| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,648,882 | +T | intergenic (‑119/+177) | oprB‑II ← / ← PP_1446 | carbohydrate‑selective porin/TonB‑dependent receptor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,648,881 | 1 | . | T | 98.0% | 158.7 / ‑4.2 | 50 | intergenic (‑118/+178) | oprB‑II/PP_1446 | carbohydrate‑selective porin/TonB‑dependent receptor |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base T (25/24); total (26/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATGTAGCAGGTTTTAGGCAGTTGGAACATGAGATAGCGCTATCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAG‑GA‑TATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGG > NC_002947/1648734‑1649020 | gATGTAGCAGGTTTTAGGCAGTTGGAACATGAGATAGCGCTATCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAG‑Ga‑tt > 2:346238/1‑149 (MQ=255) tAGCAGGTTTTAGGCAGTTGGAACATGAGATAGCGCTATCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAG‑Ga‑ttatag < 2:764078/150‑6 (MQ=255) aGCAGGTTTTAGGCAGTTGGAACATGAGATAGCGCTATCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAG‑GATTATAgg > 2:392717/1‑150 (MQ=255) cAGGTTTTAGGCAGTTGGAACATGAGATAGCGCTATCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAG‑GATTATAgggg > 1:1036420/1‑150 (MQ=255) cAGGTTTTAGGCAGTTGGAACATGAGATAGCGCTATCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAG‑GATTATAgggg > 2:1440916/1‑150 (MQ=255) ttttAGGCAGTTGGAACATGAGATAGCGCTATCTTTTGATTATTGAAAAATACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGGTCGATGAAGCGTTTCAGCTTGCGGCGGGGAG‑GATTATAGGGGAAGt > 1:1463756/1‑150 (MQ=255) ttttAGGCAGTTGGAACATGAGATAGCGCTATCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAG‑Ga‑tta > 2:904652/1‑138 (MQ=255) ttttAGGCAGTTGGAACATGAGATAGCGCTATCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAG‑Ga‑tta < 1:904652/140‑3 (MQ=255) ttAGGCAGTTGGAACATGAGATAGCGCTATCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAG‑GATTATAGGGGAAGTta < 1:392717/150‑1 (MQ=255) tAGGCAGTTGGAACATGAGATAGCGCTATCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAG‑GATTATAGGGGAAGTtat < 2:928182/150‑1 (MQ=255) ttGGAACATGAGATAGCGCTATCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAG‑GATTAAAGGGGAAGTTATAAGaaaa > 2:71161/1‑150 (MQ=255) aTGAGATAGCGCTATCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAG‑GATTATAGGGGAAGTTATAAGAAAAAAGACaa < 1:128663/150‑1 (MQ=255) aTGAGATAGCGCTATCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAG‑GATTATAGGGGAAGTTATAAGAAAAAAGACaa > 2:1450950/1‑150 (MQ=255) aTAGCGCTATCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAG‑GATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGt < 2:1017353/150‑1 (MQ=255) aTAGCGCTATCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAG‑GATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGt > 1:1017353/1‑150 (MQ=255) cgcTATCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAG‑GATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCg > 2:218000/1‑148 (MQ=255) cgcTATCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAG‑GATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCg < 1:218000/148‑1 (MQ=255) ttttGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAG‑GATTATAgg < 2:651672/111‑1 (MQ=255) ttttGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAG‑GATTATAgg > 1:651672/1‑111 (MQ=255) gATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAG‑GATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTc > 1:233072/1‑136 (MQ=255) gATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAG‑GATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTc < 2:233072/136‑1 (MQ=255) attattGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAG‑GATTAAAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGAttgttgt < 1:71161/150‑1 (MQ=255) ttattaAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAG‑GATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGAttgttgtt < 2:1079328/150‑1 (MQ=255) ttattGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAG‑GATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGAttgttgtt > 2:1352999/1‑150 (MQ=255) ttGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAG‑GATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGaa > 2:811615/1‑150 (MQ=255) aaaaaaCGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAG‑GATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACggg < 2:1569774/150‑1 (MQ=255) gCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAG‑GATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAgtc > 1:563215/1‑150 (MQ=255) aaGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAG‑GATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGAttgtt > 2:1320309/1‑130 (MQ=255) aaGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAG‑GATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGAttgtt < 1:1320309/130‑1 (MQ=255) gcgcATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAG‑GATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCa > 1:422623/1‑150 (MQ=255) gcgcATCGAAGGGCGGGCCCCTCAAGGGATATGGCGACAAGATTGTTCGATGAAGCGTTGTAGCTTGCGGCGGGGAG‑GATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCa < 1:1197667/150‑1 (MQ=255) cgcATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGGAGATGTGAG‑TATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCAt < 1:1450950/150‑1 (MQ=255) aaGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAG‑GATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGa < 2:422623/150‑1 (MQ=255) aaGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAG‑GATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGa > 1:209124/1‑150 (MQ=255) aGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAG‑GATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAg < 2:209124/150‑1 (MQ=255) gggcgggcCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAG‑GATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAgg < 1:1352999/150‑1 (MQ=255) cgggcCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAG‑GATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAg < 2:993099/150‑1 (MQ=255) ggcCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAG‑GATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAgat > 2:1421256/1‑148 (MQ=255) gcCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAG‑GATTATAGGGGAAGTTATAAGAAAAAAGACaa < 2:575292/93‑1 (MQ=255) gcCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAG‑GATTATAGGGGAAGTTATAAGAAAAAAGACaa > 1:575292/1‑93 (MQ=255) cTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAG‑GATTATAGGGGAAGTTATAAGaaaaaa < 1:1239580/84‑1 (MQ=255) cTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAG‑GATTATAGGGGAAGTTATAAGaaaaaa > 2:1239580/1‑84 (MQ=255) cTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAG‑GATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAgg > 2:1172625/1‑150 (MQ=255) tCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGggag‑ga > 2:533414/1‑58 (MQ=255) tCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGggag‑ga < 1:533414/58‑1 (MQ=255) aTGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAG‑GATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCg > 1:5593/1‑150 (MQ=255) ggATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAG‑GATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCggg < 1:1421256/150‑1 (MQ=255) ggATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAG‑GATTATAAGGGAAGTAGTCAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCggg < 1:811615/150‑1 (MQ=255) atatGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAG‑GATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGtt > 2:459468/1‑150 (MQ=255) aaGCTTGTTCGATGAAGCGTTTCAGGTTGCGGCGGGGAG‑GATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGaa < 2:1269249/150‑1 (MQ=255) gATGAAGCGTTTCAGCTTGCGGCGGGGAGAAA‑TATAGGGGAAGTTATAAGAAAAAAGACAAATTTTCGCAGAGGATTGTAGTTGAACGGGCAACACTCCTCATTAATCGAGGCAAGTGTAGAAGAGGGTTAACCAGCAAAAGCGcccatt > 2:148894/1‑146 (MQ=255) gAAGCGTTTCAGCTTGCGGCGGGGAG‑GATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGcc > 2:1425276/1‑150 (MQ=255) ttCAGCTTGCGGCGGGGAG‑GATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCAt > 2:682402/1‑150 (MQ=255) ttGCGGCGGGGAG‑GATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACAATCGTAtt > 2:1318820/1‑150 (MQ=255) ggcggGGAG‑GATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTcgcg < 2:774721/150‑1 (MQ=255) gcggGGAG‑GATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCgg < 1:1172625/150‑1 (MQ=255) | GATGTAGCAGGTTTTAGGCAGTTGGAACATGAGATAGCGCTATCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAG‑GA‑TATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGG > NC_002947/1648734‑1649020 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |