Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 145,899 T→A 8.8% L378Q (CTG→CAG)  gltP → glutamate/aspartate‑proton DAACS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947145,8990TA 8.8% 124.3 / 3.9 46L378Q (CTG→CAG) gltPglutamate/aspartate‑proton DAACS transporter
Reads supporting (aligned to +/- strand):  ref base T (20/21);  new base A (2/2);  total (23/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.09e-01

TTGTACGGTATCGACCTGTCGTGGAGCCAGCAGCTGCTGCTGGTGCTGACCCTGATGGTCACTTCCAAAGGTATCGCGGGTGTGCCGGGCGTATCGTTCGTGGTACTGCTGGCCACCTTGGGCAGCGTGGGCATTCCGC‑TGGAAGGCCTGGCCTTCATCGCGGGCGTGGACCGCATCATGGACATGGCCCGTACTGCACTGAACGTTGTGGGCAACGCCTTGGCTGCATTGGTCATTGCTCGCTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGA  >  NC_002947/145751‑146045
                                                                                                                                                     |                                                                                                                                                  
ttGTACGGTATCGACCTGTCGTGGAGCCAGCAGCTGCTGCTGGTGCTGACGGTGATGGTCACTTCCAAAGGTATCGCGGGTGTGCCGGGCGTATCGTTCGTGGTACTGCTGGCCACCTTGGGCAGCGTGGGCATTCCGC‑TGGAAggcctg                                                                                                                                                   >  2:181505/1‑150 (MQ=255)
ttGTACGGTATCGACCTGTCGTGGAGCCAGCAGCTGCTGCTGGTGCTGACCCTGATGGTCACTTCCAAAGGTATCGCGGGTGTGCCGGGCGTATCGTTCGTGGTACTGCTGGCCACCTTGGGCAGCGTGGGCATTCCGC‑TGGAAggcctg                                                                                                                                                   <  2:1105765/150‑1 (MQ=255)
   tACGGTATCGACCTGTCGTGGAGCCAGCAGCTGCTGCTGGTGCTGACCCTGATGGTCACTTCCAAAGGTATCGCGGGTGTGCCGGGCGTATCGTTCGTGGTACTGCTGGCCACCTTGGGCAGCGTGGGCATTCCGC‑TGGAAggcctggcc                                                                                                                                                <  2:550348/150‑1 (MQ=255)
      ggTATCGACCTGTCGTGGAGCCAGCAGCTGCTGCTGGTGCTGACTGGGATGGTCACTTCCAAAGGTATCGCGGGTGTGCCGGGCGTATCGTTCGTGGTACTGCTGGCCACCTTGGGCAGCGTGGGCATTCCGC‑TGGAAGGCCTTGCCTTc                                                                                                                                             >  1:421185/1‑150 (MQ=255)
      ggTATCGACCTGTCGTGGAGCCAGCAGCTGCTGCTGGTGCTGACCCTGATGGTCACTTCCAAAGGTATCGCGGGTGTGCCGGGCGTATCGTTCGTGGTACTGCTGGCCACCTTGGGCAGCGTGGGCATTCCGC‑TGGAAGGCCTGGCCTTc                                                                                                                                             >  1:591688/1‑150 (MQ=255)
      ggTATCGACCTGTCGTGGAGCCAGCAGCTGCTGCTGGTGCTGACCCTGATGGTCACTTCCAAAGGTATCGCGGGTGTGCCGGGCGTATCGTTCGTGGTACTGCTGGCCACCTTGGGCAGCGTGGGCATTCCGC‑TGGAAGGCCTGGCCTTc                                                                                                                                             <  2:591688/150‑1 (MQ=255)
         aTCGACCTGTCGTGGAGCCAGCAGCTGCTGCTGGTGCTGACCCTGATGGTCACTTCCAAAGGTATCGCGGGTGTGGCGGGCGTATCGTTCGTGGTACTGCTGGCCACCTTGGGCAGCGTGGGCATTCCGC‑TGGAAGGCCTGGCCTTCATc                                                                                                                                          >  1:577582/1‑150 (MQ=255)
              ccTGTCGTGGAGCCAGCAGCTGCTGCTGGTGCTGACCCTGATGGTCACTTCCAAAGGTATCGCGGGTGTGCCGGGCGTATCGTTCGTGGTACTGCTGGCCACCTTGGGCAGCGTGGGCATTCCGCGATGAAGGCCAGGCCTTccagcgga                                                                                                                                      <  2:433743/150‑8 (MQ=255)
                tGTCGTGGAGCCAGCAGCTGCTGCTGGTGCTGACCCTGATGGTCACTTCCAAAGGTATCGCGGGTGTGCCGGGCGTATCGTTCGTGGTACTGCTGGCCACCTTGGGCAGCGTGGGCATTCCGC‑TGGAAGGCCTGGCCTTCATCGCGGGCg                                                                                                                                   >  1:1216347/1‑150 (MQ=255)
                          ccAGCAGCTGCTGCTGGTGCTGACCCTGATGGTCACTTCCAAAGGTATCGAGGGTATGCAGGGCGTATCGTTCGTGGTACTGCTGGACACCTTGGGCAGCGTGGGCATTCCGC‑TGGAAGGCCTGGACTTCATCGCGAGCGTGGACcacag                                                                                                                         >  2:568143/1‑149 (MQ=255)
                             gcagcTGCTGCTGGTGCTGACCCTGATGGTCACTTCCAAAGGTATCGCGGGTGTGCCGGGCGTATCGTTCGTGGTACTGCTGGCCACCTTGGGCAGCGTGGGCATTCAGC‑TGGAAGGCCGGGCCTTCATCTCGGGCGTGGACCGcatcat                                                                                                                      >  2:545185/1‑150 (MQ=255)
                                 ctgctgctgGTGCTGACCCTGATGGTCACTTCCAAAGGTATCGCGGGTGTGCCGGGCGTATCGTTCGTGGTACTGCTGGCCACCTTGGGCAGCGTGGGCATTCCGC‑TGGAAGGCCTGGCCTTCATCGCgg                                                                                                                                      <  1:1259451/130‑1 (MQ=255)
                                 ctgctgctgGTGCTGACCCTGATGGTCACTTCCAAAGGTATCGCGGGTGTGCCGGGCGTATCGTTCGTGGTACTGCTGGCCACCTTGGGCAGCGTGGGCATTCCGC‑TGGAAGGCCTGGCCTTCATCGCgg                                                                                                                                      >  2:1259451/1‑130 (MQ=255)
                                    ctgctgGTGCTGACCCTGATGGTCACTTCCAAAGGTATCGCGGGTGTGCCGGGCGTATCGTTCGTGGTACTGCTGGCCACCTTGGGCAGCGTGGGCATTCCGC‑TGGAAGGCCTGGCCTTCATCGCGGGCGTGGACCGCATCATGGACATg                                                                                                               <  2:502506/150‑1 (MQ=255)
                                            gCTGACCCTGATGGTCACTTCCAAAGGTATCGCGGGTGTGCCGGGCGTATCGTTCGTGGTACTGCTGGCCACCTTGGGCAGCGTGGGCATTCCGC‑TGGAAGGCCTGGCCTTCATCGCGGGCGTGGACCGCATCATGGACATGGc                                                                                                             >  1:8971/1‑144 (MQ=255)
                                            gCTGACCCTGATGGTCACTTCCAAAGGTATCGCGGGTGTGCCGGGCGTATCGTTCGTGGTACTGCTGGCCACCTTGGGCAGCGTGGGCATTCCGC‑TGGAAGGCCTGGCCTTCATCGCGGGCGTGGACCGCATCATGGACATGGc                                                                                                             <  2:8971/144‑1 (MQ=255)
                                            gCTGACCCTGATGGTCACTTCCAAAGGTATCGCGGGTGTGCCGGGCGTATCGTTCGTGGTACTGCTGGCCACCTTGGGCAGCGTGGGCATTCCGC‑TGGAAGGCCTGGCCTTCATCGCGGGCGTGGACCGCATCATGGACATGGCCCGTAc                                                                                                       <  2:606162/150‑1 (MQ=255)
                                                 cccTGATGGTCACTTCCAAAGGTATCGCGGGTGTGCCGGGCGTATCGTTCGTGGTACTGCTGGCCACCTTGGGCAGCGTGGGCATTCCGC‑TGGAAGGCCTGGCCTTCATCGCGGGCGTg                                                                                                                                 <  2:737560/119‑1 (MQ=255)
                                                 cccTGATGGTCACTTCCAAAGGTATCGCGGGTGTGCCGGGCGTATCGTTCGTGGTACTGCTGGCCACCTTGGGCAGCGTGGGCATTCCGC‑TGGAAGGCCTGGCCTTCATCGCGGGCGTg                                                                                                                                 >  1:737560/1‑119 (MQ=255)
                                                        ggTCACTTCCAAAGGTATCGCGGGTGTGCCGGGCGTATCGTTCGTGGTACTGCTGGCCACCTTGGGCAGCGTGGGCATTCCGC‑TGGAAGGCCTGGCCTTCATCGCGGGCGTGGACCGCATCATGGACATGGCCCGTACTGCACTGAACGt                                                                                           >  2:505156/1‑150 (MQ=255)
                                                          tCACTTCCAAAGGTATCGCGGGTGTGCCGGGCGTATCGTTCGTGGTACTGCTGGCCACCTTGGGCAGCGTGGGCATTCCGC‑TGGAAGGCCTGGCCTTCATCGCGGGCGTGGACCGCATCATGGACATGGCCCGTACTGCACTGAACGTtg                                                                                         <  2:1057154/150‑1 (MQ=255)
                                                            aCTTCCAAAGGTATCGCGGGTGTGCCGGGCGTATCGTTCGTGGTACTGCTGGCCACCTTGGGCAGCGTGGGCATTCCGC‑TGGAAGGCCTGGCCTTCATCGCGGGCGTGGACCGCATCATGGACATGGCCCGTACTGCACTGAACGTtgtg                                                                                       <  2:491971/150‑1 (MQ=255)
                                                                 cAAAGGTATCGCGGGTGTGCCGGGCGTATCGTTCGTGGTACTGCTGGCCACCTTGGGCAGCGTGGGCATTCCGC‑TGGAAGGCCTGGCCTTCATCGCGGGCGTGGACCGCATCATGGACATGGCCCGTACTGCACTGAACGTTGTgg                                                                                      >  2:17346/1‑146 (MQ=255)
                                                                 cAAAGGTATCGCGGGTGTGCCGGGCGTATCGTTCGTGGTACTGCTGGCCACCTTGGGCAGCGTGGGCATTCCGC‑TGGAAGGCCTGGCCTTCATCGCGGGCGTGGACCGCATCATGGACATGGCCCGTACTGCACTGAACGTTGTgg                                                                                      <  1:17346/146‑1 (MQ=255)
                                                                   aaGGTATCGCGGGTGTGCCGGGCGTATCGTTCGTGGTACTGCTGGCCACCTTGGGCAGCGTGGGCATTCCGC‑TGGAAGGCCTGGCCTTCATCGCGGGCGTGGACCGCATCATGGACATGGCCCGTACTGCACTGAACGTTGTGGGCAACg                                                                                >  1:866882/1‑150 (MQ=255)
                                                                    aGGTATCGCGGGTGTGCCGGGCGTATCGTTCGTGGTACTGCTGGCCACCTTGGGCAGCGTGGGCATTCCGC‑TGGAAGGCCTGGCCTTCATCGCGGGCGTGGACCGCATCATGGACATGGccc                                                                                                           <  1:1106794/122‑1 (MQ=255)
                                                                    aGGTATCGCGGGTGTGCCGGGCGTATCGTTCGTGGTACTGCTGGCCACCTTGGGCAGCGTGGGCATTCCGC‑TGGAAGGCCTGGCCTTCATCGCGGGCGTGGACCGCATCATGGACATGGccc                                                                                                           >  2:1106794/1‑122 (MQ=255)
                                                                               gtgtGCCGGGCGTATCGTTCGTGGTACTGCTGGCCACCTTGGGCAGCGTGGGCATTCCGC‑TGGAAGGCCTGGCCTTCATCGCGGGCGTGGACCGCATCATGGACATGGCCCGTACTGCACTGAACGTTGTGGGCAACGCCTTGGCTGCAt                                                                    <  2:1216347/150‑1 (MQ=255)
                                                                               gtgtGCCGGGCGTATCGTTCGTGGTACTGCTGGCCACCTTGGGCAGCGTGGGCATTCCGC‑TGGAAGGCCTGGCCTTCATCGCGGGCGTGGACCGCATCATGGACATGGCCCGTACTGCACTGAACGTTGTGGGCAACGCCTTGGCTGCAt                                                                    >  2:929116/1‑150 (MQ=255)
                                                                                       ggCGTATCGTTCGTGGTACTGCTGGCCACCTTGGGCAGCGTGGGCATTCCGC‑TGGAAGGCCTGGCCTTCATCGCGGGCGTGGACCGCATCATGGACATGGCCCGTACTGCACTGAACGTTGTGGGCAACGCCtt                                                                            >  1:356128/1‑134 (MQ=255)
                                                                                       ggCGTATCGTTCGTGGTACTGCTGGCCACCTTGGGCAGCGTGGGCATTCCGC‑TGGAAGGCCTGGCCTTCATCGCGGGCGTGGACCGCATCATGGACATGGCCCGTACTGCACTGAACGTTGTGGGCAACGCCtt                                                                            <  2:356128/134‑1 (MQ=255)
                                                                                                ttcgtGGTACTGCTGGCCACCTTGGGCAGCGTGGGCATTCCGC‑TGGAAGGCCTGGCCTTCATCGCGGGCGTGGACCGCATCATGGACATGGCCCGTACTGCACTGAACGTTGTGGGCAACGCCTTGGCTGCATTGGTCATTGCTCGCTgg                                                   >  1:771883/1‑150 (MQ=255)
                                                                                                   gtGGTACTGCTGGCCACCTTGGGCAGCGTGGGCATTCCGC‑TGGAAGGCCTGGCCTTCATCGCGGGCGTGGACCGCATCATGGACATGGCCCGTACTGCACTGAACGTTGTGGGCAACGCCTTGGCTGCATTGGTCATTGCTCGCTgggca                                                <  2:216781/150‑3 (MQ=255)
                                                                                                     ggTACTGCTGGCAACCTTGGGGCGCGTGAGCAGTCCGC‑TGGAAGGCGTGGCCTTCATCGCGGGCGTGGACCGAATCATGGACATGGTCCGTACTGCCCTGAACGTTGTGGGCAACGCCTTGGCTGCATTGGTCATTGCTCGCTGGGAAgg                                              <  2:421185/150‑1 (MQ=255)
                                                                                                          tgctgGCCACCTTGGGCAGCGTGGGCATTCCGC‑TGGAAGGCCTGGCCTTCATCGCGGGCGTGGACCGCATCATGGACATGGCCCGTACTGCACTGAACGTTGTGGGCAACGCCTTGGCTGCATTGGTCATTGCTCGCTGGGAAGGCATGt                                         >  2:293888/1‑150 (MQ=255)
                                                                                                           gctgGCCACCTTGGGCAGCGTGGGCATTCCGC‑TGGAAGGCCTGGCCTTCATCGCGGGCGTGGACCGCATCATGGACATGGCCCGTACTGCACTGAACGTTGTGGGCAACGCCTTGGCTGCATTGGTCATTGCTCGCTGGGAAGGCATGTa                                        <  2:577582/150‑1 (MQ=255)
                                                                                                                 cACCTTGGGCAGCGTGGGCATTCCGC‑TGGAAGGCCTGGCCTTCATCGCGGGCGTGGACCGCATCATGGACATGGCCCGTACTGCACTGaa                                                                                              >  2:400864/1‑90 (MQ=255)
                                                                                                                 cACCTTGGGCAGCGTGGGCATTCCGC‑TGGAAGGCCTGGCCTTCATCGCGGGCGTGGACCGCATCATGGACATGGCCCGTACTGCACTGaa                                                                                              <  1:400864/90‑1 (MQ=255)
                                                                                                                 cACCTTGGGCAGCGTGGGCATTCCGC‑TGGAAGGCCTGGCCTTCATCGCGGGCGTGGACCGCATCATGGACATGGCCCGTACTGCACTGAACGTTGTGGGCAACGCCTTGGCTGCATTGGTCAtt                                                            >  2:576738/1‑124 (MQ=255)
                                                                                                                 cACCTTGGGCAGCGTGGGCATTCCGC‑TGGAAGGCCTGGCCTTCATCGCGGGCGTGGACCGCATCATGGACATGGCCCGTACTGCACTGAACGTTGTGGGCAACGCCTTGGCTGCATTGGTCAtt                                                            <  1:576738/124‑1 (MQ=255)
                                                                                                                   ccTTGGGCAGCGTGGGCATTCCGC‑TGGAAGGCCTGGCCTTCATCGCGGGCGTGGACCGCATCATGGACATGGCCCGTACTGCACTGAACGTTGTGGGCAACGCCTTGGCTGCATTGGTCATTGCTCGCTGGGAAGGCATGTACGACGCgg                                <  2:866882/150‑1 (MQ=255)
                                                                                                                       gggCAGCGTGGGCATTCCGC‑TGGAAGGCCTGGCCTTCATCGCGGGCGTGGACCGCATCATGGACATGGCCCGTACTg                                                                                                     <  1:82907/77‑1 (MQ=255)
                                                                                                                       gggCAGCGTGGGCATTCCGC‑TGGAAGGCCTGGCCTTCATCGCGGGCGTGGACCGCATCATGGACATGGCCCGTACTg                                                                                                     >  2:82907/1‑77 (MQ=255)
                                                                                                                          cAGCGTGGGCATTCCGC‑TGGAAGGCCTGGCCTTCATCGCGGGCGTGGACCGCATCATGGACATGGCCCGTACTGCACTGAACGTTGTGGGCAACGCCTTGGCTGCATTGGTCATTGCTCGCTGGGAAGGCATGTACGACGCGGTCAAggg                         <  1:181505/150‑1 (MQ=255)
                                                                                                                               tGGGCATTCCGC‑TGGAAGGCCTGGCCTTCATCGCGGGCGTGGACCGCATCATGGACATGGCCCGTACTGCACTGAACGTTGTGGGCAACGCCTTGGCTGCATTGGTCATTg                                                           <  1:91978/111‑1 (MQ=255)
                                                                                                                               tGGGCATTCCGC‑TGGAAGGCCTGGCCTTCATCGCGGGCGTGGACCGCATCATGGACATGGCCCGTACTGCACTGAACGTTGTGGGCAACGCCTTGGCTGCATTGGTCATTg                                                           >  2:91978/1‑111 (MQ=255)
                                                                                                                                       ccgcgatgAAGGCCAGGCCTTCATCGCGGGCGTGGACCGCATCATGGACATGGCCCGTACTGCACTGAACGTTGTGGGCAACGCCTTGGCTGCATTGGTCATTGCTCGCTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGtacta             >  2:1118836/8‑150 (MQ=255)
                                                                                                                                           gatgAAGGCCAGGCCTTCATCGCGGGCGTGGACCGCATCATGGACATGGCCCGTACTGCACTGAACGTTGTGGGCAACGCCTTGGCTGCATTGGTCATTGCTCGCTGGGAAGGCATGTACGACGCGGTCAAg                           >  1:61718/4‑132 (MQ=255)
                                                                                                                                           gatgAAGGCCAGGCCTTCATCGCGGGCGTGGACCGCATCATGGACATGGCCCGTACTGCACTGAACGTTGTGGGCAACGCCTTGGCTGCATTGGTCATTGCTCGCTGGGAAGGCATGTACGACGCGGTCAAg                           <  2:61718/129‑1 (MQ=255)
                                                                                                                                                  gcctggcctTCATCGCGGGCGTGGACCGCATCATGGACATGGCCCGTACTGCACTGAACGTTGTGGGCAACGCCTTGGCTGCATTGGTCATTGCTCGCTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGa  >  2:122863/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
TTGTACGGTATCGACCTGTCGTGGAGCCAGCAGCTGCTGCTGGTGCTGACCCTGATGGTCACTTCCAAAGGTATCGCGGGTGTGCCGGGCGTATCGTTCGTGGTACTGCTGGCCACCTTGGGCAGCGTGGGCATTCCGC‑TGGAAGGCCTGGCCTTCATCGCGGGCGTGGACCGCATCATGGACATGGCCCGTACTGCACTGAACGTTGTGGGCAACGCCTTGGCTGCATTGGTCATTGCTCGCTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGA  >  NC_002947/145751‑146045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑