Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,080,327 A→T 9.8% intergenic (‑144/+24) csrA ← / ← lysC carbon storage regulator/aspartokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,080,3270AT 9.8% 115.6 / 5.0 41intergenic (‑144/+24)csrA/lysCcarbon storage regulator/aspartokinase
Reads supporting (aligned to +/- strand):  ref base A (20/17);  new base T (2/2);  total (22/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.28e-01

CTTTCTCCTTACGCAAAACATTCAGGGGTAACAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTACTTCTCTTCGAGCACCACCGAAACCTTGATCTCGGAAGTGGAGATCAT  >  NC_002947/5080186‑5080470
                                                                                                                                             |                                                                                                                                               
cTTTCTCCTTACGCAAAACATTCAGGGGTAACAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCTTGCCGCGcc                                                                                                                                         <  1:887009/150‑1 (MQ=255)
       cTTACGCAAAACATTCAGGGGTAACAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAg                                                                                                                                  >  2:928856/1‑150 (MQ=255)
            gCAAAACATTCAGGGGTAACAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAgc                                                                                                                             >  2:831831/1‑150 (MQ=255)
            gCAAAACATTCAGGGGTAACAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAACGGCGCGGCAAGCCGCGCCCTTCCAGGCAgc                                                                                                                             >  1:45344/1‑150 (MQ=255)
             cAAAACATTCAGGGGTAACAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGAAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAgcg                                                                                                                            >  1:1086057/1‑150 (MQ=255)
              aaaaCATTCAGGGGTAACAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAgcgc                                                                                                                           >  2:462463/1‑150 (MQ=255)
               aaaCATGTAGGGGTAACAGTCGTGGATATCGGGCCCGGGTCGCGGGAGACGACGATGGCCCCGGTTACTCGCAGCACAAGGCAGGCCAAGACCTGCGCGGCGAGCAGAAAGGCGGAGGGCGGGGCAAGCCGAGCCCTTCCGGGCAgcgtc                                                                                                                          >  2:401569/1‑148 (MQ=255)
                aaCATTCAGGGGTAACAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCt                                                                                                                         >  2:1072337/1‑150 (MQ=255)
                       aGGTGTAACAGTCTTGGATATCGGGCCCGGGTCGCGGACCACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGc                                                                                                                  <  2:653270/150‑1 (MQ=255)
                        ggggTAACAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGcc                                                                                                                 <  1:772454/150‑1 (MQ=255)
                            tAACAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGt                                                                                                             >  1:687493/1‑150 (MQ=255)
                             aaCAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTc                                                                                                            <  2:635004/150‑1 (MQ=255)
                               cAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACACGGCAGGACAGGACCTGCGTGGCGAGCAGAAGCGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCgg                                                                                                          <  1:631289/150‑1 (MQ=255)
                               cAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCgg                                                                                                          <  1:338705/150‑1 (MQ=255)
                                aGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCggg                                                                                                         >  2:880620/1‑150 (MQ=255)
                                     tGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGa                                                                                                    <  1:590072/150‑1 (MQ=255)
                                           tCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATcc                                                                                              <  2:687493/150‑1 (MQ=255)
                                             gggCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAg                                                                                            >  1:204093/1‑150 (MQ=255)
                                               gCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGAt                                                                                          <  1:1072337/150‑1 (MQ=255)
                                               gCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGAt                                                                                          <  1:223114/150‑1 (MQ=255)
                                                cccGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAAAAACGCGAAGGGCGCGGCTTGCCGCGCCCTTc                                                                                                                                     <  2:675428/106‑1 (MQ=255)
                                                cccGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAAAAACGCGAAGGGCGCGGCTTGCCGCGCCCTTc                                                                                                                                     >  1:675428/1‑106 (MQ=255)
                                                      tCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGCTCGAAcgc                                                                                   >  2:440335/1‑150 (MQ=255)
                                                          ggACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCa                                                                                                       <  1:275043/126‑1 (MQ=255)
                                                          ggACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCa                                                                                                       >  2:275043/1‑126 (MQ=255)
                                                              gacgacCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCa                                                                           >  1:423975/1‑150 (MQ=255)
                                                                                     gccgcACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATc                                                                                               <  1:889164/107‑1 (MQ=255)
                                                                                     gccgcACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATc                                                                                               >  2:889164/1‑107 (MQ=255)
                                                                                        gcACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTAc                                                 <  1:928856/150‑1 (MQ=255)
                                                                                                    tcctgtccTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTACTTCTCTTCGAGc                                     >  1:940394/7‑150 (MQ=255)
                                                                                                               gcggcgAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAAc                                                                                     >  1:663273/1‑91 (MQ=255)
                                                                                                               gcggcgAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAAc                                                                                     <  2:663273/91‑1 (MQ=255)
                                                                                                               gcggcgAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCAGCCAGATCGAACGCGGTATGCAAAGAGCGTATCGAGAGCGGCAGGGACTTCTCTTCGAGCACCACCGAAAc                          >  1:247360/1‑150 (MQ=255)
                                                                                                                        aGAAACGCGAAGGACGCGGCAAGCCGCGCCCTTCCAGTCAGCTCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGAACGACCAGCCCCAGGCACTTCTCCTCAAGCACCACCGAAACCTTGAtctc                 >  2:514737/1‑150 (MQ=255)
                                                                                                                          aaaCGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTACTTCTCTTCGAGCACCACCGAAACCTTGATCTCgg               <  1:515058/150‑1 (MQ=255)
                                                                                                                          aaaCGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTACTTCTCTTCGAGCACCACCGAAACCTTGATCTCgg               <  1:514737/150‑1 (MQ=255)
                                                                                                                            aCGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTACTTCTCTTCGAGCACCACCGAAACCTTGATCTCGGaa             <  1:940496/150‑1 (MQ=255)
                                                                                                                                gAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTACTTCTCTTCGAGCACCACCGAAACCTTGATCTCGGAAGTgg         >  2:526425/1‑150 (MQ=255)
                                                                                                                                   gggCGCGGCTTGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTACTTCTCTTCGAGCACCACCGAAACCTTGATCTCGGAAGTGgaga      >  1:317072/1‑150 (MQ=255)
                                                                                                                                      cgcgGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTACTTCTCTTCGAGCACCACCGAAACCTTGATCTCGGAAGTGGAGatca   >  2:364407/1‑150 (MQ=255)
                                                                                                                                       gcgGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTACTTCTCTTCGAGCACCACCGAAACCTTGATCTCGGAAGTGGAGatcat  <  1:364407/150‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                               
CTTTCTCCTTACGCAAAACATTCAGGGGTAACAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTACTTCTCTTCGAGCACCACCGAAACCTTGATCTCGGAAGTGGAGATCAT  >  NC_002947/5080186‑5080470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑