Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,575,949 A→G 7.9% I84T (ATC→ACC)  motA ← flagellar motor rotation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,575,9490AG 7.9% 168.2 / 2.7 59I84T (ATC→ACC) motAflagellar motor rotation protein
Reads supporting (aligned to +/- strand):  ref base A (24/30);  new base G (3/2);  total (27/32)
Fisher's exact test for biased strand distribution p-value = 6.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATGATGCGCAGGTAATCGCAGACGAACGCGGTCATGCGCTCATCGGCCAGTACCGTCGGGTACTTGGCGAAGATCGGGCTGGCAGCGGCGTCCTCGATGTCGGCCTCGATGGCCATCATGCCTTCGCGACGGCTCTTGTTGAGGATTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATGGCTGAAGCGTGAGCCGAACATCTTCATCGACTTCTTGATGACGTGCATGGTCATGTGGCCAGGGTTGGCCTGCAGGAATGCACCAAAGGCCGCACCGCCGATGATCAGT  >  NC_002947/5575804‑5576098
                                                                                                                                                 |                                                                                                                                                     
cATGATGCGCAGGTAATCGCAGACGAACGCGGTCATGCGCTCATCGGCCAGTACCGTCGGGTACTTGGCGAAGATCGGGCTGGCAGCGGCGTCCTCGATGTCGGCCTCGATGGCCATCATGCCTTCGCGACGGCTCTTGTTGAGGATTTc                                                                                                                                                   >  1:765234/1‑150 (MQ=255)
 atgatgCGCAGGTAATCGCAGACGAACGCGGTCATGCGCTCATCGGCCAGTACCGTCGGGTACTTGGCGAAGATCGGGCTGGCAGCGGCGTCCTCGATGTCGGCCTCGATGGCCATCATGCCTTCGCGACGGCTCTTGTTGAGGATTTCg                                                                                                                                                  >  1:714268/1‑150 (MQ=255)
      gcgcAGGTAATCGCAGACGAACGCGGTCATGCGCTCATCGGCCAGTACCGTCGGGTACTTGGCGAAGATCGGGCTGGCAGCGGCGTCCTCGATGTCGGCCTCGATGGCCATCATGCCTTCGCGACGGCTCTTGTTGAGGATTTCGTaact                                                                                                                                             <  1:715250/150‑3 (MQ=255)
          aGGNAATCGCAGACGAACGCGGTCATGCGCTCATCGGCCAGTACCGTCGGGTACTTGGCGAAGATCGGGCTGGCAGCGGCGTCCTCGATGTCGGCCTCGATGGCCATCATGCCTTCGCGACGGCTCTTGTTGAGGATTTCGTAAACGAgg                                                                                                                                         >  2:693896/1‑150 (MQ=255)
                  gCAGACGAACGCGGTCATGCGCTCATCGGCCCGTACCGTCCGGTACGTGGCGAAGGTCGGGCTGGCAGCGGCGTCCTCGATGTCGGCCTCGATGGCCATCATGCCTTCGCGACGGCTCTTGTTGAGGATTTCGTAAACGAGGCCCAGCAc                                                                                                                                 <  2:675507/150‑1 (MQ=255)
                  gCAGACGAACGCGGTCATGCGCTCATCGGCCAGTACCGTCGGGTACTTGGCGAAGATCGGGCTGGCAGCGGCGTCCTCGATGTCGGCCTCGATGGCCATCATGCCTTCGCGACGGCTCTTGTTGAGGATTTCGTAAACGAGGCCCAGCAc                                                                                                                                 <  1:1116412/150‑1 (MQ=255)
                       cGAACGCGGTCATGCGCTCATCGGCCAGTACCGTCGGGTACTTGGCGAAGATCGGGCTGGCAGCGGCGTCCTCGATGTCGGCCTCGATGGCCATCATGCCTTCGCGACGGCTCTTGTTGAGGATTTCGTAAACGAGGCCCAGCACCTCCa                                                                                                                            <  2:270344/150‑1 (MQ=255)
                        gAACGCGGTCATGCGCTCATCGGCCAGTACCGTCGGGTACTTGGCGAAGATCGGGCTGGCAGCGGCGTCCTCGATGTCGGCCTCGATGGCCATCATGCCTTCGCGACGGCTCTTGTTGAGGATTTCGTAAACGAGGCCCAGCACCTACAg                                                                                                                           <  1:588184/150‑1 (MQ=255)
                                     cgcTCATCGGCCAGTACCGTCGGGTACTTGGCGAAGATCGGGCTGGCAGCGCCGTCTTCGATGTCGGCCTCGATGGCCATCATGCCTTCGCGACGGCTCTTGTTGAGGATTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATgg                                                                                                              <  2:309530/150‑1 (MQ=255)
                                           tCGGCCAGTACCGTCGGGTACTTGGCGAAGATCGGGCTGCCAGCGGCGTCCTCGATGTCGGCCTCGATGGCCATCATGCCTTCGCGACGGCTCTTGTTGAGGATTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATGGCTGCAg                                                                                                        >  2:518565/1‑150 (MQ=255)
                                               ccAGTACCGTCGGGTACTTGGCGAAGATCGGGCTGGCAGCGGCGTCCTCGATGTCGGCCTCGATGGCAATCTAGCCTTCGCGCCGGGTCTTTTTGAGGATTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATAGCTGGAGCGTg                                                                                                    >  1:78612/1‑150 (MQ=255)
                                                      cGTCGGGTACTTGGCGAAGATCGGGCTGGCAGCGGCGTCCTCGATGTCGGCCTCGATGGCCATCATGCCTTCGCGACGGCTCTTGTTGAGGATTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATGGCTGaa                                                                                                         <  1:141960/138‑1 (MQ=255)
                                                      cGTCGGGTACTTGGCGAAGATCGGGCTGGCAGCGGCGTCCTCGATGTCGGCCTCGATGGCCATCATGCCTTCGCGACGGCTCTTGTTGAGGATTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATGGCTGaa                                                                                                         >  2:141960/1‑138 (MQ=255)
                                                       gTCGGGTACTTGGCTAAGATCGGGCTGGCAGCGGCGTCCTCGATGTCGGCCTCGATGGCCATCATGCCTTCGCGACGGCTCTTGTTGAGGATTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATGGCTGAAGCGTGAGCCGAAc                                                                                            <  2:733862/150‑1 (MQ=255)
                                                            gTACTTGGCGAAGATCGGGCTGGCAGCGGCGTCCTCGATGTCGGCCTCGATGGCCATCATGCCTTCGCGACGGCTCTTGTTGAGGATTTCGTAAACGAGGcc                                                                                                                                       <  1:238480/102‑1 (MQ=255)
                                                            gTACTTGGCGAAGATCGGGCTGGCAGCGGCGTCCTCGATGTCGGCCTCGATGGCCATCATGCCTTCGCGACGGCTCTTGTTGAGGATTTCGTAAACGAGGcc                                                                                                                                       >  2:238480/1‑102 (MQ=255)
                                                              aCTTGGCGAAGATCGGGCTGGCAGCGGCGTCCTCGATGTCGGCCTCGATGGCCATCATGCCTTCGCGACGGCTCTTGTTGAGGATTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATGGCTGACGCGTGCGGCGCACATCTTCa                                                                                     >  2:6128/1‑150 (MQ=255)
                                                               cTTGGCGAAGATCGGGCTGGCAGCGGCGTCCTCGATGTCGGCCTCGATGGCCATCATGCCTTCGCGACGGCTCTTGTTGAGGATTTCGTAAACGAGGCCCAGCACCTCAAGGTAGAAGGCATGGCTGAAGCGTGAGCCGAACATCTTCAt                                                                                    <  1:693896/150‑1 (MQ=255)
                                                                  ggCGAAGATCGGGCTGGCAGCGGCGTCCTCGATGTCGGCCTCGATGGCCATCATGCCTTCGCGACGGCTCTTGTTGAGGATTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATGGCTGAAGCGTGAGCCGAACATCTTCATCGa                                                                                 >  2:1045694/1‑150 (MQ=255)
                                                                   gCGAAGATCGGGCTGGCAGCGGCGTCCTCGATGTCGGCCTCGATGGCCATCATGCCTTCGCGACGGCTCTTGTTGAGGATTTCGTAAACGa                                                                                                                                           <  2:956875/91‑1 (MQ=255)
                                                                   gCGAAGATCGGGCTGGCAGCGGCGTCCTCGATGTCGGCCTCGATGGCCATCATGCCTTCGCGACGGCTCTTGTTGAGGATTTCGTAAACGa                                                                                                                                           >  1:956875/1‑91 (MQ=255)
                                                                     gAAGATCGGGCTGGCAGCGGCGTCCTCGATGTCGGCCTCGATGGCCATCATGCCTTCGCGACGGCTCTTGTTGAGGATTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATGGCTGAAGCGTGAGCCGAACATCTTCATCGActt                                                                              >  2:1072459/1‑150 (MQ=255)
                                                                              gCTGGCAGCGGCGTCCTCGATGTCGGCCTCCACGGCCATCATGCCTTCGCGACGGCTCTTGTTGAGGATTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATGGCTGAAGCGTGAGCCGAACATCTTCATCGACTTCTTGATGAc                                                                     <  1:852332/150‑1 (MQ=255)
                                                                                          gTCCTCGATGTCGGCCTCGATGGCCATCATGCCTTCGCGACGGCTCTTGTTGAGGATTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATGGCTGAAGCGTGAGCCGAACATCTTCATCGACTTCTTGATGACGTGCATGGTCAt                                                         <  2:833389/150‑1 (MQ=255)
                                                                                             cTCGATGTCGGCCTCGATGGCCATCATGCCTTCGCGACGGCTCTTGTTGAGGGGTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATGGCTGAAGCGTGAGCCGAACATCTTCATCGACTTCTTGATGACGTGCATGGTCAtgtg                                                      >  1:576666/1‑150 (MQ=255)
                                                                                             cTCGATGTCGGCCTCGATGGCCATCATGCCTTCGCGACGGCTCTTGTTGAGGATTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATGGCTGAAGCGTGAGCCGAACATCTTCATCGACTTCTTGATGACGTGCATGGTCAtgtg                                                      <  1:1072459/150‑1 (MQ=255)
                                                                                                  tGTCGGCCTCGATGGCCATCATGCCTTCGCGACGGCTCTTGTTGAGGATTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATGGCTGAAGCGTGAGCCGAACATCTTCATCGACTTCTTGATGACGTGCATGGTCACGTGGCCAg                                                 <  1:1045694/150‑1 (MQ=255)
                                                                                                   gTCGGCCTCGATGGCCATCATGCCTTCGCGACGGCTCTTGTTGAGGATTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATgg                                                                                                              >  1:43927/1‑88 (MQ=255)
                                                                                                   gTCGGCCTCGATGGCCATCATGCCTTCGCGACGGCTCTTGTTGAGGATTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATgg                                                                                                              <  2:43927/88‑1 (MQ=255)
                                                                                                      ggCCTCGATGGCCATCATGCCTTCGCGACGGCTCTTGTTGAGGATTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATGGCTGAAGCGTGAGCCGAACATCTTCATCGActtct                                                                            >  2:783284/1‑119 (MQ=255)
                                                                                                      ggCCTCGATGGCCATCATGCCTTCGCGACGGCTCTTGTTGAGGATTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATGGCTGAAGCGTGAGCCGAACATCTTCATCGActtct                                                                            <  1:783284/119‑1 (MQ=255)
                                                                                                      ggCCTCGATGGCCATCATGCCTTCGCGACGGCTCTTGTTGAGGATTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATGGCTGAAGCGTGAGCCGAACATCTTCATCGACTTCTTGATGACGTGCATGGTCATGTGGCCAGGGtt                                             <  2:714268/150‑1 (MQ=255)
                                                                                                       gCCTCGATGGCCATCATGCCTTCGCGACGGCTCTTGTTGAGGGTTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATGGCTGAAGCGTGAGcc                                                                                                <  2:809061/98‑1 (MQ=255)
                                                                                                       gCCTCGATGGCCATCATGCCTTCGCGACGGCTCTTGTTGAGGGTTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATGGCTGAAGCGTGAGcc                                                                                                <  2:808989/98‑1 (MQ=255)
                                                                                                       gCCTCGATGGCCATCATGCCTTCGCGACGGCTCTTGTTGAGGGTTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATGGCTGAAGCGTGAGcc                                                                                                >  1:809061/1‑98 (MQ=255)
                                                                                                       gCCTCGATGGCCATCATGCCTTCGCGACGGCTCTTGTTGAGGGTTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATGGCTGAAGCGTGAGcc                                                                                                >  1:808989/1‑98 (MQ=255)
                                                                                                        ccTCGATGGCCATCATGCCTTCGCGACGGCTCTTGTTGAGGATTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATGGCTGAAGCGTGAGCCGAACATCTTCATCGACTTCTTGATGACGTGCATGGTCAtgtg                                                      >  2:1023020/1‑139 (MQ=255)
                                                                                                        ccTCGATGGCCATCATGCCTTCGCGACGGCTCTTGTTGAGGATTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATGGCTGAAGCGTGAGCCGAACATCTTCATCGACTTCTTGATGACGTGCATGGTCAtgtg                                                      <  1:1023020/139‑1 (MQ=255)
                                                                                                        ccTCGATGGCCATCATGCCTTCGCGACGGCTCTTGTTGAGGATTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATGGCTGAAGCGTGAGCCGAACATCTTCATCGACTTCTTGATGACGTGCATGGTCATGTGGCCAGGGTTgg                                           >  2:8916/1‑150 (MQ=255)
                                                                                                         cTCGATGGCCATCATGCCTTCGCGACGGCTCTTGTTGAGGATTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATGGCTGAAGCGTGAGCCGAACATCTTCATCGACTTCTTGATGACGTGCATGGTCATGTGGCCAGGGTTGGc                                          <  2:110953/150‑1 (MQ=255)
                                                                                                         cTCGATGGCCATCATGCCTTCGCGACGGCTCTTGTTGAGGATTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATGGCTGAAGCGTGAGCCGAACATCTTCATCGACTTCTTGATGACGTGCATGGTCATGTGGCCAGGGTTGGc                                          >  1:110953/1‑150 (MQ=255)
                                                                                                            gATGGCCATCATGCCTTCGCGACGGCTCTTGTTGAGGATTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATGGCTGAAGCGTGAGCCGAACATCTTCATCGACTTCTTGATGACGTGCATGGTCATGTGGCCAGGGTTg                                            >  2:993666/1‑145 (MQ=255)
                                                                                                            gATGGCCATCATGCCTTCGCGACGGCTCTTGTTGAGGATTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATGGCTGAAGCGTGAGCCGAACATCTTCATCGACTTCTTGATGACGTGCATGGTCATGTGGCCAGGGTTg                                            <  1:993666/145‑1 (MQ=255)
                                                                                                              tGGCCATCATGCCTTCGCGACGGCTCTTGTTGAGGATTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATGGCTGAAGCGTGAGCCGAACATCTTCATCGACTTCTTGATGACGTGCATGGTCATGTGGCCAGGGTTGGCCTGCa                                     <  1:6128/150‑1 (MQ=255)
                                                                                                                 ccATCATGCCTTCGCGACGGCTCTTGTTGAGGATTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATGGCTGAAGCGTGAGCCGAACATCTTCAACTGGTGCTTGATGACGTGCATGGTCATGTGGCCAGGGTTGGCCTGCAGGa                                  >  1:67899/1‑150 (MQ=255)
                                                                                                                  catcatGCCTTCGCGACGGCTCTTGTTGAGGATTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATGGCTGAAGCGTGGGCCGAAAATCTTCATAGACATCTTGGCGACGAGCATTGTCATATGGCCAGTGTTGGCCTGCAGGaa                                 <  2:11929/150‑1 (MQ=255)
                                                                                                                       tGCCTTCGCGACGGCTCTTGTTGAGGATTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATGGCTGAAGCGTGAGCCGAACATCTTCATCGACTTCTTGATGACGTGCATGGTCATGTGGCCAGGGTTGGCCTGCAGGAATGCAc                            <  2:78612/150‑1 (MQ=255)
                                                                                                                           ttCGCGACGGCTCTTGTTGAGGATTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATGGCTGAAGCGTGAGCCGAACATCTTCATCGACTTCTTGATGACGTGCATGGTCATGTGGCCAGGGTTGGCCTGCAGGAATGCACCaaa                        <  2:765234/150‑1 (MQ=255)
                                                                                                                                 aCGGCTCTTGTTGAGGATTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATGGCTGAAGCGTGAGCCGAACATCTTCATCGACTTCTTGATGACGTGCATGGTCATGTGGCCAGGGTTGGCCTGCa                                     <  2:98080/131‑1 (MQ=255)
                                                                                                                                 aCGGCTCTTGTTGAGGATTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATGGCTGAAGCGTGAGCCGAACATCTTCATCGACTTCTTGATGACGTGCATGGTCATGTGGCCAGGGTTGGCCTGCa                                     >  1:98080/1‑131 (MQ=255)
                                                                                                                                    gCTCTTGTTGAGGATTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATGGCTGAAGCGTGAGCCGAACATCTTCATCGACTTCTtg                                                                          <  2:1130987/91‑1 (MQ=255)
                                                                                                                                    gCTCTTGTTGAGGATTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATGGCTGAAGCGTGAGCCGAACATCTTCATCGACTTCTtg                                                                          >  1:1130987/1‑91 (MQ=255)
                                                                                                                                     ctctTGTTGAGGATTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATGGCTGAAGCGTGAGCCGAACATCTTCATCGACTTCTTGATGACGTGCATGGTCATGTGGCCAGGGTTGGCCTGCAGGAAt                                <  2:1102994/132‑1 (MQ=255)
                                                                                                                                     ctctTGTTGAGGATTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATGGCTGAAGCGTGAGCCGAACATCTTCATCGACTTCTTGATGACGTGCATGGTCATGTGGCCAGGGTTGGCCTGCAGGAAt                                >  1:1102994/1‑132 (MQ=255)
                                                                                                                                      tctTGTTGAGGATTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATGGCTGAAGCGTGAGCCGAACATCTTCATCGACTTCTTGATGACGTGCATGGTCATGTGGCCAGGGTTGGCCTGCAGGAATGCACCAAAGGCCGCAccgc             >  1:97601/1‑150 (MQ=255)
                                                                                                                                           ttgAGGATTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATAGCTGAAGCGTGAGCCGAACATCTTCATCGACTTCTTGATGACGTGCATGGTCATGTGGCCAGGGTTg                                            >  1:73291/1‑114 (MQ=255)
                                                                                                                                           ttgAGGATTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATAGCTGAAGCGTGAGCCGAACATCTTCATCGACTTCTTGATGACGTGCATGGTCATGTGGCCAGGGTTg                                            <  2:73291/114‑1 (MQ=255)
                                                                                                                                             gAGGATTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATGGCTGAAGCGTGAGCCGAACATCTTCATCGACTTCTTGATGACGTGCATGGTCATGTGGCCAGGGTTGGCCTGCAGGAATGCACCAAAGGCCGCACCGCCgatgat      <  2:97601/150‑1 (MQ=255)
                                                                                                                                             gAGGATTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATGGCTGAAGCGTGAGCCGAACATCTTCATCGACTTCTTGATGACGTGCATGGTCATGTGGCCAGGGTTGGCCTGCAGGAATGCACCAAAGGCCGCACCGCCgatgat      >  2:335878/1‑150 (MQ=255)
                                                                                                                                                 aTTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATGGCTGAAGCGTGAGCCGAACATCTTCATCGACTTCTTGATGACGTGCATGGTCATGTGGCCAGGGTTGGCCTGCAGGAATGCACCAAAGGCCGCACCGCCGATGATCAGt  >  2:787094/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
CATGATGCGCAGGTAATCGCAGACGAACGCGGTCATGCGCTCATCGGCCAGTACCGTCGGGTACTTGGCGAAGATCGGGCTGGCAGCGGCGTCCTCGATGTCGGCCTCGATGGCCATCATGCCTTCGCGACGGCTCTTGTTGAGGATTTCGTAAACGAGGCCCAGCACCTCCAGGTAGAAGGCATGGCTGAAGCGTGAGCCGAACATCTTCATCGACTTCTTGATGACGTGCATGGTCATGTGGCCAGGGTTGGCCTGCAGGAATGCACCAAAGGCCGCACCGCCGATGATCAGT  >  NC_002947/5575804‑5576098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑