Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,024,096 C→T 6.0% G352S (GGC→AGC)  opdP ← glycine‑glutamate dipeptide porin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,024,0960CT 6.0% 190.6 / 2.1 67G352S (GGC→AGC) opdPglycine‑glutamate dipeptide porin
Reads supporting (aligned to +/- strand):  ref base C (31/32);  new base T (2/2);  total (33/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.95e-01

CAGGCCATTCACGTCGTAGGCAGTGCCGCGGTAGTGGGTGCCGTCGATACCCCAGCCGCGGGCGTTGTACAGGTTGAACTTCAGGCCAGGCACGCCATAGGGCGCCATGTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGTTGGTTTCGTGCAGGTAGTCGAAGTACTCATTGCCGTTGACCTGCTGCCAGGAGGCGCTGAGGGTGTGGGCTTGATGGGTCAGGCCCAGTGACAGGCTGTAAGTGTC  >  NC_002947/1023947‑1024243
                                                                                                                                                     |                                                                                                                                                   
cAGGCCATTCACGTCGTAGGCAGTGCCGCGGTAGTGGGTGCCGTCGATACCCCAGCCGCGGGCGTTGTACAGGTTGAACTTCAGGCCAGGCACGCCATAGGGCGCCATGTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGcc                                                                                                                                                     >  2:374727/1‑150 (MQ=255)
  ggCCATTCACGTCGTAGGCAGTGCCGCGGTAGTGGGTGCCGTCGATACCCCAGCCGCGGGCGTTGTACAGGTTGAACTTCAGGCCAGGCACGCCATAGGGCGCCATGTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGt                                                                                                                                                   >  2:132166/1‑150 (MQ=255)
   gCCATTCACGTCGTAGGCAGTGCCGCGGTAGTGGGTGCCGTCGATACCCCAGCCGCGGGCGTTGTACAGGTTGAACTTCAGGCCAGGCACGCCATAGGGCGCCATGTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGtt                                                                                                                                                  >  1:939502/1‑150 (MQ=255)
        tCACGTCGTAGGCAGTGCCGCGGTAGTGGGTGCCGTCGATACCCCAGCCGCGGGCGTTGTACAGGTTGAACTTCAGGCCAGGCACGCCATAGGGCGCCATGTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGt                                                                                                                                             <  1:374727/150‑1 (MQ=255)
         ggcgtcgtAGGCAGTGCCGCGGTAGTGGGTGCCGTCGATACCCCAGCAGCGGGCGTTGTACAGGTTGAACTTCAGGCCAGGCACGCCATAGGGCGCCATGTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTc                                                                                                                                            <  1:782288/148‑1 (MQ=255)
            gtcgtAGGCAGTGCCGCGGTAGTGGGTGCCGTCGATACCCCAGCCGCGGGCGTTGTACAGGTTGAACTTCAGGCCAGGCACGCCATAGGGCGCCATGTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGa                                                                                                                                         <  2:764764/150‑1 (MQ=255)
                 aGGCAGTGCCGCGGTAGTGGGTGCCGTCGATACCCCAGCCGCGGGCGTTGTACAGGTTGAACTTCAGGCCAGGCACGCCATAGGGCGCCATGTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGAcagca                                                                                                                                    <  2:848765/150‑1 (MQ=255)
                          cgcgGTAGTGGGTGCCGTCGATACCCCAGCCGCGGGCGTTGTACAGGTTGAACTTCAGGCCAGGCACGCCATAGGGCGCCATGTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTgg                                                                                                                           >  2:1022120/1‑150 (MQ=255)
                               tAGTGGGTGCCGTCGATACCCCAGCCGCGGGCGTTGTACAGGTTGAACTTCAGGCCAGGCACGCCATAGGGCGCCATGTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAgg                                                                                                                      <  1:230961/150‑1 (MQ=255)
                                 gTGGGTGCCGTCGATACCCCAGCCGCCCAGGGTGTACAGGTTGAACTTCAGGCCAGGCACGCCATAGGGCGCCATGTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGaa                                                                                                                    >  1:674711/1‑150 (MQ=255)
                                     gTGCCGTCGATACCCCAGCCGCGGGCGTTGTACAGGTTGAACTTCGAAAGTGTGACGCCATAGGGCGCCATGTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATg                                                                                                                <  2:674711/150‑1 (MQ=255)
                                          gTCGATACCCCAGCCGCGGGCGTTGTACAGGTTGAACTTCAGGCCAGGCACGCCATAGGGCGCCATGTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGtt                                                                                                           >  2:919865/1‑150 (MQ=255)
                                          gTCGATACCCCAGCCGCGGGCGTTGTAAAGGTTGAACTTCAGGCCAGGCACGCCATAGGGCGCCATGTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGtt                                                                                                           >  2:1213532/1‑150 (MQ=255)
                                                  cccAGCCGCGGGCGTTGTACAGGTTGAACTTCAGGCCAGGCACGCCATAGGGCGCCATGTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGTTGGTTTCGt                                                                                                   <  1:1252741/150‑1 (MQ=255)
                                                      gCCGCGGGTGTTTTACAGGTTGAACTTCAGGCCAGGCACGCCATAGGGCGCCATGTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGTCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGTTGGTTTCGTGCAg                                                                                               <  2:195574/150‑1 (MQ=255)
                                                      gCCGCGGGCGTTGTACAGGTTGAACTTCAGGCCAGGCACGCCATAGGGCGCCATGTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGGTGCCGTTGGTTTCGTGCat                                                                                               <  2:1114755/150‑2 (MQ=255)
                                                               gTTGTACAGGTTGAACTTCAGGCCAGGCACGCCATAGGGCGCCATGTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGTTGGTTTCGTGCAGGTAGTCGaa                                                                                      <  2:807999/150‑1 (MQ=255)
                                                                   tACAGGTTGAACTTCAGGCCAGGCACGCCATAGGGCGCCATGTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGTTGGTTTc                                                                                                     >  1:192433/1‑131 (MQ=255)
                                                                   tACAGGTTGAACTTCAGGCCAGGCACGCCATAGGGCGCCATGTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGTTGGTTTc                                                                                                     <  2:192433/131‑1 (MQ=255)
                                                                    aCAGGTTGAACTTCAGGCCAGGCACGCCATAGGGCGCCATGTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGTTGGTTTCGTGCAGGTAGTCGAAGTACt                                                                                 <  1:1022120/150‑1 (MQ=255)
                                                                     cAGGTTGAACTTCAGGCCAGGCACGCCATAGGGCGCCATGTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTcc                                                                                                                                           >  2:302690/1‑91 (MQ=255)
                                                                     cAGGTTGAACTTCAGGCCAGGCACGCCATAGGGCGCCATGTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTcc                                                                                                                                           <  1:302690/91‑1 (MQ=255)
                                                                        gTTGAACTTCAGGCCAGGCACGCCATAGGGCGCCATGTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGt                                                                                                            <  1:861541/119‑1 (MQ=255)
                                                                        gTTGAACTTCAGGCCAGGCACGCCATAGGGCGCCATGTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGt                                                                                                            >  2:861541/1‑119 (MQ=255)
                                                                        gTTGAACTTCAGGCCAGGCACGCCATAGGGCGCCATGTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGTTGGTTTCGTGCAGGTAGTCGAAGTACTCAtt                                                                             >  2:26597/1‑150 (MQ=255)
                                                                         ttGAACTTCAGGCCAGGCACGCCATAGGGCGCCATGTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGa                                                                                                                  >  2:1263839/1‑112 (MQ=255)
                                                                         ttGAACTTCAGGCCAGGCACGCCATAGGGCGCCATGTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGa                                                                                                                  <  1:1263839/112‑1 (MQ=255)
                                                                          tGAACCTCAGGACAGTCGCGCCATAGGGCGCCATGTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTAGAAGTCCGACAGCAGCAAGTTGTCAAGGTCCATGCCGTTGGTTTCCTGCAGGTAGTCGAAGTACTCATTGc                                                                           <  1:731572/150‑1 (MQ=255)
                                                                            aaCTTCAGGCCAGGCACGCCATAGGGCGCCATGTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGTTGGTTTCGTGCAGGTAGTCGAAGTACTCATTGCCg                                                                         >  1:414073/1‑150 (MQ=255)
                                                                            aaCTTCAGGCCAGGCACGCCATAGGGCGCCATGTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGTTGGTTTCGTGCAGGTAGTCGAAGTACTCATTGCCg                                                                         <  2:414073/150‑1 (MQ=255)
                                                                               ttCAGGCCAGGCACGCCATAGGGCGCCATGTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCg                                                                                                             <  1:1010846/111‑1 (MQ=255)
                                                                               ttCAGGCCAGGCACGCCATAGGGCGCCATGTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCg                                                                                                             >  2:1010846/1‑111 (MQ=255)
                                                                               ttCAGGCCAGGCACGCCATAGGGCGCCATGTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGTTGGTTTCGTGCAGGTAGTCGAAGTACTCATTGCCGTTg                                                                      >  1:712343/1‑150 (MQ=255)
                                                                               ttCAGGCCAGGCACGCCATAGGGCGCCATGTTCAGCACATAGGAAAGCTGAATGGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGTTGGTTTCGTGCAGGTAGTCGAAGTACTCATTGCCGTTg                                                                      >  1:996322/1‑150 (MQ=255)
                                                                                 caggccaggcACGCCATAGGGCGCCATGTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGTTGGTTTCGTGCAGGTAGTCGAAGTACTCATTGCCGTTGAc                                                                    <  2:712343/150‑1 (MQ=255)
                                                                                 caggccaggcACGCCATAGGGCGCCATGTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGTTGGTTTCGTGCAGGTAGTCGAAGTACTCATTGCCGTTGAc                                                                    <  1:132166/150‑1 (MQ=255)
                                                                                   ggccaggcACGCCATAGGGCGCCATGTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGTTGGTTTCGTGCAGGTAGTCGAAGTACTCATTGCCGTTGACct                                                                  >  2:1209467/1‑150 (MQ=255)
                                                                                   ggccaggcACGCCATAGGGCGCCATGTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGTTGGTTTCGTGCAGGTAGTAGAAGTACTCATTGCCGTTGACct                                                                  >  2:122216/1‑150 (MQ=255)
                                                                                    gccaggcACGCCATAGGGCGCCATGTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAg                                                                                                                               <  2:583244/88‑1 (MQ=255)
                                                                                    gccaggcACGCCATAGGGCGCCATGTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAg                                                                                                                               >  1:583244/1‑88 (MQ=255)
                                                                                    gccaggcACGCCATAGGGCGCCATGTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGTTGGTTTCGTGCAGGTAGTCGAAGTACTCATTGCCGTTGACctg                                                                 >  2:311916/1‑150 (MQ=255)
                                                                                      caggcACGCCATAGGGCGCCATGTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGTTGGTTTCGTGCAGGTAGTCGAAGTACTCATTGCCGTTGACctgct                                                               >  2:765828/1‑150 (MQ=255)
                                                                                             gCCATAGGGCGCCATGTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCTGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGTTGGTTTCGTGCa                                                                                                <  1:197616/110‑1 (MQ=255)
                                                                                             gCCATAGGGCGCCATGTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCTGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGTTGGTTTCGTGCa                                                                                                >  2:197616/1‑110 (MQ=255)
                                                                                             gCCATAGGGCGCCATGTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGTTGGTTTCGTGCAGGTAGTCGAAGTACTCATTGCCGTTGACCTGCTGCCagga                                                        <  1:1209467/150‑1 (MQ=255)
                                                                                               cATAGGGCGCCATGTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGTTGGTTTCGTGCAGGTAGTCGAAGTACTCATTGCCGTTGACCTGCTGCCaggagg                                                      >  2:65711/1‑150 (MQ=255)
                                                                                                        ccATGTTCAGCACATAGGAAAGCTGCAGGGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGTTGGTTTCGTGCAGGTAGTCGAAGTACTCATTGCCGTTGACCTGCTGCCAGGAGGCGCTGAggg                                             >  1:676349/1‑150 (MQ=255)
                                                                                                            gTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGTTGGTTTCGTGCAGGTAGTCGAAg                                                                                     >  2:898250/1‑106 (MQ=255)
                                                                                                            gTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGTTGGTTTCGTGCAGGTAGTCGAAg                                                                                     <  1:898250/106‑1 (MQ=255)
                                                                                                             ttCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGTTGGTTTCGTGCAGGTAGTCGAAGTACTCATTGCCGTTGACCTGCTGCCAGGAGGCGCTGAGGGTGTgg                                        >  1:572239/1‑150 (MQ=255)
                                                                                                             ttCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGTTGGTTTCGTGCAGGTAGTCGAAGTACTCATTGCCGTTGACCTGCTGCCAGGAGGCGCTGAGGGTGTgg                                        >  2:904598/1‑150 (MQ=255)
                                                                                                                  cacaTAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCTGGGGTTTCGTGCAGGTAGTCGAAGTACTCATTGCCGTTGACCTGCTGCCAGGAGGCGCTGAGGGTGTGGGCTTg                                   >  1:85870/1‑150 (MQ=255)
                                                                                                                      tAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGATGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGTTGGTTTCGTGCAGGTAGTCGAAGTACTCATTGCCGTTGACCTGCTGCCAGGAGGCGCTGAGGGTGTGGGCTTGATgg                               <  2:939502/150‑1 (MQ=255)
                                                                                                                       aGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGTTGGTTTCGTGCAGGTAGTCGAAGTACTCATTGCCGTTGACCTGCTGCCAGGAGGCGCTGAGGGTGTGGGCTTGATggg                              >  1:831134/1‑150 (MQ=255)
                                                                                                                        ggAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGTTGGTTTCGTGCAGGTAGTCGAAGTACTCATTGCCGTTGACCTGCTGCCAGGAGGCGCTGAGGGTGTGGGCTTGATGGGt                             <  1:122216/150‑1 (MQ=255)
                                                                                                                          aaaGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGTTGGTTTCGTGCAGGTAGTCGAAGTACTCATTGCCGTTGACCTGCTGCCAGGAGGCGCTGAGGGTGTGGGCTTGATGGGTCa                           >  2:549051/1‑150 (MQ=255)
                                                                                                                           aaGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGTTGGTTTCGTGCAGGTAGTCGAAGTACTCATTGCCGTTGACCTGCTGCCAGGAGGCGCTGAGGGTGTGGGCTTGATGGGTCAg                          <  1:1213532/150‑1 (MQ=255)
                                                                                                                             gCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGTTGGTTTCGTGCAGGTAGTCGAAGTACTCATTGCCGTTGACCTGCTGCCAGGAGGCGCTGAGGGTGTGGGCTTGATGGGTCAGGc                        <  1:765828/150‑1 (MQ=255)
                                                                                                                              cTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGTTGGTTTCGTGCAGGTAGTCGAAGTACTCATTGCCGTTGACCTGCTGCCAGGAGGCGCTGAGGgtgt                                          >  1:1237439/1‑131 (MQ=255)
                                                                                                                              cTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGTTGGTTTCGTGCAGGTAGTCGAAGTACTCATTGCCGTTGACCTGCTGCCAGGAGGCGCTGAGGgtgt                                          <  2:1237439/131‑1 (MQ=255)
                                                                                                                              cTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGTTGGTTTCGTGCAGGTAGTCGAAGTACTCATTGCCGTTGACCTGCTGCCAGGAGGCGCTGAGGGTGTGGGCTTGATGGGTCAGGcc                       <  1:904598/150‑1 (MQ=255)
                                                                                                                                 cAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGTTGGTTTCGTGCAGGTAGTCGAAGTACTCATTGCCGTTGACCTGCTGCCAGGAGGCGCTGAGGGTGTGGGCTTGATgg                               <  2:578896/139‑1 (MQ=255)
                                                                                                                                 cAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGTTGGTTTCGTGCAGGTAGTCGAAGTACTCATTGCCGTTGACCTGCTGCCAGGAGGCGCTGAGGGTGTGGGCTTGATgg                               >  1:578896/1‑139 (MQ=255)
                                                                                                                                     gATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGTTGGTTTCGTGCAGGTAGTCGAAGTACTCATTGCCGTTGACCTGCTGCCAGGAGGCGCTGAGGGTGTGGGCTTGATGGGTCAGGCCCAGTGAc                >  1:40611/1‑150 (MQ=255)
                                                                                                                                     gATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGTTGGTTTCGTGCAGGTAGTCGAAGTACTCATTGCCGTTGACCTGCTGCCAGGAGGCGCTGAGGGTGTGGGCTTGATGGGTCAGGCCCAGTGAc                <  1:919865/150‑1 (MQ=255)
                                                                                                                                             gTTGGGGCTGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGTTGGTTTCGTGCAGGTAGTCGAAGTACTCATTGCCGTTGACCTGCTGCCAGGAGGCGCTGAGGgtgt                                          <  1:390254/116‑1 (MQ=255)
                                                                                                                                             gTTGGGGCTGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGTTGGTTTCGTGCAGGTAGTCGAAGTACTCATTGCCGTTGACCTGCTGCCAGGAGGCGCTGAGGgtgt                                          >  2:390254/1‑116 (MQ=255)
                                                                                                                                                   gCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGTTGGTTTCGTGCAGGTAGTCGAAGTACTCATTGCCGTTGACCTGCTGCCAGGAGGCGCTGAGGGTGTGGGCTTGATGGGTCAGGCCCAGTGACAGGCTGTAAGTGTc  <  2:831134/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CAGGCCATTCACGTCGTAGGCAGTGCCGCGGTAGTGGGTGCCGTCGATACCCCAGCCGCGGGCGTTGTACAGGTTGAACTTCAGGCCAGGCACGCCATAGGGCGCCATGTTCAGCACATAGGAAAGCTGCAGCGATTTCTCGTTGGGGCCGTTGAAGTCCGACAGCAGCGAGTTGGCCAGGAAGATGCCGTTGGTTTCGTGCAGGTAGTCGAAGTACTCATTGCCGTTGACCTGCTGCCAGGAGGCGCTGAGGGTGTGGGCTTGATGGGTCAGGCCCAGTGACAGGCTGTAAGTGTC  >  NC_002947/1023947‑1024243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑