Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,129,437 A→T 8.1% A40A (GCT→GCA)  gcvP‑I ← glycine dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,129,4370AT 8.1% 145.2 / 4.7 51A40A (GCT→GCA) gcvP‑Iglycine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (22/24);  new base T (2/2);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.06e-01

TGGGTGTTGTAGTAGCCCTGGCCGATGTAGCTTTTGAACAGCTGGTTCTTGCCAGCGATGGCCTTGAGCGCAGCGAGCGCGTCGGCCTCGCTCTGGCCGTCGTGCGAGCCAAGCACGCTGGTACCTTTGATGCTGTCGGGGATGACCGCAGCGGTCATGGCGTCCAGCGAGTCGAAGCCCAGTGCAGTGAGCATGGCCTGTTCGTCAGCGGCGCGCGGGCCTATGTGACGGGCGATGAATTCGTTGGCGGTGCCGAGGTTGATGGTCATGGCGGGATTCCT  >  NC_002947/1129288‑1129568
                                                                                                                                                     |                                                                                                                                   
tgGGTGTTGTAGTAGCCCTGGCCGATGTAGCTTTTGAACAGCTGGTTCTTGCCAGCGATGGCCTTGAGCGCAGCGAGCGCGTCGGCCTCGCTCTGGCCGTCGTGCGAGCCAAGCACGCTGGTACCTTTGATGCTGTCGGGGATGACCGCa                                                                                                                                     <  1:868055/150‑1 (MQ=255)
tgGGTGTTGTAGTAGCCCTGGCCGATGTAGCTTTTGAACAGCTGGTTCTTGCCAGCGATGGCCTTGAGCGCAGCGAGCGCGTCGGCCTCGCTCTGGCCGTCGTGCGAGCCAAGCACGCTGGTACCTTTGATGCTGTCGGGGATGACCGCa                                                                                                                                     >  2:814394/1‑150 (MQ=255)
  ggTGTTGTAGTAGCCCTGGCCGATGTAGCTTTTGAACAGCTGGTTCTTGCCAGCGATGGCCTTGAGCGCAGCGAGCGCGTCGGCCTCGCTCTGGCCGTCGTGCGAGCCAAGCACGCTGGTACCTTTGATGCTGTCGGGGATGACCGCAGc                                                                                                                                   >  2:1096591/1‑150 (MQ=255)
   gtgtTGTAGTAGCCCTGGCCGATGTAGCTTTTGAACAGCTGGTTCTTGCCAGCGATGGCCTTGAGCGCAGCGAGCGCGTCGGCCTCGCTCTGGCCGTCGTGCGAGCCAAGCACGCTGGTACCTTTGATGCTGTCGGGGATGACCGCAGCg                                                                                                                                  >  2:85820/1‑150 (MQ=255)
       tgtAGTAGCCCTGGCCGATGTAGCTTTTGAACAGCTGGTTCTTGCCGGCGAGGGCCTTGAGCGCAGCGAGCGCGTCGGCCTCGCTCTGGCCGTCGTGCGAGCCAAGCACGCTGGTACCTTTGATGCTGTCGGGGATGACCGCAGCGGTCa                                                                                                                              >  2:1144654/1‑150 (MQ=255)
       tgtAGTAGCCCTGGCCGATGTAGCTTTTGAACAGCTGGTTCTTGCCAGCGATGGCCTTGAGCGCAGCGAGCGCGTCGGCCTCGCTCTGGCCGTCGTGCGAGCCAAGCACGCTGGTACCTTTGATGCTGTCGGGGATGACCGCAGCGGTCa                                                                                                                              <  1:306294/150‑1 (MQ=255)
         tagtagCCCTGGCCGATGTAGCTTTTGAACAGCTGGTTCTTGCCAGCGATGGCCTTGAGCGCAGCGAGCGCGTCGGCCTCGCTCTGGCCGTCGTGCGAGCCAAGCACGCTGGTACCTTTGATGCTGTCGGGGATGACCGCAGCGGTCATg                                                                                                                            >  2:898190/1‑150 (MQ=255)
           gtagCCCTGGCCGATGTAGCTTTTGAACAGCTGGTTCTTGCCAGCGATGGCCTTGAGCGCAGCGAGCGCGTCGGCCTCGCTCTGGCCGTCGTGCGAGCCAAGCACGCTGGTACCTTTGATGCTGTCGGGGATGACCGCAGCGGTCATGGc                                                                                                                          >  1:1205769/1‑150 (MQ=255)
           gtagCCCTGGCCGATGTAGCTTTTGAACAGCTGGTTCTTGCCAGCGATGGCCTTGAGCGCAGCGAGCGCGTCGGCCTCGCTCTGGCCGTCGTGCGAGCCAAGCACGCTGGTACCTTTGATGCTGTCGGGGATGACCGCAGCGGTCATGGc                                                                                                                          >  2:610619/1‑150 (MQ=255)
                 cTGGCCGATGTAGCTTTTGAACAGCTGGTTCTTGCCAGCGATGGCCTTGAGCGCAGCGAGCGCGTCGGCCTCGCTCTGGCCGTCGTGCGAGCCAAGCACGCTGGTACCTTTGATGCTGTCGGGGATGACCGCAGCGGTCATGGCGTCCAg                                                                                                                    >  2:675695/1‑150 (MQ=255)
                 cTGGCCGATGTAGCTTTTGAACAGCTGGTTCTTGCCAGCGATGGCCTTGAGCGCAGCGAGCGCGTCGGCCTCGCTCTGGCCGTCGTGCGAGCCAAGCACGCTGGTACCTTTGATGCTGTCGGGGATGACCGCAGCGGTCATGGCGTCCAg                                                                                                                    >  1:571447/1‑150 (MQ=255)
                  tGGCCGATGTAGCTTTTGAACAGCTGGTTCTTGCCAGCGATGGCCTTGAGCGCAGCGAGCGCGTCGGCCTCGCTCTGGCCGTCGTGCGAGCCAAGCACGCTGGTACCTTTGATGCTGTCGGGGATGACCGCAGCGGt                                                                                                                                >  2:991031/1‑137 (MQ=255)
                  tGGCCGATGTAGCTTTTGAACAGCTGGTTCTTGCCAGCGATGGCCTTGAGCGCAGCGAGCGCGTCGGCCTCGCTCTGGCCGTCGTGCGAGCCAAGCACGCTGGTACCTTTGATGCTGTCGGGGATGACCGCAGCGGt                                                                                                                                <  1:991031/137‑1 (MQ=255)
                  tGGCCGATGTAGCTTTTGAACAGCTGGTTCTTGCCAGCGATGGCCTTGAGCGCAGCGAGCGCGTCGGCCTCGCTCTGGCCGTCGTGCGAGCCAAGCACGCTGGTACCTTTGATGCTGTCGGGGATGACCGCAGCGGTCATGGCGTCCAGc                                                                                                                   <  1:748969/150‑1 (MQ=255)
                    gCCGATGTAGCTTTTGAACAGCTGGTTCTTGCCAGCGATGGCCTTGAGCGCAGCGAGCGCGTCGGCCTCGCTCTGGCCGTCGTGCGAGCCAAGCACGCTGGTACCTTTGATGCTGTCGGGGATGACCGCAGCGGTCATGGCGTCCAGCGa                                                                                                                 <  1:1096591/150‑1 (MQ=255)
                      cGATGTAGCTTTTGAACAGCTGGTTCTTGCCAGCGATGGCCTTGAGCGCAGCGAGCGCGTCGGCCTCGCTCTGGCCGTCGTGCGAGCCAAGCACGCTGGTACCTTTGATGCTGTCGGGGATGACCGCTGCgg                                                                                                                                 <  1:934160/132‑1 (MQ=255)
                      cGATGTAGCTTTTGAACAGCTGGTTCTTGCCAGCGATGGCCTTGAGCGCAGCGAGCGCGTCGGCCTCGCTCTGGCCGTCGTGCGAGCCAAGCACGCTGGTACCTTTGATGCTGTCGGGGATGACCGCTGCgg                                                                                                                                 >  2:934160/1‑132 (MQ=255)
                          gTAGCTTTTGAACAGCTGGTTCTTGCCAGCGATGGCCTTGAGCGCAGCGAGCGCGTCGGCCTCGCTCTGGCCGTCGTGCGAGCCAAGCACGCTGGTACCTTTGATGCTGTCGGGGATGACCGCAGCGGTCATGGCGTCCAGCGAGTCGaa                                                                                                           <  2:489490/150‑1 (MQ=255)
                               ttttGAACAGCTGGTTCTTGCCAGCGATGGCCTTGAGCGCAGCGAGCGCGTCGGCCTCGCTCTGGCCGTCGTGCGAGCCAAGCACGCTGGTACCTTTGATGCTGTCGGGGATGACCGCAGCGGTCATGGCGTCCAGCGAGTCGAAGCCca                                                                                                      <  2:404841/150‑1 (MQ=255)
                               ttttGAACAGCTGGTTCTTGCCAGCGATGGCCTTGAGCGCAGCGAGCGCGTCGGCCTCGCTCTGGCCGTCGTGCGAGCCAAGCACGCTGGTACCTTTGATGCTGTCGGGGATGACCGCAGCGGTCATGGCGTCCAGCGAGTCGAAGCCca                                                                                                      >  2:1218569/1‑150 (MQ=255)
                               ttttGAACAGCTGGTTCTTGCCAGCGATGGCCTTGAGCGCAGCGAGCGCGTCGGCCTCGCTCTGGCCGTCCTGCGAGCCAAGCGGGCTGGTACCGTGGATGCTGTCGGGGATGACCGCAGCGGTCATGGCGTCCAGCGAGTCGAAGCCca                                                                                                      >  1:1253771/1‑150 (MQ=255)
                                    aaCAGCTGGTTCTTGCCAGCGATGGCCTTGAGCGCAGCGAGCGCGTCGGCCTCGCTCTGGCCGTCGTGCGAGCCAAGCACGCTGGTACCTTTGATGCTGTCGGGGATGACCGCAGCGGTCATGGCGTCCAGCGAGTCGAAGCCcagtgca                                                                                                 <  2:197720/150‑1 (MQ=255)
                                       aGCTGGTTCTTGCCAGCGATGGCCTTGAGCGCAGCGAGCGCGTCGGCCTCGCTCTGGCCGTCGTGCGAGCCAAGCACGCTGGTACCTTTGATGCTGTCGGGGATGACCGCAGCGGTCATGGCGTCCAGCGAGTCGAAGCCcagtgcagtg                                                                                              >  1:1150528/1‑150 (MQ=255)
                                        gCTGGTTCTTGCCAGCGATGGCCTTGAGCGCAGCGAGCGCGTCGGCCTCGCTCTGGCCGTCGTGCGAGCCAAGCACGCTGGTACCTTTGATGCTGTCGGGGATGACCGCAGCGGTCATGGCGTCCAGCGAGTCGAAGCCCAGTGCAGTGa                                                                                             >  1:686561/1‑150 (MQ=255)
                                         cTGGTTCTTGCCAGCGATGGCCTTGAGCGCAGCGAGCGCGTCGGCCTCGCTCTGGCCGTCGTGCGAGCCAAGCACGCTGGTACCTTTGATGCTGTCGGGGATGACCGCAGCGGTCATGGCGTCCAGCGAGTCGAAGCCCAGTGCAGTGAg                                                                                            >  2:934020/1‑150 (MQ=255)
                                              tCTTGCCAGCGATGGCCTTGAGCGCAGCGAGCGCGTCGGCCTCGCTCTGGCCGTCGTGCGAGCCAAGCACGCTGGTACCTTTGATGCTGTCGGGGATGACCGCAGCGGTCATGGCGTCCAGCGAGTCGAAGCCCAGTGCAGTGAGCATgg                                                                                       <  1:675695/150‑1 (MQ=255)
                                               cTTGCCAGCGATGGCCTTGAGCGCAGCGAGCGCGTCGGCCTCGCTCTGGCCGTCGTGCGAGCCAAGCACGCTGGTACCTTTGATGCTGTCGGGGATGACCGCAGCGGTCATGGCGTCCAGCGAGTCGAAGCCCAGTGCAGTGAGCATGGc                                                                                      <  2:686561/150‑1 (MQ=255)
                                               cTTGCCAGCGATGGCCTTGAGCGCAGCGAGCGCGTCGGCCTCGCTCTGGCCGTCGTGCGAGCCAAGCACGCTGGTACCTTTGATGCTGTCGGGGATGACCGCAGCGGTCATGGCGTCCAGCGAGTCGAAGCCCAGTGCAGTGAGCATGGc                                                                                      <  2:571447/150‑1 (MQ=255)
                                                 tGCCAGCGATGGCCTTGAGCGCAGCGAGCGCGTCGGCCTCGCTCTGGCCGTCGTGCGAGCCAAGCACGCTGGTACCTTTGATGCTGTCGGGGATGACCGCAGCGGTCATGGCGTCCAGCGAGTCGAAGCCCAGTGCAGTGAGCATGGCCt                                                                                    <  2:1019522/150‑1 (MQ=255)
                                                   ccAGCGATGGCCTTGAGCGCAGCGAGCGCGTCGGCCTCGCTCTGGCCGTCGTGCGAGCCAAGCACGCTGGTACCTTTGATGCTGTCGGGGATGACCGCGGCGTTCATGGCGTCCAGCGAGTCGAAGCCCAGTGCAGTGAGCATGGCCTGt                                                                                  >  2:999083/1‑150 (MQ=255)
                                                          tGGCCTTGAGCGCAGCGAGCGCGTCGGCCTCGCTCTGGCCGTCGTGCGAGCCAAGCACGCTGGTACCTTTGATGCTGTCGGGGATGACCGCATCGGTCATGGCGTCCAGCGAGTCGAAGCCCAGTGCAGTGAGCATGGCCTGTTCGTCAg                                                                           <  2:176157/150‑1 (MQ=255)
                                                            gCCTTGAGCGCAGCGAGCGCGTCGGCCTCGCTCTGGCCGTCGTGCGAGCCAAGCACGCTGGTACCTTTGATGCTGTCGGGGATGACCGCAGCGGTCATGGCGTCCAGCGAGTCGAAGCCCAGTGCAGTGAGCATGGCCTGTTCGTCAgcg                                                                         <  2:55309/150‑1 (MQ=255)
                                                              cTTGAGCGCAGCGAGCGCGTCGGCCTCGCTCTGGCCGTCGTGCGAGCCAAGCACGCTGGTACCTTTGATGCTGTCGGGGATGACCGCAGCGGTCATGGCGTCCAGCGAGTCGAAGCCCAGTGCAGTGAGCATGGCCTGTTCGTCAgcggc                                                                       >  1:702449/1‑150 (MQ=255)
                                                                 gAGCGCAGCGAGCGCGTCGGCCTCGCTCTGGCCGTCGTGCGAGCCAAGCACGCTGGTACCTTTGATGCTGTCGGGGATGACCGCAGCGGTCATGGCGTCCAGCGAGTCGAAGCCCAGTGCAGTGAGCATGGCCTGTTc                                                                                <  2:906492/138‑1 (MQ=255)
                                                                 gAGCGCAGCGAGCGCGTCGGCCTCGCTCTGGCCGTCGTGCGAGCCAAGCACGCTGGTACCTTTGATGCTGTCGGGGATGACCGCAGCGGTCATGGCGTCCAGCGAGTCGAAGCCCAGTGCAGTGAGCATGGCCTGTTc                                                                                >  1:906492/1‑138 (MQ=255)
                                                                    cgcAGCGAGCGCGTCGGCCTCGCTCTGGCCGTCGTGCGAGCCAAGCACGCTGGTACCTTTGATGCTGTCGGGGATGACCGCAGCGGTCATGGCGTCCAGCGAGTCGAAGCCCAGTGCAGTGAGCATGGCCTGTTCGTCAGCGGCGCGCgg                                                                 <  1:893993/150‑1 (MQ=255)
                                                                                   ggCCTCGCTCTGGCCGTCGTGCGAGCCAAGCACGCTGGTACCTTTGATGCTGTCGGGGATGACCGCAGCGGTCATGGCGTCCAGCGAGTCGAAGCCCAGTGCAGTGAGCATGGCCTGTTCGTCAGCGGCGCGCGGGCCTATGTGACGGGc                                                  <  1:814394/150‑1 (MQ=255)
                                                                                     ccTCGCTCTGGCCGTCGTGCGAGCCAAGCACGCTGGTACCTTTGATGCTGTCGGGGATGACCGCAGCGGTCATGGCGTCCAGCGAGTCGAAGCCCAGTGCAGTGAGCATGGCCTGTTCGTCAGCGGCGCGCGGGCCTATGTGACGGGCGa                                                <  1:934020/150‑1 (MQ=255)
                                                                                      cTCGCTCTGGCCGTCGTGCGAGTCAAGCACGCTGGTACCTTTGATGCTGTCGGGGATGACCGCAGCGGTCATGGCGTCCAGCGAGTCGAAGCCCAGTGCAGTGAGCATGGCCTGTTCGTCAGCGGCGCGCGGGCCTATGTGACGGGCGAt                                               <  2:1205769/150‑1 (MQ=255)
                                                                                       tCGCTCTGGCTGTCGTGCGAGCCAAGCACGCTGGTACCTTTGATGCTGTCGGGGATGACCGCAGCGGTCATGGCGTCCAGCGAGTCGAAGCCCAGTGCAGTGAGCATGGCCTGTTCGTCAGCGGCGCGCGGGCCTATGTGACGGGCGATg                                              >  1:1146274/1‑150 (MQ=255)
                                                                                            ctGGCTGTCGTGCGAGCCAAGCACGCTGGTACCTTTGATGCTGTCGGGGATGACCGCAGCGGTCATGGCGTCCAGCGAGTCGAAGCCCAGTGCAGTGAGCATGGCCTGTTCGTCAGCGGCGCGCGGGCCTATGTGACGGGCGATGAATTc                                         <  2:1146274/150‑1 (MQ=255)
                                                                                             tGGCCGTCGTGCGAGCCAAGCACGCTGGTACCTTTGATGCTGTCGGGGATGACCGCAGCGGTCATGGCGTCCAGCGAGTCGAAGCCCAGTGCAGTGAGCATGGCCTGTTCGTCAGCGGCGCGCGGGCCTATGTGACGGGCGATGAATTCg                                        >  2:973358/1‑150 (MQ=255)
                                                                                                 cgtcgtGCGAGCCAAGCACGCTGGTACCTTTGATGCTGTCGGGGATGACCGCAGCGGTCATGGCGTCCAGCGAGTCGAAGCCCAGTGCAGTGAGCATGGCCTGTTCGTCAGCGGCGCGCGGGCCTATGTGACGGGCGATGAATTCGTTgg                                    >  2:777616/1‑150 (MQ=255)
                                                                                                             cAAGCACGCTGGTACCTTTGATGCTGTCGGGGATGACCGCAGCGGTCATGGCGTCCAGCGAGTCGAAGCCCAGTGCAGTGAGCATGGCCTGTTCGTCAGCGGCGCGCGGGCCTATGTGACGGGCGATGAATTCGTTGGCGGTGCCGAGGt                        >  1:278598/1‑150 (MQ=255)
                                                                                                                 cACGCTGGTACCTTTGATGCTGTCGGGGATGACCGCAGCGGTCATGGCGTCCAGCGAGTCGAAGCCCAGTGCAGTGAGCATGGCCTGTTCGTCAGCGGCGCGCGGGCCTATGTGACGGGCGATGAATTCGTTGGCGGTGCCGAGGTTTAt                    <  1:1144654/150‑1 (MQ=255)
                                                                                                                 cACGCTGGTACCTTTGATGCTGTCGGGGATGACCGCAGCGGTCATGGCGTCCAGCGAGTCGAAGCCCAGTGCAGTGAGCATGGCCTGTTCGTCAGCGGCGCGCGGGCCTATGTGACGGGCGATGAATTCGTTGGCGGTGCCGAGGTTGAt                    <  2:1253771/150‑1 (MQ=255)
                                                                                                                           ccTTTGATGCTGTCGGGGATGACCGCAGCGGTCATGGCGTCCAGCGAGTCGAAGCCCAGTGCAGTGAGCATg                                                                                        <  1:827978/72‑1 (MQ=255)
                                                                                                                           ccTTTGATGCTGTCGGGGATGACCGCAGCGGTCATGGCGTCCAGCGAGTCGAAGCCCAGTGCAGTGAGCATg                                                                                        >  2:827978/1‑72 (MQ=255)
                                                                                                                           ccTTTGATGCTGTCGGGGATGACCGCAGCGGTCATGGCGTCCAGCGAGTCGAAGCCCAGTGCAGTGAGCATGGCCTGTTCGTCAGCGGCGCGCGGGCCTATGTGACGGGCGATGAATTCGTTGGCGGTGCCGAGGTTGATGGTCATGGCg          <  1:777616/150‑1 (MQ=255)
                                                                                                                            cTTTGATGCTGTCGGGGATGACCGCAGCGGTCATGGCGTCCAGCGAGTCGAAGCCCAGTGCAGTGAGCATGGCCTGTTCGTCAGCGGCGCGCGGGCCTATGTGACGGGCGATGAATTCGTTGGCGGTGCCGAGGTTGATGGTCATGGCgg         >  1:1059700/1‑150 (MQ=255)
                                                                                                                                   gCTGTCGGGGATGACCGCAGCGGTCATGGCGTCCAGCGAGTCGAAGCCCCGTGCAGTGAGCCTGGCCTGTTCGTCAGCGGCGCGCGGGCCTATGTCACGGGCGATGAATTCGTTGGCGGTGCCGAGGTTGATGGTCATGGCGGGATTCCt  <  1:610619/150‑1 (MQ=255)
                                                                                                                                            caTGACCGCTGCGGTCATGGCGTCCAGCGAGTCGAAGCCCAGTGCAGTGAGCATGGCCTGTTCGTCAGCGGCGCGCGGGCCTATGTGACGGGCGATGAATTCGTTGGCGGTGCCGAgg                         >  2:1027971/2‑118 (MQ=255)
                                                                                                                                            caTGACCGCTGCGGTCATGGCGTCCAGCGAGTCGAAGCCCAGTGCAGTGAGCATGGCCTGTTCGTCAGCGGCGCGCGGGCCTATGTGACGGGCGATGAATTCGTTGGCGGTGCCGAgg                         <  1:1027971/117‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                   
TGGGTGTTGTAGTAGCCCTGGCCGATGTAGCTTTTGAACAGCTGGTTCTTGCCAGCGATGGCCTTGAGCGCAGCGAGCGCGTCGGCCTCGCTCTGGCCGTCGTGCGAGCCAAGCACGCTGGTACCTTTGATGCTGTCGGGGATGACCGCAGCGGTCATGGCGTCCAGCGAGTCGAAGCCCAGTGCAGTGAGCATGGCCTGTTCGTCAGCGGCGCGCGGGCCTATGTGACGGGCGATGAATTCGTTGGCGGTGCCGAGGTTGATGGTCATGGCGGGATTCCT  >  NC_002947/1129288‑1129568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑