Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	503,852	C→T	12.8%	intergenic (+52/‑99)	PP_0414 → / → rpe	polyamine ABC transporter permease/ribulose‑5‑phosphate 3‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	503,852	0	C	T	12.8%	129.4 / 8.2	55	intergenic (+52/‑99)	PP_0414/rpe	polyamine ABC transporter permease/ribulose‑5‑phosphate 3‑epimerase
Reads supporting (aligned to +/- strand):  ref base C (24/22);  new base T (4/3);  total (30/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTATTGCCCCCTCCATTCTCTCTGCCGATTTC  >  NC_002947/503719‑503995                                                                                                                                       |                                                                                                                                                tGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGTCGGCACAGGTTGCGTTGGTc                                                                                                                                 <  2:454255/150‑1 (MQ=255) tGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTc                                                                                                                                 <  1:1233977/150‑1 (MQ=255) tGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTACTGGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTc                                                                                                                                 <  1:1030708/150‑1 (MQ=255)   cGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAATGCCG‑TAAGAGGCCGGCACAGGTTGCGTTGGTCGa                                                                                                                               <  1:887250/150‑1 (MQ=255)    gTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGtt                                                                                                                                     <  2:371042/143‑1 (MQ=255)    gTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGtt                                                                                                                                     >  1:371042/1‑143 (MQ=255)           gtgtTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAAtt                                                                                                                       <  2:54617/150‑1 (MQ=255)                gttgCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATAc                                                                                                                  <  2:953157/150‑1 (MQ=255)                 tgtctgctgACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATAcc                                                                                                                 >  2:320691/4‑150 (MQ=255)                    ctgctgACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGCCTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAAt                                                                                                              <  2:793069/150‑1 (MQ=255)                         gACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGGGCAGGTTGCGTTGGTCGACACCAATTGATACCAATTAGcc                                                                                                         >  1:1135582/1‑150 (MQ=255)                               ggAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATc                                                                                                   >  2:1124454/1‑150 (MQ=255)                                          gTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGa                                                                                        >  2:569722/1‑150 (MQ=255)                                          gTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGa                                                                                        <  1:320691/150‑1 (MQ=255)                                               catctcGAGAATCTTAGGACTCAACAGCCGGCTTCATGATGTCGTGCCTGTACCGGCCTCTTCGCCGCTAAAGCCT‑TAAGAGGCCGGCACAGGTTGCGTTGGTCTACACCACTTACTGCCAATCAGCCTTCATCGCTTTCCCTGAGTTAc                                                                                   <  1:1066639/145‑1 (MQ=255)                                                gTAGCGAGAAGCTGAGGACTCAACAGACGGCTTGATGATGGCTTGCCTTTACCGGCCACTTCGCGGCTAAAGCCG‑GAAGAGGCCGGGACAGGTTGCGTTGATCGACACCAACTGCAACAAATTAACGCTCATCGCTTACCCTGAGATACa                                                                                  >  1:301734/1‑150 (MQ=255)                                                     gagaAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGaca                                                                                                                             <  1:1209363/102‑1 (MQ=255)                                                     gagaAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGaca                                                                                                                             >  2:1209363/1‑102 (MQ=255)                                                     gagaAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATAGCAATGTGCGTTAATCGCTTTCCCTGAGTTACaagatt                                                                             >  2:1066639/1‑146 (MQ=255)                                                     gagaAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCaa                                                                                                               <  1:217654/116‑1 (MQ=255)                                                     gagaAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCaa                                                                                                               >  2:217654/1‑116 (MQ=255)                                                     gagaAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATgcg                                                                             >  2:328790/1‑150 (MQ=255)                                                      agaAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATgcgc                                                                            <  2:181935/150‑1 (MQ=255)                                                      agaAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATgcgc                                                                            <  1:328790/150‑1 (MQ=255)                                                                  cTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAAt                                                                <  1:569722/150‑1 (MQ=255)                                                                   tCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTg                                                                                                                                    <  2:68229/81‑1 (MQ=255)                                                                   tCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAAtt                                                               >  1:951818/1‑150 (MQ=255)                                                                   tCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCAAAGGTTGCGTTg                                                                                                                                    >  1:68229/1‑81 (MQ=255)                                                                    cAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAAttt                                                              <  2:691225/150‑1 (MQ=255)                                                                     gacggtgggCTTGATGATGGCGTGCCTGTACCGGCCTCTTCGCGGCTAAAGCAG‑CGAGAGGTCGGTACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGTTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTg                                                             <  2:301734/143‑1 (MQ=255)                                                                           cGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACa                                                       >  2:1088383/1‑150 (MQ=255)                                                                             gCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCg                                                                                                  >  2:1198374/1‑105 (MQ=255)                                                                             gCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCg                                                                                                  <  1:1198374/105‑1 (MQ=255)                                                                                    tatgGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCa                                              >  1:936098/2‑150 (MQ=255)                                                                                      aacgcaaccTGTGCAGGCCTCTTC‑CGGCTTTAGCCGCGAAGAGGCCGGTACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATg                                            >  2:212274/8‑150 (MQ=25)                                                                                        gCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATAGCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACa                                                       <  2:815735/137‑1 (MQ=255)                                                                                        gCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACa                                                       >  1:815735/1‑137 (MQ=255)                                                                                        cgcaaccTGTGCCGGCCTCTTC‑CGGCTTTAGCCGCGAAGAGGCCGGTACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATACa                                          >  2:80891/6‑150 (MQ=25)                                                                                            gCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGt                                                                    >  1:474111/1‑120 (MQ=255)                                                                                            gCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGt                                                                    <  2:474111/120‑1 (MQ=255)                                                                                             ccTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTTCCAACAGTTTCGCCATGCAGCCCt                                     >  2:133485/1‑150 (MQ=255)                                                                                             ccTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCt                                     >  1:871274/1‑150 (MQ=255)                                                                                              cTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTa                                    >  1:502633/1‑150 (MQ=255)                                                                                               tGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTAc                                   >  1:935705/1‑150 (MQ=255)                                                                                                 tACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGc                                 >  1:749665/1‑150 (MQ=255)                                                                                                     ggCCTCTTC‑CGGCTTTAGCCGCGAAGAGGCCGGTACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTAtt                             <  1:212274/150‑1 (MQ=38)                                                                                                      gCCTCTTC‑CGGCTTTAGCCGCGAAGAGGCCGGTACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAAt                                                                                >  1:730177/1‑98 (MQ=25)                                                                                                      gCCTCTTC‑CGGCTTTAGCCGCGAAGAGGCCGGTACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAAt                                                                                <  2:730177/98‑1 (MQ=25)                                                                                                      gCCTCTTC‑CGGCTTTAGCCGCGAAGAGGCCGGTACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTATTg                            >  1:501310/1‑150 (MQ=38)                                                                                                           ttCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGACTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTATTGccccc                       >  1:341015/1‑150 (MQ=255)                                                                                                            tCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTATTGCCCCCt                      <  2:749665/150‑1 (MQ=255)                                                                                                              gcgGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTATTGCCCCCTcc                    >  1:475183/1‑150 (MQ=255)                                                                                                                       gCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTATTGCCCCCTCCATTctctct           >  2:164356/1‑150 (MQ=255)                                                                                                                            gAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTATTGCCCCCTCCATTCTCTCTGCCg       >  2:866956/1‑150 (MQ=255)                                                                                                                                 ggCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTATTGCCCCCTCCATTCTCTCTGCCGATTTc  <  1:1088383/150‑1 (MQ=255)                                                                                                                                       |                                                                                                                                                TGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTATTGCCCCCTCCATTCTCTCTGCCGATTTC  >  NC_002947/503719‑503995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑