| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,726,657 | G→A | 7.3% | P122L (CCG→CTG) | opgH ← | glucans biosynthesis glucosyltransferase H |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,726,657 | 0 | G | A | 7.3% | 154.2 / 2.9 | 55 | P122L (CCG→CTG) | opgH | glucans biosynthesis glucosyltransferase H |
| Reads supporting (aligned to +/- strand): ref base G (26/25); new base A (2/2); total (28/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.72e-01 | |||||||||||
ACCAGCCCTGGTACGGCAGGATGCCTTTCATGTACCAGCCAGCGACGATGGTCTGGCCGATCATCAGCGCCAGCAGAATGTAACGGCGGATAGAGCCGACCGTGCGCCAGCGGGCCGGCGGCAGCTCGCGCTT‑GGGCGGCTGCGGGGCATTGGGGCGGCCGGTCATGCGTCGCCACATGCGGATCAGCACATTGGTGCGCCATGGCTCGGGCACGACCTTGGTGCGTTTGATCGGCGGGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGA > NC_002947/5726520‑5726798 | aCCAGCCCTGGTACGGCAGGATGCCTTTCATGTACCAGCCAGCGACGATGGTCTGGCCGATCATCAGCGCCAGCAGAATGTAACGGCGGATAGAGCCGACCGTGCGCCAGCGGGCCGGCGGCAGCTCGCGCTT‑GGGCGGCTGCGGGGCAt > 1:401876/1‑150 (MQ=255) tGGTACGGCAGGATGCCTTTCATGTACCAGCCAGCGACGATGGTCTGGCCGATCATCAGCGCCAGCAGAATGTAACGGCGGATAGAGCCGACCGTGCGCCAGCGGGCCGGCGGCAGCTCGCGCTT‑GGGCGGCTGCgggg > 2:858399/1‑139 (MQ=255) tGGTACGGCAGGATGCCTTTCATGTACCAGCCAGCGACGATGGTCTGGCCGATCATCAGCGCCAGCAGAATGTAACGGCGGATAGAGCCGACCGTGCGCCAGCGGGCCGGCGGCAGCTCGCGCTT‑GGGCGGCTGCgggg < 1:858399/139‑1 (MQ=255) gCAGGATGCCTTTCATGTACCAGCCAGCGACGATGGTCTGGCCGATCATCAGCGCCAGCAGAATGTAACGGCGGATAGAGCCGACCGTGCGCCAGCGGGCCGGCGGCAGCTCGCGCTT‑Gggcg > 2:916818/1‑123 (MQ=255) gCAGGATGCCTTTCATGTACCAGCCAGCGACGATGGTCTGGCCGATCATCAGCGCCAGCAGAATGTAACGGCGGATAGAGCCGACCGTGCGCCAGCGGGCCGGCGGCAGCTCGCGCTT‑Gggcg < 1:916818/123‑1 (MQ=255) gCAGGATGCCTTTCATGTACCAGCCAGCGACGATGGTCTGGCCGATCATCAGCGCCAGCAGAATGTAACGGCGGATAGAGCCGACCGTGCGCCAGCGGGCCGGCGGCAGCTCGCGCTT‑GGGCGGCTGCGGGGCATTGGGGCGGCCGGTCa > 1:671306/1‑150 (MQ=255) tttCATGTACCGGCCAGCGACGATGGTCTGGCCGATCATCAGCGCCAGCAGAATGTAACGGCGGATAGAGCCGACCGTGCGCCAGCGGGCCGGCGGCAGCTCGCGCTT‑GGGCGGCTGCGGGGCATTGGGGCGGCCGGTCATGCGTCGCca < 2:56954/150‑1 (MQ=255) tttCATGTACCAGCCAGCGACGATGGTCTGGCCGATCATCAGCGCCAGCAGAATGTAACGGCGGATAGAGCCGACCGTGCGCCAGCGGGCCGGCGGCAGCTCGCGCTT‑GGGCGGCTGCGGGGCATTGGGGCGGCCGGTCATGCGTCGCca > 1:149311/1‑150 (MQ=255) tttCATGTACCAGCCAGCGACGATGGTCTGGCCGATCATCAGCGCCAGCAGAATGTAACGGCGGATAGAGCCGACCGTCTGCTAGCGGGCCGGCGGCAGCTCGCGCTT‑GGGCGGCTGCGGGGCATTGGGGCGGCCGGTCATGCGTCGCca > 1:912358/1‑150 (MQ=255) tGTACCAGCCAGCGACGATGGTCTGGCCGATCATCAGCGCCAGCAGAATGTAACGGCGGATAGAGCCGACCGTGCGCCAGCGGGCCGGCGGCAGCTCGCGCTT‑GGGCGGCTGCGGGGCATTGGGGCGGCCGGTCATGCGTCGCCACATGc < 1:590054/150‑1 (MQ=255) tACCAGCCAGCGACGATGGTCTGGCCGATCATCAGCGCCAGCAGAATGTAACGGCGGATAGAGCCGACCGTGCGCCAGCGGGCCGGCGGCAGCTCGCGCTT‑GGGCGGCTGCGGGGCATTGGGGCGGCCGGTCATGCGTCGCCACATGCgg > 2:822597/1‑150 (MQ=255) aTGGTCTGGCCGATCATCAGCGCCAGCAGAATGTAACGGCGGATAGAGCCGACCGTGCGCCAGCGGGCCGGCGGCAGCTCGCGCTT‑GGGCGGCTGCGGGGCATTGGGGCGGCCGGTCATGCGTCGCCACATGCGGATCAGCACATTGGTg > 1:210402/1‑150 (MQ=255) aTGGTCTGGCCGATCATCAGCGCCAGCAGAATGTAACGGCGGATAGAGCCGACCGTGCGCCAGCGGGCCGGCGGCAGCTCGCGCTT‑GGGCGGCTGCGGGGCATTGGGGCGGCCGGTCATGCGTCGCCACATGCGGATCAGCACATTGGTg < 2:37666/150‑1 (MQ=255) tGGCCGATCATCAGCGCCAGCAGAATGTAACGGCGGATAGAGCCGACCGTGCGCCAGCGGGCCGGCGGCAGCTCGCGCTT‑GGGCGGCTGCGGGGCATTGGGGCGGCCGGTCATGCGt < 1:44303/117‑1 (MQ=255) tGGCCGATCATCAGCGCCAGCAGAATGTAACGGCGGATAGAGCCGACCGTGCGCCAGCGGGCCGGCGGCAGCTCGCGCTT‑GGGCGGCTGCGGGGCATTGGGGCGGCCGGTCATGCGt > 2:44303/1‑117 (MQ=255) tGGCCGATCATCAGCGCCAGCAGAATGTAACGGCGGATAGAGCCGACCGTGCGCCAGCGGGCCGGCGGCAGCTCGCGCTT‑GGGCGGCTGCGGGGCATTGGGGCGGCCGGTCATGCGTCGCCACATGCGGATCAGCACATTg < 2:312876/141‑1 (MQ=255) tGGCCGATCATCAGCGCCAGCAGAATGTAACGGCGGATAGAGCCGACCGTGCGCCAGCGGGCCGGCGGCAGCTCGCGCTT‑GGGCGGCTGCGGGGCATTGGGGCGGCCGGTCATGCGTCGCCACATGCGGATCAGCACATTg > 1:312876/1‑141 (MQ=255) gggCCGATCATCAGCGACAGCAGAATGTAACGGCGGATAGAGCCGACCGTGCGCCAGCGGGCCGGCGGCAGCTCGCGCTT‑GGGCGGCTGCGGGGCATTGGGGCGGCCGGTCATGCGTCGCCACATGCGGATCAGCACATTGGTGCGCCAt < 1:109469/149‑1 (MQ=255) gCCGATCATCAGCGCCAGCAGAATGTAACGGCGGATATAAAGTACTGGGTTCTATCGGGCCGGCGGCAGCCCCCGGTTGGGGCGGATG‑GTTTCAGTGGGGCGGCCGGTCATGCGTCGCCACATGCGGATCAGAACATTGTTTCGCCAttg > 1:523907/1‑148 (MQ=255) gATCATCAGCGCCAGCAGAATGTAACGGCGGATAGAGCCGACCGTGCGCCAGCGGGCCGGCGGCAGCTCGCGCTT‑GGGCGGCTGCGGGGCATTGGGGCGGCCGGTCATGCGTCGCCACATGCGGATCAGCACATTGGTGCGCCATGGCTc > 2:225721/1‑150 (MQ=255) tcatcaGCGCCAGCAGAATGTAACGGCGGATAGAGCCGACCGTGCGCCAGCGGGCCGGCGGCAGCTCGCGCTT‑GGGCGGCTGCGGGGCATTGGGGCGGCCGGTCATGCGTCGCCACATGCGGATCAGCACATTGGTGCGCCATGGCTCgg < 2:452773/150‑1 (MQ=255) tcatcaGCGCCAGCAGAATGTAACGGCGGATAGAGCCGACCGTGCGCCAGCGGGCCGGCGGCAGCTCGCGCTT‑GGGCGGCTGCGGGGCATTGGGGCGGCCGGTCATGCGTCGCCACATGCGGATCAGCACATTGGTGCGCCATGGCTCgg < 1:813774/150‑1 (MQ=255) tcatcaGCGCCAGCAGAATGTAACGGCGGATAGAGCCGACCGTGCGCCAGCGGGCCGGCGGCAGCTCGCGCTT‑GGGCGGCTGCGGGGCATTGGGGCGGCCGGTCATGCGTCGCCACATGCGGATCAGCACATTGGTGCGCCATGGCTCgg < 2:557900/150‑1 (MQ=255) atcaGCGCCAGCAGAATGTAACGGCGGATAGAGCCGACCGTGCGCCAGCGGGCCGGCGGCAGCTCGCGCTT‑GGGCGGCTGCGGGGCATTGGGGCGGCCGGTCATGCGTCGCCACATGCGGATCAGCACATTGGTGCGCCATGGCTCGGGc > 1:846528/1‑150 (MQ=255) caGCGCCAGCAGAATGTAACGGCGGATAGAGCCGACCGTGCGCCAGCGGGCCGGCGGCAGCTCGCGCTT‑GGGCGGCTGCGGGGCATTGGGGCGGCCGGTCATGCGTCGCCACATGCGGATCAGCACATTGGTGCGCCATGGCTCGGGCAc > 1:871542/1‑150 (MQ=255) cagcagAATGTAACGGCGGATAGAGCCGACCGTGCGCCAGCGGGCCGGCGGCAGCTCGCGCTT‑GGGCGGCTGCGGGGCATTGGGGCGGCCGGTCATGCGTCGCCACATGCGGATCAGCACATTGGTGCGCCATGGCTCGGGCACGACCtt > 1:238714/1‑150 (MQ=255) cagcagAATGTAACGGCGGATAGAGCCGAAAGTGCGCTTGTGTTTCGTTTAATTGTCTTGCTT‑GGGCGGCTCTAGTGCATTGGGGCGGCCGGTCATGCGTCGCCACATGCGGATCAGCACATTGGTGCGCCATGGCTCGGGCACGACCtt > 1:193278/1‑150 (MQ=255) gAATGTAACGGCGGATAGAGCCGACCGTGCGCCAGCGGGCCGGCGGCAGCTCGCGCTT‑GGGCGGCTGCGGGGCATTGGGGCGGCCGGTCATGCGTCGCCACATGCGGATCAGCACATTGGTGCGCCATGGCTCGGGCACGACCTTGGt > 1:498576/1‑148 (MQ=255) gAATGTAACGGCGGATAGAGCCGACCGTGCGCCAGCGGGCCGGCGGCAGCTCGCGCTT‑GGGCGGCTGCGGGGCATTGGGGCGGCCGGTCATGCGTCGCCACATGCGGATCAGCACATTGGTGCGCCATGGCTCGGGCACGACCTTGGt < 2:498576/148‑1 (MQ=255) gAATGTAACGGCGGATAGAGCCGACCGTGCGCCAGCGGGCCGGCGGCAGCTCGCGCTT‑GGGCGGCTGCGGGGCATTGGGGCGGCCGGTCATGCGTCGCCACATGCGGATCAGCACATTGGTGCGCCATGGCTCGGGCACGACCTTGGTGc > 2:963974/1‑150 (MQ=255) gAATGTAACGGCGGATAGAGCCGACCGTGCGCAAGAATGCCGGCGGCAGCTCGCGCTT‑GGGCGGCTGCGGGGCATTGGGGCGGCCGGTCATGCGTCGCCACATGCGGATCAGCACATTGGTGCGCCATGGCTCGGGCACGACCTTGGTGc > 1:366647/1‑150 (MQ=255) aaTGTAACGGCGGATAGAGCCGACCGTGCGCCAGCGGGCCGGCGGCAGCTCGCGCTT‑GGGCGGCTGCGGGGCATTGGGGCGGCCGGTCATGCGTCGCCACATGCGGATCAGCACATTGGTGCGCCATGGCTCGGGCACGACCTTGGTGCg > 2:523367/1‑150 (MQ=255) aTGTAACGGCGGATAGAGCCGACCGTGCGCCAGCGGGCCGGCGGCAGCTCGCGCTT‑GGGCGGCTGCGGGGCATTGGGGCGGCCGGTCATGCGTCGCCACATGCGGATCAGCACATTGGTGCGCCATGGCTCGGGCACGACCTTGGTGCGt < 1:404820/150‑1 (MQ=255) gTAACGGCGGATAGAGCCGACCGTGCGCCAGCGGGCCGGCGGCAGCTCGCGCTT‑GGGCGGCTGCGGGGCATTGGGGCGGCCGGTCATGCGTCGCCACATGCGGATCAGCACATTGGTGCGCCATGGCTCGGGCACGACCTTGGTGCGttt < 1:822597/150‑1 (MQ=255) aCGGCGGATAGAGCCGACCGTGCGCCAGCGGGCCGGCGGCAGCTCGCGCTT‑GGGCAGCTGCgg > 1:1120466/1‑63 (MQ=255) aCGGCGGATAGAGCCGACCGTGCGCCAGCGGGCCGGCGGCAGCTCGCGCTT‑GGGCAGCTGCgg < 2:538492/63‑1 (MQ=255) aCGGCGGATAGAGCCGACCGTGCGCCAGCGGGCCGGCGGCAGCTCGCGCTT‑GGGCAGCTGCgg < 2:1120466/63‑1 (MQ=255) aCGGCGGATAGAGCCGACCGTGCGCCAGCGGGCCGGCGGCAGCTCGCGCTT‑GGGCAGCTGCgg > 1:538492/1‑63 (MQ=255) ggATAGAGCCGACCGTGCGCCAGCGGGCCGGCGGCAGCTCGCGCTT‑GGGCGGCTGCGGGGCATTGGGGCGGCCGGTCATGCGTCGCCACATGCGGATCAGCACATTGGTGCGCCATGGCTCGGGCACGACCTTGGTGCGTTTGATcggcg < 1:209873/150‑1 (MQ=255) gATAGAGCCGACCGTGCGCCAGCGGGCCGGCGGCAGCTCGCGCTT‑GGGCGGCTGCGGGGCATTGGGGCGGCCGGTCATGCGTCGCCACATGCGGATCAGCACATTGGTGCGCCATGGCTCGGGCACGACCTTGGTGCGTTTGATcggcgg > 2:1006058/1‑150 (MQ=255) aTAGAGCCGACCGTGCGCCAGCGGGCCGGCGGCAGCTCGCGCTT‑GGGCGGCTGCGGGGCATTGGGGCGGCCGGTCATGCGTCGCCACATGCGGATCAGCACATTGGTGCGCCATGGCTCGGGCACGACCTTGGTGCGTTTGATCGGCggg > 1:902328/1‑150 (MQ=255) tAGAGCCGACCGTGCGCCAGCGGGCCGGCGGCAGCTCGCGCTT‑GGGCGGCTGCGGGGCATTGGGGCGGCCGGTCATGCGTCGCCACATGCGGATCAGCACATTGGTGCGCCATGGCTCGGGCACGACCTTGGTGCGTTTGATCGGCgggg < 1:963974/150‑1 (MQ=255) agagCCGACCGTGCGCCAGCGGGCCGGCGGCAGCTCGCGCTT‑GGGCGGCTGCGGGGCATTGGGGCGGCCGGTCATGCGTCGCCACATGCGGATCAGCACATTGGTGCGCCATGGCTCGGGCACGACCTTGGTGCGTTTGATCGGCGGGGc < 2:322196/150‑1 (MQ=255) ccGNCCGTGCGCCAGCGGGCCGGCGGCAGCTCGCGCTT‑GGGCGGCTGCGGGGCATTGGGGCGGCCGGTCATGCGTCGCCACATGCGGATCAGCACATTGGTTCGCCATGGCTCGGGCACGACCTTAGTGCGTTTGATCGGCGGGGCGATc > 2:694865/1‑150 (MQ=255) ccGACCGTGCGCCAGCGGGCCGGCGGCAGCTCGCGCTT‑GGGCGGCTGCGGGGCATTGGGGCGGCCGGTCATGCGTCGCCACATGCGGATCAGCACATTGGTGCGCCATGGCTCGGGCACGACCTTGGTGCGTTTGATCGGCGGGGCGATc < 2:149311/150‑1 (MQ=255) ccGACCGTGCGCCAGCGGGCCGGCGGCAGCTCGCGCTT‑GGGCGGCTGCGGGGCATTGGGGCGGCCGGTCATGCGTCGCCACATGCGGATCAGCACATTGGTGCGCCATGGCTCGGGCACGACCTTGGTGCGTTTGATCGGCGGGGCGATc > 1:1071862/1‑150 (MQ=255) cGTGCGCCAGCGGGCCGGCGGCAGCTCGCGCTT‑GGGCGGCTGCGGGGCATTGGGGCGGCCGGTCATGCGTCGCCACATGCGGATCAGCACATTGGTGCGCCATGGCTCGGGCACGACCTTGGTGCGTTTGATCGGCGGGGCGATCTTCAg < 2:1071862/150‑1 (MQ=255) ccAGCGGGCCGGCGGCAGCTCGCGCTT‑GGGCGGCTGCGGGGCATTGGGGCGGCCGGTCATGCGTCGCCACATGCGGATCAGCACATTGGTGCGCCATGGCTCGGGCACGACCTTGGTGCGTTTGATCGGCGGGGCGATCTTCAGGCACAg < 1:1006058/150‑1 (MQ=255) cAGCGGGCCGGCGGCAGCTCGCGCTT‑GGGCGGCTGCGGGGCATTGGGGCGGCCGGTCATGCGTCGCCACATGCGGATCAGCACATTGGTGCGCCATGGCTCGGGCACGACCTTGGTGCGTTTGATCGGCGGGGCGATCTTCAGGCACAgg < 1:923370/150‑1 (MQ=255) ccgctggcggcAGCTCGCGCTT‑GGGCGGCTGCGGGGCATTGGGGCGGCCGGTCATGCGTCGCCACATGCGGATCAGCACATTGGTGCGCCATGGCTCGGGCACGACCTTGGTGCGTTTGATCGGCGGGGCGATCTTCAGGCACAggcggc > 1:854971/6‑150 (MQ=255) ccGGCGGCAGCTCGCGCTT‑GGGCGGCTGCGGGGCATTGGGGCGGCCGGTCATGCGTCGCCACATGCGGATCAGCACATTGGTGCGCCATGGCTCGGGCACGACCTTGGTGCGTTTGATCGGCGGGGCGATCTTCAGGCACAGGCGGCCGc > 2:165145/1‑150 (MQ=255) cAGCTCGCGCTT‑GGGCGGCTGCGGGGCATTGGCGCGGCCTGTCAGGCGTCGCTACATGCGGATCAGCACATTGGTGCTCCATGGCTCGGGCACGACCTTGGTGCGTTTGATCGCCGGGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTc < 1:144805/150‑1 (MQ=255) gCTCGCGCTT‑GGGCGGCTGCGGGGCATTGGGGCGGCCGGTCATGCGTCGCCACATGCGGATCAGCACATTGGTGCGCCATGGCTCGGGCACGACCTTGGTGCGTTTGATCGGCGGGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGa < 2:172396/150‑1 (MQ=255) gcccgtcgggCGGCTGCGGGGCATTGGGGCGGCCGGTCATGCGTCGCCACATGCGGATCAGCACATTGGTGCGCCATGGCTCGGGCACGACCTTGGTGCGTTTGATCGGCGGGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGc < 2:907180/143‑1 (MQ=255) cgcTT‑GGGCGGCTGCGGGGCATTGGGGCGGCCGGTCATGCGTCGCCACATGCGGAt < 2:1064645/56‑1 (MQ=255) cgcTT‑GGGCGGCTGCGGGGCATTGGGGCGGCCGGTCATGCGTCGCCACATGCGGAt > 1:1064645/1‑56 (MQ=255) gcTT‑GGGCGGCTGCGGGGCATTGGGGCGGCCGGTCATGCGTCGCCACATGCGGATCAGCACATTGGTGCGCCATGGCTCGGGCACGACCTTGGTGCGTTTGATCGGCGGGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGa < 2:217133/150‑1 (MQ=255) | ACCAGCCCTGGTACGGCAGGATGCCTTTCATGTACCAGCCAGCGACGATGGTCTGGCCGATCATCAGCGCCAGCAGAATGTAACGGCGGATAGAGCCGACCGTGCGCCAGCGGGCCGGCGGCAGCTCGCGCTT‑GGGCGGCTGCGGGGCATTGGGGCGGCCGGTCATGCGTCGCCACATGCGGATCAGCACATTGGTGCGCCATGGCTCGGGCACGACCTTGGTGCGTTTGATCGGCGGGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGA > NC_002947/5726520‑5726798 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |