| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 6,043,722 | T→A | 11.4% | intergenic (+18/+16) | gmk → / ← PP_5297 | deoxyguanylate kinase/guanylate kinase/amino acid transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 6,043,722 | 0 | T | A | 11.4% | 124.4 / 7.8 | 44 | intergenic (+18/+16) | gmk/PP_5297 | deoxyguanylate kinase/guanylate kinase/amino acid transporter |
| Reads supporting (aligned to +/- strand): ref base T (23/16); new base A (2/3); total (25/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01 | |||||||||||
TACGTCATCATCAATGATGATTTCGACACGGCGCTGGCAGACTTGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAGGCCGATGCCAAGCACCGAGATGATCGCGGCAATCACGAACACCAGC > NC_002947/6043576‑6043870 | tACGTCATCATCAATGATGATTTCGACACGGCGCTGGCAGACTTGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGc > 2:940945/1‑150 (MQ=255) aCGTCATCATCAATGATGATTTCGACACGGCGCTGGCAGACTTGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCa < 2:212828/150‑1 (MQ=255) catcaATGATGATTTCGACACGGCGCTGGCAGACTTGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCAATGCAGCCCCat < 1:387927/150‑2 (MQ=255) atcaATGATGATTTCGACACGGCGCTGGCAGACTTGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAAt < 1:763331/150‑1 (MQ=255) atcaATGATGATTTCGACACGGCGCTGGCAGACTTGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCAATGCAGCCCCatc < 1:267555/150‑3 (MQ=255) atcaATGATGATTTCGACACGGCGCTGGCAGACTTGAAAGCAGTGCTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAAt < 1:239582/150‑1 (MQ=255) tcaATGATGTTTTCGACACGGCGCTGGCAGACTTGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATGGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATg < 2:98015/150‑1 (MQ=255) atgatgATTTCGACACGGCGCTGGCAGACTTGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGtt < 1:458944/150‑1 (MQ=255) tgatgaTTTCGACACGGCGCTGGCAGACTTGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAATTAATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGttt > 1:674366/1‑150 (MQ=255) gatTTCGACACGGCGCTGGCAGACTTGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTc < 2:363279/150‑1 (MQ=255) atTTCGACACGGCGCTGGCAGACTTGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTcc > 2:49271/1‑150 (MQ=255) ttCGACACGGCGCTGGCAGACTTGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTg < 1:84195/150‑1 (MQ=255) gACACGGCGCTGGCAGACTTGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAg > 2:164401/1‑150 (MQ=255) cAGACTTGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGcc > 2:476111/1‑150 (MQ=255) ttGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCctgctg > 1:706455/1‑150 (MQ=255) gaGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAgg < 1:2561/149‑1 (MQ=255) tgtTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTa > 1:556856/1‑150 (MQ=255) gCTCGAATCGGCCGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCg > 2:197389/1‑150 (MQ=255) aaTCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGaa < 1:164401/150‑1 (MQ=255) tgAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGcgca > 2:871932/1‑150 (MQ=255) gaagCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGcgcacgca > 1:443991/1‑150 (MQ=255) aagCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCCATTTAGGTTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCAt < 1:476111/150‑1 (MQ=255) cagcagcGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGa > 1:1014976/1‑150 (MQ=255) cagcGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAg > 1:169051/1‑150 (MQ=255) aTTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAGGCCGa < 1:940945/150‑1 (MQ=255) ttGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGCGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAGGCCGAt < 2:771114/150‑1 (MQ=255) tCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAGGCCGATGCCa > 2:667576/1‑150 (MQ=255) aaGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGa < 1:827909/98‑1 (MQ=255) aaGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGa > 2:827909/1‑98 (MQ=255) gCAAATGATCGGCTGAAGATCGAGGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAGGCCGATGCCAAGCa > 1:830937/1‑150 (MQ=255) aTGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCCGGCCGATGCCAAGCACCga > 2:478495/1‑150 (MQ=255) ggCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAGGCCGATGCCAAGCACCGAgatgat > 1:113085/1‑150 (MQ=255) tGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAGGCCGATGCCAAGCACCGAGATGATcgc < 1:49271/150‑1 (MQ=255) gATCGATGGGGCTGCATTGCAGCCCCCATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAGGCCGATGCCAAGCACCGAGATGATCGCGGCa > 2:215807/1‑150 (MQ=255) gATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAGGCCGATGCCAAGCACCGAGATGATCGCGGCa > 2:967510/1‑150 (MQ=255) attGGGGCTGCAATGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAGGCCGATGCCaa > 2:487051/3‑126 (MQ=255) attGGGGCTGCAATGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAGGCCGATGCCaa < 1:487051/124‑1 (MQ=255) ttGGGGCTGCAATGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAGGCCGATGCCAAGCACCGAGATGATCGCGGCAATCAc > 2:117845/2‑150 (MQ=255) aTGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAGGCCGATGCCAAGCACCGAGATGATCGCGGCAATAAc > 2:737746/1‑150 (MQ=255) ggggCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAGGCCGATg < 1:964363/119‑1 (MQ=255) ggggCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAGGCCGATg > 2:964363/1‑119 (MQ=255) gCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAGGCCGATGCCAAGCACCGAGATGATCGCGGCAATCACGAaca < 2:443991/150‑1 (MQ=255) tGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAGGCCGATGCCAAGCACCGAGATGATCGCCGCAATCACGAACAcc > 2:134950/1‑150 (MQ=255) aTTGCAGCCCCAATGGTTTAGTCCTGCAGCGGAAAGGTGGATTGGTGCAGTTGGGTAGCAACCATTAGGAACCCCCGCGCACGCATGCGCGACAGCATCATCAGGCCGATGCCAAGCACCGAGACGATCGCGGCAATCACGAACAccagc > 1:300257/1‑150 (MQ=255) ttGCAGCCCCAATGGTTTGGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAg < 2:204112/64‑1 (MQ=255) ttGCAGCCCCAATGGTTTGGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAg > 1:204112/1‑64 (MQ=255) | TACGTCATCATCAATGATGATTTCGACACGGCGCTGGCAGACTTGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAGGCCGATGCCAAGCACCGAGATGATCGCGGCAATCACGAACACCAGC > NC_002947/6043576‑6043870 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |