Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,047,416 G→T 7.0% A322D (GCC→GAC)  PP_0906 ← RND family multidrug transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,047,4160GT 7.0% 177.7 / 3.0 57A322D (GCC→GAC) PP_0906RND family multidrug transporter
Reads supporting (aligned to +/- strand):  ref base G (28/25);  new base T (2/2);  total (30/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.83e-01

ACCGATCATCGACAGCGGTATGGTCACCACCGGAATCAGTACCGAGCGCAGGGCACCGAGGAACAGGAACACCACGACGATGACGATCAGAACCGCTTCGCCCAAGGTCTTGATCACTTCGTCGATCGAGGCCTGGATGAACCGCGTGGCGTCGTAGGCAATCGATACGTTCAGTGCCGAGGGCAGCTGGCTTTCCAGCTCAGGCATGATGCGCCGCACTTCCTTGATGACATCCAGCGGGTTGGCGGCCGGGGTGGCCTTGATGCCGATATAGACCGACGGGG  >  NC_002947/1047268‑1047551
                                                                                                                                                    |                                                                                                                                       
acCGATCATCGACAGCGGTATGGTCACCACCGGAATCAGTACCGAGCGCAGGGCACCGAGGAACAGGAACACCACGACGATGACGATCAGAACCGCTTCGCCCAAGGTCTTGATCACTTCGTCGATCGAGGCCTGGATGAACCGCGTGGc                                                                                                                                        >  2:370783/1‑150 (MQ=255)
    atcatcGACAGCGGTATGGTCACCACCGGAATCAGTACCGAGCGCAGGGCACCGAGGAACAGGAACACCACGACGATGACGATCAGAACCGCTTCGCCCAAGGTCTTGATCACTTCGTCGATCGAGGCCTGGATGAACCGCGTGGcgtcg                                                                                                                                    >  2:962404/1‑150 (MQ=255)
       atcGACAGCGGTATGGTCACCACCGGAATCAGTACCGAGCGCAGGGCACCGAGGAACAGGAACACCACGACGATGACGATCAGAACCGCTTCGCCCAAGGTCTTGATCACTTCGTCGATCGAGGCCTGGATGAACCGCGTGGCGTCGTAg                                                                                                                                 <  1:509668/150‑1 (MQ=255)
         cGACAGCGGTATGGTCACCACCGGAATCAGTACCGAGCGCAGGGCACCGAGGAACAGGAACACCACGACGATGACGATCAGAACCGCTTCGCCCAAGGTCTTGATCACTTCGTCGATCGAGGCCTGGATGAACCGCGTGGCGTCGTAGGc                                                                                                                               >  2:1153660/1‑150 (MQ=255)
          gACAGCGGTATGGTCACCACCGGAATCAGTACCGAGCGCAGGGCACCGAGGAACAGGAACACCACGACGATGACGATCAGAACCGCTTCGCCCAAGGTCTTGATCACTTGGTCGCTGGAGCCCTGCATGAACCGCTTGGCGTCGTAGGCa                                                                                                                              >  2:653970/1‑150 (MQ=255)
             aGCGGTATGGTCACCACCGGAATCAGTACCGAGCGCAGGGCACCGAGGAACAGGAACACCACGACGATGACGATCAGAACCGCTTCGCCCAAGGTCTTGATCACTTCGTCGATCGAGGCCTGGATGAACCGCGTGGCGTCGTAGGCAATc                                                                                                                           >  1:504848/1‑150 (MQ=255)
                ggTATGGTCACCACCGGAATCAGTACCGAGCGCAGGGCACCGAGGAACAGGAACACCACGACGATGACGATCAGAACCGCTTCGCCCAAGGTCTTGATCACTTCGTCGATCGAGGCCTGGATGAACCGCGTGGCGTCGTAGGCAATCGAt                                                                                                                        <  1:1153660/150‑1 (MQ=255)
                ggTATGGTCACCACCGGAATCAGTACCGAGCGCAGGGCACCGAGGAACAGGAACACCACGACGATGACGATCAGAACCGCTTCGCCCAAGGTCTTGATCACTTCGTCGATCGAGGCCTGGATGAACCGCGTGGCGTCGTAGGCAATCGAt                                                                                                                        <  1:177032/150‑1 (MQ=255)
                           caccGGAATCAGTACCGAGCGCAGGGCACCGAGGAACAGGCACACCACGACGATGACGATCAGAACCGCTTCGCCCAAGGTCTTGATCACTTCGTCGATCGAGGCCTGGATGAACCGCGTGGCGTCGTAGGCAATCGATACGTTCAGTGc                                                                                                             >  1:1224483/1‑150 (MQ=255)
                           caccGGAATCAGTACCGAGCGCAGGGCACCGAGGAACAGGAACACCACGACGATGACGATCAGAACCGCTTCGCCCAAGGTCTTGATCACTTCGTCGATCGAGGCCTGGATGAACCGCGTGGCGTCGTAGGCAATCGATACGTTCAGTGc                                                                                                             >  1:1014635/1‑150 (MQ=255)
                             ccGGAATCAGTACCGAGCGCAGGGCACCGAGGAACAGGAACACCACGACGATGACGATCAGAACCGCTTCGCCCAAGGTCTTGATCACTTCGTCGATCGAGGCCTGGATGAACCGCGTGGCGTCGTAGGCAATCGATACGTTCAGt                                                                                                               <  2:1259887/146‑1 (MQ=255)
                             ccGGAATCAGTACCGAGCGCAGGGCACCGAGGAACAGGAACACCACGACGATGACGATCAGAACCGCTTCGCCCAAGGTCTTGATCACTTCGTCGATCGAGGCCTGGATGAACCGCGTGGCGTCGTAGGCAATCGATACGTTCAGt                                                                                                               >  1:1259887/1‑146 (MQ=255)
                              cGGAATCAGTACCGAGCGCAGGGCACCGAGGAACAGGAACACCACGACGATGACGATCAGAACCGCTTCGCCCAAGGTCTTGATCACTTCGTCGATCGAGGCCTGGATGAACCGCGTGGCGTCGTAGGCAATCGATACGTTCAGTGCCGa                                                                                                          <  2:1014635/150‑1 (MQ=255)
                                         ccGAGCGCAGGGCACCGAGGAACAGGAACACCACGACGATGACGATCAGCACCGCTTCGCCCAAGGTCTTGATCACTTCGTCGATCGAGGCCTGGATGAACCGCGTGGCGTCGTAGGCAATCGATACGTTCAGTGCCGAGGGCAGCTGGc                                                                                               >  2:393615/1‑150 (MQ=255)
                                              cgcAGGGCACCGAGGAACAGGAACACCACGACGATGACGATCAGAACCGCTTCGCCCAAGGTCTTGATCACTTCGTCGATCGAGGCCTGGATGAACCGCGTGGCGTCGTAGGCAATCGAt                                                                                                                        <  2:1258754/120‑1 (MQ=255)
                                              cgcAGGGCACCGAGGAACAGGAACACCACGACGATGACGATCAGAACCGCTTCGCCCAAGGTCTTGATCACTTCGTCGATCGAGGCCTGGATGAACCGCGTGGCGTCGTAGGCAATCGAt                                                                                                                        >  1:1258754/1‑120 (MQ=255)
                                               gcAGGGCACCGAGGAACAGGAACACCACGACGATGACGATCAGAACCGCTTCGCCCAAGGTCTTGATCACTTCGTCGATCGAGGCCTGGATGAACCGCGTGGCGTCGTAGGCAATCGATACGTTCAGTGCCGAGGGCAGCTGGCTTTCCa                                                                                         <  1:644427/150‑1 (MQ=255)
                                                       ccGAGGAACAGGAACACCACGACGATGACGATCAGAACCGCTTCGCCCAAGGTCTTGATCACTTCGTCGATCGAGGCCTGGATGAACCGCGTGGCGTCGTAGGCAATCGATACGTTCAGTGCCGAGGGCAGCTGGCTTTCCAGCTCAGGc                                                                                 <  1:962404/150‑1 (MQ=255)
                                                        cGAGGAACAGGAACACCACGACGATGACGATCAGAACCGCTTCGCCCAAGGTCTTGATCACTTCGTCGATCGAGGCCTGGATGAACCGCGTGGCGTCGTAGGCAATCGATACGTTCAGTGCCGAGGGCAGCTGGCTTTCCAGCTCAGGCa                                                                                >  1:307589/1‑150 (MQ=255)
                                                        cGAGGAACAGGAACACCACGACGATGACGATCAGAACCGCTTCGCCCAAGGTCTTGATCACTTCGTCGATCGAGGCCTGGATGAACCGCGTGGCGTCGTAGGCAATCGATACGTTCAGTGCCGAGGGCAGCTGGCTTTCCAGCTCAGGCa                                                                                >  2:1206085/1‑150 (MQ=255)
                                                                   aacaCCACGACGATGACGATCAGAACCGCGTCGCGCAAGGTCTTGATCACTTCGTCGATCGAGGCCTGGATGAACCGCGTGGCGTCGTAGGCAATCGATACGTTCAGTGCCGAGGGCAGCTGGCTTTCCAGCTCAGGCATGATGcgccgc                                                                     >  2:197104/1‑150 (MQ=255)
                                                                           gacgatgacgatCAGAACCGCTTCGCCCAAGGTCTTGATCACTTCGTCGATCGAGGCCTGGATGAACCGCGTGGCGTCGTAGGCAATCGATACGTTCAGTGCCGAGGGCAGCTGGCTTTCCAGCTCAGGCATGATGCGCCGCACTTCCtt                                                             >  2:791746/1‑150 (MQ=255)
                                                                             cgatgacgatCAGAACCGCTTCGCCCAAGGTCTTGATCACTTCGTCGATCGAGGCCTGGATGAACCGCGTGGCGTCGTAGGCAATCGATACGTTCAGTGCCGAGGGCAGCTGGCTTTCCAGCTCAGGCATGATGCGCCGCACTTCCTtga                                                           <  2:307589/150‑1 (MQ=255)
                                                                              gatgacgatCAGAACCGCTTCGCCCAAGGTCTTGATCACTTCGTCGATCGAGGCCTGGATGAACCGCGTGGCGTCGTAGGCAATCGATACGTTCAGTGCCGAGGGCAGCTGGCTTTCCAGCTCAGGCATGATGCGCCGCACTTCCTtgat                                                          >  2:1079123/1‑150 (MQ=255)
                                                                              gatgacgatCAGAACCGCTTCGCCCAAGGTCTTGATCACTTCGTCGATCGAGGCCTGGATGAACCGCGTGGCGTCGTAGGCAATCGATACGTTCAGTGCCGAGGGCAGCTGGCTTTCCAGCTCAGGCATGATGCGCCGCACTTCCTtgat                                                          <  1:791746/150‑1 (MQ=255)
                                                                                   cggtCAGAACCGCTTCGCCCAAGGTCTTGATCACTTCGTCGATCGAGGCCTGGATGAACCGCGTGTCGTCGTAGGCAATCGATACGTTCAGTGCCGAGGGCAGCTGGCTTTcc                                                                                          >  1:1283026/4‑113 (MQ=255)
                                                                                   cggtCAGAACCGCTTCGCCCAAGGTCTTGATCACTTCGTCGATCGAGGCCTGGATGAACCGCGTGTCGTCGTAGGCAATCGATACGTTCAGTGCCGAGGGCAGCTGGCTTTcc                                                                                          >  1:1282952/4‑113 (MQ=255)
                                                                                   cgatCAGAACCGCTTCGCCCAAGGTCTTGATCACTTCGTCGATCGAGGCCTGGATGAACCGCGTGTCGTCGTAGGCAATCGATACGTTCAGTGCCGAGGGCAGCTGGCTTTcc                                                                                          <  2:1283026/113‑1 (MQ=255)
                                                                                   cgatCAGAACCGCTTCGCCCAAGGTCTTGATCACTTCGTCGATCGAGGCCTGGATGAACCGCGTGTCGTCGTAGGCAATCGATACGTTCAGTGCCGAGGGCAGCTGGCTTTcc                                                                                          <  2:1282952/113‑1 (MQ=255)
                                                                                         gAACCGCTTCGCCCAAGGTCTTGATCACTTCGTCGATCGAGGCCTGGATGAACCGCGTGGCGTCGTAGGCAATCGATACGTTCAGTGCCGAGGGCAGCTGGCTTTCCAGCTCAGGCATGATGCGCCGCACTTCCTTGATGACATCCAGCg                                               <  1:3694/150‑1 (MQ=255)
                                                                                                ttCGCCCAAGGTCTTGATCACTTCGTCGATCGAGGCCTGGATGAACCGCGTGGCGTCGTAGGCAATCGATACGTTCAGTGCCGAGGGCAGCTGGCTTTCCAGCTCAGGCATGATGCGCCGCACTTCCTTGATGACATCCAGCGGGTTggc                                        <  2:1224483/150‑1 (MQ=255)
                                                                                                   gCCCAAGGTCTTGATCACTTCGTCGATCGAGGCCTGGATGAACCGCGTGGCGTCGTAGGCAATCGATACGTTCAGTGCCGAGGGCAGCTGGCTTTCCAGCTCAGGCATGATGCGCCGCACTTCCTTGATGGCATCCAGCGGGTTggcggc                                     >  2:297294/1‑150 (MQ=255)
                                                                                                      cAAGGTCTTGATCACTTCGTCGATCGAGGCCTGGATGAACCGCGTGGCGTCGTAGGCAATCGATACGTTCAGTGCCGAGGGCAGCTGGCTTTCCAGCTCAGGCATGATGCGCCGCACTTCCTTGATGACATCCAGCGGGTTGGCGGCCgg                                  >  2:133770/1‑150 (MQ=255)
                                                                                                        aGGTCTTGATCACTTCGTCGATCGAGGCCTGGATGAACCGCGTGGCGTCGTAGGCAATCGATACGTTCAGTGCCGAGGGCAGCTGGCTTTCCAGCTCAGGCATGATGCGCCGCGCTTCCTTGATGACATCCAGCGGGTTGGCGGCCgggg                                <  2:504848/150‑1 (MQ=255)
                                                                                                        aGGTCTTGATCACTTCGTCGATCGAGGCCTGGATGAACCGCGTGGCGTCGTAGGCAATCGATACGTTCAGTGCCGAGGGCAGCTGGCTTTCCAGCTCAGGCATGATGCGCCGCACTTCCTTGATGACATCCAGCGGGTTGGCGGCCgggg                                <  1:1206085/150‑1 (MQ=255)
                                                                                                               gATCACTTCGTCGATCGAGGCCTGGATGAACCGCGTGGCGTCGTAGGCAATCGATACGTTCAGTGCCGAGGGCAGCTGGCTTTCCAGCTCAGGCATGATGCGCCGCACTTCCTTGATGACATCCAGCGGGTTGGCGGCCGGGGTg                              >  1:1158938/1‑145 (MQ=255)
                                                                                                               gATCACTTCGTCGATCGAGGCCTGGATGAACCGCGTGGCGTCGTAGGCAATCGATACGTTCAGTGCCGAGGGCAGCTGGCTTTCCAGCTCAGGCATGATGCGCCGCACTTCCTTGATGACATCCAGCGGGTTGGCGGCCGGGGTg                              <  2:1158938/145‑1 (MQ=255)
                                                                                                               gATCACTTCGTCGATCGAGGCCTGGATGAACCGCGTGGCGTCGTAGGCAATCGATACGTTCAGTGCCGAAGGCAGCTGGCTTTCCAGCTCAGCCATGATGCGCCTCACTTCCTTGATGACATCCAGCGGGTTGGCGGCCGGGGTGGCCtt                         <  1:197104/150‑1 (MQ=255)
                                                                                                                    cTTCGTCGATCGAGGCCTGGATGAACCGCGTGGCGTCGTAGGCAATCGATACGTTCAGTGCCGAGGGCAGCTGGCTTTCCAGCTCAGGCATGATGCGCCGCACTTCCTTGATGACATCCAGCGGGTTGGCGGCCGGGGTGGCCTTGATGc                    >  1:619190/1‑150 (MQ=255)
                                                                                                                      tcgtcgATCGAGGCCTGGATGAACCGCGTGGCGTCGTAGGCAATCGATACGTTCAGTGCCGAGGGCAGCTGGCTTTCCAGc                                                                                       >  1:1247435/1‑81 (MQ=255)
                                                                                                                      tcgtcgATCGAGGCCTGGATGAACCGCGTGGCGTCGTAGGCAATCGATACGTTCAGTGCCGAGGGCAGCTGGCTTTCCAGc                                                                                       <  2:1247435/81‑1 (MQ=255)
                                                                                                                       cgtcgATCGAGGCCTGGATGAACCGCGTGGCGTCGTAGGCAATCGATACGTTCAGTGCCGAGGGCAGCTGGCTTTCCAGCTCAGGCATGATGCGCCGCACTTCCTTGATGACATCCAGCGGGTTGGCGGCCGGGGTGGCCTTGATGCCGa                 >  2:1091198/1‑150 (MQ=255)
                                                                                                                        gtcgATCGAGGCCTGGATGAACCGCGTGGCGTCGTAGGCAATCGATACGTTCAGTGCCGAGGGCAGCTGGCTTTCCAGCTCAGGCATGATGCGCCGCACTTCCTTGATGACATCCAGCGGGTTGGCGGCCGGGGGGGCCTTGATGGCGat                >  1:134593/1‑150 (MQ=255)
                                                                                                                             tcgaGGCCTGGATGAACCGCGTGGCGTCGTAGGCAATCGATACGTTCAGTGCCGAGGGCAGCTGGCTTTCCAGCTCAGGCATGATGCGCCGCACTTCCTTGATGACATCCAGCGGGTTGGCGGCCGGGGTGGCCTTGATGCCGATATAGa           >  2:71045/1‑150 (MQ=255)
                                                                                                                              cgaGGCCTGGATGAACCGCGTGGCGTCGTAGGCAATCGATACGTTCAGTGCCGAGGGCAGCTGGCTTTCCAGCTCAGGCATGATGCGCCGCACTTCCTTGATGACATCCAGCGGGTTGGCGGCCGGGGTGGCCTTGATGCCGATATAGAc          <  1:1079123/150‑1 (MQ=255)
                                                                                                                              cgaGGCCTGGATGAACCGCGTGGCGTCGTAGGCAATCGATACGTTCAGTGCCGAGGGCAGCTGGCTTTCCAGCTCAGGCATGATGCGCCGCACTTCCTTGATGACATCCAGCGGGTTGGCGGCCGGGGTGGCCTTGATGCCGATATAGAc          <  1:71045/150‑1 (MQ=255)
                                                                                                                                    cTGGATGAACCGCGTGGCGTCGTAGGCAATCGATACGTTCAGTGc                                                                                                             <  1:79581/45‑1 (MQ=255)
                                                                                                                                    cTGGATGAACCGCGTGGCGTCGTAGGCAATCGATACGTTCAGTGCCGAGGGCAGCTGGCTTTCCAGCTCAGGCATGATGCGCCGCACTTCCTTGATGACATCCAGCGGGTTGGCGGCCGGGGTGGCCTTGATGCCGata               <  2:160192/139‑1 (MQ=255)
                                                                                                                                    cTGGATGAACCGCGTGGCGTCGTAGGCAATCGATACGTTCAGTGCCGAGGGCAGCTGGCTTTCCAGCCCAGGCATGATGCGCCGCACTTCCTTGATGACATCCAGCGGGTTGGCGGCCGGGGTGGCCTTGATGCCGata               >  1:160192/1‑139 (MQ=255)
                                                                                                                                    cTGGATGAACAGCGTGGCGTCGTAGGCAATCGATACGTTCAGTGc                                                                                                             >  2:79581/1‑45 (MQ=255)
                                                                                                                                     tGGATGAACCGCGTGGCGTCGTAGGCAATCGATACGTTCAGTGCCGAGGGCAGCTGGCTTTCCAGCTCAGGCATGATGCGCCGCACTTCCTTGATGACATCCAGCGGGTTGGCGGCCGGGGTGGCCTTGATGCCGATATAGACCGACggg   <  2:619190/150‑1 (MQ=255)
                                                                                                                                      ggATGAACCGCGTGGCGTCGTAGGCAATCGATACGTTCAGTGCCGAGGGCAGCTGGCTTTCCAGCTCAGGCATGATGCGCCGCACTTCCTTGATGACATCCAGCGGGTTGGCGGCCg                                   <  2:324739/117‑1 (MQ=255)
                                                                                                                                      ggATGAACCGCGTGGCGTCGTAGGCAATCGATACGTTCAGTGCCGAGGGCAGCTGGCTTTCCAGCTCAGGCATGATGCGCCGCACTTCCTTGATGACATCCAGCGGGTTGGCGGCCg                                   >  1:324739/1‑117 (MQ=255)
                                                                                                                                      ggATGAACCGCGTGGCGTCGTAGGCAATCGATACGTTCAGTGCCGAGGGCAGCTGGCTTTCCAGCTCAGGCATGATGCGCCGCACTTCCTTGATGACATCCAGCGGGTTGGCGGCCGGGGTGGCCTTGATGCCGATATAGACCGACgggg  <  2:1102603/150‑1 (MQ=255)
                                                                                                                                      ggATGAACCGCGTGGCGTCGTAGGCAATCGATACGTTCAGTGCCGAGGGCAGCTGGCTTTCCAGCTCAGGCATGATGCGCCGCACTTCCTTGATGACATCCAGCGGGTTGGCGGCCGGGGTGGCCTTGATGCCGATATAGACCGACgggg  >  2:728779/1‑150 (MQ=255)
                                                                                                                                        aTGAACCGCGTGGCGTCGTAGGCAATCGATACGTTCAGTGCCGAGGGCAGCTGGCTTTCCAGCTCAGGCATGATGCGCCGCACTTCCTTGATGACATCCAGCGGGTTGGCGGCCgg                                  <  2:765172/116‑1 (MQ=255)
                                                                                                                                        aTGAACCGCGTGGCGTCGTAGGCAATCGATACGTTCAGTGCCGAGGGCAGCTGGCTTTCCAGCTCAGGCATGATGCGCCGCACTTCCTTGATGACATCCAGCGGGTTGGCGGCCgg                                  >  1:765172/1‑116 (MQ=255)
                                                                                                                                                    |                                                                                                                                       
ACCGATCATCGACAGCGGTATGGTCACCACCGGAATCAGTACCGAGCGCAGGGCACCGAGGAACAGGAACACCACGACGATGACGATCAGAACCGCTTCGCCCAAGGTCTTGATCACTTCGTCGATCGAGGCCTGGATGAACCGCGTGGCGTCGTAGGCAATCGATACGTTCAGTGCCGAGGGCAGCTGGCTTTCCAGCTCAGGCATGATGCGCCGCACTTCCTTGATGACATCCAGCGGGTTGGCGGCCGGGGTGGCCTTGATGCCGATATAGACCGACGGGG  >  NC_002947/1047268‑1047551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑