| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 6,104,098 | G→A | 8.5% | intergenic (+20/‑94) | PP_5353 → / → PP_5354 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 6,104,098 | 0 | G | A | 8.5% | 128.6 / 2.8 | 47 | intergenic (+20/‑94) | PP_5353/PP_5354 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (20/23); new base A (2/2); total (22/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01 | |||||||||||
CTTCCGTGGCGTGTCGAAGAATTCGCGCTTCGACCAGGGCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATCGAACAACTCCGTGAAGCGAATGAGCCGGTACCGGTGCC > NC_002947/6103952‑6104244 | cTTCCGTGGCGTGTCGAAGAATTCGCGCTTCGACCAGGGCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAAc > 1:133374/1‑150 (MQ=255)cTTCCGTGGCGTGTCGAAGAATTCGCGCTTCGACCAGGGCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAAAAc > 2:1131162/1‑150 (MQ=255) gTGGCGTGTCGAAGAATTCGCGCTTCGACCAGGGCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAAAACCCCgg < 1:1131162/150‑1 (MQ=255) gaagaaTTCGCGCTTCGACCAGGGCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCgg > 2:163839/1‑150 (MQ=255) gaagaaTTCGCGCTTCGACCAGGGCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCgg < 1:661551/150‑1 (MQ=255) aagaaTTCGCGCTTCGACCAGGGCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCggg < 2:133374/150‑1 (MQ=255) aagaaTTCGCGCTTCGACCAGGGCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCggg < 1:101765/150‑1 (MQ=255) aagaaTTCGCGCTTCGACCAGGGCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCggg < 1:589306/150‑1 (MQ=255) tCGCGCTTCGACCAGGGCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCAAGCCCGGGGttctg < 1:666116/150‑4 (MQ=255) gcgcTTCGACCAGGGCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAAAACCCCGGGCAAGCCCGGGGttctgt < 2:641719/149‑5 (MQ=255) gcgcTTCGACCAGGGCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAAAACCCCGGGCAAGCCCGGGGttctgt > 1:641719/1‑145 (MQ=255) gcTTCGACCAGGGCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCAAGCCCGGGGt < 2:76580/142‑1 (MQ=255) gcTTCGACCAGGGCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCAAGCCCGGGGt > 1:76580/1‑142 (MQ=255) gcTTCGACCAGGGCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGAGTGCTAAACAGAGAACCCCGGGCTTGCCCGGGGGttttgctt > 1:507295/1‑150 (MQ=255) cGACCAGGGCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTttttgcttttgc < 1:999778/150‑1 (MQ=255) ccAGGGCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCaa > 2:960318/1‑150 (MQ=255) ccAGGGCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGGTTTTGCTTTTGCCaa > 1:941200/1‑150 (MQ=255) ccAGGGCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCAGGCTTGCCCGGGGTTTTTGCTTTTGCCaa > 2:90805/1‑150 (MQ=255) cAGGGCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCaaa > 2:939213/1‑150 (MQ=255) cAGGGCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGAGAGAAAAAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCaaa > 2:1122153/1‑150 (MQ=255) gggCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGt < 2:815378/150‑1 (MQ=255) gggCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGt > 2:1067759/1‑150 (MQ=255) gtTGAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGACCCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGctacta < 2:130502/150‑1 (MQ=255) ttCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGTGGTTTTTGCTTTTGCCAAAGTGGGCTACTAg > 2:580432/1‑150 (MQ=255) tCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGAGTGCTAAACACAGATCCCCGGTCTTGTCCGGGGTTGTTGCTTTTGCCAAAGTGGGCTACGAgg < 2:710087/150‑1 (MQ=255) tCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAAGCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTGGCCAAAGTGGGCTACTAgg < 2:159980/150‑1 (MQ=255) aTGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGc < 1:465573/126‑1 (MQ=255) aTGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGc > 2:465573/1‑126 (MQ=255) aTGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGtt < 2:994967/150‑1 (MQ=255) cgcgCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCTGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATc > 2:249111/1‑150 (MQ=255) gcgcCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCt < 1:939213/150‑1 (MQ=255) cAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGaa > 2:944781/1‑150 (MQ=255) ggCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGGACCCCGGGCTTGCCCGGGGtttt > 1:521661/1‑76 (MQ=255) ggCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGGACCCCGGGCTTGCCCGGGGtttt < 2:521661/76‑1 (MQ=255) tGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGgaaga > 2:1114807/1‑150 (MQ=255) gTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGaagaag < 1:684262/150‑1 (MQ=255) tAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGt < 1:960318/150‑1 (MQ=255) gCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGa < 1:1122153/150‑1 (MQ=255) ccGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATCAGGCGCAGTGGGGTGATCTATGTCAGAGGAAGTGAACCGTGGAAGAAGTGAt > 2:68036/1‑150 (MQ=255) tCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTCCGGCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATCGaaca < 1:68036/150‑1 (MQ=255) tAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATCGAACAACTCCGTGAAGCGa > 2:286689/1‑150 (MQ=255) gaTCGTTGCGTGCTAAAGACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGGGATCGAACAACTCCGTGAAGCGaa < 2:371304/149‑1 (MQ=255) aTCGCTGCGTGCTAAACACAGAACCCCAGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATCGAACAACTCCGTGAAGCGAAt < 1:90805/150‑1 (MQ=255) cTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATCGAACAACTCCGTGAAGCGAATGAGc < 1:1114807/150‑1 (MQ=255) cTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGa < 2:711039/116‑1 (MQ=255) cTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGa > 1:711039/1‑116 (MQ=255) acaGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATCGAACAACTCCGTGAAGCGAATGAGCCGGTACCGGTGcc > 1:405373/1‑150 (MQ=255) | CTTCCGTGGCGTGTCGAAGAATTCGCGCTTCGACCAGGGCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATCGAACAACTCCGTGAAGCGAATGAGCCGGTACCGGTGCC > NC_002947/6103952‑6104244 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |