Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,070,587 A→G 100% G38G (GGT→GGC)  PP_5324 ← two‑component system response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,070,5870AG93.3% 150.2 / 0.1 56G38G (GGT→GGC) PP_5324two‑component system response regulator
Reads supporting (aligned to +/- strand):  ref base A (3/1);  new base G (28/23);  total (31/25)
Fisher's exact test for biased strand distribution p-value = 6.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGACCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCGATACTCCTTTTGAATCCCGACTAGCC  >  NC_002947/6070447‑6070728
                                                                                                                                            |                                                                                                                                             
aCCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATa                                                                                                                                      <  2:740469/150‑1 (MQ=255)
          aCTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGa                                                                                                                            >  1:216914/1‑150 (MQ=255)
           cTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGAGGCCGTTGACAGcgtggtggga                                                                                                                           >  2:613103/1‑146 (MQ=255)
              tgggTTC‑TCAGTGCAGGCTGCTGACGGCACCAGGAAAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCAAAGGACCAGGTCGAACGCTTCCTTGACGAGCATGGCCATGGCGTTGCGGCCGTTGATAGCGTCGTCGATGGcc                                                                                                                       >  2:464354/3‑150 (MQ=255)
              cAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTGCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGACCGTTGATAGCGTCGTCGATGGc                                                                                                                        >  2:109072/1‑150 (MQ=255)
              cAGGTTCTTCAGCGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGGGCATGGCCAGGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGc                                                                                                                        <  1:324912/150‑1 (MQ=255)
               cggTTCTTAAGTGGAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGcc                                                                                                                       <  2:99765/149‑1 (MQ=255)
                     ttcAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATAccc                                                                                                                 >  1:919998/1‑150 (MQ=255)
                             aGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGc                                                                                                         <  1:753182/150‑1 (MQ=255)
                               gctgctGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCAt                                                                                                       >  2:598511/1‑150 (MQ=255)
                                 tgctgACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTa                                                                                                     <  2:773156/150‑1 (MQ=255)
                                    tgACGGCACCAGGTCAGCAGTTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCCTCGTCGATGGCCATACCCGGGAAGGCATGACGc                                                                                                  >  2:49426/1‑150 (MQ=255)
                                       cGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGa                                                                                               <  2:634761/150‑1 (MQ=255)
                                           aCCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACt                                                                                           >  2:556325/1‑150 (MQ=255)
                                              aGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGc                                                                                        >  1:738988/1‑150 (MQ=255)
                                              aGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCACGGCCATGGCCTTACACACAAAAATAACGTCCTCCGGGGCCATACCCGGGAAGGCATTACGCAGACACTTGc                                                                                        <  1:300784/150‑1 (MQ=255)
                                                gTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCgg                                                                                      <  1:613103/150‑1 (MQ=255)
                                                    gCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCg                                                                                       <  1:472845/145‑1 (MQ=255)
                                                    gCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCg                                                                                       >  2:472845/1‑145 (MQ=255)
                                                     gaaTTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAg                                                                                 >  1:771101/2‑150 (MQ=255)
                                                      aaTTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGa                                                                                                                            <  1:899647/106‑1 (MQ=255)
                                                      aaTTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGa                                                                                                                            >  2:899647/1‑106 (MQ=255)
                                                      aaTTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGa                                                                                >  1:255964/1‑150 (MQ=255)
                                                          ccAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCAc                                                                            <  2:216914/150‑1 (MQ=255)
                                                              gCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGAt                                                                        >  2:133734/1‑150 (MQ=255)
                                                               ccAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATa                                                                       >  1:1015478/1‑150 (MQ=255)
                                                                  gACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGcc                                                                                                                       >  2:369616/1‑99 (MQ=255)
                                                                  gACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGcc                                                                                                                       <  1:369616/99‑1 (MQ=255)
                                                                        tCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGtt                                                                                                                                          >  1:944564/1‑74 (MQ=255)
                                                                        tCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGtt                                                                                                                                          <  2:944564/74‑1 (MQ=255)
                                                                          cGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTc                                                            >  1:477387/1‑150 (MQ=255)
                                                                             cATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATc                                                         <  1:556325/150‑1 (MQ=255)
                                                                                 tCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACgg                                                                  >  2:70884/1‑137 (MQ=255)
                                                                                 tCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACgg                                                                  <  1:70884/137‑1 (MQ=255)
                                                                                  cccAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGcgtc                                                                                                                                 <  2:41089/73‑1 (MQ=255)
                                                                                  cccAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGcgtc                                                                                                                                 >  1:41089/1‑73 (MQ=255)
                                                                                   ccAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGAc                                                   >  2:555339/1‑150 (MQ=255)
                                                                                   ccAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGACCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCagggc                                                   >  1:331160/1‑146 (MQ=255)
                                                                                       tCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGACCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGc                                               >  1:49974/1‑150 (MQ=255)
                                                                                         gCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGGTGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGcac                                             >  2:679860/1‑150 (MQ=255)
                                                                                           acaGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATATTCTGGGCGTCATCCACGACCAGCACAt                                           >  1:550554/1‑150 (MQ=255)
                                                                                                aCCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACt                                      <  1:598511/150‑1 (MQ=255)
                                                                                                   gggTCGAACGCTTCCTTGCCGCGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACCTGCCGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTa                                   <  2:969220/149‑1 (MQ=255)
                                                                                                        gAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCAt                              >  1:1045748/1‑150 (MQ=255)
                                                                                                         aaCGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGACCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATc                             <  1:109072/150‑1 (MQ=255)
                                                                                                          aCGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCg                            >  1:63506/1‑150 (MQ=255)
                                                                                                            gCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCGAt                          >  2:119141/1‑150 (MQ=255)
                                                                                                              ttCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCATACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCGATAc                        <  1:1070459/150‑1 (MQ=255)
                                                                                                                  ttGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACCTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTGCTCATCGATACTCCt                    <  2:1015478/150‑1 (MQ=255)
                                                                                                                     ccGATCATGGCCATGTCCTTGCTGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCGATACTCCtttt                 <  1:464354/150‑1 (MQ=255)
                                                                                                                           atggccatggccTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCGATACTCCTTTTGAATcc           <  2:919998/150‑1 (MQ=255)
                                                                                                                                  tggccTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCGATACTCCttt                  <  1:1076542/136‑1 (MQ=255)
                                                                                                                                  tggccTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCGATACTCCttt                  >  2:1076542/1‑136 (MQ=255)
                                                                                                                                  tggccTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCGATACTCCTTTTGAATCCCGACTAg    >  1:783441/1‑150 (MQ=255)
                                                                                                                                   ggccTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCGATACTCCTTTTGAATCCCGACTAGc   <  2:945607/150‑1 (MQ=255)
                                                                                                                                    gccTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCGATACTCCTTTTGAATCCCGACTAGcc  >  2:899002/1‑150 (MQ=255)
                                                                                                                                            |                                                                                                                                             
ACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGACCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCGATACTCCTTTTGAATCCCGACTAGCC  >  NC_002947/6070447‑6070728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑