| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,456,653 | A→T | 8.3% | K113* (AAG→TAG) ‡ | leuS → | leucine‑‑tRNA ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,456,653 | 0 | A | T | 8.3% | 142.7 / 3.9 | 48 | K113* (AAG→TAG) ‡ | leuS | leucine‑‑tRNA ligase |
| Reads supporting (aligned to +/- strand): ref base A (26/18); new base T (2/2); total (28/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01 | |||||||||||
CCGCTACCAGCGCATGCTGGGCAAGAACGTCCTGCAGCCGATGGGCTGGGACGCTTTCGGCATGCCCGCGGAAAACGCGGCGATGAAAAACAACGTCGCCCCGGCCAAGTGGACGTACGAAAACATCGACTACATGAAGACCCAG‑CTCAAGAGCCTGGGCCTGGCCATCGACTGGGCACGTGAAGTCACCACCTGCAAACCCGACTATTACCGTTGGGAGCAGTGGCTGTTCACCCGCCTGTTCGAAAAAGGCATCATCTACCGCAAGAACGGTACCGTGAACTGGGACCCGGCGGAC > NC_002947/5456505‑5456802 | ccGCTACCAGCGCATGCTGGGCAAGAACGTCCTGCAGCCGATGGGCTGGGACGCTTTCGGCATGCCCGCGGAAAACGCGGCGATGAAAAACAACGTCGCCCCGGCCAAGTGGACGTACGAAAACATCGACTACATGAAGACCCAG‑CTCaa > 1:270314/1‑150 (MQ=255)ccGCTACCAGCGCATGCTGGGCAAGAACGTCCTGCAGCCGATGGGCTGGGACGCTTTCGGCATGCCCGCGGAAAACGCGGCGATGAAAAACAACGTCGCCCCGGCCAAGTGGACGTACGAAAACATCGACTACATGAAGACCCAG‑CTCaa > 1:375375/1‑150 (MQ=255) cAGCGCATGCTGGGCAAGAACGTCCTGCAGCCGATGGGCTGGGACGCTTTCGGCATGCCCGCGGAAAACGCGGCGATGAAAAACAACGTCGCCCCGGCCAAGTGGACGTACGAAAACATCGACTACATGAAGACCCAG‑CTCAAGAgcctg > 1:939154/1‑150 (MQ=255) gcgcATGCTGGGCAAGAACGTCCTGCAGCCGATGGGCTGGGACGCTTTCGGCATGCCCGCGGAAAACGCGGCGATGAAAAACAACGTCGCCCCGGCCAAGTGGACGTACGAAAACATCGACTACATGAAGACCCAG‑CTCAAGAgcctggg > 2:146419/1‑150 (MQ=255) tgacaggCAACAACAGCCTACAGCCGATGGGCTGGGACGCTTTCGGCATGCCCGCGGAAAACGCGGCGATGAAAAACAACGTCGCCCCGGCCAAGTGGACGTACGAAAACATCGACTACATGAAGACCCAG‑CTCAAGAgcctgggcctgg > 2:103276/6‑150 (MQ=255) ggCAAGAACGTCCTGCAGCCGATGGGCTGGGACGCTTTCGGCATGCCCGCGGAAAACGCGGCGATGAAAAACAACGTCGCCCCGGCCAAGTGGACGTACGAAAACATCGACTACATGAAGACCCAG‑CTCAAGAGCCTGGGCCTGGCCATc < 2:1036393/150‑1 (MQ=255) aaGAACGTCCTGCAGCCGATGGGCTGGGACGCTTTCGGCATGCCCGCGGAAAACGCGGCGATGAAAAACAACGTCGCCCCGGCCAAGTGGACGTACGAAAACATCGACTACATGAAGACCCAG‑CTCAAGAGCCTGGGCCTGGCCATCGAc > 2:837741/1‑150 (MQ=255) aaGAACGTCCTGCAGCCGATGGGCTGGGACGCTTTCGGCATGCCCGCGGAAAACGCGGCGACGAAAAACAACGTCGCCCCGGCCAAGTGGACGTACGAAAACATCGACTACATGAAGACCCAG‑CTCAAAAGCCTGGGCCTGGCCATCGAc > 2:384355/1‑150 (MQ=255) cTGCAGCCGATGGGCTGGGACGCTTTCGGCATGCCCGCGGAAAACGCGGCGATGAAAAACAACGTCGCCCCGGCCAAGTGGACGTACGAAAACATCGACTACATGAAGACCCAG‑CTCAAGAGCCTGGGCCTGGCCATCGACTGGGCACgg > 1:677212/1‑149 (MQ=255) cTGCAGCCGATGGGCTGGGACGCTTTCGGCATGCCCGCGGAAAACGCGGCGATGAAAAACAACGTCGCCCCGGCCAAGTGGACGTACGAAAACATCGACTACATGAAGACCCAG‑CTCAAGAGCCTGGGCCTGGCCATCGACTGGGCACGt < 2:947034/150‑1 (MQ=255) cAGCCGATGGGCTGGGACGCTTTCGGCATGCCCGCGGAAAACGCGGCGATGAAAAACAACGTCGCCCCGGCCAAGTGGACGTACGAAAACATCGACTACATGAAGACCCAG‑CTCAAGAGCCTGGGCCTGGCCATCGACTGGGCACGTGaa < 2:375375/150‑1 (MQ=255) gATGGGCTGGGACGCTTTCGGCATGCCCGCGGAAAACGCGGCGATGAAAAACAACGTCGCCCCGGCCAAGTGGACGTACGAAAACATCGACTACATGAAGACCCAG‑CTCAAGAGCCTGGGCCTGGCCATCGACTGGGCACGTGAAGTcac > 2:1076143/1‑150 (MQ=255) gCTGGGACGCTTTCGGCATGCCCGCGGAAAACGCGGCGATGAAAAACAACGTCGCCCCGGCCAAGTGGACGTACGAAAACATCGACTACATGAAGACCCAG‑CTCAAGAGCCTGGGCCTGGCCATCGACTGGGCACGTGAAGTCACCACCt > 1:343466/1‑150 (MQ=255) tGGGACGCTTTCGGCATGCCCGCGGAAAACGCGGCGATGAAAAACAACGTCGCCCCGGCCAAGTGGACGTACGAAAACATCGACTACATGAAGACCCAG‑CTCAAGAGCCTGGGCCTGGCCATCGACTGGGCACGt < 2:586327/135‑1 (MQ=255) tGGGACGCTTTCGGCATGCCCGCGGAAAACGCGGCGATGAAAAACAACGTCGCCCCGGCCAAGTGGACGTACGAAAACATCGACTACATGAAGACCCAG‑CTCAAGAGCCTGGGCCTGGCCATCGACTGGGCACGt > 1:586327/1‑135 (MQ=255) ggACGCTTTCGGCATGCCCGCGGAAAACGCGGCGGTGAAAAACAACGTCGCCCCGGCCAAGTGGACGTACGAAAACATCGACTACATGAAGACCCAG‑CTCAAGAGCCTGGGCCTGGCCATCGACGGGGCACATGAAGTCACCACCTGcca > 2:335458/1‑148 (MQ=255) gACGCTTTCGGCATGCCCGCGGAAAACGCGGCGATGAAAAACAACGTCGCCCCGGCCAAGTGGACGTACGAAAACAGCGACTACAGGAAGACCCAG‑CTCAAGAGCCTGGGCCTGGCCATCGACTGGGCACGTGAAGTCACCACCTGCaaa < 1:334295/150‑1 (MQ=255) tttCGGCATGCCCGCGGAAAACGCGGCGATGAAAAACAACGTCGCCCCGGCCAAGTGGACGTACGAAAACATCGACTACATGAAGACCCAG‑CTCAAGAgcctgggc > 2:848539/1‑106 (MQ=255) tttCGGCATGCCCGCGGAAAACGCGGCGATGAAAAACAACGTCGCCCCGGCCAAGTGGACGTACGAAAACATCGACTACATGAAGACCCAG‑CTCAAGAgcctgggc < 1:848539/106‑1 (MQ=255) ccGCGGAAAACGCGGCGATGAAAAACAACGTCGCCCCGGCCAAGTGGACGTACGAAAACATCGACTACATGAAGACCCAG‑CTCAAGAGCCTGGGCCTGGCCATCGACTGGGCACGTGAAGTCACCACCTGCAAACCCGACTATTACCGtt < 1:146419/150‑1 (MQ=255) cgATGAAAAACAACGTCGCCCCGGCCAAGTGGACGTACGAAAACATCGACTACATGAAGACCCAG‑CTCAAGAGCCTGGGCCTGGCCATCGACTGGGCACGTGAAGTCACCACCTGCAAACCCGACTATTACCGTTGGGAGCAGTGGCTGt < 2:1003044/150‑1 (MQ=255) gATGAAAAACAACGCCGCCCCGGCCAAGTGGACGTACGAAAACATCGACTACATGAAGACCCAG‑CTCAAGAGCCTGGGCCTGGCCATCGACTCGGCACGTGAAGTCACCACCTGCAAACCCGACTATTACCGTTGGGAGCAGTGGCTGtt > 2:194207/1‑150 (MQ=255) gAAAAACAACGTCGCCCCGGCCAAGTGGACGTACGAAAACATCGACTACATGAAGACCCAG‑CTCAAGAGCCTGGGCCTGGCCATCGACTGGGCACGTGAAGTCACCACCTGCAAACCCGACTATTACCGTTGGGAGCAGTGGCTGTTCAc > 1:754429/1‑150 (MQ=255) gAAAAACAACGTCGCCCCGGCCAAGTGGACGTACGAAAACATCGACTACATGAAGACCCAG‑CTCAAGAGCCTGGGCCTGGCCATCGACTGGGCACGTGAAGTCACCACCTGCAAACCCGACTATTACCGTTGGGAGCAGTGGCTGCTCAc < 1:837741/150‑1 (MQ=255) aaaaCAACGTCGCCCCGGCCAAGTGGACGTACGAAAACATCGACTACATGAAGACCCAG‑CTCAAGAGCCTGGGCCTGGCCATCGACTGGGCACGTGAAGTCACCACCTGCAAACCCGACTATTACCGTTGGGAGCAGTGGCTGTTCAccc > 1:1029035/1‑150 (MQ=255) aacaacGTCGCCCCGGCCAAGTGGACGTACGAAAACATCGACTACATGAAGACCCAG‑CTCAAGAGCCTGGGCCTGGCCATCGACTGGGCACGTGAAGTCACCACCTGCAAACCCGACTATTACCGTTGGGAGCAGTGGCTGTTCACCCGc > 1:656435/1‑150 (MQ=255) ccccGGCCAAGTGGACGTACGAAAACATCGACTACATGAAGACCCAG‑CTCAAGAGCCTGGGCCTGGCCATCGACTGGGCACGTGAAGTCACCACCTGCAAACCCGACTATTACCGTTGGGAGCAGTGGCTGTTCACCCGCCTGTTCGaaa > 2:597560/1‑150 (MQ=255) cccGGCCAAGTGGACGTACGAAAACATCGACTACATGAAGACCCAG‑CTCAAGAGCCTGGGCCTGGCCATCGACTGGGCACGTGAAGTCACCACCTGCAAACCCGACTATTACCGTTGGGAGCAGTGGCTGTTCACCCGCCTGTTCGaaaa > 2:347769/1‑150 (MQ=255) cGGCCAAGTGGACGTACGAAAACATCGACTACATGAAGACCCAG‑CTCAAGAGCCTGGGCCTGGCTATCGACTGGGCACGTGAAGTCACCACCTGCAAACCCGACTATTACCGTTGGGAGCAGTGGCTGTTCACCCGCCTGTTCGAAAAAg < 1:245421/150‑1 (MQ=255) cGGCCAAGTGGACGTACGAAAACATCGACTACATGAAGACCCAG‑CTCAAGAGCCTGGGCCTGGCTATCGACTGGGCACGTGAAGTCACCACCTGCAAACCCGACTATTACCGTTGGGAGCAGTGGCTGTTCACCCGCCTGTTCGAAAAAg > 2:245421/1‑150 (MQ=255) aaGTGGACGTACGAAAACATCGACTACATGAAGACCCAG‑CTCAAGAGCCTGGGCCTGGCCATCGACTGGGCACGTGAAGTCACCACCTGCAAACCCGACTATTACCGTTGGGAGCAGTGGCTGTTCACCCGCCTGTTCGAAAAAGGcatc < 1:347769/150‑1 (MQ=255) gTACGAAAACATCGACTACATGAAGACCCAG‑CTCAAGAGCCTGGGCCTGGCCATCGACTGGGCACGTGAAGTCACCACCTGCAAACCCGACTATTAcc > 2:59453/1‑98 (MQ=255) gTACGAAAACATCGACTACATGAAGACCCAG‑CTCAAGAGCCTGGGCCTGGCCATCGACTGGGCACGTGAAGTCACCACCTGCAAACCCGACTATTAcc < 1:59453/98‑1 (MQ=255) cGAAAACATCGACTACATGAGGTGCCAG‑CTCAAGAGCCTGGGCCTGGCCATCGACTGGGCACGTGAAGTCACCACCTGCAAACCCGACTATTACCGTTGGGAGCAGTGGCTGTTCACCCGCCTGTTCGAAAAAGGCATCATCTACCGCaa < 1:335458/150‑1 (MQ=255) cGAAAACATCGACTACATGAAGACCCAG‑CTCAAGAGCCTGGGCCTGGCCATCGACTGGGCACGTGAAGTCACCACCTGCAAACCCGACTATTACCGTTGGGAGCAGTGGCTGTTCACCCGTCTGTTCGAAAAAGGCATCATCTACCGCaa < 1:103276/150‑1 (MQ=255) aaaaCATCGACTACATGAAGACCCAG‑CTCAAGAGCCTGGGCCTGGCCATCGACTGGGCACGTGAAGTCACCACCTGCAAACCCGACTATTACCGTTGGGAGCAGTGGCTGTTCACCCGCCTGTTCGAAAAAGGCATCATCTACCGCAAGa > 2:601725/1‑150 (MQ=255) ccagtcgatggccaggCCCAGGCTCTTGAGC‑TGGGCCTGGCCATCGACTGGGCACGTGAAGTCACCACCTGCAAACCCGACTATTACCGTTGGGAGCa < 2:953797‑M1/82‑1 (MQ=255) ccagtcgatggccaggCCCAGGCTCTTGAGC‑TGGGCCTGGCCATCGACTGGGCACGTGAAGTCACCACCTGCAAACCCGACTATTACCGTTGGGAGCa > 1:953797‑M1/17‑98 (MQ=255) aTCGACTACATGAAGACCCAG‑CTCAAGAGCCTGGGCCTGGCCATCGACTGGGCACGTGAAGTCACCACCTGCAAACCCGACTATTACCGTTGGGAGCAGTGGCTGTTCACCCGCCTGTTCGAAAAAGGCATCATCTACCGCAAGAACGGt > 1:975530/1‑150 (MQ=255) gACTACATGAAGACCCAG‑CTCAAGAGCCTGGGCCTGGCCATCGACTGGGCACGTGAAGTCACCACCTGCAAACCCGACTATTACCGTTGGGAGCAGTGGCTGTTCACCCGCCTGTTCGAAAAAGGCATCATCTACCGCAAGAACGGTAcc < 2:1029035/150‑1 (MQ=255) cTACATGAAGACCCAG‑CTCAAGAGCCTGGGCCTGGCCATCGACTGGGCACGTGAAGTCACCACCTGCAAACCCGACTATTACCGTTGGGAGCAGTGGCTGTTCACCCGCCTGTTCGAAAAAGGCATCATCTACCGCAAGAACGGTACCGt < 1:194207/150‑1 (MQ=255) gAAGACCCAG‑CTCAAGAGCCTGGGCCTGGCCATCGACTGGGCACGTGAAGTCACCACCTGCAAACCCGACTATTACCGTTGGGAGCAGTGGCTGTTCACCCGCCTGTTCGAAAAAGGCATCATCTACCGCAAGAACGGTACCGTGAACTg > 1:999152/1‑150 (MQ=255) gAAGACCCAG‑CTCAAGAGCCTGGGCCTGGCCATCGACTGGGCACGTGAAGTCACCACCTGCAAACCCGACTATTACCGTTGGGAGCAGTGGCTGTTCACCCGCCTGTTCGAAAAAGGCATCATCTACCGCAAGAACGGTACCGTGAACTg > 1:656579/1‑150 (MQ=255) ccaggCCCAGGCTCTTGAGC‑TGGGCCTGGCCATCGACTGGGCACGTGAAGTCACCACCTGCAAACCCGACTATTAcc < 2:1043954‑M1/72‑1 (MQ=255) ccaggCCCAGGCTCTTGAGC‑TGGGCCTGGCCATCGACTGGGCACGTGAAGTCACCACCTGCAAACCCGACTATTAcc > 1:1043954‑M1/6‑77 (MQ=255) aCCCAG‑CTCAAGAGCCTGGGCCTGGCCATCGACTGGGCACGTGAAGTCACCACCTGCAAACCCGACTATTACCGTTGGGAGCAGTGGCTGTTCACCCGCCTGTTCGAAAAAGGCATCATCTACCGCAAGAACGGTACCGTGAACTGGGAc > 1:1111790/1‑150 (MQ=255) CCAG‑CTCAAGAGCCTGGGCCTGGCCATCGACTGGGCACGTGAAGTCACCCCCTGCAAACCCGACTATTACCGTTGGGAGCAGTGGCTGt > 2:1032266‑M1/1‑89 (MQ=255) CCAG‑CTCAAGAGCCTGGGCCTGGCCATCGACTGGGCACGTGAAGTCACCACCTGCAAACCCGACTATTACCGTTGGGAGCAGTGGCTGt < 1:1032266‑M1/89‑1 (MQ=255) G‑CTCAAGAGCCTGGGCCTGGCCATCGACTGGGCACGTGAAGTCACCACCTGCAAACCCGACTATTACCGTTGGGAGCAGTGGCTGTTCACCCGCCTGTTCGAAAAAGGCATCATCTACCGCAAGAACGGTACCGTGAACTGGGACCCGGc > 2:270314‑M1/1‑150 (MQ=255) cTCAAGAGCCTGGGCCTGGCCATCGACTGGGCACGTGAAGTCACCACCTGCAAACCCGACTATTACCGTTGGGAGCAGTGGCTGTTCACCCGCCTGTTCGAAAAAGGCATCATCTACCGCAAGAACGGTACCGTGAACTGGGACCcggcg < 2:939154/150‑1 (MQ=255) aaGAGCCTGGGCCTGGCCATCGACTGGGCACGTGAAGTCACCACCTGCAAACCCGACTATTACCGTTGGGAGCAGTGGCTGTTCACCCGCCTGTTCGAAAAAGGCATCATCTACCGCAAGAACGGTACCGTGAACTGGGACCCGGCGGAc < 2:1111790/150‑1 (MQ=255) aaGAGCCTGGGCCTGGCCATCGACTGGGCACGTGAAGTCACCACCTGCAAACCCGACTATTACCGTTGGGAGCAGTGGCTGTTCACCCGCCTGTTCGAAAAAGGCATCATCTACCGCAAGAACGGTACCGTGAACTGGGACCCGGCGGAc < 1:601725/150‑1 (MQ=255) | CCGCTACCAGCGCATGCTGGGCAAGAACGTCCTGCAGCCGATGGGCTGGGACGCTTTCGGCATGCCCGCGGAAAACGCGGCGATGAAAAACAACGTCGCCCCGGCCAAGTGGACGTACGAAAACATCGACTACATGAAGACCCAG‑CTCAAGAGCCTGGGCCTGGCCATCGACTGGGCACGTGAAGTCACCACCTGCAAACCCGACTATTACCGTTGGGAGCAGTGGCTGTTCACCCGCCTGTTCGAAAAAGGCATCATCTACCGCAAGAACGGTACCGTGAACTGGGACCCGGCGGAC > NC_002947/5456505‑5456802 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |