| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 461,033 | C→G | 9.3% | V479L (GTG→CTG) | pqqF ← | coenzyme PQQ synthesis protein F |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 461,033 | 0 | C | G | 9.3% | 163.9 / 7.1 | 54 | V479L (GTG→CTG) | pqqF | coenzyme PQQ synthesis protein F |
| Reads supporting (aligned to +/- strand): ref base C (26/23); new base G (2/3); total (28/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.63e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
GCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTG‑GCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACGGCTGGCGCATAG > NC_002947/460889‑461173 | gCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTG‑GCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCActgctg > 2:231472/1‑150 (MQ=255) gCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGTAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTAGCAGGTC‑GCTTAAGCATCAAGGCCACGGCACGTGCAACTGCCCGCAGCTCTACGGCTgg > 2:512820/1‑150 (MQ=255) gCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTG‑GCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTgg > 2:805170/1‑150 (MQ=255) gCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTG‑GCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTgg < 1:814799/150‑1 (MQ=255) tACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTG‑GCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCAGCAGCAGTGCTGCGGGCa < 1:982431/150‑1 (MQ=255) aCCCCGTACCGCGTCTGGCAGTTGTTTGATCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTG‑GCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAg < 1:280463/150‑1 (MQ=255) cccGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTG‑GCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGc > 1:494652/1‑150 (MQ=255) ccGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTG‑GCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCActgc > 1:703336/1‑138 (MQ=255) ccGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTG‑GCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCActgc < 2:703336/138‑1 (MQ=255) cgcgTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTG‑GCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCa < 1:823392/140‑1 (MQ=255) cgcgTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTG‑GCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCa > 2:823392/1‑140 (MQ=255) gCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTG‑GCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCTCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACt < 2:595243/150‑1 (MQ=255) gCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTG‑GCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACt < 2:249697/150‑1 (MQ=255) ttGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTG‑GCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCa > 2:744348/1‑134 (MQ=255) ttGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTG‑GCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCa < 1:744348/134‑1 (MQ=255) ttGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTG‑GCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTAt > 1:226847/1‑150 (MQ=255) ttGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTG‑GCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTAt > 2:424171/1‑150 (MQ=255) gAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTG‑GCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTc < 1:244315/150‑1 (MQ=255) aGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTG‑GCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTcc < 1:131791/150‑1 (MQ=255) gCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTG‑GCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTccc < 2:30525/150‑1 (MQ=255) ggCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTG‑GCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACt > 2:129404/1‑134 (MQ=255) ggCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTG‑GCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACt < 1:129404/134‑1 (MQ=255) gCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGTAACTGGCAGGTG‑GCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAg > 2:343913/1‑116 (MQ=255) gCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGTAACTGGCAGGTG‑GCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAg < 1:343913/116‑1 (MQ=255) gCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTG‑GCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCa > 1:267766/1‑150 (MQ=255) ccTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTG‑GCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCAct > 1:873599/1‑150 (MQ=255) tGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTG‑GCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCActgc > 2:253969/1‑150 (MQ=255) gggCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTG‑GCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGtt > 1:865083/1‑150 (MQ=255) gggCAGCGCCGTGCAGGGCAGCCAGCACCTGGACGGTGCGGGTGA‑TAGTAAAGCCAAGGCTTGTTCCACTGCCAGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGa < 1:805170/150‑1 (MQ=255) cgcCGTGCAGGGCAGCCAGCAACTGGCAGGTG‑GCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGcgcg < 2:654579/150‑1 (MQ=255) gcCGTGCAGGGCAGCCAGCAACTGGCAGGTG‑GCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCgg < 2:634740/150‑1 (MQ=255) gcCGTGCAGGGCAGCCAGCAACTGGCAGGTG‑GCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCgg < 2:226847/150‑1 (MQ=255) ccGTGCAGGGCAGCCAGCAACTGGCAGGTG‑GCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGt < 2:865083/150‑1 (MQ=255) agccagcAACTGGCAGGTG‑GCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTcc > 1:650155/1‑150 (MQ=255) gccagcAACTGGCAGGTG‑GCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCt < 1:254902/150‑1 (MQ=255) ccagcAACTGGCAGGTG‑GCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGAGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTg > 1:685500/1‑150 (MQ=255) gTG‑GCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAggg > 2:1030835/1‑150 (MQ=255) gg‑gCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGc < 2:725316/149‑1 (MQ=255) gCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACGGCTGTACTGCAGTTGCACGGAGGCGCGGTGACAACGTTCCTGCAGTGGCGCCAGGGCCt < 2:442427/150‑1 (MQ=255) ttGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGt < 2:753938/150‑1 (MQ=255) gCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGATCTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTGCCa > 1:912149/1‑150 (MQ=255) gCATAACGGCCAAGGCTTGTGGCTGTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCa > 2:272070/1‑150 (MQ=255) aTCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGc > 1:558849/1‑150 (MQ=255) ccaaggcTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCt > 1:349124/1‑150 (MQ=255) caaggcTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCa < 1:626869/92‑1 (MQ=255) caaggcTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCa > 2:626869/1‑92 (MQ=255) caaggcTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGgc > 2:3098/1‑99 (MQ=255) caaggcTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGgc < 1:3098/99‑1 (MQ=255) cggccTGCCAGCAGCAGTGCTGCGGGCAGTCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCgg > 2:460789/5‑93 (MQ=37) cggccTGCCAGCAGCAGTGCTGCGGGCAGTCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGGTGCACGGAGGCGCgg < 1:460789/89‑1 (MQ=37) cACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACGGCTGgcg > 1:32817/1‑150 (MQ=255) cTGCCAGCAGCAGTGCTGCGGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACaa > 2:120055/1‑95 (MQ=39) cTGCCAGCAGCAGTGCTGCGGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACaa < 1:120055/95‑1 (MQ=39) tGCCCGCAGCACTGCTGCTGGCAGGTCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACGGCTGGCGCAt > 1:160875/1‑150 (MQ=255) cccGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACGGCTGGCGCATAg > 1:486760/1‑150 (MQ=255) cccGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACGGCTGGCGCATAg > 1:213151/1‑150 (MQ=255) cccGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACATTCCTGCAGGGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACGGCTGGCGCATAg < 2:830597/150‑1 (MQ=255) | GCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTG‑GCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACGGCTGGCGCATAG > NC_002947/460889‑461173 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |