| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,793,308 | G→A | 9.5% | R27C (CGC→TGC) | gltD ← | glutamate synthase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,793,308 | 0 | G | A | 9.5% | 108.5 / 2.2 | 42 | R27C (CGC→TGC) | gltD | glutamate synthase subunit beta |
| Reads supporting (aligned to +/- strand): ref base G (15/23); new base A (2/2); total (17/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01 | |||||||||||
CCAGCTTCAACCAGTTGGGGATGAAGTTGTGTACCGGGCACTTCCACTCGCAATACGGGTTACCGCAGCCCAGGCAGCGGTGCGCCTGCTCCACGGACTGCTGCGGCTTGAACGGTTCGTAGATTTCCACGAACTCCTTCTTGCGCTGGCGCAGCAGCTTTTTTTTCGGGTCCTTTCGGCCCACTTCGATGAACTGGAAGTCGTTGTTCAGACGTTCAGCCATTTTTCAAAACCTCTTTACAGCAGCTGCAAGCTACACGCTGCAAGCCGCAAGACGATCACTTAAGCCGG > NC_002947/5793164‑5793454 | ccAGCTTCAACCAGTTGGGGATGAAGTTGTGTACCGGGCACTTCCACTCGCAATACGGGTTACCGCAGCCCAGGCAGCGGTGCGCCTGCTCCACGGACTGCTGCGGCTTGAACGGTTCGTAGATTTCCACGAACTCCTTCTTGCGCTGgc < 2:1046747/150‑1 (MQ=255) gCTTCAACCAGTTGGGGATGAAGTTGTGTACCGGGCACTTCCACTCGCAATACGGGTTACCGCAGCCCAGGCAGCGGTGCGCCTGCTCCACGGACTGCTGCGGCTTGAACGGTTCGTAGATTTCCACGAACTCCTTCTTGCGCTGGCgca < 2:49788/150‑1 (MQ=255) aCCAGTTGGGGATGAAGTTGTGTACCGGGCACTTCCACTCGCAATACGGGTTACCGCAGCCCAGGCAGCGGTGCGCCTGCTCCACGGACTGCTGCGGCTTGAACGGTTCGTAGATTTCCACGAACTCCTTCTTGCGCTGGCGCAGCAGCt > 2:168744/1‑150 (MQ=255) aCCAGTTGGGGATGAAGTTGTGTACCGGGCACTTCCACTCGCAATACGGGTTACCGCAGCCCAGGCAGCGGTGCGCCTGCTCCACGGACTGCTGCGGCTTGAACGGTTCGTAGATTTCCACGAACTCCTTCTTGCGCTGGCGCAGCAGCt > 1:677706/1‑150 (MQ=255) ttGGGGATGAAGTTGTGTACCGGGCACTTCCACTCGCAATACGGGTTACCGCAGCCCAGGCAGCGGTGCGCCTGCTCCACGGACTGCTGCGGCTTGAACGGTTCGTAGATTTCCACGAACTCCTTCTTGCGCTGGCGCAGCAGCtttttt > 1:253963/1‑150 (MQ=255) gggATGAAGTTGTGTACCGGGCACTTCCACTCGCAATACGGGTTACCGCAGCCCAGGCAGCGGTGCGCCTGCTCCACGGACTGCTGCGGCTTGAACGGTTCGTAGATTTCCACGAACTCCTTCTTGCGCTGGCGCAGCAGCTTTTTTTTc < 2:253963/150‑1 (MQ=255) aTGAAGTTGTGTACCGGGCACTTCCACTCGCAATACGGGTTACCGCAGCCCAGGCAGCGGTGCGCCTGCTCCACGGACTGCTGCGGCTTGAACGGTTCGTAGATTTCCACGAACTCCTTCTTGCGCTGGCGCAGCAGCTTTTTTTTCggg < 2:974258/150‑1 (MQ=255) ttGTGTACCGGGCACTTCCACTCGCAATACGGGTTACCGCAGCCCAGGCAGCGGTGCGCCTGCTCCACGGACTGCTGCGGCTTGAACGGTTCGTAGATTTCCACGAACTCCTTCTTGCGCTGGCGCAGCAGCTTTTTTTTCGGGTCCttt < 1:567786/150‑1 (MQ=255) ccGGGCACTTCCACTCGCAATACGGGTTACCGCAGCCCAGGCAGCGGTGCGCCTGCTCCACGGACTGCTGCGGCTTGAACGGTTCGTAGATTTCCACGAACTCCTTCTTGCGCTGGCGCAGCAGCTTTTTTTTCGGGTCCTTTCGGCCCa > 1:169218/1‑150 (MQ=255) cGGGCACTTCCACTCGCAATACGGGTTACCGCAGCCCAGGCAGCGGTGCGCCTGCTCCACGGACTGCTGCGGCTTGAACGGTTCGTAGATTTCCACGAACTCCTTCTTGCGCTGGCGCAGCAGCTTTTTTTTCGGGTCCTTTCGGc > 2:700533/1‑146 (MQ=255) cGGGCACTTCCACTCGCAATACGGGTTACCGCAGCCCAGGCAGCGGTGCGCCTGCTCCACGGACTGCTGCGGCTTGAACGGTTCGTAGATTTCCACGAACTCCTTCTTGCGCTGGCGCAGCAGCTTTTTTTTCGGGTCCTTTCGGc < 1:700533/146‑1 (MQ=255) cTTCCACTCGCAATACGGGTTACCGCAGCCCAGGCAGCGGTGCGCCTGCTCCACGGACTGCTGCGGCTTGAACGGTTCGTAGATTTCCACGAACTCCTTCTTGCGCTGGCGCAGCAGCTTTTTTTTCGGGTCCTTTCGGCCCACTTCGAt < 2:991234/150‑1 (MQ=255) ccACTCGCAATACGGGTTACCGCAGCCCAGGCAGCGGTGCGCCTGCTCCACGGACTGCTGCGGCTTGAACGGTTCGTAGATTTCCACGCACCCGTTGCGCCACTGTCGCAGCAGCTTTTTTTTCTGGTCCTTTCGGCCCACTTCGATGaa > 2:30295/1‑150 (MQ=255) cACTCGCAATACGGGTTACCGCAGCCCAGGCAGCGGTGCGCCTGCTCCCCGGACTGCTGCGGCTTGAACGGTTCGTAGATTTCCACGAACTCCTTCTTGCGCTGGCGCAGCAGCTTTTTTTTCGGGTCCTTTCGGCCCACTTCGATGAAc < 1:493085/150‑1 (MQ=255) aTACGGGTTACCGCAGCCCAGGCAGCGGTGCGCCTGCTCCACGGACTGCTGCGGCTTGAACGGTTCGTAGATTTCCACGAACTCCTTCTTGCGCTGGCGCAGCAGCTTTTTTTTCGGGTCCTTTCGGCCCACTTCGATGAACTGGAAGTc > 1:326806/1‑150 (MQ=255) tACCGCAGCCCAGGCAGCGGTGCGCCTGCTCCACGGACTGCTGCGGCTTGAACGGTTCGTAGATTTCCACGAACTCCTTCTTGCGCTGGCGCAGCAGCTTTTTTTTCGGGTCCTTTCGGCCCACTTCGATGAACTGGAAGTCGTTGTTCa < 2:838722/150‑1 (MQ=255) cGCAGCCCAGGCAGCGGTGCGCCTGCTCCACGGACTGCTGCGGCTTGAACGGTTCGTAGATTTCCACGAACTCCTTCTTGCGCTGGCGCAGCAGCTTTTTTTTCGGGTCCTTTCGGCCCACTTCGATGAACTGGAAGTCGTTGTTCAGAc > 1:642182/1‑150 (MQ=255) cGCAGCCCAGGCAGCGGTGCGCCTGCTCCACGGACTGCTGCGGCTTGAACGGTTCGTAGATTTCCACGAACTCCTTCTTGCGCTGGCGCAGCAGCTTTTTTTTCGGGTCCTTTCGGCCCACTTCGATGAACTGGAAGTCGTTGTTCAGAc < 2:923360/150‑1 (MQ=255) cGCAGCCCAGGCAGCGGTGCGCCTGCTCCACGGACTGCTGCGGCTTGAACGGTTCGTAGATTTCCACGAACTCCTTCTTGCGCTGGCGCAGCAGCTTTTTTTTCGGGTCCTTTCGGCCCACTTCGATGAACTGGAAGTCGTTGTTCAGAc < 2:1069669/150‑1 (MQ=255) cccAGGCAGCGGTGCGCCTGCTCCACGGACTGCTGCGGCTTGAACGGTTCGTAGATTTCCACGAACTCCTTCTTGCACTGGCGCAGCAGCTTTTTTTTCGGGTCCTTTCGGCCCACTTCGATGAACTGGAAGTCGTTGTTCAGACGTTCa < 2:383293/150‑1 (MQ=255) tGCGCCTGCTCCACGGACTGCTGCGGCTTGAACGGTTCGTAGATTTCCACGAACTCCTTCTTGCGCTGGCGCAGCAGCTTTTTTTTCGGGTCCTTTCGGCCCACTTCGATGAACTGGAAGTCGTTGTTCAGACGTTCAGCCATTTTTc > 2:387718/1‑148 (MQ=255) tGCGCCTGCTCCACGGACTGCTGCGGCTTGAACGGTTCGTAGATTTCCACGAACTCCTTCTTGCGCTGGCGCAGCAGCTTTTTTTTCGGGTCCTTTCGGCCCACTTCGATGAACTGGAAGTCGTTGTTCAGACGTTCAGCCATTTTTc < 1:387718/148‑1 (MQ=255) cTCCACGGACTGCTGCGGCTTGAACGGTTCGTAGATTTCCACGAACTCCTTCTTGCGCTGGCGCAGCAGCTTTTTTTTCGGGTCCTTTCGGCCCACTTCGATGAACTGGAAGTCGTTGTTCAGACGTTCAGCCATTTTTCAAAACCTCtt < 1:493226/150‑1 (MQ=255) aCGGACTGCTGCGGCTTGAACGGTTCGTAGATTTCCACGAACTCCTTCTTGCGCTGGCGCAGCAGCTTTTTTTTCGGGTCCTTTCGGCCCACTTCGATGAACTGGAAGTCGTTGTTCAGACCTTCAGCCATTTTTCAAAACCTCTTTAca < 2:482532/150‑1 (MQ=255) tgcngcGGCTTGAACGGTTCGTAGATTTCCACGAACTCCTTCTTGCGCTGGCGCAGCAGCTTTTTTTTCGGGTCCTTTCGGCCCACTTCGATGAACTGGAAGTCGTTGTTCAGACGTTCAGCCATTTTTCAAAACCTCTTTACAGCAGCt > 2:697554/1‑150 (MQ=255) gTAGATTTCCACGAACTCCTTCTTGCGCTGGCGCAGCAGCTTTTTTTTCGGGTTTTTTCGGCCCACTTCGATGAACTGGAAGTCGTTGTTCAGACGTTCAGCCA‑TTTTCAAAACCTCTTTACAGCAGCTGCAAGCTACACGCTGCAAGcc > 2:927290/1‑150 (MQ=255) tAGATTTCCACGAACTCCTTCTTGCGCTGGCGCAGCAGCTTTTTTTTCGGGTCCTTTCGGCCCACTTCGATGAACTGGAAGTCGTTGTTc > 2:232106/1‑90 (MQ=255) tAGATTTCCACGAACTCCTTCTTGCGCTGGCGCAGCAGCTTTTTTTTCGGGTCCTTTCGGCCCACTTCGATGAACTGGAAGTCGTTGTTc < 1:114455/90‑1 (MQ=255) tAGATTTCCACGAACTCCTTCTTGCGCTGGCGCAGCAGCTTTTTTTTCGGGTCCTTTCGGCCCACTTCGATGAACTGGAAGTCGTTGTTc > 2:114455/1‑90 (MQ=255) tAGATTTCCACGAACTCCTTCTTGCGCTGGCGCAGCAGCTTTTTTTTCGGGTCCTTTCGGCCCACTTCGATGAACTGGAAGTCGTTGTTc < 1:232106/90‑1 (MQ=255) tAGATTTCCACGAACTCCTTCTTGCGCTGGCGCAGCAGCTTTTTTTTCGGGTCCTTTCGGCCCACTTCGATGAACTGGAAGTCGTTGTTCAGACGTTCAGCCATTTTTCAAAACCTCTTTACAGCAGCTGCAAGCTACACGCTGCAAGcc < 2:326806/150‑1 (MQ=255) tAGATTTCCACGAACTCCTTCTTGCGCTGGCGCAGCAGCTTTTTTTTCGGGTCCTTTCGGCCCACTTCGATGAACTGGAAGTCGTTGTTCAGACGTTCAGCCATTTTTCAAAACCTCTTTACAGCAGCTGCAAGCTACACGCTGCAAGcc > 2:82696/1‑150 (MQ=255) aGATTTCCACGAACTCCTTCTTGCGCTGGCGCAGCAGCTTTTTTTTCGGGTCCTTTCGGCCCACTTCGATGAACTGGAAGTCGTTGTTCAGACGTTCAGCCATTTTTCAAAACCTCTTTACAGCAGCTGCAAGCTACACGCTGCAAGCCg < 2:642182/150‑1 (MQ=255) gATTTCCACGAACTCCTTCTTGCGCTGGCGCAGCAGCTTTTTTTTCGGGTCCTTTCGGCCCACTTCGATGAACTGGAAGTCGTTGTTCAGACGTTCAGCCATTTTTCAAAACCTCTTTACAGCAGCTGCAAGCTACACGCTGCAAGCCGc < 2:169218/150‑1 (MQ=255) tttCCACGAACTCCTTCTTGCGCTGGCGCAGCAGCTTTTTTTTCGGGTCCTTTCGGCCCACTTCGATGAACTGGAAGTCGTTGTTCAGACGTTCAGCCATTTTTCAAAACCTCTTTACAGCAGCTGCAAGCTACACGCTGCAAGCCGCaa < 2:975244/150‑1 (MQ=255) cGAACTCCTTCTTGCGCTGGCGCAGCAGCTTTTTTTTCGGGTCCTTTCGGCCCACTTCGATGAACTGGAAGTCGTTGTTCAGACGTTCAGCCATTTTTCAAAACCTCTTTACAGCAGCTGCAAGCTACACGCTGCAAGCCGCAAGACGAt < 1:82696/150‑1 (MQ=255) tCCTTCTTGCGCTGGCGCAGCAGCTTTTTTTTCGGGTCCTTTCGGCCCACTTCGATGAACTGGAAGTCGTTGTTCAGACGTTCAGCCATTTTTCAAAACCTCTTTa > 2:115219/1‑106 (MQ=255) tCCTTCTTGCGCTGGCGCAGCAGCTTTTTTTTCGGGTCCTTTCGGCCCACTTCGATGAACTGGAAGTCGTTGTTCAGACGTTCAGCCATTTTTCAAAACCTCTTTa < 1:115219/106‑1 (MQ=255) cttcttGCGCTGGCGCAGCAGCTTTTTTTTTGTTTCCTTTCGGCCCACTTCGGTTAACTGGAAGTCGTTGTTCAGACGTTCAGCCATTTTTCAAAACCTCTTTACAGCAGCTGCAAGCTACACGCTGCAAGCCGCAAGACGATCACTTaa > 2:937426/1‑150 (MQ=255) cttcttGCACTGGCGCAGCAGCTTTTTTTTCGGGTCCTTTCGGCCCACTTCGATGAACTGGAAGTCGTTGTTCAGACGTTCAGCCATTTTTCaaaa > 2:76327/1‑96 (MQ=255) cttcttGCACTGGCGCAGCAGCTTTTTTTTCGGGTCCTTTCGGCCCACTTCGATGAACTGGAAGTCGTTGTTCAGACGTTCAGCCATTTTTCaaaa < 1:76327/96‑1 (MQ=255) tGCGCTGGCGCAGCAGCTTTTTTTTCGGGTCCTTTCGGCCCACTTCGATGAACTGGAAGTCGTTGTTCAGACGTTCAGCCATTTTTCAAAACCTCTTTACAGCAGCTGCAAGCTACACGCTGCAAGCCGCAAGACGATCACTTAAGCCgg < 2:677706/150‑1 (MQ=255) | CCAGCTTCAACCAGTTGGGGATGAAGTTGTGTACCGGGCACTTCCACTCGCAATACGGGTTACCGCAGCCCAGGCAGCGGTGCGCCTGCTCCACGGACTGCTGCGGCTTGAACGGTTCGTAGATTTCCACGAACTCCTTCTTGCGCTGGCGCAGCAGCTTTTTTTTCGGGTCCTTTCGGCCCACTTCGATGAACTGGAAGTCGTTGTTCAGACGTTCAGCCATTTTTCAAAACCTCTTTACAGCAGCTGCAAGCTACACGCTGCAAGCCGCAAGACGATCACTTAAGCCGG > NC_002947/5793164‑5793454 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |