Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,171,410 C→A 8.7% L316L (CTG→CTT)  glmS ← L‑glutamine/D‑fructose‑6‑phosphate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,171,4100CA 8.7% 130.6 / 3.7 46L316L (CTG→CTT) glmSL‑glutamine/D‑fructose‑6‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base C (19/23);  new base A (2/2);  total (21/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.97e-01

CCTTGGCGTTGCGCAGTGCGGCCAAGGTATCGGCGGTTTCGCCGGACTGCGAGATGGAGACGAACAGAGTGTCCGGCTGCACCACCACCTTGCGGTAACGGAACTCGCTGGCCACTTCTACCTGGCAAGGGATGCCGGCCAGGCTTTCCAGCCAGTAGCGCGCAACCATACCGGCGTGGTAGCTGGTGCCACAGGCAACGATCTGCACGTTGCGCACCTTGGCAAACAGGTCGGCAGCTTGCGGGCCAAAGGCCTGGACCAGCACGTTGTCCTTGCCCAGGCGGCCTTCAAGCGTGCG  >  NC_002947/6171262‑6171559
                                                                                                                                                    |                                                                                                                                                     
ccTTGGCGTTGCGCAGTGCGGCCAAGGTATCGGCGGTTTCGCCGGACTGCGAGATGGAGACGAACAGAGTGTCCGGCTGCACCACCACCTTGCGGTAACGGAACTCGCTGGCCACTTCTACCTGGCAAGGGATGCCGGCCAGGCTTTcca                                                                                                                                                      <  1:117977/150‑1 (MQ=255)
     gCGTTGCGCAGTGCGGCCAAGGTATCGGCGGTTTCGCCGGACTGCGAGATGGAGACGAACAGGGTGTCCGGCTGCACCACCACCTTGCGGTAACGGAACTCGCTGGCCACTTCTACCTGGCAAGGGATGCCGGCCAGGCTTTccagccag                                                                                                                                                 <  2:301588/150‑1 (MQ=255)
     gCGTTGCGCAGTGCGGCCAAGGTATCGGCGGTTTCGCCGGACTGCGAGATGGAGACGAACAGAGTGTCCGGCTGCACCACCACCTTGCGGTAACGGAACTCGCTGGCCACTTCTACCTGGCAAGGGATGCCGGCCAGGCTTTccagccag                                                                                                                                                 <  2:749200/150‑1 (MQ=255)
       gTTGCGCAGTGCGGCCAAGGTATCGGCGGTTTCGCCGGACTGCGAGATGGAGACGAACAGAGTGTCCGGCTGCACCACCACCTTGCGGTAACGGAACTCGCTGGCCACTTCTACCTGGCAAGGGATGCCGGCCAGGCTTTCCAGCCAGTa                                                                                                                                               <  1:791006/150‑1 (MQ=255)
           cgcAGTGCGGCCAAGGTATCGGCGGTTTCGCCGGACTGCGAGATGGAGACGAACAGAGTGTCCGGCTGCACCACCACCTTGCGGTAACGGAACTCGCTGGCCACTTCTACCTGGCAAGGGATGCCGGCCAGGCTTTCCAGCCAGTAgcgc                                                                                                                                           <  1:701914/150‑1 (MQ=255)
           cgcAGTGCGGCCAAGGTATCGGCGGTTTCGCCGGACTGCGAGATGGAGACGAACAGAGTGTCCGGCTGCACCACCACCTTGCGGTAACGGAACTCGCTGGCCACTTCTACCTGGCAAGGATCGGTGGCCAGGCTTTCCAGCCAGTAgcgc                                                                                                                                           <  1:759132/150‑1 (MQ=255)
            gcAGTGCGGCCAAGGTATCGGCGGTTTCGCCGGACTGCGATATGGAGACGAACAGAGTGTCCGGCTGCACCACCACCTTGCGGTAACGGAACTCGCTGGCCACTTCTACCTGGCAAGGGATGCCGGCCAGGCTTTCCAGCCAGTAgcgcg                                                                                                                                          >  2:142824/1‑150 (MQ=255)
               gTGCGGCCAAGGTATCGGCGGTTTCGCCGGACTGCGAGATGGAGACGAACAGAGTGTCCGGCTGCACCACCACCTTGCGGTAACGGAACTCGCTGGCCACTTCTACCTGGCAAGGGATGCCGGCCAGGCTTTCCAGCCAGTAGCGCGCaa                                                                                                                                       <  2:404174/150‑1 (MQ=255)
                    gCCAAGGTATCGGCGGTTTCGCCGGACTGCGAGATGGAGACGAACAGAGTGCGCCGCTGCACCACCACCTTGCGGTAACGGAACTCGCTGGCCACTTCTACCTGGCAAGGGATGCCGGCCAGGCTTTCCAGCCAGTAGCGCGCAACCATa                                                                                                                                  >  1:631031/1‑150 (MQ=255)
                             tCGGCGGTTTCGCCGGACTGCGAGATGGAGACGAACAGAGTGTCCGGCTGCACCACCACCTTGCGGTAACGGAACTCGCTGGCCACTTCTACCTGGCAAGGGATGCCGGCCAGGCTTTCAAGCCAGTAGCGCGCAACCATACCGGCGTgg                                                                                                                         >  2:660145/1‑150 (MQ=255)
                             tCGGCGGTTTCGCCGGACTGCGAGATGGAGACGAACAGAGTGTCCGGCTGCACCACCACCTTGCGGTAACGGAACTCGCTGGCCACTTCTACCTGGCAAGGGATGCCGGCCAGGCTTTCAAGCCAGTAGCGCGCAACCATACCGGCGTgg                                                                                                                         >  2:660139/1‑150 (MQ=255)
                                  ggTTTCGCCGGACTGCGAGATGGAGACGAACAGGGTGTCCGGCTGCACCACCACCTTGCGGTAACGGAACTCGCTGGCAACTTCTACCTGGCAAGGGATGCCGTCCAGGCTTTCCAGCCAGTAGCGCGCAACCATACCGGCGTGGTAGCt                                                                                                                    <  1:862284/150‑1 (MQ=255)
                                   gTTTCGCCGGACTGCGAGATGGAGACGAACAGAGTGTCCGGCTGCACCACCACCTTGCGGTAACGGAACTCGCTGGCCACTTCTACCTGGCAAGGGATGCCGGCCAGGCTTTCCAGCCAGTAg                                                                                                                                              <  2:926336/123‑1 (MQ=255)
                                   gTTTCGCCGGACTGCGAGATGGAGACGAACAGAGTGTCCGGCTGCACCACCACCTTGCGGTAACGGAACTCGCTGGCCACTTCTACCTGGCAAGGGATGCCGGCCAGGCTTTCCAGCCAGTAg                                                                                                                                              >  1:926336/1‑123 (MQ=255)
                                     ttCGCCGGACTGCGAGATGGAGACGAACAGAGTGTCCGGCTGCACCACCACCTTGCGGTAACGGAACTCGCTGGCCACTTCTACCTGGCAAGGGATGCCGGCCAGGCTTTCCAGCCAGTAGCGCGCAACCATACCGGCGTGGTAGCTGGt                                                                                                                 <  1:1020399/150‑1 (MQ=255)
                                     ttCGCCGGACTGCGAGATGGAGACGAACAGAGTGTCCGGCTGCACCACCACCTTGCGGTAACGGAACTCGCTGGCCACTTCTACCTGGCAAGGGATGCCGGCCAGGCTTTCCAGCCAGTAGCGCGCAACCATACCGGCGTGGTAGCTGGt                                                                                                                 <  2:646646/150‑1 (MQ=255)
                                             aCTGCGAGATGGAGACGAACAGAGTGTCCGGCTGCACCACCACCTTGCGGTAACGGAACTCGCTGGCCACTTCTACCTGGCAAGGGATGCCGGCCAGGCTTTCCAGCCAGTAGCGCGCAACCATACCGGCGTGGTAGCTGGTGCCACAgg                                                                                                         >  1:611393/1‑150 (MQ=255)
                                                   agaTGGAGACGAACAGAGTGTCCGGCTGCACCACCACCTTGCGGTAACGGAACTCGCTGGCCACTTCTACCTGGCAAGGGATGCCGGCCAGGCTTTCCAGCCAGTAGCGCGCAACCATACCGGCGTGGTAGCTGGTGCCACAGGCAACGa                                                                                                   >  2:370895/1‑150 (MQ=255)
                                                    gaTGGAGACGAACAGAGTGTCCGGCTGCACCACCACCTTGCGGTAACGGAACTCGCTGGCCACTTCTACCTGGCAAGGGATGCCGGCCAGGCTTTCCAGCCAGTAGCGCGCAACCATACCGGCGTGGTAGCTGGTGCCACAGGCAACGAt                                                                                                  <  1:1012875/150‑1 (MQ=255)
                                                    gaTGGAGACGAACAGAGTGTCCGGCTGCACCACCACCTTGCGGTAACGGAACTCGCTGGCCACTTCTACCTGGCAAGGGATGCCGGCCAGGCTTTCCAGCCAGTAGCGCGCAACCATACCGGCGTGGTAGCTGGTGCCACAGGCAACGAt                                                                                                  <  1:124931/150‑1 (MQ=255)
                                                      tGGAGACGAACAGAGTGTCCGGCTGCACCACCACCTTGCGGTAACGGAACTCGCTGGCCACTTCTACCTGGCAAGGGATGCCGGTCAGGCTTTCCAGCCAGTAGCGCGCAACCATACCGGCGTGGTAGCTGGTGCCACAGGCAACGATCt                                                                                                >  1:408363/1‑150 (MQ=255)
                                                          gaCGAACAGAGTGTCCGGCTGCACCACCACCTTGCGGTAACGGAACTCGCTGGCCACTTCTACCTGGCAAGGGATGCCGGCCAGGCTTTCCAGCCAGTAGCGCGCAACCATACCAGCGTGGTAGCTGGTGCCACAGGCAACGATCTGCAc                                                                                            >  2:783526/1‑150 (MQ=255)
                                                           aCGAACAGAGTGTCCGGCTGCACCACCACCTTGCGGTAACGGAACTCGCTGGCCACTTCTACCTGGCAAGGGATGCCGGCCAGGCTTTCCAGCCAGTAGCGCGCAACCATACCGGCGTGGTAGCTGGTGCCACAGGCAACGATCTGCACg                                                                                           >  2:22418/1‑150 (MQ=255)
                                                            cGAACAGAGTGTCCGGCTGCACCACCACCTTGCGGTAACGGAACTCGCTGGCCACTTCTACCTGGCAAGGGATGCCGGCCAGGCTTTCCAGCCAGTAGCGCGCAACCATACCGGCGTGGTAGCTGGTGCCACAGGCAACGATCTGCACGt                                                                                          <  2:611393/150‑1 (MQ=255)
                                                             gAACAGAGTGTCCGGCTGCACCACCACCTTGCGGTAACGGAACTCGCTGGCCACTTCTACCTGGCAAGGGATGCCGGCCAGGCTTTCCAGCCAGTAGCGCGCAACCATACCGGCGTGGTAGCTGGTGCCACAGGCAACGATCTGCACGtt                                                                                         <  1:22418/150‑1 (MQ=255)
                                                             gAACAGAGTGTCCGGCTGCACCACCACCTTGCGGTAACGGAACTCGCTGGCCACTTCTACCTGGCAAGGGATGCCGGCCAGGCTTTCCAGCCAGTAGCGCGCAACCATACCGGCGTGGTAGCTGGTGCCACAGGCAACGATCTGCACGtt                                                                                         >  1:381239/1‑150 (MQ=255)
                                                                cAGAGTGTCCGGCTGCACCACCACCTTGCGGTAACGGAACTCGCTGGCCACTTCTACCTGGCAAGGGATGCCGGCCAGGCTTTCCAGCCAGTAGCGCGCAACCATACCGGCGTGGTAGCTGGTGCCACAGGCAACGATCTGCACGTTgcg                                                                                      >  2:400320/1‑150 (MQ=255)
                                                                       tCCGGCTGCACCACCACCTTGCGGTAACGGAACTCGCTGGCCACTTCTACCTGGCAAGGGATGCCGGCCAGGCTTTccagc                                                                                                                                                    >  2:552445/1‑81 (MQ=255)
                                                                       tCCGGCTGCACCACCACCTTGCGGTAACGGAACTCGCTGGCCACTTCTACCTGGCAAGGGATGCCGGCCAGGCTTTccagc                                                                                                                                                    <  1:552445/81‑1 (MQ=255)
                                                                       tCCGGCGGCACCACCACCTTGCGGTAACGGAACTCGCTGGCCACTTCTACCTGGCAAGGGATGCCGGCCAGGCTTTCCAGCCAGTAGCGCGCAACAATCCCGGCGTGGTAGCTGGTGCCACAGGCAACGATCTGCACGTTGCGCACCTTg                                                                               <  1:28979/150‑1 (MQ=255)
                                                                            cTGCTCCACCACCTTGCGGTAACGGAACTCGCTGGCCACTTCTACCTGGCAAGGGATGCCGGCCAGGCTTTCCAGCTAGTAGCGCGCAACCATACCGGCGTGGTAGCTGGTGCCACAGGCAACGATCTGCACGTTGCGCACCTTGGCaaa                                                                          <  2:551795/150‑1 (MQ=255)
                                                                              gCACCACCACCTTGCGGTAACGGAACTCGCTGGCCACTTCTACCTGGCAAGGGATGCCGGCCAGGCTTTCCAGCCAGTAGCGCGCAACCATACCGGCGTGGTAGCTGGTGCCACAGGCAACGATCTGCACGTTGCGCACCTTGGCAAACa                                                                        <  1:370895/150‑1 (MQ=255)
                                                                                accaccaccTTGCGGTAACGGAACTCGCTGGCCACTTCTACCTGGCAAGGGATGCCGGCCAGGCTTTccagc                                                                                                                                                    >  2:962330/1‑72 (MQ=255)
                                                                                accaccaccTTGCGGTAACGGAACTCGCTGGCCACTTCTACCTGGCAAGGGATGCCGGCCAGGCTTTccagc                                                                                                                                                    <  1:962330/72‑1 (MQ=255)
                                                                                   accaccTTGCGGTAACGGAACTCGCTGGCCACTTCTACCTGGCAAGGGATGCCGGCCAGGCTTTCCAGCCAGTAGCGCGCAACCATACCGGCGTGGTAGCTGGTGCCACAGGCAACGATCt                                                                                                <  1:483509/121‑1 (MQ=255)
                                                                                   accaccTTGCGGTAACGGAACTCGCTGGCCACTTCTACCTGGCAAGGGATGCCGGCCAGGCTTTCCAGCCAGTAGCGCGCAACCATACCGGCGTGGTAGCTGGTGCCACAGGCAACGATCt                                                                                                >  2:483509/1‑121 (MQ=255)
                                                                                        cTTGCGGTAACGGAACTCGCTGGCCACTTCTACCTGGCAAGGGATGCCGGCCAGGCTTTCCAGCCAGTAGCGCGCAACCATACCGGCGTGGTAGCTGGTGCCACAGGCAACGATCTGCACGTTg                                                                                        >  2:240328/1‑124 (MQ=255)
                                                                                        cTTGCGGTAACGGAACTCGCTGGCCACTTCTACCTGGCAAGGGATGCCGGCCAGGCTTTCCAGCCAGTAGCGCGCAACCATACCGGCGTGGTAGCTGGTGCCACAGGCAACGATCTGCACGTTg                                                                                        <  1:240328/124‑1 (MQ=255)
                                                                                                       ctcgtgggCCACTGCTACCTGGCAAGGGATGCCGGCCAGGCTTTCCAGCCAGTAGCGCGCAACCATACCGGCGTGGTAGCTGGTGCCACAGGCAACGATCTGCACGTTGCGCACCTTGGCAAACAGGTCGGCAGCTTGCGGGCCAAAGGc                                               <  2:631031/144‑1 (MQ=255)
                                                                                                       cTCGCTGGCCACTTCTACCTGGCAAGGGATGCCGGCCAGGCTTTCCAGCCAGTAGCGCGCAACCATACCGGCGTGGTAGCTGGTGCCACAGGCAACGATCTGCACGTTGCGCACCTTGGCAAACAGGTCGGCAGCTTGCGGGCCAAAGGc                                               <  1:142824/150‑1 (MQ=255)
                                                                                                            tGGCCACTTCTACCTGGCAAGGGATGCCGGCCAGGCTTTCCAGCCAGTAGCGCGCAACCATACCGGCGTGGTAGCTGGCGCCACAGGCAACGATCTGCACGTTGCGAACCTTGGCAACCAGGTCGGCAGCTTGCGGGTTAAAGGCCTTGa                                          <  2:618939/150‑1 (MQ=255)
                                                                                                              gCCACTTCTACCTGGCAAGGGATGCCGGCCAGGCTTTCCAGCCAGTAGCGCGCAACCATACCGGCGTGGTAGCTGGTGCCACAGGCAACGATCTGCACGTTGCGCACCTTGGCAAACAGGTCGGCAGCTTGCGGGCCAAAGGCCTGGAcc                                        >  2:278019/1‑150 (MQ=255)
                                                                                                               ccACTTCTACCTGGCAAGGGATGCCGGCCAGGCTTTCCAGCCAGTAGCGCGCAACCATACCGGCGTGGTAGCTGGTGCCACAGGCAACGATCTGCACGTTGCGCACCTTGGCAAACAGGTCGGCAGCTTGCGGGCCAAAGGCCTGGACCa                                       >  1:59277/1‑150 (MQ=255)
                                                                                                                cACTTCTACCTGGCAAGGGATGCCGGCCAGGCTTTCCAGCCAGTAGCGCGCAACCATACCGGCGTGGTAGCTGGTGCCACAGGCAACGATCTGCACGTTGCGCACCTTGGCAAACAGGTCGGCAGCTTGCGGGCCAAAGGCCTGGACCAg                                      >  1:634300/1‑150 (MQ=255)
                                                                                                                      tACCTGGCAAGGGATGCCGGCCAGGCTTTCCAGCCAGTAGCGCGCAACCATACCGGCGTGGTAGCTGGTGCCACAGGCAACGATCTGCACGTTGCGCACCTTGGCAAACAGGTCGGCAGCTTGCGGGCCAAAGGCCTGGACCAGCACGtt                                >  1:834204/1‑150 (MQ=255)
                                                                                                                       aCCTGGCAAGGGATGCCGGCCAGGCTTTCCAGCCAGTAGCGCGCAACCATACCGGCGTGGTAGCTGGTGCCACAGGCAACGATCTGCACGTTGCGCACCTTGGCAAACAGGTCGGCAGCTTGCGGGCCAAAGGCCTg                                            >  2:533036/1‑137 (MQ=255)
                                                                                                                       aCCTGGCAAGGGATGCCGGCCAGGCTTTCCAGCCAGTAGCGCGCAACCATACCGGCGTGGTAGCTGGTGCCACAGGCAACGATCTGCACGTTGCGCACCTTGGCAAACAGGTCGGCAGCTTGCGGGCCAAAGGCCTg                                            <  1:533036/137‑1 (MQ=255)
                                                                                                                            gCAAGGGATGCCGGCCAGGCTTTCCAGCCAGTAGCGCGCAACCATACCGGCGTGGTAGCTGGTGCCACAGGCAACGATCTGCACGTTGCGCACCTTGGCAAACAGGTCGGCAGCTTGCGGGCCAAAGGCCTGGACCAGCACGTTGTCCtt                          >  1:735666/1‑150 (MQ=255)
                                                                                                                            gCAAGGGATGCAGTCCAGGCTTTCCAGCCAGTAGCGCGCAACCATCCCGGCGTGGTAGCTGGTGCCACAGGAAACGATCTGCACGTTGCGCACCTTGGCAAACAGGTCGGCAGCTTGCGGGCCAAAGGCCTGGACCAGCACGTTGTCCtt                          <  1:783526/150‑1 (MQ=255)
                                                                                                                                 ggATGCCGGCCAGTCTTTCCAGGCAGTAGCGCGCAACCATACCTGCGTGGTAGCTGGTGCCACAGGCAACGATCTGCACGTTGCGCACCTTGGCAAACAGGTCGGCAGCTTGCGGGCCAAAGGCCTGGACCAGCACGTTGTCCTTGCCCa                     <  1:241998/150‑1 (MQ=255)
                                                                                                                                       cGGCCAGGCTTTCCAGCCAGTAGCGCGCAACCATACCGGCGTGGTAGCTGGTGCCACAGGCAACGATCTGCACGTTGCGCACCTTGGCAAACAGGTCGGCAGCTTGCGGGCCAAAGGCCTGGATTATGACGTTGTCCTTGCCCAggcggc               >  1:362/1‑150 (MQ=255)
                                                                                                                                               cTTTCAAGCCAGTAGCGCGCAACCATACCGGCGTGGTAGCTGGTGCCACAGGCAACGATCTGCACGTTGCGCACCTTGGCAAACAGGTCGGCAGCTTGCGGGCCAAAGGCCTGGACCAGCACGTTGTCCTTGCCCAGGCGGCCTTCAAGc       <  1:660139/150‑1 (MQ=255)
                                                                                                                                               cTTTCAAGCCAGTAGCGCGCAACCATACCGGCGTGGTAGCTGGTGCCACAGGCAACGATCTGCACGTTGCGCACCTTGGCAAACAGGTCGGCAGCTTGCGGGCCAAAGGCCTGGACCAGCACGTTGTCCTTGCCCAGGCGGCCTTCAAGc       <  1:660145/150‑1 (MQ=255)
                                                                                                                                                   ccagccagTAGCGCGCAACCATACCGGCGTGGTAGCTGGTGCCACAGGCAACGATCTGCACGTTGCGCACCTTGGCAAACAGGTCGGCAGCTTGCGGGCCAAAGGCCTGGACCAGCACGTTGTCCTTGCCCAGGCGGCCTTCAAGCGTgc   <  2:634300/150‑1 (MQ=255)
                                                                                                                                                   ccagccagTAGCGCGCAACCATACCGGCGTGGTAGCTGGTGCCACAGGCAACGATCTGCACGTTGCGCACCTTGGCAAACAGGTCGGCAGCTTGCGGGCCAAAGGCCTGGACCAGCACGTTGTCCTTGCCCAGGCGGCCTTCAAGCGTgc   >  1:309523/1‑150 (MQ=255)
                                                                                                                                                    cagccagTAGCGCGCAACCATACCGGCGTGGTAGCTGGTGCCACAGGCAACGATCTGCACGTTGCGCACCTTGACAAACAGGTCGGCAGCTTGCGGGCCAAAGGCCTGGACCAGCACGTTGTCCTTGCCCAGGCGGCCTTCAAGCGTgcg  >  1:541006/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CCTTGGCGTTGCGCAGTGCGGCCAAGGTATCGGCGGTTTCGCCGGACTGCGAGATGGAGACGAACAGAGTGTCCGGCTGCACCACCACCTTGCGGTAACGGAACTCGCTGGCCACTTCTACCTGGCAAGGGATGCCGGCCAGGCTTTCCAGCCAGTAGCGCGCAACCATACCGGCGTGGTAGCTGGTGCCACAGGCAACGATCTGCACGTTGCGCACCTTGGCAAACAGGTCGGCAGCTTGCGGGCCAAAGGCCTGGACCAGCACGTTGTCCTTGCCCAGGCGGCCTTCAAGCGTGCG  >  NC_002947/6171262‑6171559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑