| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,162,654 | Δ1 bp | 12.8% | intergenic (+129/+72) | PP_4544 → / ← PP_4545 | hypothetical protein/beta‑ketodecanoyl‑ACP synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,162,654 | 0 | G | . | 12.8% | 123.5 / 16.5 | 47 | intergenic (+129/+72) | PP_4544/PP_4545 | hypothetical protein/beta‑ketodecanoyl‑ACP synthase |
| Reads supporting (aligned to +/- strand): ref base G (16/25); new base . (2/4); total (18/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.28e-02 | |||||||||||
CCTGGAAGCCAAGTTCTAAGCAACACCCTGCTCTGCTTCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACC‑TTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCT > NC_002947/5162507‑5162798 | ccTGGAAGCCAAGTTCTAAGCAACACCCTGCTCTGCTTCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGAc < 1:956342/150‑1 (MQ=255) ggAAGCCAAGTTCTAAGCAACACCCTGCTCTGCTTCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACC‑tt < 1:905445/150‑1 (MQ=255) gcagggcctgttccgcagtgggcatgacgtttttgttcaccaggatttccgaacgcaggaccatggccgttcctctgtgtttattcagtggggtaggggtttgggccttcgcgggcacgcccgctcccacaggaaCACCACA‑acc‑tttga > 2:1136499‑M2/136‑147 (MQ=255) aaGCCAAGTTCTAAGCAACCCCCTGCTCTGCTTCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACC‑TTga < 2:1249742/150‑1 (MQ=255) ccAAGTTCTAAGCAACACCCTGCTCTGCTTCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACC‑TTGAGaa < 2:128613/150‑1 (MQ=255) ccAAGTTCTAAGCAACACCCTGCTCTGCTTCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACC‑TTGAGaa > 2:1187552/1‑150 (MQ=255) gTTCTAAGCAACACCCTGCTCTGCTTCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACC‑TTGAGAACtgt < 2:1155074/150‑1 (MQ=255) cgcagtgggcatgacgtttttgttcaccaggatttccgaacgcaggaccatggccgttcctctgtgtttattcagtggggtaggggtttgggccttcgcgggcacgcccgctcccacaggaaCACCACA‑ACCTTTGAAACCTGTGCTGTa < 1:1136499‑M2/28‑1 (MQ=255) cacCCTGCTCTGCTTCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACC‑TTga < 2:829999/132‑1 (MQ=255) cacCCTGCTCTGCTTCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACC‑TTga > 1:829999/1‑132 (MQ=255) gcatgacgtttttgttcaccaggatttccgaacgcaggaccatggccgttcctctgtgtttattcagtggggtaggggtttgggccctttcgcgggcacgcccgctcccacaggaaCACCACA‑ACCTTTGAAACCTGTGCTGTACCtgtg < 1:517440‑M2/34‑1 (MQ=255) tctgctTCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACC‑TTGAGAACTGTGCTGTACctgtgggagcgg > 1:324372‑M2/1‑138 (MQ=255) ctgctTCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACC‑TTGAGAACTGTGCTGTACctgtgggagcggg > 2:415037‑M2/1‑137 (MQ=255) ctTCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACC‑TTGAGAACTGTGCTGTACctgtgggagcgggcaa > 2:805113‑M2/1‑134 (MQ=255) ttCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCAAATTAGCACCACAGACC‑TTGAGAACTGTGCTGTACctgtgggagcgggcaag < 1:1243229‑M2/150‑18 (MQ=255) tGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACC‑TTGAGAACTGTGCTGTACctgtgg > 1:1023692‑M2/1‑127 (MQ=255) tGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACC‑TTGAGAACTGTGCTGTACctgtgg < 2:1023692‑M2/133‑7 (MQ=255) gCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACC‑TTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAaga < 1:716400/150‑1 (MQ=255) cGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACC‑TTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAagag < 2:195728/150‑1 (MQ=255) aaCGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACC‑TTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGt < 2:550054/150‑1 (MQ=255) gCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACC‑TTGAGAACTGTGCTGTACctgtgggagcgggcaagcccgc > 1:1203165‑M2/1‑114 (MQ=255) gCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACC‑TTGAGAACTGTGCTGTACctgtgggagcgggcaagcccgc < 2:1203165‑M2/136‑23 (MQ=255) aacgcaggaccatggccgttcctctgtgtttattcagtggggtaggggtttgggcctttcgcgggcacgcccgctcccacaggaaCACCACA‑ACCTTTGAAACCTGTGCTGTACCTGTGGGAGCGGGCATGccc < 2:1267835‑M2/49‑1 (MQ=255) aacgcaggaccatggccgttcctctgtgtttattcagtggggtaggggtttgggcctttcgcgggcacgcccgctcccacaggaaCACCACA‑ACCTTTGAAACCTGTGCTGTACCTGTGGGAGCGGGCATGccc > 1:1267835‑M2/86‑134 (MQ=255) aCCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACC‑TTGAGAACTGTGCTGTACCTGGGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGgc < 1:1187552/150‑1 (MQ=255) aggaccatggccgttcctctgtgtttattcagtggggtaggggtttgggccttcgcgggcacgcccgctcccacaggaaCACCACA‑ACCTTTGAAACCTGTGCTGTACCTGTGGGAGCGGGcat > 1:768273‑M2/80‑123 (MQ=255) aggaccatggccgttcctctgtgtttattcagtggggtaggggtttgggccttcgcgggcacgcccgctcccacaggaaCACCACA‑ACCTTTGAAACCTGTGCTGTACCTGTGGGAGCGGGcat < 2:768273‑M2/45‑2 (MQ=255) tCCGGCGTTTCCCGTTTCTTTTTGTCTGTGTGCACGGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACC‑TTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCtga < 1:6461/150‑1 (MQ=255) tCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACC‑TTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCtga > 1:752501/1‑150 (MQ=255) ccGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGTCCCCATCGCGGGCTTGTCCGCTCCCACAGTATCACCACAGACC‑TTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCtgat < 2:875295/150‑1 (MQ=255) tttCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACC‑TTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCa < 2:752501/150‑1 (MQ=255) tttCCCGCTTCTTTTTGTCCGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGCCC‑TTGAAAACTGGTCTGTACCTGTGGGAGCGGCCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCa < 1:1242118/150‑1 (MQ=38) ttCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCATAGTATCACCACAGACC‑TTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAg > 1:80573/1‑150 (MQ=255) aTGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACC‑TTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAAt < 2:282399/150‑1 (MQ=255) cccTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACC‑TTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGTCCGTAACAGgcgc < 1:127780/102‑1 (MQ=255) cccTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACC‑TTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGTCCGTAACAGgcgc > 2:127780/1‑102 (MQ=255) gcgGGCTTGCCCGCTCCCACAGTATCACCACAGACC‑TTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCa > 2:752802/1‑150 (MQ=255) ggCTTGCCCGCTCCCAACGTATCACACCAGAGC‑TTCAGAAATGTGATGTACGGGTGGGAGCTGGCATGCCCGCGAAGAGGCCGTAACAGGCGAATGATGCATCAGCGCTCGAGAAGAAACATACTACCAATCGAGCACCCCGCACCAAAc < 2:1243229/150‑1 (MQ=255) ccGCTCCCACAGTATCACCACAGACC‑TTGAGAACTGTGCTGTACctgtgggagcgggcaagcccgcgaaggggccggcgcatttcacacagacaaaaagaagcgggaaacgccggatggggtcggcgtttcccgcttcaggtgaagcaga < 2:677527‑M2/150‑107 (MQ=255) ccGCTCCCACAGTATCACCACAGACC‑TTGAGAACTGTGCTGTACCTGTGTGAACGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCa > 1:4036/1‑150 (MQ=255) ccGCTCCCACAGTATCACCACAGACC‑TTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAAt > 1:678013/1‑124 (MQ=255) ccGCTCCCACAGTATCACCACAGACC‑TTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAAt < 2:678013/124‑1 (MQ=255) ccGCTCCCACAGTATCACCACAGACC‑TTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCa > 1:68173/1‑150 (MQ=255) gCTCCCACAGTATCACCACAGACC‑TTGAGAACTGTGCTGTACctgtgggagcgggcaagcccgcgaaggggccggcgcatttcacacagacaaaaagaagcgggaaacgccggatggggtcggcgttt < 2:1145070‑M2/128‑87 (MQ=255) gCTCCCACAGTATCACCACAGACC‑TTGAGAACTGTGCTGTACctgtgggagcgggcaagcccgcgaaggggccggcgcatttcacacagacaaaaagaagcgggaaacgccggatggggtcggcgttt > 1:1145070‑M2/1‑42 (MQ=255) tCCCACAGTATCACCACAGACC‑TTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAacac < 1:752802/150‑1 (MQ=255) accacAGACC‑TTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGcc > 1:233934/1‑150 (MQ=255) ccacAGACC‑TTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCt < 2:80573/150‑1 (MQ=255) | CCTGGAAGCCAAGTTCTAAGCAACACCCTGCTCTGCTTCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACC‑TTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCT > NC_002947/5162507‑5162798 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |