Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 484,695 G→C 9.4% intergenic (‑254/+29) yeaG ← / ← glpE protein kinase/thiosulfate sulfurtransferase GlpE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947484,6950GC 9.4% 126.5 / 4.6 43intergenic (‑254/+29)yeaG/glpEprotein kinase/thiosulfate sulfurtransferase GlpE
Reads supporting (aligned to +/- strand):  ref base G (18/20);  new base C (2/2);  total (20/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

GACCCGCACGCCGGTGTACCGGCTCTCCCCTTTTTGGATGGCCTGAGCTTAAGCGTAGTTGGTTATCTGCAAGGTAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAGGTCGCGCGCCACAGTTCGAAGCCTCCGTCCACGCTGTACACGTCGGAGAAACCCTGGCCTACCAGGTAGGCGGCAGCACTT  >  NC_002947/484557‑484841
                                                                                                                                          |                                                                                                                                                  
taCCCGCACGCCGGTGTACCGGCTCTCCCCTTTTTGGATGGCCTGAGCTTAAGCGTAGTTGGTTATCTGCAAGGTAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGTGCGCCGCGCGGGCtt                                                                                                                                         <  1:661688/149‑2 (MQ=255)
 aCCCGCACGCCGGTGTACCGGCTCTCCCCTTTTTGGATGGCCTGAGCTTAAGCGTAGTTGGTTATCTGCAAGGTAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGc                                                                                                                                        <  1:7607/150‑1 (MQ=255)
          ccGGTGTACCGGCTCTCCCCTTTTTGGATGGCCTGAGCTTAAGCGTAGTTGGTTATCTGCAAGGTAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGaaaa                                                                                                                               <  2:792064/150‑1 (MQ=255)
          ccGGTGTACCGGCTCTCCCCTTTTTGGATGGCCTGAGCTTAAGCGTAGTTGGTTATCTGCAAGGTAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGaaaa                                                                                                                               <  1:249855/150‑1 (MQ=255)
                       tctcCCCTTTTTGGATGGCCTGAGCTTAAGCGTAGTTGGTTATCTGCAAGGTAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCCAGCCCGCGCGTCGCACGGGCTGCAGGGGAAAATAAAAAATTATTc                                                                                                                  <  1:213941/150‑1 (MQ=255)
                           cccTTTTTGGATGGCCTGAGCTTAAGCGTAGTTGGTTATCTGCAAGGTAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCg                                                                                                              >  1:487803/1‑150 (MQ=255)
                                          cTGAGCTTAAGCGTAGTTGGTTATCTGCAAGGTAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTAAGATAAGCCGCAAGCCCGCGCGGCTCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGtc                                                                                               >  2:678574/1‑150 (MQ=255)
                                                ttAAGCGTAGTTGGTTATCTGCAAGGTAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGcc                                                                                         >  1:553092/1‑150 (MQ=255)
                                                ttAAGCGTAGTTGGTTATCTGCAAGGTAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGcc                                                                                         >  1:916626/1‑150 (MQ=255)
                                                    gCGTAGTTGGTTATCTGCAAGGTAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTgg                                                                                                     <  1:398073/134‑1 (MQ=255)
                                                    gCGTAGTTGGTTATCTGCAAGGTAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTgg                                                                                                     >  2:398073/1‑134 (MQ=255)
                                                    gCGTAGTTGGTTATCTGCAAGGTAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGt                                                                                     <  2:916626/150‑1 (MQ=255)
                                                       tAGTTGGTTATCTGCAAGGTAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAgg                                                                                  <  1:1118574/150‑1 (MQ=255)
                                                          ttGGTTATCTGCAAGGTAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCt                                                                                                       >  1:731456/1‑126 (MQ=255)
                                                          ttGGTTATCTGCAAGGTAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCt                                                                                                       <  2:731456/126‑1 (MQ=255)
                                                             gTTATCTGCAAGGTAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAGGTcgcgc                                                                            <  1:295219/150‑1 (MQ=255)
                                                                   tGCAAGGTAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAGGTCGCGCGCcaca                                                                      >  2:176385/1‑150 (MQ=255)
                                                                   tGCAAGGTAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAGGTCGCGCGCcaca                                                                      <  2:139770/150‑1 (MQ=255)
                                                                   tGCAAGGTAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCACGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGGAGGTCGCGCGCcaca                                                                      >  2:70558/1‑150 (MQ=255)
                                                                        ggTAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCg                                                                                                  <  1:613980/117‑1 (MQ=255)
                                                                        ggTAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCg                                                                                                  >  2:613980/1‑117 (MQ=255)
                                                                          tAANGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAGGTCGCGCGCCACAGTTCGaa                                                               >  2:699886/1‑150 (MQ=255)
                                                                          tAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAGGTCGCGCGCCACAGTTCGaa                                                               <  2:213450/150‑1 (MQ=255)
                                                                          tAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAGGTCGCGCGCCACAGTTCGaa                                                               <  1:766706/150‑1 (MQ=255)
                                                                           aaaGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCa                                                                                                                 <  1:949562/99‑1 (MQ=255)
                                                                           aaaGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCa                                                                                                                 >  2:949562/1‑99 (MQ=255)
                                                                           aaaGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAGGTCGCGCGCCACAGTTCGAAg                                                              <  1:414202/150‑1 (MQ=255)
                                                                                      aaaGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAGGTCGCGCGCCACAGTTCGAAGCCTCCGTCCAc                                                   <  2:487803/150‑1 (MQ=255)
                                                                                        aGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCACTGGGCGGTCTCGGCCGGGTAGGTCGCGCGCCACAGTTCGAAGCCTCCGTCCACGc                                                 >  2:1052235/1‑150 (MQ=255)
                                                                                                 ttACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAGGTCGCGCGCCACAGTTCGAAGTTTCCGTTCACGCCGTACACGt                                        >  2:526417/1‑150 (MQ=255)
                                                                                                  tACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAGGTCGCGCGCCACAGTTCGAAGCCTCCGTCCACGCTGTACACGTc                                       <  2:967846/150‑1 (MQ=255)
                                                                                                           ccGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAGGTCGCGCGCCACAGTTCGAAGCCTCCGTCCACGCTGTACACGTCGGAGAAAcc                              >  1:82202/1‑150 (MQ=255)
                                                                                                           ccGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAGGTCGCGCGCCACAGTTCGAAGCCTCCGTCCACGCTGTACACGTCGGAGAAAcc                              >  2:376987/1‑150 (MQ=255)
                                                                                                             gTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAgg                                                                                  <  2:1124655/96‑1 (MQ=255)
                                                                                                             gTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAgg                                                                                  >  1:1124655/1‑96 (MQ=255)
                                                                                                                             gCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAGGTCGCGCGCCACAGTTCGAAGCCTCCGTCCACGCTGTACACGTCGGAGAAACCCTGGCCTACCAGGTAggc            >  1:848802/1‑150 (MQ=255)
                                                                                                                                 gCCCGTGCGCCGCGCGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAGGTCGCGCGCCACAGTTCGAAGCCTCCGTCCACGCTGTACACGTCGGAGAAACCCTGGCCTACCAGGTa               <  1:91231/143‑1 (MQ=255)
                                                                                                                                 gCCCGTGCGCCGCGCGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAGGTCGCGCGCCACAGTTCGAAGCCTCCGTCCACGCTGTACACGTCGGAGAAACCCTGGCCTACCAGGTa               >  2:91231/1‑143 (MQ=255)
                                                                                                                                 gCCCGTGCGCCGCGCGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAGGTCGCGCGCCACAGTTCGAAGCCTCCGTCCACGCTGTACACGTCGGAGAAACCCTGGCCTACCAGGTAGGCGgca        >  2:479846/1‑150 (MQ=255)
                                                                                                                                 gCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAGGTCGCGCGCCACAGTTCGAAGCCTCCGTCCACGCTGTACACGTCGGAGAAACCCTGGCCTACCAGGTAGGCGgca        <  2:82202/150‑1 (MQ=255)
                                                                                                                                 gCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAGGTCGCGCGCCACAGTTCGAAGCCTCCGTCCACGCTGTACACGTCGGAGAAACCCTGGCCTACCAGGTAGGCGgca        <  1:37485/150‑1 (MQ=255)
                                                                                                                                      cgcgGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAGGTCGCGCGCCACAGTTCGAAGCCTCCGTCCACGCTGTACACGTCGGAGAAACCCTGGCCTACCAGGTAGGCGGCAGCACt   >  2:887536/1‑150 (MQ=255)
                                                                                                                                      cgcgGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAGGTCGCGCGCCACAGTTCGAAGCCTCCGTCCACGCTGTACACGTCGGAGAAACCCTGGCCTACCAGGTAGGCGGCAGCACt   <  1:699886/150‑1 (MQ=255)
                                                                                                                                       gcggcgCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAGGTCGCGCGCCACAGTTCGAAGCCTCCGTCCACGCTGTACACGTCGGAGAAACCCTGGCCTACCAGGTAGGCGGCAGCACtt  >  2:732040/1‑150 (MQ=255)
                                                                                                                                          |                                                                                                                                                  
GACCCGCACGCCGGTGTACCGGCTCTCCCCTTTTTGGATGGCCTGAGCTTAAGCGTAGTTGGTTATCTGCAAGGTAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAGGTCGCGCGCCACAGTTCGAAGCCTCCGTCCACGCTGTACACGTCGGAGAAACCCTGGCCTACCAGGTAGGCGGCAGCACTT  >  NC_002947/484557‑484841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑