Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,390,631 C→A 10.8% intergenic (+672/‑50) PP_4738 → / → PP_mr56 hypothetical protein/C4 ncRNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,390,6310CA10.8% 117.0 / 3.2 44intergenic (+672/‑50)PP_4738/PP_mr56hypothetical protein/C4 ncRNA
Reads supporting (aligned to +/- strand):  ref base C (20/19);  new base A (2/3);  total (22/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTG  >  NC_002947/5390483‑5390776
                                                                                                                                                    |                                                                                                                                                 
gCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCt                                                                                                                                                  <  1:225275/150‑1 (MQ=255)
 cGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTGTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCtc                                                                                                                                                 <  1:685196/150‑1 (MQ=255)
  gTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCtca                                                                                                                                                >  1:751964/1‑150 (MQ=255)
   tCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCtcat                                                                                                                                               <  2:751005/150‑1 (MQ=255)
          ttGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGATGAGCAACGAc                                                                                                                                        <  2:522800/150‑1 (MQ=255)
          ttGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGATGAGCAACGAc                                                                                                                                        <  1:682908/150‑1 (MQ=255)
                 tGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCttt                                                                                                                                 <  2:1009744/150‑1 (MQ=255)
                        tGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCACCAACGACCGGCTTTGACNGGc                                                                                                                          <  2:696419/150‑1 (MQ=255)
                             gcTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCaa                                                                                                                     <  1:637764/150‑1 (MQ=255)
                                        aaaaaTCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAgc                                                                                                          <  1:325770/150‑1 (MQ=255)
                                                      aCCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTc                                                                                            >  2:731526/1‑150 (MQ=255)
                                                       ccccTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCa                                                                                           >  2:51442/1‑150 (MQ=255)
                                                        cccTTTCTTGTAGGGCGTTTCCCAAAGATGGTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCAt                                                                                          >  1:30203/1‑150 (MQ=255)
                                                        cccTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCAt                                                                                          >  2:638426/1‑150 (MQ=255)
                                                        cccTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCAt                                                                                          >  1:902614/1‑150 (MQ=255)
                                                         ccgatCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCA‑TTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATgg                                                                                        <  2:193005/146‑1 (MQ=255)
                                                          cTTTCCTGTTGGGCGTTTCCCAAAAATACTGTCTGCTTCTGTTATGCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCCCCGATCGCCTTTGACAGGCCGCACCGATTCCCAGCGCGCAGAACCGtgtattt                                                                                        <  2:346182/150‑7 (MQ=255)
                                                               ttGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTTATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATaa                                                                                                  <  2:571104/135‑1 (MQ=255)
                                                               ttGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATaa                                                                                                  >  1:571104/1‑135 (MQ=255)
                                                                 gTAGGGCGTTTCCCAAAGATACTGCCTGCGCATGTTTTAAGTTGTGGTC‑TTAATGGAGGGTTTTAGTCTCGGTGGTTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGcagatcgg                                                                                >  1:193005/1‑143 (MQ=255)
                                                                   aGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAAGGTAATAGACCCGGACGGTGATTTCTCATCTTCGATCGTCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGc                                                                               <  1:689084/150‑1 (MQ=255)
                                                                             ccAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGAt                                                                                                                 <  2:336341/106‑1 (MQ=255)
                                                                             ccAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGAt                                                                                                                 >  1:336341/1‑106 (MQ=255)
                                                                               aaaGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGCGCACgaa                                                                   >  2:1082516/1‑148 (MQ=255)
                                                                                aaGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGCGCACGcaa                                                                  <  1:1082516/150‑3 (MQ=255)
                                                                                          ctgcGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCg                                                        >  2:1017869/1‑150 (MQ=255)
                                                                                             cgcCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGtt                                                     >  1:329547/1‑150 (MQ=255)
                                                                                              gcCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCttt                                                                                                                                 >  2:786912/1‑73 (MQ=255)
                                                                                              gcCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCttt                                                                                                                                 <  1:786912/73‑1 (MQ=255)
                                                                                                cTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCtt                                                                  >  2:325259/1‑134 (MQ=255)
                                                                                                cTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCtt                                                                  <  1:325259/134‑1 (MQ=255)
                                                                                                   ttttCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCtgtg                                               >  1:431569/1‑150 (MQ=255)
                                                                                                          ttGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCg                                        >  2:848482/1‑150 (MQ=255)
                                                                                                           tGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCgg                                       <  2:751964/150‑1 (MQ=255)
                                                                                                            gCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGt                                      >  1:416144/1‑150 (MQ=255)
                                                                                                                tCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCAt                                                                                          <  2:200346/94‑1 (MQ=255)
                                                                                                                tCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCAt                                                                                          >  1:200346/1‑94 (MQ=255)
                                                                                                                tCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGt                                  <  1:848482/150‑1 (MQ=255)
                                                                                                                   ttttttCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATTAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCaa                               >  2:925355/1‑150 (MQ=255)
                                                                                                                                    tctcGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATAGACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGcca              <  2:516749/150‑1 (MQ=255)
                                                                                                                                            ggTCGTTGATGAGCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCt                                    <  1:1106587/120‑1 (MQ=255)
                                                                                                                                            ggTCGTTGATGAGCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCt                                    >  2:1106587/1‑120 (MQ=255)
                                                                                                                                             gTCGTTGATGAGCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGt     >  1:213911/1‑150 (MQ=255)
                                                                                                                                                gTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTg  >  2:976873/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTG  >  NC_002947/5390483‑5390776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑