Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,846,246 G→A 12.5% G214G (GGC→GGT)  mutM ← formamidopyrimidine‑DNA glycosylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,846,2460GA12.5% 65.8 / 3.6 32G214G (GGC→GGT) mutMformamidopyrimidine‑DNA glycosylase
Reads supporting (aligned to +/- strand):  ref base G (17/11);  new base A (2/2);  total (19/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.76e-01

TACACGCTCGCCCTCTGCCCCAGCTTCACTTCGCGCAACTCAGTGCCGCATACCTTGCACGGCTGCCCGCCACGCCCGTACACAAACAGTTCCTGCTGGAAGTACCCCGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGTGGTACCGCCCTGCTCGATGGCCGCCGCCAGCACCCGTTTGATCTCGATCGCCAGTTTCAGGTAGCGCGCCCGGGAAATCCCGCCCGCCTCTCGGCGTGGGTCGATACCGGCGGCAAACAACGCCTCGGTGGCGTAGATGTTGCCC  >  NC_002947/5846097‑5846384
                                                                                                                                                     |                                                                                                                                          
tACACGCTCGCACTCTGCCCCAGCTTCACTTCGCGCAACTCAGTGCCGCATACCTTGCACGGCTGCCCGCCACGCCCGTACACAAACAGTTCCTGCTGGAAGTACCCCGGCTGCCCGTCGCCACCGCTAAAGTCGGGCAGGGTGGTACCg                                                                                                                                            >  1:277869/1‑150 (MQ=255)
     gCTCGCCCTCTGCCCCAGCTTCACTTCGCGCAACTCAGTGCCGCATACCTTGCACGGCTGCCCGCCACGCCCGTACACAACAAGTTCCTGCTGGAAGTACCCCGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGTGGTACCGCCCTg                                                                                                                                       >  2:875368/1‑150 (MQ=255)
       tCGCCCTCTGCCCCAGCTTCACTTCGCGCAACTCAGTGCCGCATACCTTGCACGGCTGCCCGCCACGCCCGTACACAAACAGTTCCTGCTGGAAGTACCCCGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGTGGTACCGCCCTGcc                                                                                                                                     >  2:377358/1‑149 (MQ=255)
           ccTCTGCCCCAGCTTCACTTCGCGCAACTCAGTGCCGCATACCTTGCACGGCTGCCCGCCACGCCCGTACACAAACAGTTCCTGCTGGAAGTACCCCGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGTGGTACCGCCCTGCTCGAt                                                                                                                                 <  2:979433/150‑1 (MQ=255)
               tGCCCCAGCTTCACTTCGCGCAACTCAGTGCCGCATACCTTGCACGGCTGCCCGCCACGCCCGTACACAAACAGTTCCTGCTGGAAGTACCCCGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGTGGTACCGCCCTGCTCGATGgcc                                                                                                                             <  2:754776/150‑1 (MQ=255)
                   ccAGCTTCACTTCGCGCAACTCAGTGCCGCATACCTTGCACGGCTGCCCGCCACGCCCGTACACAAACAGTTCCTGCTGGAAGTACCCCGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGTGGTACCGCCCTGCTCGATGgccgccg                                                                                                                         >  1:547402/1‑150 (MQ=255)
                            cTTCGCGCAACTCAGTGCCGCATACCTTGCACGGCTGCCCGCCACGCCCGTACACAAACAGTTCCTGCTGGAAGTACCCCGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGCGGTACCGCCCTGCCCGATGGCCGCCGACAGCAccc                                                                                                                >  2:149986/1‑150 (MQ=255)
                             ttCGCGCAACTCAGTGCCGCATACCTTGCCCGGCTGCCCGCCACGCCCGTACACAAACAGTTCCTGCTGGAAGTACCCCGGCTGCCCGTCGCCACCGATAAAGGCGCGCAGGGAGGTACCGCCCTGCCCGCTGGCCGCCGGCCGCAgccc                                                                                                               >  2:333029/1‑149 (MQ=255)
                             ttCGCGCAACTCAGTGCCGCATACCTTGCACGGCTGCCCGCCACGCCCGTACACAAACAGTTCCTGCTGGAAGTACCCCGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGCGGTACCACCCTGc                                                                                                                                      <  2:528753/127‑1 (MQ=255)
                             ttCGCGCAACTCAGTGCCGCATACCTTGCACGGCTGCCCGCCACGCCCGTACACAAACAGTTCCTGCTGGAAGTACCCCGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGCGGTACCACCCTGc                                                                                                                                      >  1:528753/1‑127 (MQ=255)
                               cgcgCAACTCAGTGCCGCATACCTTGCACGGCTGCCCGCCACGCCCGTACACAAACAGTTCCTGCTGGAAGTACCCCGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGTGGTACCGCCCTGCTCGATGGCCGCCGCCAGCACCCGtt                                                                                                             <  2:826964/150‑1 (MQ=255)
                                                    ccTTGCACGGCTGCCCGCCACGCCCGTACACAAACAGTTCCTGCTGGAAGTACCCCGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGTGGTACCGCCCTGCTCGATGGCCGCCGCCAGCACCCGTTTGATCTCGATCGCCa                                                                                              >  2:80589/1‑144 (MQ=255)
                                                    ccTTGCACGGCTGCCCGCCACGCCCGTACACAAACAGTTCCTGCTGGAAGTACCCCGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGTGGTACCGCCCTGCTCGATGGCCGCCGCCAGCACCCGTTTGATCTCGATCGCCa                                                                                              <  1:80589/144‑1 (MQ=255)
                                                    ccTTGCACGGCTGCCCGCCACGCCCGTACACAAACAGTTCCTGCTGGAAGTACCCAGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGTGGTACCGCCCTGCTCGATGGCCGCCGCCAGCACCCGTTTGATCTCGATCGCCAGTTTCa                                                                                        <  1:853040/150‑1 (MQ=255)
                                                         cACGGCTGCCCGCCACGCCCGTACACAAACAGTTCCTGCTGGAAGTACCCCGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGTGGTACCGCCCTGCTCGATGGCCGCCGCCAGCACCCGTTTGATCTCGATCGCCAGTTTCAGGTAg                                                                                   >  2:601480/1‑150 (MQ=255)
                                                              gggCCCGCCACGCCCGTACACAAACAGTTCCTGCTGGAAGTACCCCGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGTGGTACCGCCCTGCTCGATGGCCGCCGCCAGCACCCGTTTGATCTCGATCGCCAGTTTCAGGTAgcgcgc                                                                              <  2:304518/148‑1 (MQ=255)
                                                                gCCCGCCACGCCCGTACACAAACAGTTCGTGCTGGAGGTACCCCGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGTGGTACCGCCCTGCTCGATGGCCGCCGCCAGCACCCGTTTGATCTCGATCGCCAGTTTCAGGTAGCGCGccc                                                                            >  1:753383/1‑150 (MQ=255)
                                                                  ccGCCACGCCCGTACACAAACAGTTCCTGCTGGAAGTACCCCGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGTGGTACCGCCCTGCTCGATGGCCGCCGCCAGCACCCGTTTGATCTCGATCGCCAGTTTCAGGTAGCGCGCCCgg                                                                          <  2:547402/150‑1 (MQ=255)
                                                                     ccACGCCCGTACACAAACAGTTCCTGCTGGAAGTACCCCGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGTGGTACCGCCCTGCTCGATGGCCGCCGCCAGCACCCGtt                                                                                                             <  2:910225/112‑1 (MQ=255)
                                                                     ccACGCCCGTACACAAACAGTTCCTGCTGGAAGTACCCCGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGTGGTACCGCCCTGCTCGATGGCCGCCGCCAGCACCCGtt                                                                                                             >  1:910225/1‑112 (MQ=255)
                                                                            cGTACACACACCGTCCCTGCTGGAAGTACCCCGGCTGCCCGTCGCCACCGAGCAAGCCGCTCAGGGTGGTCCCGCCCTGCTCGATGGCCGCCGCCAGCACCCGTTTGATCTCGATCGCCAGTTTCAGGTAGCGCGCCCGGGAAATcccgc                                                                <  2:277869/150‑1 (MQ=255)
                                                                                 acaAACAGTTCCTGCTGGAAGTACCCCGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGTGGTACCGCCCTGCTCGATGGCCGCCGCCAGCACCCGTTTGATCTCGATCGCCAGTTTCAGGTAGCGCGCCCGGGAAATCCcgcccgcc                                                           >  1:768063/1‑150 (MQ=255)
                                                                                          tCCTGCTGGAAGTACCCCGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGTGGTACCGCCCTGCTCGATGGCCGCCGCCCGCACCCGTTTGATCTCGATCGCCAGTTTCAGGTAGCGCGCCCGGGAAATCCCGCCCGCCTCTCGGCGt                                                  <  1:875368/150‑1 (MQ=255)
                                                                                             tgctgGAAGTACCCCGGCTGCCCGTCGCCACCGATAAAGTCGCTCAGGGTGGTACCGCCCTGCTCGATGGCCGCCGCCAGCACCCGTTTGATCTCGATCGCTAGTTTCAGGTAGCGAGCCCGGGAAATCCCGGCCGCCTCTCGGCGTggg                                               >  2:293510/1‑150 (MQ=255)
                                                                                                        ccccGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGTGGTACCGCCCTGCTCGATGGCCGCCGCCAGCACCCGTTTGATCTCGATCGCCAGTTTCAGGTAGCGCGCCCGGGAAATCCCGCCCGCCTCTCGGCGTGGGTCGATACcggc                                    >  2:380508/1‑150 (MQ=255)
                                                                                                          ccGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGTGGTACCGCCCTGCTCGATGGCCGCCGCCAGCACCCGTTTGATCTCGATCGCCAGTTTCAGGTAGCGCGCCCGGGAAATCCCGCCCGCCTCTCGGCGTGGGTGGATACcggccg                                  >  2:424234/1‑148 (MQ=255)
                                                                                                                 cccGTCGCCACCGATAAAGTCGCGCAGGGTGGTACCGCCCTGCTCGATGGCCGCCGCCAGCACCCGTTTGATCGCCAGCGCCAGTTTCAGGTAGCGCGCCCCGGAAAGCCCGCCCGCGTCTCGGCGTCGGCCGATACCGGCGGGAaacaa                           >  2:917333/1‑150 (MQ=255)
                                                                                                                  ccGTCGCCACCGATAAAGTCGCGCAGGGTGGTACCGCCCTGCTCGATGGCCGCCGCCAGCACCCGTTTGATCTCGATCGCCAGTTTCAGGTAGCGCGCCCGGGAAATCCCGCCCGCCTCTCGGCGTGGGTCGATACCGGCGGCAaacaac                          >  1:650318/1‑150 (MQ=255)
                                                                                                                         cACCGATAAAGTCGCGCAGGGTGGGACCGCCCTGCTCGATGGCCGCCGCCAGCACCCGTTTGATCTCGATCGCCAGTTTCAGGTAGCGCGCCCGGGAAATCCCGCCCGCCTCTCGGCGTGGGTCGATACCGGCGGCAAACAACGCCTCgg                   >  2:943348/1‑150 (MQ=255)
                                                                                                                                      gcgcAGGGTGGTACCGCCCTGCTCGATGGCCGCCGCCAGCACCCGTTTGATCTCGATCGCCAGTTTCAGGTAGCGCGCCCGGGAAATCCCGCCCGCCTCTCGGCGTGGGTCGATACCGGCGGCAAACAACGCCTCGGTGGCGTAGATGtt      <  2:650318/150‑1 (MQ=255)
                                                                                                                                        gcAGGGCGGTACCACCCTGCGCGATGGCCGCCGCCAGCACCCGTTTGATCTCGATCGCCAGTTTCAGGTAGCGCGCCCGGGAAATCCCGCCCGCCTCTCGGCGTGGGTCGATACcggcgg                                  >  2:164002/1‑120 (MQ=255)
                                                                                                                                        gcAGGGCGGTACCACCCTGCGCGATGGCCGCCGCCAGCACCCGTTTGATCTCGATCGCCAGTTTCAGGTAGCGCGCCCGGGAAATCCCGCCCGCCTCTCGGCGTGGGTCGATACcggcgg                                  <  1:164002/120‑1 (MQ=255)
                                                                                                                                          aGGGTGGTACCGCCCTGCTCGATGGCCGCCGCCAGCACCCGTTTGATCTCGATCGCCAGTTTCAGGTAGCGCGCCCGGGAAATCCCGCCCGCCTCTCGGCGTGGGTCGATACCGGCGGCAAACAACGCCTCGGTGGCGTAGATGTTGccc  >  2:650101/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                          
TACACGCTCGCCCTCTGCCCCAGCTTCACTTCGCGCAACTCAGTGCCGCATACCTTGCACGGCTGCCCGCCACGCCCGTACACAAACAGTTCCTGCTGGAAGTACCCCGGCTGCCCGTCGCCACCGATAAAGTCGCGCAGGGTGGTACCGCCCTGCTCGATGGCCGCCGCCAGCACCCGTTTGATCTCGATCGCCAGTTTCAGGTAGCGCGCCCGGGAAATCCCGCCCGCCTCTCGGCGTGGGTCGATACCGGCGGCAAACAACGCCTCGGTGGCGTAGATGTTGCCC  >  NC_002947/5846097‑5846384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑