Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,000,005 G→A 9.0% V162M (GTG→ATG)  PP_5256 → cyclic nucleotide‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,000,0050GA 9.0% 114.3 / 2.9 45V162M (GTG→ATG) PP_5256cyclic nucleotide‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (21/19);  new base A (2/2);  total (23/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.48e-01

TTGATCTTCCTCGCCGCCGTGGTCGCGGCCGCAGGCTACGTGCTGCAACTGCCGGTAAAGGGCCTGCTGGCCACCTCCGGGGTAATGGCCATCGTCATCGGCCTGGCGCTGCAGAGCACACTGGCTGACGTCTTCAGCGGCATCGTGCTGAACACCACGCGGCCCTATCAGATTGGCGACTCAATCTCCATCGACGGCACCGAAGGCAAGGTCCTGGATATCGACTGGCGCGCCACGCGCCTGCTGACCGGAACCGGCAGCCTGGCGGTGATCCCCAACTCGGTAGCG  >  NC_002947/5999861‑6000148
                                                                                                                                                |                                                                                                                                               
ttGATCTTCCTCGCCGCCGTGGTCGCGGCCGCAGGCTACGTGCTGCAACTGCCGGTAAAGGGCCTGCTGGCCACCTCCGGGGTAATGGCCATCGTCATCGGCCTGGCGCTGCAGAGCACACTGGCTGACGTCTTCAGCGGCATCGTGCTg                                                                                                                                            <  1:322235/150‑1 (MQ=255)
                     gTCGCGGCCGCAGGCTACGTGCTGCAACTGCCGGTAAAGGGCCTGCTGGCCACCTCCGGGGTAATGGCCATCGTCATCGGCCTGGCGCTGCAGAGCACACTGGATGACGTCTTCAGCGGCATCGTGc                                                                                                                                              >  2:832481/1‑127 (MQ=255)
                     gTCGCGGCCGCAGGCTACGTGCTGCAACTGCCGGTAAAGGGCCTGCTGGCCACCTCCGGGGTAATGGCCATCGTCATCGGCCTGGCGCTGCAGAGCACACTGGATGACGTCTTCAGCGGCATCGTGc                                                                                                                                              <  1:832481/127‑1 (MQ=255)
                      tCGCGGCCGCAGGCTACGTGCTGCAACTGCCGGTAAAGGGCCTGCTGGCCACCTCCGGGGTAATGGCCATCGTCATCGGCCTGGCGCTGCAGAGCACACTGGCGGACGTCTTCAGCGGCATCGTGCTGAACACCACGCGGCCCTATCAGa                                                                                                                      >  1:242827/1‑150 (MQ=255)
                        gcgGCCGCAGGCTACGTGCTGCAACTGCCGGTAAAGGGCCTGCTGGCCACCTCCGGGGTAATGGCCATCGTCATCGGCCTGGCGCTGCAGAGCACACTGGCTGACGTCTTCAGCGGCATCGTGCTGAACACCACGCGGCCCTATCAGAtt                                                                                                                    >  2:74296/1‑150 (MQ=255)
                        gcgGCCGCAGGCTACGTGCTGCAACTGCCGGTAAAGGGCCTGCTGGCCACCTCCGGGGTAATGGCCATCGTCATCGGCCTGGCGCTGCAGAGCACACTGGCTGACGTCTTCAGCGGCATCGTGCTGAACACCACGCGGCCCTATCAGAtt                                                                                                                    <  1:973466/150‑1 (MQ=255)
                           gCCGCAGGCTACGTGCTGCAACTGCCGGTAAAGGGCCTGCTGGCCACCTCCGGGGTAATGGCCATCGTCATCGGCCTGGCGCTGCAGAGCACACTGGCTGACGTCTTCAGCGGCATCGTGCTGAACACCACGCGGCCCTATCAGATTGGc                                                                                                                 <  1:440459/150‑1 (MQ=255)
                             cGCAGGCTACGTGCTGCAACTGCCCGTAAAGGGCCTGCTGGCCACCTCCGGGGTAATGGCCATCGTCATCGGCCTGGCGCTGCAGAGCACACTGGCTGACGTCTTCAGCGGCATCGTGCTGAACACCACGCGGCCCTATCAGATTGGCGa                                                                                                               <  2:181532/150‑1 (MQ=255)
                              gCAGGCTACGTGCTGCAACTGCCGGTAAAGGGCCTGCTGGCCACCTCCGGGGTAATGGCCATCGTCATCGGCCTGGCGCTGCAGAGCACACTGGCTGACGTCTTCAGCGGCATCGTGCTGAACACCACGCGGCCCTATCAGATTGGCGAc                                                                                                              >  2:689893/1‑150 (MQ=255)
                              gCAGGCTACGTGCTGCAACTGCCGGTAAAGGGCCTGCTGGCCACCTCCGGGGTAATGGCCATCGTCATCGGCCTGGCGCTGCAGAGCACACTGGCTGACGTCTTCAGCGGCATCGTGCTGAACACCACGCGGCCCTATCAGATTGGCGAc                                                                                                              >  1:891804/1‑150 (MQ=255)
                                   ccactggctccAACTGCCGGTAAAGGTCCTGCTGGCCACCTCCGGGGTAATGGCCATCGTCATCGGCCTGGCGCTGCAGAGCACACTGGCTGACGTCTTCAGCGGCATCGTGCTGAACACCACGCGGCCCTATCAGATTGGCGACTCAAt                                                                                                         <  1:44205/144‑1 (MQ=255)
                                     aCGGGCTGCAACGGCCGGTAAAGGGCCTGCTGGCCACCTCCGGGGGAATGGCCATCGTCATCGGCCGGGCGCTGCAGAGCACACTGGCTGACGTCTTCAGCGGCATCGTGCTGAACACCACGCGGCCCTATCAGATTGGCGACTCAAgct                                                                                                       <  2:54970/150‑1 (MQ=255)
                                           tgcAACTGCCGGTAAAGGGCCTGCTGGCCACCTCCGGGGTAATGGCCATCGTCATCGGCCTGGCGCTGCAGAGCACACTGGCTGACGTCTTCAGCGGCATCGTGCTGAACACCACGCGGCCCTATCAGATTGGCGACTCAATCTCCATCg                                                                                                 >  1:1085947/1‑150 (MQ=255)
                                                cTGCCGGTAAAGGGCCTGCTGGCCACCTCCGGGGTAATGGCCATCGTCATCGGCCTGGCGCTGCAGAGCACACTGGCTGACGTCTTCAGCGGCATCGTGCTGAACACCACGCGGCCCTATc                                                                                                                         <  2:983571/121‑1 (MQ=255)
                                                cTGCCGGTAAAGGGCCTGCTGGCCACCTCCGGGGTAATGGCCATCGTCATCGGCCTGGCGCTGCAGAGCACACTGGCTGACGTCTTCAGCGGCATCGTGCTGAACACCACGCGGCCCTATc                                                                                                                         >  1:983571/1‑121 (MQ=255)
                                                cTGCCGGTAAAGGGCCTGCTGGCCACCTCCGGGGTAATGGCCATCGTCATCGGCCTGGCGCTGCAGAGCACACTGGCTGACGTCTTCAGCGGCATCATGCTGAACACCACGCGGCCCTATCAGATTGGCGAc                                                                                                              <  2:229299/132‑1 (MQ=255)
                                                cTGCCGGTAAAGGGCCTGCTGGCCACCTCCGGGGTAATGGCCATCGTCATCGGCCTGGCGCTGCAGAGCACACTGGCTGACGTCTTCAGCGGCATCATGCTGAACACCACGCGGCCCTATCAGATTGGCGAc                                                                                                              <  2:229031/132‑1 (MQ=255)
                                                cTGCCGGTAAAGGGCCTGCTGGCCACCTCCGGGGTAATGGCCATCGTCATCGGCCTGGCGCTGCAGAGCACACTGGCTGACGTCTTCAGCGGCATCATGCTGAACACCACGCGGCCCTATCAGATTGGCGAc                                                                                                              >  1:229031/1‑132 (MQ=255)
                                                cTGCCGGTAAAGGGCCTGCTGGCCACCTCCGGGGTAATGGCCATCGTCATCGGCCTGGCGCTGCAGAGCACACTGGCTGACGTCTTCAGCGGCATCATGCTGAACACCACGCGGCCCTATCAGATTGGCGAc                                                                                                              >  1:229299/1‑132 (MQ=255)
                                                 tGCCGGTAAAGGGCCTGCTGGCCACCTCCGGGGTAATGGCCATCGTCATCGGCCTGGCGCTGCAGAGCACACTGGCTGACGTCTTCAGCGGCATCGTGCTGAACACCACGCGGCCCTATCAGATTGGCGACTCAATCTCCATCGACGGCa                                                                                           >  1:305821/1‑150 (MQ=255)
                                                     ggTAAAGGGCCTGCTGGCCACCTCCGGGGTAATGGCCATCGTCATCGGCCTGGCGCTGCAGAGCACACTGGCTGACGTCTTCAGCGGCATCGTGc                                                                                                                                              >  2:248022/1‑95 (MQ=255)
                                                     ggTAAAGGGCCTGCTGGCCACCTCCGGGGTAATGGCCATCGTCATCGGCCTGGCGCTGCAGAGCACACTGGCTGACGTCTTCAGCGGCATCGTGCTGAACACCACGCGGCCCTAGCAGATTGGCGACTCAATCTCCATCGACGGCACCGa                                                                                       >  2:784454/1‑150 (MQ=255)
                                                     ggTAAAGGGCCTGCTGGACACCGCCGGGGTAATGGCCATCGTCATCGGCCTGGCGCTGCAGAGCACACTGGCTGACGTCTTCAGCGGCATCGTGc                                                                                                                                              <  1:248022/95‑1 (MQ=255)
                                                       tAAAGGGCCTGCTGGCCACCTCCGGGGTAATGGCCATCGTCATCGGCCTGGCGCTGCAGAGCACACTGGCTGACGTCTTCAGCGGCATCGTGCTGAACACCACGCGGCCCTATCAGATTGGCGCCTCAATCTCCATCGACGGCACCGAAg                                                                                     <  2:530211/150‑1 (MQ=255)
                                                          aGGGCCTGCTGGCCACCTCCGGGGTAATGGCCATCGTCATCGGCCTGGCGCTGCAGAGCACACTGGCTGACGTCTTCAGCGGCATCGTGCTGAACACCACGCGGCCCTATCAGATTGGCGACTCAATCTCCATCGACGGCACCGAAGGCa                                                                                  >  2:894871/1‑150 (MQ=255)
                                                                       cACCTCCGGGGTAATGGCCATCGTCATCGGCCTGGCGCTGCAGAGCACACTGGCTGACGTCTTCAGCGGCCTCGTGCTGAACACCACGCGGCCCTATCAGATTGGCGACTCAATCTCCATCGACGGCACCGAAGGCAAGGTCCt                                                                           <  2:913474/144‑1 (MQ=255)
                                                                       cACCTCCGGGGTAATGGCCATCGTCATCGGCCTGGCGCTGCAGAGCACACTGGCTGACGTCTTCAGCGGCATCGTGCTGAACACCACGCGGCCCTATCAGATTGGCGACTCAATCTCCATCGACGGCACCGAAGGCAAGGTCCt                                                                           >  1:913474/1‑144 (MQ=255)
                                                                       cACCTCCGGGGTAATGGCCATCGTCATCGGCCTGGCGCTGCAGAGCACACTGGCTGACGTCTTCAGCGGCATCGTGCTGAACACCACGCGGCCCTATCAGATTGGCGACTCAATCTCCATCGACGGCACCGAAGGCAAGGTCCTGGatac                                                                     >  2:85886/1‑149 (MQ=255)
                                                                           tCCGGGGTAATGGCCATCGTCATCGGCCTGGCGCTGCAGAGCACACTGGCTGACGTCTTCAGCGGCATCGTGCTGAACACCACGCGGCCCTATCAGATTGGCGACTCAATCTCCATCGACGGCACCGAAGGCAAGGTCCTGGATATCGAc                                                                 >  2:372837/1‑150 (MQ=255)
                                                                                     tGGCCATCGTCATCGGCCTGGCGCTGCAGAGCACACTGGCTGACGTCTTCAGCGGCATCGTGCTGAACACCACGCGGCCCTATCAGATTGGCGACTCAATCTCCATCGACGGCACCGAAGGCAAGGTCCTGGATATCGACTGGCGCGCCa                                                       <  1:496561/150‑1 (MQ=255)
                                                                                       gCCATCGTCATCGGCCTGGCGCTGCAGAGCACACTGGCTGACGTCTTCAGCGGCATCGTGCTGAACACCACGCGGCCCTATCAGATTGGCGACTCAATCTCCATCGACGGCACCGAAGGCAAGGTCCTGGATATCGACTGGCGCGCCAcg                                                     >  2:129624/1‑150 (MQ=255)
                                                                                        ccATCGTCATCGGCCTGGCGCTGCAGAGCACACTGGCTGACGTCTTCAGCGGCATCGTGCTGAACACCACGCGGCCCTATCAGATTGGCGACTCAATCTCCATCGACGGCACCGAAGGCAAGGTCCTGGATATCGACTGGCGCGCCAcgc                                                    >  2:97566/1‑150 (MQ=255)
                                                                                         cATCGTCATCGGCCTGGCGCTGCAGAGCACACTGGCTGACGTCTTCAGCGGCATCGTGCTGAACACCACGCGGCCCTATCAGATTGGCGACTCAATCTCCATCGACGGCACCGAAGGCAAGGTCCTGGATATCGACTGgcgcgtggcgcg                                                   >  1:56185/1‑143 (MQ=255)
                                                                                          aTCGTCATCGGCCTGGCGCTGCAGAGCACACTGGCTGACGTCTTCAGCGGCATCGTGCTGAaca                                                                                                                                        <  2:1103470/64‑1 (MQ=255)
                                                                                          aTCGTCATCGGCCTGGCGCTGCAGAGCACACTGGCTGACGTCTTCAGCGGCATCGTGCTGAaca                                                                                                                                        >  1:1103470/1‑64 (MQ=255)
                                                                                            cGTCATCGGCCTGGCGCTGCAGAGCACACTGGCTGACGTCTTCAGCGGCATCGTGCTGAACACCACGCGGCCCTATCAGATTGGCGACTCAATCTCCATCGACGGCACCGAAGGCAAGGTCCTGGATATCGACTGGCGCGCCACGCGCct                                                <  1:894871/150‑1 (MQ=255)
                                                                                                        ggCGCTGCAGAGCACACTGGCTGACGTCTTCAGCGGCATCGTGCTGAACACCACGCGGCCCTATCAGATTGGCGACTCAATCTCCATCGACGGCACCGAAGGCAAGGTCCTGGATATCGACTGGCGCGCCACGCGCCTGCTGACCGGAAc                                    <  2:824610/150‑1 (MQ=255)
                                                                                                             tGCAGAGCACACTGGCTGACGTCTTCAGCGGCATCGTGCTGAACACCACGCGGCCCTATCAGATTGGCGACTCAATCTCCATCGACGGCACCGAAGGCAAGGTCCTGGATATCGACTGGCGCGCCACGCGCCTGCTGACCGGAACCGGCa                               <  1:129624/150‑1 (MQ=255)
                                                                                                                      cacTGGCTGACGTCTTCAGCGGCATCTTTCTGAACACCACGCGGCCCTATCAGATTGGCGACTCAATCTCCATCGACGGCACCGAAGGCAAGGTCCTGGATATCGACTGGCGCGCCACGCGCCTGCTGACCGGAACCGGCAGCCTGGCgg                      <  1:786365/150‑1 (MQ=255)
                                                                                                                           gCTGACGTCTTCAGCGGCATCGTGCTGAACACCACGCGGCCCTATCAGATTGGCGACTCAATCTCCATCGACGGCACCGAAGGCAAGGTCCTGGATATCGACTGGCGCGCCACGCGCCTGCTGACCGGAACCGGCAGCCTGGCGGTGATc                 <  2:305821/150‑1 (MQ=255)
                                                                                                                            cTGACGTCTTCAGCGGCATCGTGCTGAACACCACGCGGCCCTATCAGATTGGCGACTCAATCTCCATCGACGGCACCGAAGGCAAGGTCCTGGATATCGACTGGCGCGCCACGCGCCTGCTGACCGGAACCGGCAGCCTGGCGGTGATcc                >  1:1060824/1‑150 (MQ=255)
                                                                                                                                    ttCAGCGGCATCGTGCTGAACACCACGCGGCCCTATCAGATTGGCGACTCAATCTCCATCGACGGCACCGAAGGCAAGGTCCTGGATATCGACTGGCGCGCCACGCGCCTGCTGACCGGAACCGGCAGc                             >  1:1061114/1‑129 (MQ=255)
                                                                                                                                    ttCAGCGGCATCGTGCTGAACACCACGCGGCCCTATCAGATTGGCGACTCAATCTCCATCGACGGCACCGAAGGCAAGGTCCTGGATATCGACTGGCGCGCCACGCGCCTGCTGACCGGAACCGGCAGc                             <  2:1061114/129‑1 (MQ=255)
                                                                                                                                     tCAGCGGCATCGTGCTGAACACCACGCGGCCCTATCAGATTGGCGACTCAATCTCCATCGACGGCACCGAAGGCAAGGTCCTGGATATCGACTGGCGCGCCACGCGCCTGCTGACCGGAACCGGCAGCCTGGCGGTGATCCCCAACTCgg       >  1:421191/1‑150 (MQ=255)
                                                                                                                                          ggCATCGTGCTGAACACCACGCGGCCCTATCAGATTGGCGACTCAATCTCCATCGACGGCACCGAAGGCAAGGTCCTGGATATCGACTGGCGCGCCACGCGCCTGCTGACCGGAACCGGCAGCCTGGCGGTGATCCCCAACTCGGTAGCg  <  1:825572/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                               
TTGATCTTCCTCGCCGCCGTGGTCGCGGCCGCAGGCTACGTGCTGCAACTGCCGGTAAAGGGCCTGCTGGCCACCTCCGGGGTAATGGCCATCGTCATCGGCCTGGCGCTGCAGAGCACACTGGCTGACGTCTTCAGCGGCATCGTGCTGAACACCACGCGGCCCTATCAGATTGGCGACTCAATCTCCATCGACGGCACCGAAGGCAAGGTCCTGGATATCGACTGGCGCGCCACGCGCCTGCTGACCGGAACCGGCAGCCTGGCGGTGATCCCCAACTCGGTAGCG  >  NC_002947/5999861‑6000148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑