Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,638,161 G→C 8.2% S215T (AGC→ACC)  PP_4949 → TldD/PmbA family peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,638,1610GC 8.2% 145.5 / 5.2 49S215T (AGC→ACC) PP_4949TldD/PmbA family peptidase
Reads supporting (aligned to +/- strand):  ref base G (20/25);  new base C (2/2);  total (22/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.96e-01

GCCATGCCCAGCGCTTCCTGTTCCCGGGCCTGGGGGTGACCGCCAGCGACGGCCAGGACAGCCAAAGCCGCAGCCTGGGCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGACCTGCTGTTGATGCCGGACCAGATGATGCTGCAGATTCACGAATCCATCGGCCACCCGCTGGAGATGGACCGCATTCTCGGCGAC  >  NC_002947/5638014‑5638309
                                                                                                                                                   |                                                                                                                                                    
gcCATGCCCAGCGCTTCCTGTTCCCGGGCCTGGGGGTGACCGCCAGCGACGGCCAGGACAGCCAAAGCCGCAGCCTGGGCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGcc                                                                                                                                                    <  2:526739/150‑1 (MQ=255)
gcCATGCCCAGCGCTTCCTGTTCCCGGGCCTGGGGGTGACCGCCAGCGACGGCCAGGACAGCCAAAGCCGCAGCCTGGGCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGAGCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGcc                                                                                                                                                    >  2:99752/1‑150 (MQ=255)
 ccATGCCCAGCGCTTCCTGTTCCCGGGCCTGGGGGTGACCGCCAGCGAAGGCCAGGACAGCCAAAGCCGCAGCCTGGGCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCccgccg                                                                                                                                                   >  2:214109/1‑150 (MQ=255)
       ccAGCGCTTCCTGTTCCCGGGCCTGGGGGTGACCGCCCGCGACGGCCAGGACAGCCAAAGCCGCAGCCTGGGCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGc                                                                                                                                             <  1:789843/150‑1 (MQ=255)
          gcgcTTCCTGTTCCCGGGCCTGGGGGTGACCGCCAGCGACGGCCAGGACAGCCAAAGCCGCAGCCTGGGCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCga                                                                                                                                          <  1:48833/150‑1 (MQ=255)
                        tgggCATGGGGGTGACCGCCAGCGACGGCCAGGACAGCCAAAGCCGCAGCCTGGGCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAAc                                                                                                                            <  2:83837/149‑1 (MQ=255)
                                     gACCGCCAGCGACGGCCAGGACAGCCAAAGCCGCAGCCTGGGCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCAcc                                                                                                               >  2:736208/1‑150 (MQ=255)
                                        cGCCAGCGACGGCCAGGACAGCCAACGCCGCCGCCCGGCGAACTGCTGGGCGAGGTAACAGGGTGGTTTGGGGAGCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCTTAATAATCACTGCAACTGCTGCTGGCACCGaa                                                                                                            >  1:192976/1‑150 (MQ=255)
                                        cGCCAGCGACGGCCAGGACAGCCAAAGCCGCAGCCTGGGCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGaa                                                                                                            <  1:481267/150‑1 (MQ=255)
                                         gCCAGCGACGGCCAGGACAGCCAAAGCCGCAGCCTGGGCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAac                                                                                                           <  2:253294/150‑1 (MQ=255)
                                                    ccAGGACAGCCAAAGCCGCAGCCTGGGCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCACCCGGCTGGCCgc                                                                                                                                          >  2:56721/1‑107 (MQ=255)
                                                    ccAGGACAGCCAAAGCCGCAGCCTGGGCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCACCCGGCTGGCCgc                                                                                                                                          <  1:56721/108‑2 (MQ=255)
                                                     cAGGACAGCCAAAGCCGCAGCCTGGGCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGc                                                                                               >  2:968529/1‑150 (MQ=255)
                                                       ggACAGCCAAAGCCGCAGCCTGGGCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGacc                                                                                             <  2:74947/150‑1 (MQ=255)
                                                           aGCCAAAGCCGCAGCCTGGGCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCAc                                                                                                                                  >  2:86590/1‑109 (MQ=255)
                                                           aGCCAAAGCCGCAGCCTGGGCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCAc                                                                                                                                  <  1:86590/109‑1 (MQ=255)
                                                           aGCCAAAGCCGCAGCCTGGGCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCcgcgc                                                                                         <  1:945726/150‑1 (MQ=255)
                                                             ccAAAGCCGCAGCCTGGGCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGGTCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGACGAAAAAGCACTGCAACTGCTGATGGCACCGAACACCCCCAGTGGCCCGCGCGa                                                                                       >  2:578879/1‑150 (MQ=255)
                                                             ccAAAGCCGCAGCCTGGGCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGa                                                                                       >  1:187132/1‑150 (MQ=255)
                                                              cAAAGCCGCAGCCTGGGCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGAc                                                                                      <  1:497612/150‑1 (MQ=255)
                                                              cAAAGCCGCAGCCTGGGCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGAc                                                                                      <  1:464220/150‑1 (MQ=255)
                                                                    cGCAGCCTGGGCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCg                                                                                                                                           >  1:553709/1‑91 (MQ=255)
                                                                    cGCAGCCTGGGCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCg                                                                                                                                           <  2:553709/91‑1 (MQ=255)
                                                                            gggCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGACCTGCTGt                                                                               <  1:621553/143‑1 (MQ=255)
                                                                            gggCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGACCTGCTGt                                                                               >  2:621553/1‑143 (MQ=255)
                                                                                 gcgACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGc                                                                                                                               <  1:691854/90‑1 (MQ=255)
                                                                                 gcgACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGc                                                                                                                               <  1:690399/90‑1 (MQ=255)
                                                                                 gcgACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGc                                                                                                                               >  2:691854/1‑90 (MQ=255)
                                                                                 gcgACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGc                                                                                                                               >  2:690399/1‑90 (MQ=255)
                                                                                    aCAACTTCGGCCAACAGGGTGGTTTCGAGGTCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCTCGCGACCTGCTGTTGATGCCGGACCAga                                                                <  1:578879/150‑1 (MQ=255)
                                                                                     cAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCAGGGTGGCCGAAGAAGAACTGCACCTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGACCTGCTGTTGATGCCGGACCAgat                                                               <  2:499748/150‑1 (MQ=255)
                                                                                         ttCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGAcc                                                                                     >  1:1029211/1‑124 (MQ=255)
                                                                                         ttCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGAcc                                                                                     <  2:1029211/124‑1 (MQ=255)
                                                                                                aaCAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGACCTGCTGTTGATGCCGGACCAGATGATTCTGCAgc                                                    <  2:187132/150‑2 (MQ=255)
                                                                                                        ggtTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGACCTGCTGTTGATGCCGGACCAGATGATGCTGCAGATTCACGaa                                            <  1:956754/150‑1 (MQ=255)
                                                                                                         gtTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGACCTGCTGTTGATGCCGGACCAGATGATGCTGCAGATTCACGAAt                                           >  2:180900/1‑150 (MQ=255)
                                                                                                                gaTCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTgg                                                                                                                   <  2:875227/71‑1 (MQ=255)
                                                                                                                gaTCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTgg                                                                                                                   >  1:875227/1‑71 (MQ=255)
                                                                                                                gaTCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGACCTGCTGTTGATGCCGGACCAGATGATGCTGCAGATTCACGAATCCATCgg                                    >  1:321555/1‑150 (MQ=255)
                                                                                                                gaTCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGACGCAGTATCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGACCTGCTGTTGATGCCGGACCAGATGATGCTGCAGATTCACGAATCCATCgg                                    >  1:475989/1‑150 (MQ=255)
                                                                                                                    atcGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGACCTGCTGTTGATGCCGGACCAGCTGATGCTGCAGATTCACGAATCCATCGGCCAc                                >  2:196716/1‑150 (MQ=255)
                                                                                                                    atcGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGACCTGCTGTTGATGCCGGACCAGATGATGCTGCAGATTCACGAATCCATCGGCCAc                                >  2:57818/1‑150 (MQ=255)
                                                                                                                          cgcTGCGGCCTGGTCGGCGCGCCCAGCGGGGTGGCCGAAGAAGCACTGCAACGGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGACCTGCTGTTGATGCCGGACCAGATGATGCTGCAGATTCACGAATCCATCGGCCACCCGCTg                          <  2:192976/150‑1 (MQ=255)
                                                                                                                            cTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGACCTGCTGTTGATGCCGGACCAGATGATGCTGCAGATTCACGAATCCACCGGCCACCCGCTGga                        <  2:1104374/150‑1 (MQ=255)
                                                                                                                                ggCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGACCTGCTGTTGATGCCGGACCAGATGATGCTGCAGATTCACGAATCCATCGGCCACCCGCTGGAGATg                    >  2:530069/1‑150 (MQ=255)
                                                                                                                                    tGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGACCTGCTGTTGATGCCGGACCAGATGATGCTGCAGATTCACGa                                             >  2:617504/1‑121 (MQ=255)
                                                                                                                                    tGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGACCTGCTGTTGATGCCGGACCAGATGATGCTGCAGATTCACGa                                             <  1:617504/121‑1 (MQ=255)
                                                                                                                                         tcttcgGCCACCCGGCTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGACCTGCTGTTGATGCCGGACCAGATGAtgc                                                          >  2:139338/5‑103 (MQ=255)
                                                                                                                                         tcttcgGCCACCCGGCTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGACCTGCTGTTGATGCCGGACCAGATGAtgc                                                          <  1:139338/99‑1 (MQ=255)
                                                                                                                                                  aGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGACCTGCTGTTGATGCCGGACCAGATGATGCTGCAGATTCACGAATCCATCGGCCACCCGCTGGAGATGGACCGCATTCTCGGcgac  <  1:99752/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCCATGCCCAGCGCTTCCTGTTCCCGGGCCTGGGGGTGACCGCCAGCGACGGCCAGGACAGCCAAAGCCGCAGCCTGGGCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGACCTGCTGTTGATGCCGGACCAGATGATGCTGCAGATTCACGAATCCATCGGCCACCCGCTGGAGATGGACCGCATTCTCGGCGAC  >  NC_002947/5638014‑5638309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑