Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,916,404	G→A	8.7%	A160V (GCA→GTA)	argE ←	acetylornithine deacetylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,916,404	0	G	A	8.7%	121.9 / 3.4	46	A160V (GCA→GTA)	argE	acetylornithine deacetylase
Reads supporting (aligned to +/- strand):  ref base G (22/20);  new base A (2/2);  total (24/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.51e-01

CATGACCGCATGCATGGCCTCCATGGCGCTGCGGCCCAGGCTTGGGTCCGACGAATGGCCGCTGCGACCGAGGATGTCGATGCGGTCCATGAGGATGCCCTTGTGCATGCGGATCGGCCGCAGCCCGGTAGGCTCACCGAGGATCGCTGCACGGCCCAGTGGCTGGCCGGCCTCGGCCAGGGCACGGGCGCCGGACATGGAGCTTTCTTCGTCGCAGGTGGCAAGGATCAGCAGCGGCTCCTTGAAGTCGTGCTCCAGCAGCGGGATGACCGCTTCGATGACCAGGGCGAAGAAGCC  >  NC_002947/5916256‑5916552                                                                                                                                                     |                                                                                                                                                     cATGACCGCATGCATGGCCTCCATGGCGCTGCGGCCCAGGCTTGGGTCCGACGAATGGCCGCTGCGACCGAGGATGTCGATGCGGTCCATGAGGATGCCCTTGTGCATGCGGATCGGCCGCAGCCCGGTAGGCTCACCGCGGATCGCTGc                                                                                                                                                     >  1:109092/1‑150 (MQ=255)        gcatgcatGGCCTCCATGGCGCTGCGGCCCAGGCTTGGGTCCGACGAATGGCCGCTGCGACCGAGGATGTCGATGCGGTCCATGAGGATGCCCTTGTGCATGCGGATCGGCCGCAGCCCGGTAGGCTCACCGAGGATCGCTGCACGGccc                                                                                                                                              >  2:633217/1‑150 (MQ=255)             catgGCCTCCATGGCGCTGCGGCCCAGGCTTGGGTCCGACGAATGGCCGCTGCGACCGAGGATGTCGATGCGGTCCATGAGGATGCCCTTGTGCATGCGGATCGGCCGCAGCCCGGTAGGCTCACCGAGGATCGCTGCACGGCCCAGtgg                                                                                                                                         <  2:912612/150‑1 (MQ=255)             catgGCCTCCATGGCGCTGCGGCCCAGGCTTGGGTCCGACGAATGGCCGCTGCGACCGAGGATGTCGATGCGGTCCATGAGGATGCCCTTGTGCATGCGGATCGGCCGCAGCCCGGTAGGCTCACCGAGGATCGCTGCACGGCCCAGtgg                                                                                                                                         >  2:463052/1‑150 (MQ=255)                 gCCTCCATGGCGCTGCGGCCCAGGCTTGGGTCCGACGAATGGCCGCTGCGACCGAGGATGTCGATGCGGTCCATGAGGATGCCCTTGTGCATGCGGATCGGCCGCAGCCCGGTAGGCTCACCGAGGATCGCTGCACGGCCCAGtggctgg                                                                                                                                     <  1:1105259/150‑1 (MQ=255)                    tCCATGGCGCTGCGGCCCAGGCTTGGGTCCGACGAATGGCCGCTGCGACCGAGGATGTCGATGCGGTCCATGAGGATGCCCTTGTGCATGCGGATCGGCCGCAGCCCCGTAGGCTCACCGAGGATCGCTGCACGGCCCAGTGGCTggccg                                                                                                                                  <  2:740319/150‑1 (MQ=255)                       aTGGCGCTGCGGCCCAGGCTTGGGTCCGACGAATGGCCGCTGCGACCGAGGATGTCGATGCGGTCCATGAGGATGCCCTTGTGCATGCGGATCGGCCGCAGCCCGGTAGGCTCACCGAGGATCGCTGCACGGCCCAGTGGCTggccggcc                                                                                                                               >  1:855324/1‑150 (MQ=255)                       aTGGCGCTGCGGCCCAGGCTTGGGTCCGACGAATGGCCGCTGCGACCGAGGATGTCGATGCGGTCCATGAGGATGCCCTTGTGCATGCGGATCGGCCGCAGCCCGGTAGGCTCACCGAGGATCGCTGCACGGCCCAGTGGCTggccggcc                                                                                                                               <  1:495771/150‑1 (MQ=255)                         ggCGCTGCGGCCCAGGCTTGGGTCCGACGAATGGCCGCTGCGACCGAGGATGTCGATGCGGTCCATGAGGATGCCCTTGTGCATGCGGATCGGCCGCAGCCCGGTAGGCTCACCGAGGATCGCTGCACGGCCCAGTGGCTGGCCGGCCTc                                                                                                                             >  2:928281/1‑150 (MQ=255)                                  gCCCAGGCTTGGGTCCGACGAATGGCCGCTGCGACCGAGGATGTCGATGCGGTCCATGAGGATGCCCTTGTGCATGCGGATCGGCCGCAGCCCGGTAGGCTCACCGAGGATCGCTGCACGGCCCAGTGGCTGGCCGGCCTCGGCCAGGGc                                                                                                                    >  1:1066532/1‑150 (MQ=255)                                    ccAGGCTTGGGTCCGACGAATGGCCGCTGCGACCGAGGATGTCGATGCGGTCCATGAGGATGCCCTTGTGCATGCGGATCGGCCGCAGCCCGGTAGGCTCACCGAGGATCGCTGCACGGCCCAGTCATTGGCCTGGCTCGGCCAGGGCAc                                                                                                                  <  2:375899/150‑1 (MQ=255)                                              gTCCGACGAATGGCCGCTGCGACCGAGGATGTCGATGCGGTCCATGAGGATGCCCTTGTGCATGCGGATCGGCCGCAGCCCGGTAGGCTCACCGAGGATCGCTGCACGGCCCAGTGGCTGGCCGGCCTCGGCCAGGGCACGGGCGCCGGa                                                                                                        >  2:85593/1‑150 (MQ=255)                                                  gacgaATGGCCGCTGCGACCGAGGATGTCGATGCGGTCCATGAGGATGCCCTTGTGCATGCGGATCGGCCGCAGCCCGGTAGGCTCACCGAGGATCGCTGCACGGCCCAGtg                                                                                                                                          >  1:1101802/1‑112 (MQ=255)                                                  gacgaATGGCCGCTGCGACCGAGGATGTCGATGCGGTCCATGAGGATGCCCTTGTGCATGCGGATCGGCCGCAGCCCGGTAGGCTCACCGAGGATCGCTGCACGGCCCAGtg                                                                                                                                          <  2:1101802/112‑1 (MQ=255)                                                  gacgaATGGCCGCTGCGACCGAGGATGTCGATGCGGTCCATGAGGATGCCCTTGTGCATGCGGATCGGCCGCAGCCCGGTAGGCTCACCGAGGATCGCTGCACGGCCCAGTGGCTGGCCGGCCTCGGCCAGGGCACGGGCGCCGGACATg                                                                                                    >  1:217634/1‑150 (MQ=255)                                                       aTGGCCGCTGCGCAAGAGGACATTGATGCGGTCCATGAGGATGCCCTTGTGCATGCGGATCGGCCGCAGCCCGGTAGGCTCACCGAGGATCGCTGCACGGCCCAGTGGCTGGCCGGCCTCGGCCAGGGCACGGGCGCCGGACATGGAGCt                                                                                               <  2:255944/150‑1 (MQ=255)                                                           ccGCTGCGACCGAGGATGTCGATGCGGTCCATGAGGATGCCCTTGTGCATGCGGATCGGCCGCAGCCCGGTAGGCTCACCGAGGATCGCTGCACGGCCCAGTGGCTGGCCGGCCTCGGCCAGGGCACGGGCGCCGGACATGGAGCTttct                                                                                           >  1:173711/1‑150 (MQ=255)                                                              cTGCGACCGAGGATGTCGATGCGGTCCATGAGGATGCCCTTGTGCATGCGGATCGGCCGCAGCCCGGTAGGCTCACCGAGGATCGCTGCACGGCCCAGTGGCTGGCCGGCCTCGGCCAGGGCACGGGCGCCGGACATGGAGCTTTCTtcg                                                                                        <  2:712673/150‑1 (MQ=255)                                                               tGCGACCGAGGATGTCGATGCGGTCCATGAGGATGCCCTTGTGCATGCGGATCGGCCGCAGCCCGGTAGGCTCACCGAGGATCGCTGCACGGCCCAGTGGCTGGCCGGCCTCGGCCAGGGCACGGGCGCCGGACATGGAGCTTTCTtcgt                                                                                       <  1:926236/150‑1 (MQ=255)                                                                   aCCGAGGATGTCGATGCGGTCCATGAGGATGCCCTTGTGCATGCGGATCGGCCGCAGCCCGGTAGGCTCACCGAGGATCGCTGCACGGCCCAGTGGCTGGCCGGCCTCGGCCAGGGCACGGGCGCCGGACATGGAGCTTTCTTCGTCGCa                                                                                   >  2:688363/1‑150 (MQ=255)                                                                       atgATGTCGATGCGGTCCATGAGGATGCCCTTGTGCATGCGGATCGGCCGCAGCCCGGTAGGCTCACCGAGGATCGCTGCACGGCCCAGTGGCTGGCCGGCCTCGGCCAGGGCACGGGCGCCGGACATGGAGCTTTCTTCGTCGCAGGTg                                                                               >  2:21075/3‑150 (MQ=255)                                                                             tCGATGCGGTCCATGAGGATGCCCTTGTGGATGCGGATCGGCCGCAGCCCGGTAGGCTCACCGAGGATCGCTGCACGGCCCAGTGGCTGGCCGGCCTCGGCCAGGGCACGGGCGCCGGACATGGAGCTTTCTTCGTCGCAGGTGGCAAgg                                                                         >  1:274404/1‑150 (MQ=255)                                                                              cGATGCGGTCCATGAGGATGCCCTTGTGCATGCGGATCGGCCGCAGCCCGGTAGGCTCACCGAGGATCGCTGCACGGCCCAGTGGCTGGCCGGCCTCGGCCAGGGCACGGGCGCCGGACATGGAGCTTTCTTCGTCGCAGGTGGCAAGGa                                                                        <  1:463052/150‑1 (MQ=255)                                                                                aTGCGGTCCATGAGGATGCCCTTGTGCATGCGGATCGGCCGCAGCCCGGTAGGCTCACCGAGGATCGCTGCACGGCCCAGTGGCTGGCCGGCCTCGGCCAGGGCACGGGCGCCGGACATGGAGCTTTCTTCGTCGCAGGTGGCAAGGATc                                                                      <  1:83857/150‑1 (MQ=255)                                                                                    ggTCCATGAGGATGCCCTTGTGCATGCGGATCGGCCGCAGCCCGGTAGGCTCACCGAGGATCGCTGCACGGCCCAGTGGCTGGCCGGCCTCGGCCAGGGCACGGGCGCCGGACATGGAGCTTTCTTCGTCGCAGGTGGCAAGGATcagca                                                                  >  1:378250/1‑150 (MQ=255)                                                                                            aGGATGCCCTTGTGCATGCGGATCGGCCGCAGCCCGGTAGGCTCACCGAGGATCGCTGCACGGCCCAGTGGCTGGCCGGCCTCGGCCAGGGCACGGGCGCCGGACATGGAGCTTTCTTCGTCGCAGGTGGCAAGGATCAGCAGCggacac                                                          <  2:461298/150‑5 (MQ=255)                                                                                              gATGCCCTTGTGCATGCGGATCGGCCGCAGCCCGGTAGGCTCACCGAGGATCGCTGCACGGCCCAGTGGCTGGCCGGCCTCGGCCAGGGCACGGGCGCCGGACATGGAGCTTTCTTCGTCGCAGGTGGCAAGGATCAGCAGCGGCTCCtt                                                        <  1:633217/150‑1 (MQ=255)                                                                                                    cTTGTGCATGCGGATCGGCCGCAGCCCGGTAGGCTCACCGAGGATCGCTGCACGGCCCAGTGGCTGGCCGGCCTCGGCCAGGGCACGGGCGCCGGACATGGAGCTTTCTTCGTCGCAGGTGGCAAGGATCAGCAGCGGCTCCTTGAAGTc                                                  <  2:82866/150‑1 (MQ=255)                                                                                                          cATGCGGATCGGCCGCAGCCCGGTAGGCTCACCGAGGATCGCTGCACGGCCCAGTGGCTGGCCGGCCTCGGCCAGGGCACGGGCGCCGGACATGGAGCTTTCTTCGTCGCAGGTGGCAAGGATCAGCAGCGGCTCCTTGAAGTCGTGCTc                                            <  1:85593/150‑1 (MQ=255)                                                                                                               ggATCGGCCGCAGCCCGGTAGGCTCACCGAGGATCGCTGCACGGCCCAGTGGCTGGCCGGCCTCGGCCAGGGCACGGGCGCCGGACATGGAGCTTTCTTCGTCGCAGGTGGCAAGGATCAGCAGCGGCTCCTTGAAGTCGTGCTCcagca                                       <  2:855324/150‑1 (MQ=255)                                                                                                                  tCGGCCGCAGCCCGGTAGGCTCACCGAGGATCGCTGCACGGCCCAGTGGCTGGCCGGCCTCGGCCAGGGCACGGGCGCCGGACATGGAGCTTTCTTCGTCGCAGGTGGCAAGGATCAGCAGCGGCTCCTTGAAGTCGTGCTCCAGCAGCg                                    <  2:227594/150‑1 (MQ=255)                                                                                                                     gCCGCAGCCCGGTAGGCTCACCGAGGATCGCTGCACGGCCCAGTGGCTGGCCGGCCTCGGCCAGGGCACGGGCGCCGGACATg                                                                                                    >  1:1044992/1‑83 (MQ=255)                                                                                                                     gCCGCAGCCCGGTAGGCTCACCGAGGATCGCTGCACGGCCCAGTGGCTGGCCGGCCTCGGCCAGGGCACGGGCGCCGGACATg                                                                                                    <  2:1044992/83‑1 (MQ=255)                                                                                                                           gCCCGGTAGGCTCACCGAGGATCGCTGCACGGCCCAGTGGCTGGCCGGCCTCGGCCAGGGCACGGGCGCCGGACATGGAGCTTTCTTCGTCGCAGGTGGCAAGGATCAGCAGCGGCTCCTTGAAGTCGTGCTCCAGCAGCGGGATGa                              <  2:685623/147‑1 (MQ=255)                                                                                                                           gCCCGGTAGGCTCACCGAGGATCGCTGCACGGCCCAGTGGCTGGCCGGCCTCGGCCAGGGCACGGGCGCCGGACATGGAGCTTTCTTCGTCGCAGGTGGCAAGGATCAGCAGCGGCTCCTTGAAGTCGTGCTCCAGCAGCGGGATGa                              >  1:685623/1‑147 (MQ=255)                                                                                                                                    gCTCACCGAGGATCGCTGCACGGCCCAGTGGCTGGCCGGCCTCGGCCAGGGCACGGGCGCCGGACATGGAGCTTTCTTCGTCGCAGGTGGCAAGGATCAGCAGCGGCTCCTTGAAGTCGTGCTCCAGCAGCGGGATGACCGCTTCGATGa                  >  1:1113132/1‑150 (MQ=255)                                                                                                                                     cTCACCGAGGATCGCTACACGGCCCAGTGGCTGGCCGGCCTCGGCCAGGGCACGGGCGCCGGACATGGAGCTTTCTTCGTCGCAGGTGGCaa                                                                           >  2:53815/1‑92 (MQ=255)                                                                                                                                     cTCACCGAGGATCGCTACACGGCCCAGTGGCTGGCCGGCCTCGGCCAGGGCACGGGCGCCGGACATGGAGCTTTCTTCGTCGCAGGTGGCaa                                                                           >  2:53742/1‑92 (MQ=255)                                                                                                                                     cTCACCGAGGATCGCTACACGGCCCAGTGGCTGGCCGGCCTCGGCCAGGGCACGGGCGCCGGACATGGAGCTTTCTTCGTCGCAGGTGGCaa                                                                           <  1:53742/92‑1 (MQ=255)                                                                                                                                     cTCACCGAGGATCGCTACACGGCCCAGTGGCTGGCCGGCCTCGGCCAGGGCACGGGCGCCGGACATGGAGCTTTCTTCGTCGCAGGTGGCaa                                                                           <  1:53815/92‑1 (MQ=255)                                                                                                                                      tCACCGAGGATCGCTGCACGGCCCAGTGGCTGGCCGGCCTCGGCCAGGGCACGGGCGCCGGACATGGAGCTTTCTTCGTAGCAGGTGGCAAGGATCAGCAGCGGCTCCTTGAAGTCGTGCTCCAGCAGCGGGATGACCGCTTCGATGAcc                >  1:244416/1‑150 (MQ=255)                                                                                                                                        aCCGAGGATCGCTGCACGGCCCAGTGGCTGGCCGGCCTCGGCCAGGGCACGGGCGCCGGACATGGAGCTTTCTTCGTCGCAGGTGGCAAGGATCAGCAGCGGCTCCTTGAAGTCGTGCTCCAGCAGCGGGATGACCGCTTCGATGACCAg              >  1:715876/1‑150 (MQ=255)                                                                                                                                          cGAGGATCGCTGCACGGCCCAGTGGCTGGCCGGCCTCGGCCAGGGCACGGGCGCCGGACATGGAGCTTTCTTCGTCGCAGGTGGCAAGGATCAGCAGCGGCTCCTTGAAGTCGTGCTCCAGCAGCGGGATGACCGCTTCGATGACCAggg            >  1:1129008/1‑150 (MQ=255)                                                                                                                                           gAGGATCGCTGCACGGCCCAGTGGCTGGCCGGCCTCGGCCAGGGCACGGGCGCCGGACATGGAGCTTTCTTCGTCGCAGGTGGCAAGGATCAGCAGCGGCTCCTTGAAGTCGTGCTCCAGCAGCGGGATGACCGCTTCGATGACCAGGGc           >  1:188003/1‑150 (MQ=255)                                                                                                                                              gATCGCTGCACGGCCCAGTGGCTGGCCGGCCTCGGCCAGGGCACGGGCGCCGGACATGGAGCTTTCTTCGTCGCAGGTGGCAAGGATCAGCAGCGGCTCCTTGAAGTCGTGCTCCAGCAGCGGGATGACCGCTTCGATGACCAGGGCgaa        <  2:173711/150‑1 (MQ=255)                                                                                                                                                    tGCACGGCCCAGTGGCTGGCCGGCCTCGGCCAGGGCACGGGCGCCGGACATGGAGCTTTCTTCGTCGCAGGTGGCAAGGATCAGCAGCGGCTCCTTGAAGTCGTGCTCCAGCAGCGGGATGACCGCTTCGATGACCAGGGCGAAGAAGcc  >  2:957969/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                     CATGACCGCATGCATGGCCTCCATGGCGCTGCGGCCCAGGCTTGGGTCCGACGAATGGCCGCTGCGACCGAGGATGTCGATGCGGTCCATGAGGATGCCCTTGTGCATGCGGATCGGCCGCAGCCCGGTAGGCTCACCGAGGATCGCTGCACGGCCCAGTGGCTGGCCGGCCTCGGCCAGGGCACGGGCGCCGGACATGGAGCTTTCTTCGTCGCAGGTGGCAAGGATCAGCAGCGGCTCCTTGAAGTCGTGCTCCAGCAGCGGGATGACCGCTTCGATGACCAGGGCGAAGAAGCC  >  NC_002947/5916256‑5916552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑