Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,170,990 G→A 7.5% T456T (ACC→ACT)  glmS ← L‑glutamine/D‑fructose‑6‑phosphate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,170,9900GA 7.5% 136.1 / 2.1 53T456T (ACC→ACT) glmSL‑glutamine/D‑fructose‑6‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (25/24);  new base A (2/2);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.94e-01

TCGCCTGCTGGGTATGCTTCGGCGTGGATGTAGGAGATTTCTTTAAGCTTGAGAGCACCTTCCATCGCCACCGGGTACTGCGCGCCGCGGCCGAGGAACAGGGTATGGTGCTTGTCGGCGAACAGCTCGGCAATTTTCTCGACGGTGGCATCCATGGCCAGGGCTTCGCCCAGGCGGGTAGGCAGTCGACGCAGCTCTTCAACCAGCTCAGCTTCGACACCGGCTTCCAGCGTACCGCGCACCTGACCAAGGGCCAGGGTCAGCAGCATCAGCGAAACCAGCTGAGTGGT  >  NC_002947/6170847‑6171136
                                                                                                                                               |                                                                                                                                                  
tCGCCTGCTGGGTATGCTTCGGCGTGGATGTAGGAGATTTCTTTAAGCTTGAGAGCACCTTCCATCGCCACCGGGTACTGCGCGCCGCGGCCGAGGAACAGGGTATGGTGCTTGTCGGCGAACAGCTCGGCAATTTTCTCGACAGTGGCa                                                                                                                                              >  2:195798/1‑150 (MQ=255)
tCGCCTGCTGGGTATGCTTCGGCGTGGATGTAGGAGATTTCTTTAAGCTTGAGAGCACCTTCCATCGCCACCGGGTACTGCGCGCCGCGGCCGAGGAACAGGGTATGGTGCTTGTCGGCGAACAGCTCGGCAATTTTCTCGACAGTGGCa                                                                                                                                              >  2:195871/1‑150 (MQ=255)
tCGCCTGCTGGGTATGCTTCGGCGTGGATGTAGGAGATTTCTTTAAGCTTGAGAGCACATTCCATCGCCACCGGGAACTGCGCGCCGCGGCCGAGGAACAGGGTATGGTGCTTGTCGGCGAACAGCTCGGCAATTTTCTCGACGGTGGCa                                                                                                                                              >  1:410959/1‑150 (MQ=255)
    ctgctgGGTATGCTTCGGCGTGGATGTAGGAGATTTCTTTAAGCTTGAGAGCACCTTCCATCGCCACCGGGTACTGCGCGCCGCGGCCGAGGAACAGGGTATGGTGCTTGTCGGCGAACAGCTCGGCAATTTTCTCGACGGTGGCATCCa                                                                                                                                          >  1:216984/1‑150 (MQ=255)
     tgctgGGTATGCTTCGGCGTGGATGTAGGAGATTTCTTTAAGCTTGAGAGCACCTTCCGTCGCCACCGGGTACTGCGCGCCGCGGCCGAGGAACAGGGTATGGTGCTTGTCGGCGAACAGCTCGGCAATTTTCTCGACGGTGGCATCCaa                                                                                                                                         <  2:17135/150‑2 (MQ=255)
       ctgGGTATGCTTCGGCGTGGATGTAGGAGATTTCTTTAAGCTTGAGAGCACCTTCCATCGCCACCGGGTACTGCGCGCCGCGGCCGAGGAACAGGGTATGGTGCTTGTCGGCGAACAGCTCGGCAATTTTCTCGACGGTGGCATCCATgg                                                                                                                                       >  2:833211/1‑150 (MQ=255)
       ctgGGTATGCTTCGGCGTGGATGTAGGAGATTTCTTTAAGCTTGAGAGCACCTTCCATCGCCACCGGGTACGGCGCGCCGCGGCCGAGGAACAGGGTATGGTGCTTGTCGGCGAACAGCTCGGCAATTTTCTCGACGGTGGCATCCATgg                                                                                                                                       <  1:1072326/150‑1 (MQ=255)
        tgGGTATGCTTCGGCGTGGATGTAGGAGATTTCTTTAAGCTTGAGAGCACCTTCCATCGCCACCGGGTACTGCGCGCCGCGGCCGAGGAACAGGGTATGGTGCTTGTCGGCGAACAGCGCGGCAATTTTCTGGACGGTGGCATCCATGGc                                                                                                                                      >  2:217939/1‑150 (MQ=255)
            tATGCTTCGGCGTGGATGTAGGAGATTTCTTTAAGCTTGAGAGCACCTTCCATCGCCACCGGGTACTGCGCGCCGCGGCCGAGGAACAGGGTATGGTGCTTGTCGGCGAACAGCTCGGCAATTTTCTCGACGGTGGCATCCATGGCCAgg                                                                                                                                  >  2:356445/1‑150 (MQ=255)
              tGCTTCGGCGTGGATGTAGGAGATTTCTTTAAGCTTGAGAGCACCTTCCATCGCCACCGGGTACTGCGCGCCGCGGCCGAGGAACAGGGTATGGTGCTTGTCGGCGAACAGCTCGGCAATTTTCTCGACGGTGGCATCCATGGCCAGGGc                                                                                                                                <  2:988680/150‑1 (MQ=255)
              tGCTTCGGCGTGGATGTAGGAGATTTCTTTAAGCTTGAGAGCACCTTCCATCGCCACCGGGTACTGCGCGCCGCGGCCGAGGAACAGGGTATGGTGCTTGTCGGCGAACAGCTCGGCAATTTTCTCGACGGTGGCATCCATGGCCAGGGc                                                                                                                                <  2:410959/150‑1 (MQ=255)
              tGCTTCGGCGTGGATGTAGGAGATTTCTTTAAGCTTGAGAGCACCTTCCATCGCCACCGGGTACTGCGCGCCGCGGCCGAGCAACCGCGTATGGTGCTTGTCGCCGAACAGCTCGGCAATTGTCTCGACGGTGGCATCCATGGCCAGGGc                                                                                                                                >  2:386246/1‑150 (MQ=255)
                         ggATGTAGGAGATTTCTTTAAGCTTGAGAGCACCTTCCATCGCCACCGGGTACTGCGCGCCGCGGCCGAGGAACAGGGTATGGTGCTTGTCGGCGAACAGCTCGGCAATTTTCTCGACGGTGGCATCCATGGCCAGGGCTTCGCCCAGGc                                                                                                                     >  1:701611/1‑150 (MQ=255)
                              tAGGAGATTTCTTTAAGCTTGAGAGCACCTTCCATCGCCACCGGGTACTGCGCGCCGCGGCCGAGGAACAGGGTATGGTGCTTGTCGGCGAACAGCTCGGCAATTTTCTCGACGGTGGCATCCATGGCCAGGGCTTCGCCCAGGCGGGTa                                                                                                                <  2:132063/150‑1 (MQ=255)
                               aGGAGATTTCTTTAAGCTTGAGAGCACCTTCCATCGCCACCGGGTACTGCGCGCCGCGGCCGAGGAACAGGGTATGGTGCTTGTCGGCGAACAGCTCGGCAATTTTCTCGACGGTGGCATCCATGGCCAGGGCTTCGCCCAGGCGGGTAg                                                                                                               <  2:505749/150‑1 (MQ=255)
                                 gagaTTTCTTTAAGCTTGAGAGCACCTTCCATCGCCACCGGGTACTGCGCGCCGCGGCCGAGGAACAGGGTATGGTGCTTGTCGGCGAACAGCTCGGCAATTTTCTCGACGGTGGCATCCATGGCCAGGGCTTCGCCCAGGCGGGTAGGc                                                                                                             >  2:186790/1‑150 (MQ=255)
                                   gaTTTCTTTAAGCTTGAGAGCACCTTCCATCGCCACCGGGTACTGCGCGCCGCGGCCGAGGAACAGGGTATGGTGCTTGTCGGCGAACAGCTCGGCAATTTTCTCGACGGTGGCATCCATGGCCAGGGCTTc                                                                                                                             <  1:67431/132‑1 (MQ=255)
                                   gaTTTCTTTAAGCTTGAGAGCACCTTCCATCGCCACCGGGTACTGCGCGCCGCGGCCGAGGAACAGGGTATGGTGCTTGTCGGCGAACAGCTCGGCAATTTTCTCGACGGTGGCATCCATGGCCAGGGCTTc                                                                                                                             >  2:67431/1‑132 (MQ=255)
                                   gaTTTCTTTAAGCTTGAGAGCACCTTCCATCGCCACCGGGTACCGCGCGCCGTGTCGGGGGAACAGGGTATGGTGCTTGTCGGCGAACAGCTCGGCAATTTTCTCGACGGTGGCATCCATGGCCAGGGCTTCGCCCAGGCGGGTAGGCAg                                                                                                           >  2:431340/1‑150 (MQ=255)
                                         tttAAGCTTGAGAGCACCTTCCATCGCCNCCGGGTACTGCGCGCNGNGGCCGAGGAACAGGGTATGGTGCTTGTCGGCGAACAGCTCGGCGATTTTCTCGACGGTGGCATTTTTAGTCAGGGCTTCGCCCAGGCGGGTAGGCAGTCNTCg                                                                                                     >  1:608337/1‑150 (MQ=255)
                                         tttAAGCTTGAGAGCACCTTCCATCGCCACCGGGTACTGCGCGCCGCGGCCGAGGAACAGGGTATGGTGCTTGTCGGCGAACAGCTCGGCAATTTTCTCGACGGTGGCATCCATGGCCAGGGCTTCGCCCAGGCGGGTAGGCAGTCGACg                                                                                                     <  1:452128/150‑1 (MQ=255)
                                         tttAAGCTTGAGAGCACCTTCCATCGCCACCGGGTACTGCGCGCCGCGGCCGAGGAACAGGGTATGGTGCTTGTCGGCGAACAGCTCGGCAATTTTCTCGACGGTGGCATCCATGGCCAGGGCTTCGCCCAGGCGGGTAGGCAGTCGACg                                                                                                     >  1:127411/1‑150 (MQ=255)
                                         tttAAGCTTGAGAGCACCTTCCATCGCCACCGGGTACTGCGCGCCGCGGCCGAGGAACAGGGTATGGTGCTTGTCGGCGAACAGCTCGGCAATTTTCTCGACGGTGGCATCCATGGCCAGGGCTTCGCCCAGGCGGGGAGGCAGTCGACg                                                                                                     >  1:1075528/1‑150 (MQ=255)
                                               cTTGAGAGCACCTTCCATCGCCACCGGGTACTGCGCGCCGCGGCCGAGGAACAGGGTATGGTGCTTGTCGGCGAACAGCTCGGCAATTTTCTCGACGGTGGCATCCATGGCCAGGGCTTCGCCCAGGCGGGTAGGCAGTCGACGCAGctc                                                                                               >  1:832697/1‑150 (MQ=255)
                                               cTTGAGAGCACCTTCCATCGCCACCGGGTACTGCGCGCCGCGGCCGAGGAACAGGGTATGGTGCTTGTCGGCGAACAGCTCGGCAATTTTCTCGACGGTGGCATCCATGGCCAGGGCTTCGCCCAGGCGGGTAGGCAGTCGACGCAGctc                                                                                               >  2:945948/1‑150 (MQ=255)
                                                 tGAGAGCACCTTCCATCGCCACCGGGTACTGCGCGCCGCGGCCGAGGAACAGGGTATGGTGCTTGTCGGCGAACAGCTCGGCAATTTTCTCGACGGTGGCATCCATGGCCAGGGCTTCGCCCAGGCGGGTAGGCAGTCGACGCAGCTCtt                                                                                             >  2:396693/1‑150 (MQ=255)
                                                  gagaGCACCTTCCATCGCCACCGGGTACTGCGCGCCGCGGCCGAGGAACAGGGTATGGTGCTTGTCGGCGAACAGCTCGGCAATTTTCTCGACGGTGGCATCCATGGCCAGGGCTTCGc                                                                                                                           <  2:404013/119‑1 (MQ=255)
                                                  gagaGCACCTTCCATCGCCACCGGGTACTGCGCGCCGCGGCCGAGGAACAGGGTATGGTGCTTGTCGGCGAACAGCTCGGCAATTTTCTCGACGGTGGCATCCATGGCCAGGGCTTCGc                                                                                                                           >  1:404013/1‑119 (MQ=255)
                                                                tCGCCACCGGGTACTGCGCGCCGCGGCCGAGGAACAGGGTATGGTGCTTGTCGGCGAACAGCTCGGCAATTTTCTCGACGGTGGCATCCATGGCCAGGGCTTCGCCCAGGCGGGTAGGCAGTCGACGCAGCTCTTCAACCAGCTCAGCtt                                                                              <  2:995359/150‑1 (MQ=255)
                                                                tCGCCACCGGGTACTGCGCGCCGCGGCCGAGGAACAGGGTATGGTGCTTGTCGGCGAACAGCTCGGCAATTTTCTCGACGGTGGCATCCATGGCCAGGGCTTCGCCCAGGCGGGTAGGCAGTCGACGCAGCTCTTCAACCAGCTCAGCtt                                                                              >  1:1071051/1‑150 (MQ=255)
                                                                 cGCTACTGGGTACTCCGCGCCGCGGCCGAGGAACAGGGTACAATACATATCGTCCGATTTTTAATCAATTTGCTCGAAGGTGGCATCCATGGCCAGGGCTTCGCCCAGGCGGGTAGGCAGTCGACGCAGCTCTTCAACCAGCTCAGCTTc                                                                             <  1:406970/150‑1 (MQ=255)
                                                                 cGCCACCGGGTACTGCGCGCCGCGGCCGAGGAACAGGGTATGGTGCTTGTCGGCGAACAGCTCGGCAATTTTCTCGACGGTGGCATCCATGGCCAGGGCTTCGCCCAGGCGGGTAGGCAGTCGACGCAGCTCTTCAACCAGCTCAGCTTc                                                                             <  2:473442/150‑1 (MQ=255)
                                                                    cACCGGGTACTGCGCGCCGCGGCCGAGGAACAGGGTATGGTGCTTGTCGGCGAACAGCTCGGCAATTTTCTCGACGGTGGCATCCATGGCCAGGGCTTCGCCCAGGCGGGTAGGCAGTCGAc                                                                                                      >  1:889232/1‑122 (MQ=255)
                                                                    cACCGGGTACTGCGCGCCGCGGCCGAGGAACAGGGTATGGTGCTTGTCGGCGAACAGCTCGGCAATTTTCTCGACGGTGGCATCCATGGCCAGGGCTTCGCCCAGGCGGGTAGGCAGTCGAc                                                                                                      <  2:889232/122‑1 (MQ=255)
                                                                          gTACTGCGCGCCGCGGCCGAGGAACAGGGTATGGTGCTTGTCGGCGAACAGCTCGGCAATTTTCTCGACGGTGGCATCCATGGCCAGGGCTTCGCCCAGGCGGGTAGGCAGTCGACGCAGCTCTTCAACCAGCTCAGCTTCGACACCGGc                                                                    <  1:1015471/150‑1 (MQ=255)
                                                                                        ggCCGAGGAACAGGGTATGGTGCTTGTCGGCGAACAGCTCGGCAATTTTCTCGACGGTGGCATCCATGGCCAGGGCTTCGCCCAGGCGGGTAGGCAGTCGACGCAGCTCTTCAACCAGCTCAGCTTCGACACCGGCTTCCAGCGTAccac                                                      >  2:872664/1‑148 (MQ=255)
                                                                                                aaCAGGGTATGGTGCCTGTAGGCGAACCGCATGTAAGATTTCTCGACGGTGGCATCCATGGCCAGGGCTTCGCCCAGGCGGGTAGGCAGTCGACGCAGCTCTCCAACCAGCTCAGCTTCGACACCGGCTTCCAGCGTACCGCGCACCTGa                                              <  2:675316/150‑1 (MQ=255)
                                                                                                          ggTGCTTGTCGGCGAACAGCTCGGCAATTTTCTCGACGGTGGCATCCATGGCCAGGGCTTCGCCCAGGCGGGTAGGCAGTCGACGCAGCTCTTCAACCAGCTCAGCTTCGACACCGGCTTCCAGCGTACCGCGCACCTGACCAAGGGCCa                                    >  1:538922/1‑150 (MQ=255)
                                                                                                           gTGCTTGTCGGCGAACAGCTCGGCAATTTTCTCGACGGTGGCATCCATGGCCAGGGCTTCGCCCAGGCGGGTAGGCAGTCGACGCAGCTCTTCAACCAGCTCAGCTTCGACACCGGCTTCCAGCGTACCGCGCACCTGACCAAGGGCCAg                                   >  1:113432/1‑150 (MQ=255)
                                                                                                            ggCTTGTCGGCGAACAGCTCGGCAATTTTCTCGACGGTGGCATCCATGGCCAGGGCTTCGCCCAGGCGGGTAGGCAGTCGACGCAGCTCTTCAACCAGCTCAGCTTCGACACCGGCTTCCAGCGTACCGCGCACCTGACCAAGGGCCAgg                                  <  1:431340/149‑1 (MQ=255)
                                                                                                                   cGGCGAACAGCTCGGCAATTTTCTCGACGGTGGCAAAATTTACCAGGGCTTCGCCCAGGCGGGTAGGCAGTCGACGCAGCTCTTCAACCAGCTCAGCTTCGACACCGGCTTCCAGCGTACCGCGCACCTGACCAAGGGCCAGGGTcagca                           <  2:820242/150‑1 (MQ=255)
                                                                                                                     gCGAACAGCTCGGCAATTTTCTCGACGGTGGCATCCATGGCCAGGGCTTCGCCCAGGCGGGTAGGCAGTCGACGCAGCTCTTCAACCAGCTCAGCTTCGACACCGGCTTCCAGCGTACCGCGCACCTGACCAAGGGCCAGGGTCagcagc                         >  2:47771/1‑150 (MQ=255)
                                                                                                                     gCGAACAGCTCGGCAATTTTCTCGACAGTGGCATCCATGGCCAGGGCTTCGCCCAGGCGGGTAGGCAGTCGACGCAGCTCTTCAACCAGCTCAGCTTCGACACCGGCTTCCAGCGTACCGCGCACCTGACCAAGGGCCAGGGTCagcagc                         <  1:195871/150‑1 (MQ=255)
                                                                                                                     gCGAACAGCTCGGCAATTTTCTCGACAGTGGCATCCATGGCCAGGGCTTCGCCCAGGCGGGTAGGCAGTCGACGCAGCTCTTCAACCAGCTCAGCTTCGACACCGGCTTCCAGCGTACCGCGCACCTGACCAAGGGCCAGGGTCagcagc                         <  1:195798/150‑1 (MQ=255)
                                                                                                                        aaCAGCTCGGCAATTTTCTCGACGGTGGCATCCATGGCCAGGGCTTCGCCCAGGCGGGTAGGCAGTCGACGCAGCTCTTCAACCAGCTCAGCTTCGACACCGGCTTCCAGCGTACCGCACACCTGACCAAGGGCCAGGGTCAGCAGCATc                      >  2:983301/1‑150 (MQ=255)
                                                                                                                           aGCTCGGCAATTTTCTCGACGGTGGCATCCATGGCCAGGGCTTCGCCCAGGCGGGTGGGCAGTGGACGCAGCTCTTCAACCAGCTCAGCTTCGACACCGGCTTCCAGCGTACCGCGCACCTGACCAAGGGCCAGGGTCAGCAGCATCAGc                   >  2:799322/1‑150 (MQ=255)
                                                                                                                           aGCTCGGCAATTTTCTCGACGGTGGCATCCATGGCCAGGGCTTCGCCCAGGCGGGTAGGCAGTCGACGCAGCTCTTCAACCAGCTCAGCTTCGACACCGGCTTCCAGCGTACCGCGCACCTGACCAAGGGCCAGGGTCAGCAGCATCAGc                   <  1:396693/150‑1 (MQ=255)
                                                                                                                           aGCTCGGCAATTTTCTCGACGGTGGCATCCATGGCCAGGGCTTCGCCCAGGCGGGTAGGCAGTCGACGCAGCTCTTCAACCAGCTCAGCTTCGACACCGGCTTCCAGCGTACCGCGCACCTGACCAAGGGCCAGGGTCAGCAGCATCAGc                   <  2:1075528/150‑1 (MQ=255)
                                                                                                                               cGGCAATTTTCTCGACGGTGGCATCCATGGCCAGGGCTTCGCCCAGGCGGGTAGGCAGTCGACGCAGCTCTTCAACCAGCTCAGCTTCGACACCGGCTTCCAGCGTACCACGCACCTGACCAAGGGCCAGGGTCAGCAGCATCAGCGaaa               <  1:872664/150‑1 (MQ=255)
                                                                                                                                     ttttCTCGACGGTGGCATCCATGGCCAGGGCTTCGCCCAGGCGGGTAGGCAGTCGACGCAGCTCTTCAACCAGCTCAGCTTCGACACCGGCTTCCAGCGTACCGCGCACCTGACCAAGGGCCAGGGTCAGCAGCATCAGCGAAACCAGCt         <  2:1071051/150‑1 (MQ=255)
                                                                                                                                          tcGACGGTGGCATCCATGGCCAGGGCTTCGCCCAGGCGGGTAGGCAGTCGACGCAGCTCTTCAACCAGCTCAGCTTCGACACCGGCTTCCAGCGTACCGCGCACCTGACCAAGGGCCAGTGTCAGCAGCATCAGCGAAACCAGCTGAgtg    <  2:127411/150‑1 (MQ=255)
                                                                                                                                            gACGGTGGCATCCATGGCCAGGGCTTCGCCCAGGCGGGTAGGCAGTCGACGCAGCTCTTCAACCAGCTCAGCTTCGACACCGGCTTCCAGCGTACCGCGCACCTGACCAAGGGCCAGGGTCAGCAGCATCAGCGAAACCAGCTGAgnggt  <  2:701611/150‑1 (MQ=255)
                                                                                                                                            gACGGTGGCATCCATGGCCAGGGCTTCGCCCAGGCGGGTAGGCAGTCGACGCAGCTCTTCAACCAGCTCAGCTTCGACACCGGCTTCCAGCGTACCGCACACCTGACCAAGGGCCAGGGTCAGCAGCATCAGCGAAACCAGCTGAgtggt  <  1:983301/150‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
TCGCCTGCTGGGTATGCTTCGGCGTGGATGTAGGAGATTTCTTTAAGCTTGAGAGCACCTTCCATCGCCACCGGGTACTGCGCGCCGCGGCCGAGGAACAGGGTATGGTGCTTGTCGGCGAACAGCTCGGCAATTTTCTCGACGGTGGCATCCATGGCCAGGGCTTCGCCCAGGCGGGTAGGCAGTCGACGCAGCTCTTCAACCAGCTCAGCTTCGACACCGGCTTCCAGCGTACCGCGCACCTGACCAAGGGCCAGGGTCAGCAGCATCAGCGAAACCAGCTGAGTGGT  >  NC_002947/6170847‑6171136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑