Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,846,239 T→C 15.5% T217A (ACC→GCC)  mutM ← formamidopyrimidine‑DNA glycosylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,846,2390TC15.5% 59.0 / 4.5 33T217A (ACC→GCC) mutMformamidopyrimidine‑DNA glycosylase
Reads supporting (aligned to +/- strand):  ref base T (16/11);  new base C (3/2);  total (20/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.11e-01

GGGCAGTACACGCTCGCCCTCTGCCCCAGCTTCACTTCGCGCAACTCAGTGCCGCATACCTTGCACGGCTGCCCGCCACGCCCGTACACAAACAGTTCCTGCTGGAAGTACCCCGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGTGGTACCGCCCTGCTCGATGGCCGCCGCCAGCACCCGTTTGATCTCGATCGCCAGTTTCAGGTAGCGCGCCCGGGAAATCCCGCCCGCCTCTCGGCGTGGGTCGATACCGGCGGCAAACAACGCCTCGGTGGCGTAGATGTTGCCC  >  NC_002947/5846091‑5846384
                                                                                                                                                    |                                                                                                                                                 
gggCAGTACACGCTCGCCCTCTGCCCCAGCTTCACTTCGCGCAACTCAGTGCCGCATACCTTGCACGGCTGCCCGCCACGCCCGTACACAAACAGTTCCTGCTGGAAGTACCCCGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGggtg                                                                                                                                                  <  2:79724/150‑1 (MQ=255)
      tACACGCTCGCACTCTGCCCCAGCTTCACTTCGCGCAACTCAGTGCCGCATACCTTGCACGGCTGCCCGCCACGCCCGTACACAAACAGTTCCTGCTGGAAGTACCCCGGCTGCCCGTCGCCACCGCTAAAGTCGGGCAGGGTGGTACCg                                                                                                                                            >  1:277869/1‑150 (MQ=255)
           gCTCGCCCTCTGCCCCAGCTTCACTTCGCGCAACTCAGTGCCGCATACCTTGCACGGCTGCCCGCCACGCCCGTACACAACAAGTTCCTGCTGGAAGTACCCCGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGTGGTACCGCCCTg                                                                                                                                       >  2:875368/1‑150 (MQ=255)
             tCGCCCTCTGCCCCAGCTTCACTTCGCGCAACTCAGTGCCGCATACCTTGCACGGCTGCCCGCCACGCCCGTACACAAACAGTTCCTGCTGGAAGTACCCCGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGTGGTACCGCCCTGcc                                                                                                                                     >  2:377358/1‑149 (MQ=255)
                 ccTCTGCCCCAGCTTCACTTCGCGCAACTCAGTGCCGCATACCTTGCACGGCTGCCCGCCACGCCCGTACACAAACAGTTCCTGCTGGAAGTACCCCGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGTGGTACCGCCCTGCTCGAt                                                                                                                                 <  2:979433/150‑1 (MQ=255)
                     tGCCCCAGCTTCACTTCGCGCAACTCAGTGCCGCATACCTTGCACGGCTGCCCGCCACGCCCGTACACAAACAGTTCCTGCTGGAAGTACCCCGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGTGGTACCGCCCTGCTCGATGgcc                                                                                                                             <  2:754776/150‑1 (MQ=255)
                         ccAGCTTCACTTCGCGCAACTCAGTGCCGCATACCTTGCACGGCTGCCCGCCACGCCCGTACACAAACAGTTCCTGCTGGAAGTACCCCGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGTGGTACCGCCCTGCTCGATGgccgccg                                                                                                                         >  1:547402/1‑150 (MQ=255)
                                  cTTCGCGCAACTCAGTGCCGCATACCTTGCACGGCTGCCCGCCACGCCCGTACACAAACAGTTCCTGCTGGAAGTACCCCGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGCGGTACCGCCCTGCCCGATGGCCGCCGACAGCAccc                                                                                                                >  2:149986/1‑150 (MQ=255)
                                   ttCGCGCAACTCAGTGCCGCATACCTTGCCCGGCTGCCCGCCACGCCCGTACACAAACAGTTCCTGCTGGAAGTACCCCGGCTGCCCGTCGCCACCGATAAAGGCGCGCAGGGAGGTACCGCCCTGCCCGCTGGCCGCCGGCCGCAgccc                                                                                                               >  2:333029/1‑149 (MQ=255)
                                   ttCGCGCAACTCAGTGCCGCATACCTTGCACGGCTGCCCGCCACGCCCGTACACAAACAGTTCCTGCTGGAAGTACCCCGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGCGGTACCACCCTGc                                                                                                                                      >  1:528753/1‑127 (MQ=255)
                                   ttCGCGCAACTCAGTGCCGCATACCTTGCACGGCTGCCCGCCACGCCCGTACACAAACAGTTCCTGCTGGAAGTACCCCGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGCGGTACCACCCTGc                                                                                                                                      <  2:528753/127‑1 (MQ=255)
                                     cgcgCAACTCAGTGCCGCATACCTTGCACGGCTGCCCGCCACGCCCGTACACAAACAGTTCCTGCTGGAAGTACCCCGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGTGGTACCGCCCTGCTCGATGGCCGCCGCCAGCACCCGtt                                                                                                             <  2:826964/150‑1 (MQ=255)
                                                          ccTTGCACGGCTGCCCGCCACGCCCGTACACAAACAGTTCCTGCTGGAAGTACCCCGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGTGGTACCGCCCTGCTCGATGGCCGCCGCCAGCACCCGTTTGATCTCGATCGCCa                                                                                              <  1:80589/144‑1 (MQ=255)
                                                          ccTTGCACGGCTGCCCGCCACGCCCGTACACAAACAGTTCCTGCTGGAAGTACCCCGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGTGGTACCGCCCTGCTCGATGGCCGCCGCCAGCACCCGTTTGATCTCGATCGCCa                                                                                              >  2:80589/1‑144 (MQ=255)
                                                          ccTTGCACGGCTGCCCGCCACGCCCGTACACAAACAGTTCCTGCTGGAAGTACCCAGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGTGGTACCGCCCTGCTCGATGGCCGCCGCCAGCACCCGTTTGATCTCGATCGCCAGTTTCa                                                                                        <  1:853040/150‑1 (MQ=255)
                                                               cACGGCTGCCCGCCACGCCCGTACACAAACAGTTCCTGCTGGAAGTACCCCGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGTGGTACCGCCCTGCTCGATGGCCGCCGCCAGCACCCGTTTGATCTCGATCGCCAGTTTCAGGTAg                                                                                   >  2:601480/1‑150 (MQ=255)
                                                                    gggCCCGCCACGCCCGTACACAAACAGTTCCTGCTGGAAGTACCCCGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGTGGTACCGCCCTGCTCGATGGCCGCCGCCAGCACCCGTTTGATCTCGATCGCCAGTTTCAGGTAgcgcgc                                                                              <  2:304518/148‑1 (MQ=255)
                                                                      gCCCGCCACGCCCGTACACAAACAGTTCGTGCTGGAGGTACCCCGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGTGGTACCGCCCTGCTCGATGGCCGCCGCCAGCACCCGTTTGATCTCGATCGCCAGTTTCAGGTAGCGCGccc                                                                            >  1:753383/1‑150 (MQ=255)
                                                                        ccGCCACGCCCGTACACAAACAGTTCCTGCTGGAAGTACCCCGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGTGGTACCGCCCTGCTCGATGGCCGCCGCCAGCACCCGTTTGATCTCGATCGCCAGTTTCAGGTAGCGCGCCCgg                                                                          <  2:547402/150‑1 (MQ=255)
                                                                           ccACGCCCGTACACAAACAGTTCCTGCTGGAAGTACCCCGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGTGGTACCGCCCTGCTCGATGGCCGCCGCCAGCACCCGtt                                                                                                             >  1:910225/1‑112 (MQ=255)
                                                                           ccACGCCCGTACACAAACAGTTCCTGCTGGAAGTACCCCGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGTGGTACCGCCCTGCTCGATGGCCGCCGCCAGCACCCGtt                                                                                                             <  2:910225/112‑1 (MQ=255)
                                                                                  cGTACACACACCGTCCCTGCTGGAAGTACCCCGGCTGCCCGTCGCCACCGAGCAAGCCGCTCAGGGTGGTCCCGCCCTGCTCGATGGCCGCCGCCAGCACCCGTTTGATCTCGATCGCCAGTTTCAGGTAGCGCGCCCGGGAAATcccgc                                                                <  2:277869/150‑1 (MQ=255)
                                                                                       acaAACAGTTCCTGCTGGAAGTACCCCGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGTGGTACCGCCCTGCTCGATGGCCGCCGCCAGCACCCGTTTGATCTCGATCGCCAGTTTCAGGTAGCGCGCCCGGGAAATCCcgcccgcc                                                           >  1:768063/1‑150 (MQ=255)
                                                                                                tCCTGCTGGAAGTACCCCGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGTGGTACCGCCCTGCTCGATGGCCGCCGCCCGCACCCGTTTGATCTCGATCGCCAGTTTCAGGTAGCGCGCCCGGGAAATCCCGCCCGCCTCTCGGCGt                                                  <  1:875368/150‑1 (MQ=255)
                                                                                                   tgctgGAAGTACCCCGGCTGCCCGTCGCCACCGATAAAGTCGCTCAGGGTGGTACCGCCCTGCTCGATGGCCGCCGCCAGCACCCGTTTGATCTCGATCGCTAGTTTCAGGTAGCGAGCCCGGGAAATCCCGGCCGCCTCTCGGCGTggg                                               >  2:293510/1‑150 (MQ=255)
                                                                                                              ccccGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGTGGTACCGCCCTGCTCGATGGCCGCCGCCAGCACCCGTTTGATCTCGATCGCCAGTTTCAGGTAGCGCGCCCGGGAAATCCCGCCCGCCTCTCGGCGTGGGTCGATACcggc                                    >  2:380508/1‑150 (MQ=255)
                                                                                                                ccGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGTGGTACCGCCCTGCTCGATGGCCGCCGCCAGCACCCGTTTGATCTCGATCGCCAGTTTCAGGTAGCGCGCCCGGGAAATCCCGCCCGCCTCTCGGCGTGGGTGGATACcggccg                                  >  2:424234/1‑148 (MQ=255)
                                                                                                                       cccGTCGCCACCGATAAAGTCGCGCAGGGTGGTACCGCCCTGCTCGATGGCCGCCGCCAGCACCCGTTTGATCGCCAGCGCCAGTTTCAGGTAGCGCGCCCCGGAAAGCCCGCCCGCGTCTCGGCGTCGGCCGATACCGGCGGGAaacaa                           >  2:917333/1‑150 (MQ=255)
                                                                                                                        ccGTCGCCACCGATAAAGTCGCGCAGGGTGGTACCGCCCTGCTCGATGGCCGCCGCCAGCACCCGTTTGATCTCGATCGCCAGTTTCAGGTAGCGCGCCCGGGAAATCCCGCCCGCCTCTCGGCGTGGGTCGATACCGGCGGCAaacaac                          >  1:650318/1‑150 (MQ=255)
                                                                                                                               cACCGATAAAGTCGCGCAGGGTGGGACCGCCCTGCTCGATGGCCGCCGCCAGCACCCGTTTGATCTCGATCGCCAGTTTCAGGTAGCGCGCCCGGGAAATCCCGCCCGCCTCTCGGCGTGGGTCGATACCGGCGGCAAACAACGCCTCgg                   >  2:943348/1‑150 (MQ=255)
                                                                                                                                            gcgcAGGGTGGTACCGCCCTGCTCGATGGCCGCCGCCAGCACCCGTTTGATCTCGATCGCCAGTTTCAGGTAGCGCGCCCGGGAAATCCCGCCCGCCTCTCGGCGTGGGTCGATACCGGCGGCAAACAACGCCTCGGTGGCGTAGATGtt      <  2:650318/150‑1 (MQ=255)
                                                                                                                                              gcAGGGCGGTACCACCCTGCGCGATGGCCGCCGCCAGCACCCGTTTGATCTCGATCGCCAGTTTCAGGTAGCGCGCCCGGGAAATCCCGCCCGCCTCTCGGCGTGGGTCGATACcggcgg                                  <  1:164002/120‑1 (MQ=255)
                                                                                                                                              gcAGGGCGGTACCACCCTGCGCGATGGCCGCCGCCAGCACCCGTTTGATCTCGATCGCCAGTTTCAGGTAGCGCGCCCGGGAAATCCCGCCCGCCTCTCGGCGTGGGTCGATACcggcgg                                  >  2:164002/1‑120 (MQ=255)
                                                                                                                                                aGGGTGGTACCGCCCTGCTCGATGGCCGCCGCCAGCACCCGTTTGATCTCGATCGCCAGTTTCAGGTAGCGCGCCCGGGAAATCCCGCCCGCCTCTCGGCGTGGGTCGATACCGGCGGCAAACAACGCCTCGGTGGCGTAGATGTTGccc  >  2:650101/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GGGCAGTACACGCTCGCCCTCTGCCCCAGCTTCACTTCGCGCAACTCAGTGCCGCATACCTTGCACGGCTGCCCGCCACGCCCGTACACAAACAGTTCCTGCTGGAAGTACCCCGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGTGGTACCGCCCTGCTCGATGGCCGCCGCCAGCACCCGTTTGATCTCGATCGCCAGTTTCAGGTAGCGCGCCCGGGAAATCCCGCCCGCCTCTCGGCGTGGGTCGATACCGGCGGCAAACAACGCCTCGGTGGCGTAGATGTTGCCC  >  NC_002947/5846091‑5846384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑