Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,638,166 G→C 8.5% V217L (GTG→CTG)  PP_4949 → TldD/PmbA family peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,638,1660GC 8.5% 143.8 / 5.2 47V217L (GTG→CTG) PP_4949TldD/PmbA family peptidase
Reads supporting (aligned to +/- strand):  ref base G (19/24);  new base C (2/2);  total (21/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.73e-01

CCAGCGCTTCCTGTTCCCGGGCCTGGGGGTGACCGCCAGCGACGGCCAGGACAGCCAAAGCCGCAGCCTGGGCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGACCTGCTGTTGATGCCGGACCAGATGATGCTGCAGATTCACGAATCCATCGGCCACCCGCTGGAGATGGACCGCATTCTCGGCGAC  >  NC_002947/5638021‑5638309
                                                                                                                                                 |                                                                                                                                               
ccAGCGCTTCCTGTTCCCGGGCCTGGGGGTGACCGCCCGCGACGGCCAGGACAGCCAAAGCCGCAGCCTGGGCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGc                                                                                                                                             <  1:789843/150‑1 (MQ=255)
   gcgcTTCCTGTTCCCGGGCCTGGGGGTGACCGCCAGCGACGGCCAGGACAGCCAAAGCCGCAGCCTGGGCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCga                                                                                                                                          <  1:48833/150‑1 (MQ=255)
                 tgggCATGGGGGTGACCGCCAGCGACGGCCAGGACAGCCAAAGCCGCAGCCTGGGCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAAc                                                                                                                            <  2:83837/149‑1 (MQ=255)
                              gACCGCCAGCGACGGCCAGGACAGCCAAAGCCGCAGCCTGGGCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCAcc                                                                                                               >  2:736208/1‑150 (MQ=255)
                                 cGCCAGCGACGGCCAGGACAGCCAACGCCGCCGCCCGGCGAACTGCTGGGCGAGGTAACAGGGTGGTTTGGGGAGCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCTTAATAATCACTGCAACTGCTGCTGGCACCGaa                                                                                                            >  1:192976/1‑150 (MQ=255)
                                 cGCCAGCGACGGCCAGGACAGCCAAAGCCGCAGCCTGGGCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGaa                                                                                                            <  1:481267/150‑1 (MQ=255)
                                  gCCAGCGACGGCCAGGACAGCCAAAGCCGCAGCCTGGGCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAac                                                                                                           <  2:253294/150‑1 (MQ=255)
                                             ccAGGACAGCCAAAGCCGCAGCCTGGGCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCACCCGGCTGGCCgc                                                                                                                                          >  2:56721/1‑107 (MQ=255)
                                             ccAGGACAGCCAAAGCCGCAGCCTGGGCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCACCCGGCTGGCCgc                                                                                                                                          <  1:56721/108‑2 (MQ=255)
                                              cAGGACAGCCAAAGCCGCAGCCTGGGCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGc                                                                                               >  2:968529/1‑150 (MQ=255)
                                                ggACAGCCAAAGCCGCAGCCTGGGCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGacc                                                                                             <  2:74947/150‑1 (MQ=255)
                                                    aGCCAAAGCCGCAGCCTGGGCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCAc                                                                                                                                  >  2:86590/1‑109 (MQ=255)
                                                    aGCCAAAGCCGCAGCCTGGGCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCAc                                                                                                                                  <  1:86590/109‑1 (MQ=255)
                                                    aGCCAAAGCCGCAGCCTGGGCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCcgcgc                                                                                         <  1:945726/150‑1 (MQ=255)
                                                      ccAAAGCCGCAGCCTGGGCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGGTCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGACGAAAAAGCACTGCAACTGCTGATGGCACCGAACACCCCCAGTGGCCCGCGCGa                                                                                       >  2:578879/1‑150 (MQ=255)
                                                      ccAAAGCCGCAGCCTGGGCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGa                                                                                       >  1:187132/1‑150 (MQ=255)
                                                       cAAAGCCGCAGCCTGGGCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGAc                                                                                      <  1:497612/150‑1 (MQ=255)
                                                       cAAAGCCGCAGCCTGGGCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGAc                                                                                      <  1:464220/150‑1 (MQ=255)
                                                             cGCAGCCTGGGCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCg                                                                                                                                           >  1:553709/1‑91 (MQ=255)
                                                             cGCAGCCTGGGCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCg                                                                                                                                           <  2:553709/91‑1 (MQ=255)
                                                                     gggCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGACCTGCTGt                                                                               <  1:621553/143‑1 (MQ=255)
                                                                     gggCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGACCTGCTGt                                                                               >  2:621553/1‑143 (MQ=255)
                                                                          gcgACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGc                                                                                                                               <  1:691854/90‑1 (MQ=255)
                                                                          gcgACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGc                                                                                                                               <  1:690399/90‑1 (MQ=255)
                                                                          gcgACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGc                                                                                                                               >  2:691854/1‑90 (MQ=255)
                                                                          gcgACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGc                                                                                                                               >  2:690399/1‑90 (MQ=255)
                                                                             aCAACTTCGGCCAACAGGGTGGTTTCGAGGTCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCTCGCGACCTGCTGTTGATGCCGGACCAga                                                                <  1:578879/150‑1 (MQ=255)
                                                                              cAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCAGGGTGGCCGAAGAAGAACTGCACCTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGACCTGCTGTTGATGCCGGACCAgat                                                               <  2:499748/150‑1 (MQ=255)
                                                                                  ttCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGAcc                                                                                     <  2:1029211/124‑1 (MQ=255)
                                                                                  ttCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGAcc                                                                                     >  1:1029211/1‑124 (MQ=255)
                                                                                         aaCAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGACCTGCTGTTGATGCCGGACCAGATGATTCTGCAgc                                                    <  2:187132/150‑2 (MQ=255)
                                                                                                 ggtTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGACCTGCTGTTGATGCCGGACCAGATGATGCTGCAGATTCACGaa                                            <  1:956754/150‑1 (MQ=255)
                                                                                                  gtTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGACCTGCTGTTGATGCCGGACCAGATGATGCTGCAGATTCACGAAt                                           >  2:180900/1‑150 (MQ=255)
                                                                                                         gaTCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTgg                                                                                                                   <  2:875227/71‑1 (MQ=255)
                                                                                                         gaTCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTgg                                                                                                                   >  1:875227/1‑71 (MQ=255)
                                                                                                         gaTCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGACCTGCTGTTGATGCCGGACCAGATGATGCTGCAGATTCACGAATCCATCgg                                    >  1:321555/1‑150 (MQ=255)
                                                                                                         gaTCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGACGCAGTATCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGACCTGCTGTTGATGCCGGACCAGATGATGCTGCAGATTCACGAATCCATCgg                                    >  1:475989/1‑150 (MQ=255)
                                                                                                             atcGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGACCTGCTGTTGATGCCGGACCAGCTGATGCTGCAGATTCACGAATCCATCGGCCAc                                >  2:196716/1‑150 (MQ=255)
                                                                                                             atcGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGACCTGCTGTTGATGCCGGACCAGATGATGCTGCAGATTCACGAATCCATCGGCCAc                                >  2:57818/1‑150 (MQ=255)
                                                                                                                   cgcTGCGGCCTGGTCGGCGCGCCCAGCGGGGTGGCCGAAGAAGCACTGCAACGGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGACCTGCTGTTGATGCCGGACCAGATGATGCTGCAGATTCACGAATCCATCGGCCACCCGCTg                          <  2:192976/150‑1 (MQ=255)
                                                                                                                     cTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGACCTGCTGTTGATGCCGGACCAGATGATGCTGCAGATTCACGAATCCACCGGCCACCCGCTGga                        <  2:1104374/150‑1 (MQ=255)
                                                                                                                         ggCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGACCTGCTGTTGATGCCGGACCAGATGATGCTGCAGATTCACGAATCCATCGGCCACCCGCTGGAGATg                    >  2:530069/1‑150 (MQ=255)
                                                                                                                             tGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGACCTGCTGTTGATGCCGGACCAGATGATGCTGCAGATTCACGa                                             >  2:617504/1‑121 (MQ=255)
                                                                                                                             tGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGACCTGCTGTTGATGCCGGACCAGATGATGCTGCAGATTCACGa                                             <  1:617504/121‑1 (MQ=255)
                                                                                                                                  tcttcgGCCACCCGGCTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGACCTGCTGTTGATGCCGGACCAGATGAtgc                                                          >  2:139338/5‑103 (MQ=255)
                                                                                                                                  tcttcgGCCACCCGGCTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGACCTGCTGTTGATGCCGGACCAGATGAtgc                                                          <  1:139338/99‑1 (MQ=255)
                                                                                                                                           aGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGACCTGCTGTTGATGCCGGACCAGATGATGCTGCAGATTCACGAATCCATCGGCCACCCGCTGGAGATGGACCGCATTCTCGGcgac  <  1:99752/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CCAGCGCTTCCTGTTCCCGGGCCTGGGGGTGACCGCCAGCGACGGCCAGGACAGCCAAAGCCGCAGCCTGGGCCGCGACAACTTCGGCCAACAGGGTGGTTTCGAGATCATCGAGCGCTGCGGCCTGGTCGGCGCGGCCAGCCGGGTGGCCGAAGAAGCACTGCAACTGCTGCTGGCACCGAACACCCCCAGTGGCCCGCGCGACCTGCTGTTGATGCCGGACCAGATGATGCTGCAGATTCACGAATCCATCGGCCACCCGCTGGAGATGGACCGCATTCTCGGCGAC  >  NC_002947/5638021‑5638309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑