Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,670,029 C→T 8.5% A152V (GCC→GTC)  metF → 5,10‑methylenetetrahydrofolate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,670,0290CT 8.5% 124.8 / 3.3 47A152V (GCC→GTC) metF5,10‑methylenetetrahydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base C (20/23);  new base T (2/2);  total (22/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.76e-01

GCATCAAACGCATTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTACTTCTTCAACGCCGACAGCTACTTCTACTTCGTCGAGCGT  >  NC_002947/5669891‑5670168
                                                                                                                                          |                                                                                                                                           
gCATCAAACGCATTGTCGTGCTGCGTGGTGACCTGACGTCGGGAATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGCAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGaaa                                                                                                                                  <  2:204199/150‑1 (MQ=255)
gCATCAAACGCAGTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGaaa                                                                                                                                  <  1:717076/150‑1 (MQ=255)
  aTCAAACGCATTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATg                                                                                                                                >  1:815734/1‑150 (MQ=255)
     aaaCGCATTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCAc                                                                                                                             >  1:125649/1‑150 (MQ=255)
               tCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAg                                                                                                                       >  2:898119/1‑146 (MQ=255)
               tCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGATATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAg                                                                                                                       <  1:898119/146‑1 (MQ=255)
                  cgcTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCa                                                                                                                >  1:267073/1‑150 (MQ=255)
                   gcTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCaa                                                                                                               <  1:1029459/150‑1 (MQ=255)
                                 tGCCGTCGGGCATGGGCATGGCCCGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGaac                                                                                                 >  2:550579/1‑148 (MQ=255)
                                  gCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCt                                                                                                <  2:339753/150‑1 (MQ=255)
                                   ccGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTg                                                                                               <  1:442561/150‑1 (MQ=255)
                                        gggcatgggcatGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCaa                                                                                          <  2:1064899/150‑1 (MQ=255)
                                          gcatgggcatggCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACt                                                                                        >  2:212324/1‑150 (MQ=255)
                                           catgggcatggCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACtt                                                                                       <  2:267073/150‑1 (MQ=255)
                                                 catggCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGcc                                                                                 >  2:786868/1‑149 (MQ=255)
                                                    ggCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACaa                                                                              <  1:829142/150‑1 (MQ=255)
                                                           ggtgAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAg                                                                       >  2:201118/1‑150 (MQ=255)
                                                            gtgAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAgg                                                                      >  1:567203/1‑150 (MQ=255)
                                                                  tGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCGGGCTCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTg                                                                <  2:125649/150‑1 (MQ=255)
                                                                           ccAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAgcg                                                       <  2:891050/150‑1 (MQ=255)
                                                                              gTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCg                                                                   <  1:633026/135‑1 (MQ=255)
                                                                              gTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCg                                                                   >  2:633026/1‑135 (MQ=255)
                                                                                  acTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCAc                                                <  2:172129/149‑1 (MQ=255)
                                                                                     ggTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCa                                             <  2:815734/150‑1 (MQ=255)
                                                                                           gTTCATGAGCCAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTActt                                       >  2:325757/1‑150 (MQ=255)
                                                                                            ttCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTTcttt                                      >  1:165387/1‑149 (MQ=255)
                                                                                            ttCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTActtc                                      >  2:908446/1‑150 (MQ=255)
                                                                                            ttCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTActtc                                      <  2:833669/150‑1 (MQ=255)
                                                                                                tCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGcc                                                              >  2:757226/1‑122 (MQ=255)
                                                                                                tCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGcc                                                              <  1:757226/122‑1 (MQ=255)
                                                                                                             cGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTACTTCTTCAACGCCGa                           >  1:487098/1‑144 (MQ=255)
                                                                                                             cGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTACTTCTTCAACGCCGa                           <  2:487098/144‑1 (MQ=255)
                                                                                                                tGATCACTTCCACCTGGAAGTGGCTGTCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTAc                                         <  2:802842/127‑1 (MQ=255)
                                                                                                                tGATCACTTCCACCTGGAAGTGGCTGTCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTAc                                         <  2:802906/127‑1 (MQ=255)
                                                                                                                tGATCACTTCCACCTGGAAGTGGCTGTCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTAc                                         >  1:802906/1‑127 (MQ=255)
                                                                                                                tGATCACTTCCACCTGGAAGTGGCTGTCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTAc                                         >  1:802842/1‑127 (MQ=255)
                                                                                                                 gATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTACTTCTTCAACGCCGACAGctacttc                 <  1:908446/150‑1 (MQ=255)
                                                                                                                  aTCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTACTTCTTCAACGCCGACAGctacttct                >  2:288548/1‑150 (MQ=255)
                                                                                                                   ccACTTCCAGGTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTACTTCTTCAACGCCGACAGctacttcta               >  2:73153/2‑150 (MQ=255)
                                                                                                                    cACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTAc                                         <  2:368122/123‑1 (MQ=255)
                                                                                                                    cACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTAc                                         >  1:368122/1‑123 (MQ=255)
                                                                                                                    cACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTACTTCTTCAACGCCGACAGctacttctac              <  2:927530/150‑1 (MQ=255)
                                                                                                                       ttCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAg                                            >  1:232093/1‑117 (MQ=255)
                                                                                                                       ttCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAg                                            <  2:232093/117‑1 (MQ=255)
                                                                                                                              tGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGc                                                               <  1:251520/91‑1 (MQ=255)
                                                                                                                              tGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGc                                                               >  2:251520/1‑91 (MQ=255)
                                                                                                                                gAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTACTTCTTCAACGCCGACAGCTACTTCTACTTCGTCGAGCGt  <  1:288548/150‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                           
GCATCAAACGCATTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTACTTCTTCAACGCCGACAGCTACTTCTACTTCGTCGAGCGT  >  NC_002947/5669891‑5670168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑