAAGCTCCGGGAAGCGAATGTCGTa                                                                                                                             >  1:502939/1‑128 (MQ=255)
                                        cccGAGCGCGGCAGAGTACATCCCAATGATCTTTACCAGTTTGAAGGGTAAATGCCGCCGGCAGTACAATTACATCAGCACCCAACGCAACCAGCTTTTGGAAAAGCTCCGGGAAGCGAATGTAGTAGCAGATCGCAAAGCCATACTTaa                                                                                                      <  1:176144/150‑1 (MQ=255)
                                           gAGCGCGGCATAGTACATCCCAATGATCTTTACCAGTTTTAAGGGTAAATGCCGCGGGCAGTACAATTACATCAGCACCCAACGCAACCAGCTTTTGGAAAAGCTCCGGGAAGCGAATGTCGTAGCAGATCGCAAAGCCATACTTAAGGc                                                                                                   <  1:303922/150‑1 (MQ=255)
                                                    agagTACATCCCAATGATCTTTACCAGTTTGAAGGGTAAATGCCGCCGGCAGTACAATTACATCAGCACCCAACGCAACCAGCTTTTGGAAAAGCTCCGGGAAGCGAATGTCGTAGCAGATCGc                                                                                                                    <  1:1026474/124‑1 (MQ=255)
                                                    agagTACATCCCAATGATCTTTACCAGTTTGAAGGGTAAATGCCGCCGGCAGTACAATTACATCAGCACCCAACGCAACCAGCTTTTGGAAAAGCTCCGGGAAGCGAATGTCGTAGCAGATCGc                                                                                                                    >  2:1026474/1‑124 (MQ=255)
                                                            tCCCAATGATCTTTACCAGTTTGAAGGGTAAATGCCGCCGGCAGTACAATTACATCAGCACCCAACGCAACCAGCTTTTGGAAAAGCTCCGGGAAGCGAATGTCGTAGCAGATCGCAAAGCCATACTTAAGGCCTTCAATATCGaccacc                                                                                  >  1:740440/1‑150 (MQ=255)
                                                             cccAATGATCTTTACCAGTTTGAAGGGTAAATGCCGCCGGCAGTACAATTACATCAGCACCCAACGCAACCAGCTTTTGGAAAAGCTCCGGGAAGCGAATGTCGTAGCAGATCGCAAAGCCATACTTAAGGCCTTCAATATCGACCACCg                                                                                 >  1:463612/1‑150 (MQ=255)
                                                               cAATGATCTTTACCAGTTTGAAGGGTAAATGCCGCCGGCAGTACAATTACATCAGCACCCAACGCAACCAGCTTTTGGAAAAGCTCCGGGAAGCGAATGTCGTAGCAGATCGCAAAGCCATACTTAAGGCCTTCAATATCGACCACCGAt                                                                               >  1:361219/1‑150 (MQ=255)
                                                                aaggATCTTTACCAGTTTGAAGGGTAAATGCCGCCGGCAGTACAATTACATCAGCACCCAACTCAACCAGCTTTTTGAATAGCTCCTGGAAGCGAATGTCGTAGCAGCTCGCAAAGCCATACTTAAGGCCTTCAATATCGACCACCGATa                                                                              <  2:89913/147‑1 (MQ=255)
                                                                aaTGATCTTTACCAGTTTGAAGGGTAAATGCCGCCGGCAGTACAATTACATCAGCACCCAACGCAACCAGCTTTTGGAAAAGCTCCGGGAAGCGAATGTCGTAGCAGATCGCAAAGCCATACTTAAGGCCTTCAATATCGACCACCGATa                                                                              >  1:185525/1‑150 (MQ=255)
                                                                           ccAGTTTGAAGGGTAAATGCCGCCGGCAGTACAATTACATCAGCACCCAACGCAACCAGCTTTTGGAAAAGCTCCGGGAATCGAATGTCGTAGCAGATCGCACAGCCATACTTAAGGCCTTCAATATCGACCACCGATACCTCTGTACCt                                                                   <  2:572580/150‑1 (MQ=255)
                                                                            aaGTTTGAAGGGTAAATGCCGCCGGCAGTACAATTACATCAGCACCCAACGCAACCAGCTTTTGGAAAAGCTCCGGGAAGCGAATGTCGTAGCAGATCGCAAAGCCATACTTAAGGCCTTCAATATCGACCACCGATACCTCTGTACCTg                                                                  >  1:936547/2‑150 (MQ=255)
                                                                                        tAAATGCCGCCGGCAGTACAATTACATCAGCACCCAACGCAACCAGCTTTTGGAAAAGCTCCGGGAAGCGAATGTCGTAGCAGATCGCAAAGCCATACTTAAGGCCTTCAATATCGACCACAGTTATCTCTGTACCTGGCGCTACTGCAc                                                      <  1:150927/150‑1 (MQ=255)
                                                                                                          cAATTACATCAGCACCAAACGCAACCAGCTTTTGGAAAAGCTCCGGGAAGCGAATGTCGTAGCAGATCGCAAAGCCATACTTAAGGCCTTCAATATCGACCACCGATACCTCTGTACCTGGCGCTACTGCACTGGATTCGCCATAGCGCa                                    <  2:626654/150‑1 (MQ=255)
                                                                                                                cATCAGCACCCAACGCAACCAGCTTTTGGAAAAGCTCCGGGAAGCGAATGTCGTAGCAGATCGCAAAGCCATACTTAAGGCCTTCAATATCGACCACCGATACCTCTGTACCTGGCGCTACTGCACTGGATTCGCCATAGCGCATACCGt                              <  2:936547/150‑1 (MQ=255)
                                                                                                                 aTCAGCACCCAACGCAACCAGCTTTTGGAAAAGCTCCGGGAAGCGAATGTCGTAGCAGATCGCAAAGCCATACTTAAGGCCTTCAATATCGACCACCGATACCTCTGTACCTg                                                                  <  1:1086971/113‑1 (MQ=255)
                                                                                                                 aTCAGCACCCAACGCAACCAGCTTTTGGAAAAGCTCCGGGAAGCGAATGTCGTAGCAGATCGCAAAGCCATACTTAAGGCCTTCAATATCGACCACCGATACCTCTGTACCTg                                                                  >  2:1086971/1‑113 (MQ=255)
                                                                                                                 aTCAGCACCCAACGCAACCAGCTTTTGGAAAAGCTCCGGGAAGCGAATGTCGTAGCAGATCGCAAAGCCATACTTAAGGCCTTCAATATCGACCACCGATACCTCTGTACCTGGCGCTACTGCACTGGATTCGCCATAGCGCATACCGTc                             >  1:287866/1‑150 (MQ=255)
                                                                                                                 aTCAGCACCCAACGCAACCAGCTTTTGGAAAAGCTCCGGGAAGCGAATGTCGTAGCAGATCGCAAAGCCATACTTAAGGCCTTCAATATCGACCACCGATACCTCTGTACCTGGCGCTACTGCACTGGATTCGCCATAGCGCATACCGTc                             >  1:822526/1‑150 (MQ=255)
                                                                                                                  tCAGCACCCAACGCAACCAGCTTTTGGAAAAGCTCCGGGAAGCGAATGTCGTAGCAGATCGCAAAGCCATACTTAAGGCCTTCAATATCGACCACCGATACCTCTGTACCTGGCGCTACTGCACTGGATTc                                               <  2:873425/131‑1 (MQ=255)
                                                                                                                  tCAGCACCCAACGCAACCAGCTTTTGGAAAAGCTCCGGGAAGCGAATGTCGTAGCAGATCGCAAAGCCATACTTAAGGCCTTCAATATCGACCACCGATACCTCTGTACCTGGCGCTACTGCACTGGATTc                                               >  1:873425/1‑131 (MQ=255)
                                                                                                                         ccAACGCAACCAGCTTTTGGAAAAGCTCCGGGAAGCGAATGTCGTAGCAGATCGCAAAGCCATACTTAAGGCCTTCAATATCGACCACCGATACCTCTGTACCTGGCGCTACTGCACTGGATTCGCCATAGCGCATACCGTCAGGAGTGa                     <  2:463612/150‑1 (MQ=255)
                                                                                                                           aaCGCAACCAGCTTTTGGAAAAGCTCCGGGAAGCGAAt                                                                                                                                   >  2:444500/1‑38 (MQ=255)
                                                                                                                           aaCGCAACCAGCTTTTGGAAAAGCTCCGGGAAGCGAAt                                                                                                                                   <  1:444500/38‑1 (MQ=255)
                                                                                                                           aaCGCAACCAGCTTTTGGAAAAGCTCCGGGAAGCGAATGTCGTAGCAGATCGCAAAGCCATACTTAAGGCCTTCAATATCGACCACCGATACCTCTGTACCTGGCGCTACTGCACTGGATTCGCCATAGCGCATACCGTCAGGAGTGACg                   >  2:772121/1‑150 (MQ=255)
                                                                                                                                ccggaGCTTTTCCAAAAGCTCCGGGAAGCGAATGTCGTAGCAGATCGCAAAGCCATACTTAAGGCCTTCAATATCGACCACCGATACCTCTGTACCTGGCGCTACTGCACTGGATTCGCCATAGCGCATACCGTCAGGAGTGAc                    <  1:104953/140‑1 (MQ=255)
                                                                                                                                ccggaGCTTTTCCAAAAGCTCCGGGAAGCGAATGTCGTAGCAGATCGCAAAGCCATACTTAAGGCCTTCAATATCGACCACCGATACCTCTGTACCTGGCGCTACTGCACTGGATTCGCCATAGCGCATACCGTCAGGAGTGAc                    >  2:104953/5‑144 (MQ=255)
                                                                                                                                aaCCAGCTTTTGGAAAAGCTCCGGGAAGCGAATGTCGTAGCAGATCGCAAAGCCATACTTAAGGCCTTCAATATCGACCACCGATACCTCTGTACCTGGCGCTACTGCACTGGATTCGCCATAGCGCATACCGTCAGGAGTGACGATGTc              <  2:361219/150‑1 (MQ=255)
                                                                                                                                aaCCAGCTTTTGGAAAAGCTCCGGGAAGCGAATGTCGTAGCAGATCGCAAAGCCATACTTAAGGCCTTCAATATCGACCACCGATACCTCTGTACCTGGCGCTACTGAACTGGATTCGCCATAGCGCATACCATCAGGCGTGACGATGTc              >  2:219337/1‑150 (MQ=255)
                                                                                                                                 cggaGCTTTTCCAAAAGCTCCGGGAAGCGAATGTCGTAGCAGATCGCAAAGCCATACTTAAGGCCTTCAATATCGaccac                                                                                   >  1:996502/4‑80 (MQ=255)
                                                                                                                                 cggaGCTTTTCCAAAAGCTCCGGGAAGCGAATGTCGTAGCAGATCGCAAAGCCATACTTAAGGCCTTCAATATCGaccac                                                                                   <  2:996502/77‑1 (MQ=255)
                                                                                                                                   cAGCTTTTGGAAAAGCTCCGGGAAGCGAATGTCGTAGCAGATCGCAAAGCCATACTTAAGGCCTTCAATATCGACCACCGATACCTCTGTACCTGGCGCTACTGCACTGGATTCGCCATAGCGCATACCGTCAGGAGTGACGATGTCGaa           <  2:940462/150‑1 (MQ=255)
                                                                                                                                            gAAAAGCTCCGGGAAGCGAATGTCGTAGCAGATCGCAAAGCCATACTTAAGGCCTTCGATATCGACCACCGATACCTCTGTACCTGGCGCTACTGCACTGGATTCGCCATAGCGCATACCGTCAGGAGTGACGATGTCGAACAGGTGGAt  >  2:924596/1‑150 (MQ=255)
                                                                                                                                            |                                                                                                                                                     
GTTTGACCTGGAGCAAGGAAATAGCACTGCGTTTCAATTGCCCGAGCGCGGCAGAGTACATCCCAATGATCTTTACCAGTTTGAAGGGTAAATGCCGCCGGCAGTACAATTACATCAGCACCCAACGCAACCAGCTTTTGGAAAAGCTCCGGGAAGCGAATGTCGTAGCAGATCGCAAAGCCATACTTAAGGCCTTCAATATCGACCACCGATACCTCTGTACCTGGCGCTACTGCACTGGATTCGCCATAGCGCATACCGTCAGGAGTGACGATGTCGAACAGGTGGAT  >  NC_002947/5066007‑5066296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑