Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 483,565 C→A 8.7% G293C (GGC→TGC)  yeaG ← protein kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947483,5650CA 8.7% 136.7 / 2.8 46G293C (GGC→TGC) yeaGprotein kinase
Reads supporting (aligned to +/- strand):  ref base C (21/21);  new base A (2/2);  total (23/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.16e-01

CAGCAGTTTGTCGTAGATCTTGATTTCATCGCTGACCCGCAGGCAGTACGGCACCTTGACGATATAGATCCTGTCGATGAACGCTTCGTTGTTCTTGTTGTTACGGAAGGTGTGCCACTCCGATTCGTTGGAGTGCGCCAGCAGAATGCCGGTATAGGGGATCGCCCCAAGGCCTTCGGTACTGTTGTAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCAGGACCTTGATCGGCGCCTTGAACATTTCGACGAATTCCATCAGGCCCTGGTTGGCCCGGCAAAGCGCGCCCGA  >  NC_002947/483416‑483712
                                                                                                                                                     |                                                                                                                                                   
cagcagTTTGTCGTAGATCTTGATTTCATCGCTGACCCGCAGGCAGTACGGCACCTTGACGATATAGATCCTGTCGATGAACGCTTCGTTGTTCTTGTTGTTACGGAAGGTGTGCCACTCCGATTCGTTGGAGTGCGCCAGCAGAATGcc                                                                                                                                                     >  1:937273/1‑150 (MQ=255)
cagcagTTTGTCGTAGATCTTGATTTCATCGCTGACCCGCAGGCAGTACGGCACCTTGACGATATAGATCCTGTCGATGAACGCTTCGTTGTTCTTGTTGTTACGGAAGGTGTGCCACTCCGATTCGTTGGAGTGCGCCAGCAGAATGcc                                                                                                                                                     >  1:717064/1‑150 (MQ=255)
 agcagTTTGTCGTAGATCTTGATTTCATCGCTGACCCGCAGGCAGTACGGCACCTTGACGATATAGATCCTGTCGATGAACGCTTCGTTGTTCTTGTTGTTACGGAAGGTGTGCCACTCCGATTCGTTGGAGTGCGCCAGCAGAATGCCg                                                                                                                                                    <  2:503544/150‑1 (MQ=255)
       ttGTCGTAGATCTTGATTTCATCGCTGACCCGCAGGCAGTACGGCACCTTGACGATATAGATCCTGTCGATGAACGCTTCGTTGTTCTTGTTGTTACGGAAGGTGTGCCACTCCGATTCGTTGGAGTGCGCCAGCAGAATGCCGGTATAg                                                                                                                                              <  1:818398/150‑1 (MQ=255)
        tGTCGTAGATCTTGATTTCATCGCTGACCCGCAGGCAGTACGGCACCTTGACGATATAGATCCTGTCGATGAACGCTTCGTTGTTCTTGTTGTTACGGAAGGTGTGCCACTCCGATTCGTTGGAGTGCGCCAGCAGAATGCCGGTATAgg                                                                                                                                             <  2:808857/150‑1 (MQ=255)
           cGTAGATCTTGATTTCATCGCTGACCCGCAGGCAGTACGGCACCTTGACGATATAGATCCTGTCGATGAACGCTTCGTTGTTCTTGTTGTTACGGAAGGTGTGCCACTCCGATTCGTTGGAGTGCGCCAGCAGAATGCCGGTATAGGGGa                                                                                                                                          <  2:191749/150‑1 (MQ=255)
                       tttCATCGCTGACCCGCAGGCAGTACGGCACCTTGACGATATAGATCCTGTCGATGAACGCTTCGTTGTTCTTGTTGTTACGGAAGGTGTGCCACTCCGATTCGTTGGAGTGCGCCAGCAGAATGCCGGTATAGGGGATCGCCCCAAGGc                                                                                                                              >  1:776489/1‑150 (MQ=255)
                            tCGCTGACCCGCAGGCAGTACGGCACCTTGACGATATAGATCCTGTCGATGAACGCTTCGTTGTTCTTGTTGTTACGGAAGGTGTGCCACTCCGATTCGTTGGAGTGCGCCAGCAGAATGCCGGTATAGGGGATCGCCCCAAGGCCTTCg                                                                                                                         >  1:717026/1‑150 (MQ=255)
                                   cccGCAGGCAGTACGGCACCTTGACGATATAGATCCTGTCGATGAACGCTTCGTTGTTCTTGTTGTTACGGAAGGTGTGCAACTCCGATTCTTTGGAGTTCTCCTGCAGAATGCCGGTATAGGGGATCGCCCCAAGGCCTTCGGTACtgt                                                                                                                  >  2:970134/1‑150 (MQ=255)
                                    ccGCAGGCAGTACGGCACCTTGACGATATAGATCCTGTCGATGAACGCTTCGTTGTTCTTGTTGTTACGGAAGGTGTGCCACTCCGATTCGTTGGAGTGCGCCAGCAGAATGCCGGTATAGGGGATCGCCCCAAGGCCTTCGGTACtgtt                                                                                                                 <  2:780946/150‑1 (MQ=255)
                                                 ggCACCTTGACGATATAGATCCTGTCGATGAACGCTTCGTTGTTCTTGTTGTTACGGAAGGTGTGCCACTCCGATTCGTTGGAGTGCGCCAGCAGAATGCAGGTATAgg                                                                                                                                             <  2:1080546/109‑1 (MQ=255)
                                                 ggCACCTTGACGATATAGATCCTGTCGATGAACGCTTCGTTGTTCTTGTTGTTACGGAAGGTGTGCCACTCCGATTCGTTGGAGTGCGCCAGCAGAATGCAGGTATAgg                                                                                                                                             <  2:1080637/109‑1 (MQ=255)
                                                 ggCACCTTGACGATATAGATCCTGTCGATGAACGCTTCGTTGTTCTTGTTGTTACGGAAGGTGTGCCACTCCGATTCGTTGGAGTGCGCCAGCAGAATGCAGGTATAgg                                                                                                                                             >  1:1080637/1‑109 (MQ=255)
                                                 ggCACCTTGACGATATAGATCCTGTCGATGAACGCTTCGTTGTTCTTGTTGTTACGGAAGGTGTGCCACTCCGATTCGTTGGAGTGCGCCAGCAGAATGCAGGTATAgg                                                                                                                                             >  1:1080546/1‑109 (MQ=255)
                                                       ttGACGATATAGATCCTGTCGATGAACGCTTCGTTGTTCTTGTTGTTACGGAAGGTGTGCCACTCCGATTCGTTGGAGTGCGCCAGCAGAATGCCGGTATAGGGGATCGCCCCAAGGCCTTCGGTACTGTTGTAGTTGCCTTc                                                                                                     >  2:149592/1‑143 (MQ=255)
                                                       ttGACGATATAGATCCTGTCGATGAACGCTTCGTTGTTCTTGTTGTTACGGAAGGTGTGCCACTCCGATTCGTTGGAGTGCGCCAGCAGAATGCCGGTATAGGGGATCGCCCCAAGGCCTTCGGTACTGTTGTAGTTGCCTTc                                                                                                     <  1:149592/143‑1 (MQ=255)
                                                       ttGACGATATAGATCCTGTCGATGAACGCTTCGTTGTTCTTGTTGTTACGGAAGGTGTGCCACTCCGATTCGTTGGAGTGCGCCAGCAGAATGCCGGTATAGGGGATCGCCCCAAGGCCTTCGGTACTGTTGTAGTTGCCTTCCTGGGTg                                                                                              >  1:871833/1‑150 (MQ=255)
                                                       ttGACGATATAGATCCTGTCGATGAACGCTTCGTTGTTCTTGTTGTTACGGAAGGTGTGCCACTCCGATTCGTTGGAGTGCGCCAGCAGAATGCCGGTATAGGGGATCGCCCCAAGGCCTTCGGTACTGTTGTAGTTGCCTTCCTGGGTg                                                                                              <  2:489280/150‑1 (MQ=255)
                                                        tGACGATATAGATCCTGTCGATGAACGCTTCGTTGTTCTTGTTGTTACGGAAGGTGTGCCACTCCGATTCGTTGGAGTGCGCCAGCAGAATGCCGGTATAGGGGATCGCCCCAAGGCCTTCGGTACTGTTGTAGTTGCCTTCCTGGGTgg                                                                                             >  1:4546/1‑150 (MQ=255)
                                                                      cTGTCGATGAACGCTTCGTTGTTCTTGTTGTTACGGAAGGTGTGCCACTCCGATTCGTTGGAGTGCGCCAGCAGAATGCCGGTATAGGGGATCGCCCCAAGGCCTTCGGTACTGTTGTAGTTGCCTTCCTGGGTGGCGGTGAGCAAgggg                                                                               <  2:937273/150‑1 (MQ=255)
                                                                           gATGAACGCTTCGTTGTTCTTGTTGTTACGGAAGGTGTGCCACTCCGATTCGTTGGAGTGCGCCAGCAGAATGCCGGTATAGGGGATCGCCCCAAGGCCTTCGGTACTGTTGTAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCAg                                                                          <  2:221035/150‑1 (MQ=255)
                                                                            aTGAACGCTTCGTTGTTCTTGTTGTTACGGAAGGTGTGCCACTCCGATTCGTTGGAGTGCGCCAGCAGAATGCCGGTATAGGGGATCGCCCCAAGGCCTTCGGTACTGTTGTAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCa                                                                           >  1:1044523/1‑148 (MQ=255)
                                                                            aTGAACGCTTCGTTGTTCTTGTTGTTACGGAAGGTGTGCCACTCCGATTCGTTGGAGTGCGCCAGCAGAATGCCGGTATAGGGGATCGCCCCAAGGCCTTCGGTACTGTTGTAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCa                                                                           <  2:1044523/148‑1 (MQ=255)
                                                                             tGAACGCTTCGTTGTTCTTGTTGTTACGGAAGGTGTGCCACTCCGATTCGTTGGAGTGCGCCAGCAGAATGCCGGTATAGGGGATCGCCCCAAGGCCTTCGGTACTGTTGTAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCAGGa                                                                        <  2:4546/150‑1 (MQ=255)
                                                                                     tCGTTGTTCTTGTTGTTACGGAAGGTGTGCCACTCCGATTCGTTGGAGTGCGCCAGCAGAATGCCGGTATAGGGGATCGCCCCAAGGCCTTCGGTACTGTTGTAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCAGGACCTTGATc                                                                <  2:871833/150‑1 (MQ=255)
                                                                                       gttgttCTTGTTGTTACGGAAGGTGTGCCACTCCGATTCGTTGGAGTGCGCCAGCAGAATGCCGGTATAGGGGATCGCCCCAAGGCCTTCGGTACTGTTGTAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCAGGACCTTGATCgg                                                              >  2:502001/1‑150 (MQ=255)
                                                                                       gttgttCTTGTTGTTACGGAAGGTGTGCCACTCCGATTCGTTGGAGTGCGCCAGCAGAATGCCGGTATAGGGGATCGCCCCAAGGCCTTCGGTACTGTTGTAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCAGGACCTTGATCgg                                                              >  1:969981/1‑150 (MQ=255)
                                                                                        ttgttCTTGTTGTTACGGAAGGTGTGCCACTCCGATTCGTTGGAGTGCGCCAGCAGAATGCCGGTATAGGGGATCGCCCCAAGGCCTTCGGTACTGTTGTAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCAGGACCTTGATCGgc                                                             <  2:969981/150‑1 (MQ=255)
                                                                                          gttCTTGTTGTTACGGAAGGTGTGCCACTCCGATTCGTTGGAGTGCGCCAGCAGAATGCCGGTATAGGGGATCGCCCCAAGGCCTTCGGTACtgttg                                                                                                                >  1:1052906/1‑97 (MQ=255)
                                                                                          gttCTTGTTGTTACGGAAGGTGTGCCACTCCGATTCGTTGGAGTGCGCCAGCAGAATGCCGGTATAGGGGATCGCCCCAAGGCCTTCGGTACtgttg                                                                                                                <  2:1052906/97‑1 (MQ=255)
                                                                                                gttgttACGGAAGGTGTGCCACTCCGATTCGTTGGAGTGCGCCAGCAGAATGCCGGTATAGGGGATCGCCCCAAGGCCTTCGGTACTGTTGTAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCAGGACCTTGATCGGCGCCTTGaa                                                     <  1:983314/150‑1 (MQ=255)
                                                                                                 ttgttACGGAAGGTGTGCCACTCCGATTCGTTGGAGTGCGCCAGCAGAATGCCGGTATAgggg                                                                                                                                           >  1:237006/1‑63 (MQ=255)
                                                                                                 ttgttACGGAAGGTGTGCCACTCCGATTCGTTGGAGTGCGCCAGCAGAATGCCGGTATAgggg                                                                                                                                           <  2:237006/63‑1 (MQ=255)
                                                                                                    ttACGGAAGGTGTGCCACTCCGATTCGTTGGAGTGCGCCAGCAGAATGCCGGTATAGGGGATCGcc                                                                                                                                     <  2:44939/66‑1 (MQ=255)
                                                                                                    ttACGGAAGGTGTGCCACTCCGATTCGTTGGAGTGCGCCAGCAGAATGCCGGTATAGGGGATCGcc                                                                                                                                     >  1:44939/1‑66 (MQ=255)
                                                                                                     tACGGAAGGTGTGCCACTCCGATTCGTTGGAGTGCGCCAGCAGAATGCCGGTATAGGGGATCGCCCCAAGGCCTTCGGTACTGTTGTAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCAGGACCTTGATCGGCGCCTTGAACAttt                                                <  2:1117540/150‑1 (MQ=255)
                                                                                                              tgtgCCACTCCGATTCGTTGGAGTGCGCCAGCAGAATGCCGGTATAGGGGATCGCCCCAAGGCCTTCGGTACTGTTGTAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCAGGACCTTGATCGGCGCCTTGAACATTTCGACGAAtt                                       >  1:663318/1‑150 (MQ=255)
                                                                                                                tgCCACTCCGATTCGTTGGAGTGCGCCAGCAGAATGCCGGTATAGGGGATCGCCCCAAGGCCTTCGGTACTGTTGTAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCAGGACCTTGATCGGCGCCTTGAACATTTCGACGAATTcc                                     >  2:446608/1‑150 (MQ=255)
                                                                                                                tgCCACTCCGATTCGTTGGAGTGCGCCAGCAGAATGCCGGTATAGGGGATCGCCCCAAGGCCTTCGGTACTGTTGTAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCAGGACCTTGATCGGCGCCTTGAACATTTCGACGAATTcc                                     >  1:959035/1‑150 (MQ=255)
                                                                                                                  ccACTCCGATTCGTTGGAGTGCGCCAGCAGAATGCCGGTATAGGGGATCGCCCCAAGGCCTTCGGTACTGTTGTAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCAGGACCTTGATCGGCGCCTTGAACATTTCGACGAATTCCAt                                   >  2:113468/1‑150 (MQ=255)
                                                                                                                              gTTGGAGTGCGCCAGCAGAATGCCGGTATAGGGGATCGCCCCAAGGCCTTCGGTACTGTTGTAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCAGGACCTTGATCGGCGCCTTGAACATTTCGACGAATTCCATCAGGCCCTGGtt                       >  1:967696/1‑150 (MQ=255)
                                                                                                                               ttGGAGTGCGCCAGCAGAATGCCGGTATAGGGGATCGCCCCAAGGCCTTCGGTACTGTTGTAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCAGGACCTTGATCGGCGCCTTGAACATTTCGACGAATTCCATCAGGCCCTGGTTg                      >  2:501372/1‑150 (MQ=255)
                                                                                                                                tGGAGTGCGCCAGCAGAATGCCGGTATAGGGGATCGCCCCAAGGCCTTCGGTACTGTTGTAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCAGGACCTTGATCGGCGCCTTGAACATTTCGACGAATTCCATCAGGCCCTGGTTgg                     >  2:586544/1‑150 (MQ=255)
                                                                                                                                    gTGCGCCAGCAGAATGCCGGTATAGGGGATCGCCCCAAGGCCTTCGGTACTGTTGTAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCAGGACCTTGATCGGCGCCt                                                         <  2:873009/110‑1 (MQ=255)
                                                                                                                                    gTGCGCCAGCAGAATGCCGGTATAGGGGATCGCCCCAAGGCCTTCGGTACTGTTGTAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCAGGACCTTGATCGGCGCCt                                                         >  1:873009/1‑110 (MQ=255)
                                                                                                                                           agcagAATGCCGGTATAGGGGATCGCCCCAAGGCCTTCGGTACTGTTGTAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCAGGACCTTGATCGGCGCCTTGAACATTTCGACGAATTCCATCAGGCCCTGGTTGGCCCGGCAAAgc          >  2:321243/1‑150 (MQ=255)
                                                                                                                                             cagAATGCCGGTATAGGGGATCGCCCCAAGGCCTTCGGTACTGTTGTAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCAGGACCTTGATCGGCGCCTTGAACATTTCGACGAATTCCATCAGGCCCTGGTTGGCCCGGCAAAgcgc        <  2:663318/150‑1 (MQ=255)
                                                                                                                                                   gCCGGTATAGGGGATCGCCCCAAGGCCTTCGGTACTGTTGTAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCAGGACCTTGATCGGCGCCTTGAACATTTCGACGAATTCCATCAGGCCCTGGTTGGCCCGGCAAAGCGCGCCCGa  <  2:959035/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CAGCAGTTTGTCGTAGATCTTGATTTCATCGCTGACCCGCAGGCAGTACGGCACCTTGACGATATAGATCCTGTCGATGAACGCTTCGTTGTTCTTGTTGTTACGGAAGGTGTGCCACTCCGATTCGTTGGAGTGCGCCAGCAGAATGCCGGTATAGGGGATCGCCCCAAGGCCTTCGGTACTGTTGTAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCAGGACCTTGATCGGCGCCTTGAACATTTCGACGAATTCCATCAGGCCCTGGTTGGCCCGGCAAAGCGCGCCCGA  >  NC_002947/483416‑483712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑