Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	6,180,715	A→T	I263N (ATC→AAC)	atpB ←	ATP synthase subunit a

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,180,715	0	A	T	100.0%	144.5 / NA	44	I263N (ATC→AAC)	atpB	ATP synthase subunit a
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (21/23);  total (21/23)

CTTGCGGACTTTTTAAGCCCTCCCCCCGTACAGGCAGGGAAGGCTATCAGACACGCCAGGCG‑GTCTTAATGGTTATCTTCGTGAGCCATCGACAGGTAGACGATGGTCAGCATCATGAAGATGAAAGCTTGCAGGGTGATGATCAGGATGTGGAACACAGCCCACGCCCATTGCAGCACCACACCCAGGCCGCTGAGCCACAGCAGGCCGGAGCCGAACATTACAGCGATCAGGATGAACACCAGCTCGCCAGCATACATGTTGCCGAACAGACGCAGTGCCAGCGAGAT  >  NC_002947/6180568‑6180857                                                                                                                                                     |                                                                                                                                               cTTGCGGACTTTTTAAGCCCTCCCCCCGTACAGGCAGGGAAGGCTATCAGACACGCCAGGCG‑GTCTTAATGGTTATCTTCGTGAGCCATCGACAGGTAGACGATGGTCAGCATCATGAAGATGAAAGCTTGCAGGGTGATGATCAGGTtg                                                                                                                                              >  1:46411/1‑150 (MQ=255)  ttGCGGACTTTTTAAGCCCTCCCCCCGTACAGGCAGGGAAGGCTATCAGACACGCCAGGCG‑GTCTTAATGGTTATCTTCGTGAGCCATCGACAGGTAGACGATGGTCAGCATCATGAAGATGAAAGCTTGCAGGGTGATGATCAGGTtgt                                                                                                                                             >  2:343706/1‑150 (MQ=255)           ttttAAGCCCTCCCCCCGTACAGGCAGGGAAGGCTATCAGACACGCCAGGCG‑GTCTTAATGGTTATCTTCGTGAGCCATCGACAGGTAGACGATGGTCAGCATCATGAAGATGAAAGCTTGCAGGGTGATGATCAGGTTGTGGAACACAg                                                                                                                                    <  1:569934/150‑1 (MQ=255)           ttttAAGCCCTCCCCCCGTACAGGCAGGGAAGGCTATCAGACACGCCAAGCG‑GTCTTAATGGTTATCTTCGTGCAAAATCAAACGGTACCGGGAGAGCAGGAGCAGGAAGATTAAAGCTTGCAGGGTGATGATCAGGTTGTGGAACACAg                                                                                                                                    <  1:343706/150‑1 (MQ=255)                  cccTCCCCCCGTACAGGCAGGGAAGGCTATCAGACACGCCAGGCG‑GTCTTAATGGTTATCTTCGTGAGCCATCGACAGGTAGGCGATGGTCAGCATCATGAATATGAAAGCTTGCAGGGTGATGATCAGGTTGTGGAACACAGCCCACGc                                                                                                                             >  1:592033/1‑150 (MQ=255)                       cccccGTACAGGCAGGGAAGGCTATCAGACACGCCAGGCG‑GTCTTAATGGTTATCTTCGTGAGCCATCGACAGGTAGACGATGGTCAGCATCATGAAGATGAAAGCTTGCAGGGTGATGATCAGGTTGTGGAACACAGCCCACGCCCAtt                                                                                                                        >  1:761666/1‑150 (MQ=255)                        ccccGTACAGGCAGGGAAGGCTATCAGACACGCCAGGCG‑GTCTTAATGGTTATCTTCGTGAGCCATCGACAGGTAGACGATGGTCAGCATCATGAAGATGAAAGCTTGCAGGGTGATGATCAGGTTGTGGAACACAGCCCACGCCCATTg                                                                                                                       >  1:1136680/1‑150 (MQ=255)                            gTACAGGCAGGGAAGGCTATCAGACACGCCAGGCG‑GTCTTAATGGTTATCTTCGTGAGCCATCGACAGGTAGACGATGGTCAGCATCATGAAGATGAAAGCTTGCAGGGTGATGATCAGGTTGTGGAACACAGCCCACGCCCATTgcagc                                                                                                                   <  1:918496/150‑1 (MQ=255)                            gTACAGGCAGGGAAGGCTATCAGACACGCCAGGCG‑GTCTTAATGGTTATCTTCGTGAGCCATCGACAGGTAGACGATGGTCAGCATCATGAAGATGAAAGCTTGCAGGGTGATGATCAGGTTGTGGAACACAGCCCACGCCCATTgcagc                                                                                                                   <  1:5190/150‑1 (MQ=255)                             tACAGGCAGGGAAGGCTATCAGACACGCCAGGCG‑GTCTTAATGGTTATCTTCGTGAGCCATCGACAGGTAGACGATGGTCAGCATCATGAAGATGAAAGCTTGCAGGGTGATGATCAGGTTGTGGAACACAGCCCACGCCCATTgcagca                                                                                                                  <  2:1041788/150‑1 (MQ=255)                                   caggGAAGGCTATCAGACACGCCAGGCG‑GTCTTAATGGTTATCTTCGTGAGCCATCGACAGGTAGACGATGGTCAGCATCATGAAGATGAAAGCTTGCAGGGTGATGATCAGGTTGTGGAACACAGCCCACGCCCATTGCAGCACCacac                                                                                                            <  1:783144/150‑1 (MQ=255)                                       gAAGGCTATCAGACACGCCAGGCG‑GTCTTAATGGTTATCTTCGTGAGCCATCGACAGGTAGACGATGGTCAGCATCATGAAGATGAAAGCTTGCAGGGTGATGATCAGGTTGTGGAACACAGCCCACGCCCATTGCAGCACCACACCCAg                                                                                                        <  1:358021/150‑1 (MQ=255)                                        aaGGCTATCAGACACGCCAGGCG‑GTCTTAATGGTTATCTTCGTGAGCCATCGACAGGTAGACGATGGTCAGCATCATGAAGATGAAAGCTTGCAGGGTGATGATCAGGTTGTGGAACACAGCCCACGCCCATTGCAGCACCACACCCAgg                                                                                                       <  1:1218540/150‑1 (MQ=255)                                                taagaccGCCTGGCGTGTCTTAATGGTTATCTTCGTGAGCCATCGACAGGTAGACGATGGTCAGCATCATGAAGATGAAAGCTTGCAGGGTGATGATCAGGTTGTGGAACACAGCCCACGCCCATTGCAGCACCACACCCAGGCCGCTGa                                                                                                <  1:638497/144‑1 (MQ=255)                                                      cGCCAGGCG‑GTCTTAATGGTTATCTTCGTGAGCCATCGACAGGTAGACGATGGTCAGCATCATGAAGATGAAAGCTTGCAGGGTGATGATCAGGTTGTGGAACACAGCCCACGCCCATTGCAGCACCACACCCAGGCCGCTGAGCCAcag                                                                                         <  1:1091223/150‑1 (MQ=255)                                                         cAGGCG‑GTCTTAATGGTTATCTTCGTGAGCCATCGACAGGTAGACGATGGTCAGCATCATGAAGATGAAAGCTTGCAGGGTGATGATCAGGTTGTGGAACACAGCCCACGCCCATTGCAGCACAACACCCTGACCGCTGAGCCAcagcag                                                                                      >  2:294055/1‑150 (MQ=255)                                                           ggCG‑GTCTTAATGGTTATCTTCGTGAGCCATCGACAGGTAGACGATGGTCAGCATCATGAAGATGAAAGCTTGCAGGGTGATGATCAGGTTGTGGAACACAGCCCACGCCCATTGCAGCACCACACCCAGGCCGCTGa                                                                                                >  1:528236/1‑138 (MQ=255)                                                           ggCG‑GTCTTAATGGTTATCTTCGTGAGCCATCGACAGGTAGACGATGGTCAGCATCATGAAGATGAAAGCTTGCAGGGTGATGATCAGGTTGTGGAACACAGCCCACGCCCATTGCAGCACCACACCCAGGCCGCTGa                                                                                                <  2:528236/138‑1 (MQ=255)                                                                gTCTTAATGGTTATCTTCGTGAGCCATCGACAGGTAGACGATGGTCAGCATCATGAAGATGAAAGCTTGCAGGGTGATGATCAGGTTGTGGAACACAGCCCACGCCCATTGCAGCACCACACCCAGGCCGCTGAGCCACAGCAGGCCGGa                                                                                <  2:46411/150‑1 (MQ=255)                                                                         gTTATCTTCGTGAGCCATCGACAGGTAGACGATGGTCAGCATCATGAAGATGAAAGCTTGCAGGGTGATGATCAGGTTGTGGaa                                                                                                                                         <  2:85567/84‑1 (MQ=255)                                                                         gTTATCTTCGTGAGCCATCGACAGGTAGACGATGGTCAGCATCATGAAGATGAAAGCTTGCAGGGTGATGATCAGGTTGTGGaa                                                                                                                                         >  1:85567/1‑84 (MQ=255)                                                                          ttATCTTCGTGAGCCATCGACAGGTAGACGATGGTCAGCATCATGAAGATGAAAGCTTGCAGGGTGATGATCAGGTTGTGGAACACAGCCCACGCCCATTGCAGCACCACACCCAGGCCGCTGAGCCACAGCAGGCCGGAGCCGAACAtt                                                                      >  1:275706/1‑150 (MQ=255)                                                                             tCTTCGTGAGCCATCGAGAGGTAGGGGATGGTCCGCCTATTGTGCCTGGTCCCTTGCATCTTTAGTATCAGGTTTTGGAACACAGCCCACGCCCATTGCAGCACCACACACCGGCCGCTGAGCCACCGCAGGCCGGAGCCGAATATTACa                                                                   <  2:592033/150‑1 (MQ=255)                                                                             tCTTCGTGAGCCATCGACAGGTAGACGATGGTCAGCATCATGAAGATGAAAGCTTTTAGGGTGATGATCAGGTTGTGGAACACAGCCCACGCCCATTGCAGCACCACACCCAGGCCGCTGAGCCACAGCAGGCCGGAGCCGAACATTACa                                                                   <  2:1242857/150‑1 (MQ=255)                                                                                     aGCCATCGACAGGTAGACGATGGTCAGCATCATGAAGATGAAAGCTTGCAGGGTGATGATCAGGTTGTGGAACAGAGCACACGCCCATTGCAGCACCACACCCAGGCCGCTGAGCCACAGCAGGCCGGAGCCGAACATTACAGCGATCAg                                                           >  2:217303/1‑150 (MQ=255)                                                                                                 gTAGACGATGGTCAGCATCATGAAGATGAAAGCTTGCAGGGTGATGATCAGGTTGTGGAACACAGCCCACGCCCATTGCAGCACCACACCCAGGCCGCTGAGCCACAGCAGGCCGGAGCCGAACATTACAGCGATCAGGATGAACACCAg                                               >  1:303361/1‑150 (MQ=255)                                                                                                       gATGGTCAGCATCATGAAGATGAAAGCTTGCAGGGTGATGATCAGGTTGTGGAACACAGCACACGCGCATTGCAGCACCACACCCAGGCCGCTGAGCCACAGAAGGCCGGAGCCGAACATTACAGCGATCAGGATGAACACAAGCTCGcc                                         >  1:296654/1‑150 (MQ=255)                                                                                                           gTCAGCATCATGAAGATGAAAGCTTGCAGGGTGATGATCAGGTTGTGGAACACAGCCCACGCCCATTGCAGCACCACACCCAGGCCGCTGAGCCACAGCAGGCCGGAGCCGAACATTACa                                                                   <  1:1182510/120‑1 (MQ=255)                                                                                                           gTCAGCATCATGAAGATGAAAGCTTGCAGGGTGATGATCAGGTTGTGGAACACAGCCCACGCCCATTGCAGCACCACACCCAGGCCGCTGAGCCACAGCAGGCCGGAGCCGAACATTACa                                                                   >  2:1182510/1‑120 (MQ=255)                                                                                                           gTCAGCATCATGAAGATGAAAGCTTGCAGGGTGATGATCAGGTTGTGGAACACAGCCCACGCCCATTGCAGCACCACACCCAGGCCGCTGAGCCACAGCAGGCCGGAGCCGAACATTACAGCGATCAGGATGAACACCAGCTCGCCAGCa                                     <  2:1136680/150‑1 (MQ=255)                                                                                                            tCAGCATCATGAAGATGAAAGCTTGCAGGGTGATGATCAGGTTGTGGAACACAGCCCACGCCCATTGCAGCACCACACCCAGGCCGCTGAGCCACAGCAGGCCGGAGCCGAACATTACAGCGATCAGGATGAACACCAGCTCGCCAGCAt                                    >  1:1055171/1‑150 (MQ=255)                                                                                                                     tGAAGATGAAAGCTTGCAGGGTGATGATCAGGTTGTGGAACACAGCCCACGCCCATTGCAGCACCACACCCAGGCCGCTGAGCCACAGCAGGCCGGAGCCGAACATTACAGCGATCAGGATGAACACCAGCTCGCCAGCATACATGTTGc                           <  2:761666/150‑1 (MQ=255)                                                                                                                           tcaaaCCTTGCAGAGTTATGATCAGGTTGTCGAACACAGCCCACGCCCATTGCAGCACCACACCCAGTCCGCTGATCCACACCAGGCCGTTGCCGAACATTACAGCCATCAGGATGAACACCAGCTCGCCATCATACATCTTGCCGAACa                     <  2:296654/148‑1 (MQ=255)                                                                                                                            gAAAGCTTGCAGGGTGATGATCAGGTTGTGGAACACAGCCCACGCCCATTGCAGCACCACACCCAGGCCGCTGAGCCACAGCAGGCCGGAGCCGAACATTACAGCGATCAGGATGAACACCAGCTCGCCAGCATACATGTTGCCGAACAg                    >  1:134729/1‑150 (MQ=255)                                                                                                                             aaaGCTTGCAGGGTGATGATCAGGTTGTGGAACACAGCCCACGCCCATTGCAGCACCACACCCAGGCCGCTGAGCCACAGCAGGCCGGAGCCGAACATTACAGCGATCAGGATGAACACCAGCTCGCCAGCATACATGTTGCCGAACAGa                   >  2:87267/1‑150 (MQ=255)                                                                                                                             aaaGCTTGCAGGGTGATGATCAGGTTGTGGAACACAGCCCACGCCCATTGCAGCACCACACCCAGGCCGCTGAGCCACAGCAGGCCGGAGCCGAACATTACAGCGATCAGGATGAACACCAGCTCGCCAGCATACATGTTGCCGAACAGa                   >  1:721616/1‑150 (MQ=255)                                                                                                                                gCTTGCAGGGTGATGATCAGGTTGTGGAACATAGCCCACGCCCATTGCAGCACCACACCCAGGCCGCTGAGCCACAGCAGGCCGGAGCCGAACATTACAGCGATCAGGATGAACACCAGCTCGCCAGCATACATGTTGCCGAACAGACGc                <  1:686723/150‑1 (MQ=255)                                                                                                                                  ttGCAGGGTGATGATCAGGTTGTGGAACACAGCCCACGCCCATTGCAGCACCACACCCAGGCCGCTGAGCCACAGCAGGCCGGAGCCGAACATTACAGCGATCCGGATGAACACCAGCTCGCCAGCATACGTGTTGCCGAACAGACGCAg              <  1:294055/150‑1 (MQ=255)                                                                                                                                  ttGCAGGGTGATGATCAGGTTGTGGAACACAGCCCACGCCCATTGCAGCACCACACCCAGGCCGCTGAGCCACAGCAGGCCGGAGCCGAACATTACAGCGATCAGGATGAACACCAGCTCGCCAGCATACATGTTGCCGAACAGACGCAg              >  2:466950/1‑150 (MQ=255)                                                                                                                                          tgatgaTCAGGTTGTGGAACACAGCCCACGCCCATTGCAGCACCACACCCAGGCCGCTGAGCCACAGCAGGCCGGAGCCGAACATTACAGCGATCAGGATGAACACCAGCTCGCCAGCATACATGTTGCCGAAc                      >  1:639897/1‑134 (MQ=255)                                                                                                                                          tgatgaCCAGGTTGTGGAACACAGCCCACGCCCATTGCAGCACCACACCCAGGCCGCTGAGCCACAGCAGGCCGGAGCCGAACATTACAGCGATCAGGATGAACACCAGCTCGCCAGCATACATGTTGCCGAAc                      <  2:639897/134‑1 (MQ=255)                                                                                                                                           gatgatCAGGTTGTGGAACACAGCCCACGCCCATTGCAGCACCACACCCAGGCCGCTGAGCCACAGCAGGCCGGAGCCGAACATTACAGCGATCAGGATGAACACCAGCTCGCCAGCATACATGTTGCCGAACAGACGCAGTGCCAGCga     <  1:87267/150‑1 (MQ=255)                                                                                                                                           gatgatCAGGTTGTGGAACACAGCCCACGCCCATTGCAGCACCACACCCAGGCCGCTGAGCCACAGCAGGCCGGAGCCGAACATTACAGCGATCAGGATGAACACCAGCTCGCCAGCATACATGTTGCCGAACAGACGCAGTGCCAGCga     >  1:309364/1‑150 (MQ=255)                                                                                                                                              gatCAGGTTGTGGAACACAGCCCACGCCCATTGCAGCACCACACCCAGGCCGCTGAGCCACAGCAGGCCGGAGCCGAACATTACAGCGATCAGGATGAACACCAGCTCGCCAGCATACATGTTGCCGAACAGACGCAGTGCCAGCGAGAt  >  2:68209/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                               CTTGCGGACTTTTTAAGCCCTCCCCCCGTACAGGCAGGGAAGGCTATCAGACACGCCAGGCG‑GTCTTAATGGTTATCTTCGTGAGCCATCGACAGGTAGACGATGGTCAGCATCATGAAGATGAAAGCTTGCAGGGTGATGATCAGGATGTGGAACACAGCCCACGCCCATTGCAGCACCACACCCAGGCCGCTGAGCCACAGCAGGCCGGAGCCGAACATTACAGCGATCAGGATGAACACCAGCTCGCCAGCATACATGTTGCCGAACAGACGCAGTGCCAGCGAGAT  >  NC_002947/6180568‑6180857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑