Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,522,170 G→T 8.1% P12P (CCG→CCT)  ftsW → lipid II flippase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,522,1700GT 8.1% 153.3 / 2.7 49P12P (CCG→CCT) ftsWlipid II flippase
Reads supporting (aligned to +/- strand):  ref base G (26/19);  new base T (2/2);  total (28/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

GCCCAGGCCGGCGATGCCGTGCTGCTGTCGCCAGCATGCGCCAGCCTCGACATGTTCAAGAACTTCGAGGAACGGGGACGCCTGTTCGCCCAGGCAGCGGGAGGTCTTGCATGATCTTCGGCATCTTCAAACCCTACCCGTCGCCGCTGATCAGCGGCCGTGGCATCGACCTGGACTTCCCGATGCTCGCCGGTTGCCTGGCCTTGCTGGGCCTGGGCCTGGTGATGATCACTTCGGCCTCCTCGGAAGTGGCTGCGGTACAGTCCGGCAACCCGCTGTATCACATGTTTCGC  >  NC_002947/1522025‑1522317
                                                                                                                                                 |                                                                                                                                                   
gCCCAGGCCGGCGATGCCGTGCTGCTGTCGCCAGCATGCGCCAGCCTCGACATGTTCAAGAACTTCGAGGAACGGGGACGCCTGTTCGCCCAGGCAGCGGGAGGTCTTGCATGATCTTCGGCATCTTCAAACCCTACCCGTCGCCGCTGa                                                                                                                                                 >  2:158549/1‑150 (MQ=255)
gCCCAGGCCGGCGATGCCGTGCTGCTGTCGCCAGCATGCGCCAGCCTCGACATGTTCAAGAACTTCGAGGAACGGGGACGCCTGTTCGCCCAGGCAGCGGGAGGTCTTGCATGATCTTCGGCATCTTCAAACCCTACCCGTCGCCGCTGa                                                                                                                                                 >  2:798643/1‑150 (MQ=255)
     gccccgcgcTGCCGTGCTGGTGTCGCCATTCTCCGCCAGGCTCGACATGTTCAAGAACTTCTAGGAACGGGGACGCCTGTTCGCCCAGGCAGGGGGAGGGCTTGCATGATGTTCGGCATCTTCAAACCCTGCCCGTCGCAGCTGATCAGc                                                                                                                                            <  2:525673/145‑1 (MQ=255)
           cGATGCCGTTCTGCTGTCGCCAGCATTCGCCAGCCTCGACATGTTCAAGAACTTCGAGGAACGGGGACGCCTGTTCGCCCAGGCAGCGGGAGGTCTTGCATGATCTTCGGCATCTTCAAACCCTACCCGTCGCCGCTGATCAGCGGcgac                                                                                                                                      <  1:637162/150‑4 (MQ=255)
            gATGCCGTGCTGCTGTCGCCAGCATGCGCCAGCCTCGACATGTTCAAGAACTTCGAGGAACGGGGACGCCTGTTCGCCCAGGCAGCGGGAGGTCTTGCATGATCTTCGGCATCTTCAAACCCTACCCGTCGCCGCTGATCAGCGGCCGTg                                                                                                                                     <  1:1143381/150‑1 (MQ=255)
                   tgctgcTGTCGCCAGCATGCGCCAGCCTCGACATGTTCAAGAACTTCGAGGAACGGGGACGCCTGTTCGCCCAGGCAGCGGGAGGTCTTGCATGATCTTCGGCATCTTCAAACCCTACCCGTCGCCGCTGATCAGCGGCCGTGGCATCGa                                                                                                                              <  2:1144119/150‑1 (MQ=255)
                             gCCAGCATGCGCCAGCCTCGACATGTTCAAGAACTTCGAGGAACGGGGACGCCTGTTCGCCCAGGCAGCGGGAGGTCTTGCATGATCTTCGGCATCTTCAAACCCTACCCGTCGCCGCTGATCAGCGGCCGTGGCATCGACCTGGACTTc                                                                                                                    >  1:943950/1‑150 (MQ=255)
                              ccAGCATGCGCCAGCCTCGACATGTTCAAGAACTTCGAGGAACGGGGACGCCTGTTCGCCCAGGCAGCGGGAGGTCTTGCATGATCTTCGGCATCTTCAAACCCTACCCGTCGCCGCt                                                                                                                                                   <  2:284672/118‑1 (MQ=255)
                              ccAGCATGCGCCAGCCTCGACATGTTCAAGAACTTCGAGGAACGGGGACGCCTGTTCGCCCAGGCAGCGGGAGGTCTTGCATGATCTTCGGCATCTTCAAACCCTACCCGTCGCCGCt                                                                                                                                                   >  1:284672/1‑118 (MQ=255)
                                  cATGCGCCAGCCTCGACATGTTCAAGAACTTCGAGGAACGGGGACGCCTGTTCGCCCAGGCAGCGGGAGGTCTTGCATGATCTTCGGCATCTTCAAACCCTACCCGTCGCCGCTGATCAGCGGCCGTGGCATCGACCTGGACTTCCCGAt                                                                                                               >  2:1194625/1‑150 (MQ=255)
                                                   aTGTTCAAGAACTTCGAGGAACGGGGACGCCTGTTCGCCCAGGCAGCGGGAGGTCTTGCATGATCTTCGGCATCTTCAAACCCTACCCGTCGCCGCTGATCAGCGGCCGTGGCATCGACCTGGACTTCCCGATGCTCGCCGGTTGc                                                                                                  >  1:414084/1‑146 (MQ=255)
                                                   aTGTTCAAGAACTTCGAGGAACGGGGACGCCTGTTCGCCCAGGCAGCGGGAGGTCTTGCATGATCTTCGGCATCTTCAAACCCTACCCGTCGCCGCTGATCAGCGGCCGTGGCATCGACCTGGACTTCCCGATGCTCGCCGGTTGc                                                                                                  <  2:414084/146‑1 (MQ=255)
                                                         aaGAACTTCTTGGAACTTTGTCGCCTGTTCGCCCAGGCAGCGGGAGGTCTTGCATGATCTTCGGCATCTTCAAACCCTACCCGTCGCCGCTGATCAGCGGCCGTGGCATCGACCTGGACTTCCCGATGCTCGCCGGTTGCCTGGCCTTGc                                                                                        >  2:258150/1‑150 (MQ=255)
                                                          aGAACTTCGAGGAACGGGGACGCCTGTTCGCCCAGGCAGCGGGAGGTCTTGCATGATCTTCGGCATCTTCAAACCCTACCCGTCGCCGCTGATCAGCGGCCGTGGCATCGACCTGGACTTCCCGATGCTCGCCGGTTGCCTGGCCTTGct                                                                                       >  1:227280/1‑150 (MQ=255)
                                                           gAACTTCGAGGAACGGGGACGCCTGTTCGCCCAGGCAGCGGGAGGTCTTGCATGATCTTCGGCATCTTCAAACCCTACCCGTCGCCGCTGATCAGCGGCCGTGGCATCGACCTGGACTTCCCGATGCTCGCCGGTTGCCTGGCCTTGctg                                                                                      >  2:619441/1‑150 (MQ=255)
                                                               ttCGAGGAACGGGGACGCCTGTTCGCCCAGGCAGCGGGAGGTCTTGCATGATCTTCGGCATCTTCAAACCCTACCGGTCGCCGCTGATCAGCGGCCGTGGCATCGACCTGGACTTCCCGATGCTCGCCGGTTGCCTGGCCTTGctgggcc                                                                                  <  1:460253/150‑1 (MQ=255)
                                                                      aaCGGGGACGCCTGTTCGTCTAGGCAGCGGGAGGTCTTGCATGATTTTCGGCATCTTCAAACCCTACCCGTCGCCGCTGATCAGCGGCCGTGGCATCGACCTGGACTTCCCGATGCTCGCCGGTTGCCTGGCCTTGCTgggcctgggcct                                                                           <  2:539995/150‑1 (MQ=255)
                                                                           ggACGCCTGTTCGCCCAGGCAGCGGGAGGTCTTGCATGATCTTCGGCATCTTCAAACCCTACCCGTCGCCTCTGATCAGCGGCCGTGGCATCGACCTgg                                                                                                                         >  1:728014/1‑99 (MQ=255)
                                                                           ggACGCCTGTTCGCCCAGGCAGCGGGAGGTCTTGCATGATCTTCGGCATCTTCAAACCCTACCCGTCGCCTCTGATCAGCGGCCGTGGCATCGACCTgg                                                                                                                         >  1:728059/1‑99 (MQ=255)
                                                                           ggACGCCTGTTCGCCCAGGCAGCGGGAGGTCTTGCATGATCTTCGGCATCTTCAAACCCTACCCGTCGCCTCTGATCAGCGGCCGTGGCATCGACCTgg                                                                                                                         <  2:728014/99‑1 (MQ=255)
                                                                           ggACGCCTGTTCGCCCAGGCAGCGGGAGGTCTTGCATGATCTTCGGCATCTTCAAACCCTACCCGTCGCCTCTGATCAGCGGCCGTGGCATCGACCTgg                                                                                                                         <  2:728059/99‑1 (MQ=255)
                                                                           ggACGCCTGTTCGCCCAGGCAGCGGGAGCGCTTGCATGATCTTCGGCATCTTCAAACCCTACCCGTCGCCGCTGATCAGCGGCCGTGGCATCGACCTGGACTTCCCGATGCTCGCCGGTTGCCTGGCCTTGCTGGGCCTGGGCCTGGtga                                                                      <  1:966139/150‑1 (MQ=255)
                                                                             aCGCCTGTTCGCCCAGGCAGCGGGAGGTCTTGCATGATCTTCGGCATCTTCAAACCCTACCCGTCGCCGCTGATCAGCGGCCGTGGCATCGACCTGGACTTCCCGATGCTCGCCGGTTGCCTGGCCTTGCTGGGCCTGGGCCTGGtgatg                                                                    >  2:1200909/1‑150 (MQ=255)
                                                                              cGCCTGTTCGCCCAGGCAGCGGGAGGTCTTGCATGATCTTCGGCATCTTCAAACCCTACCCGTCGCCGCTGATCAGCGGCCGTGGCATCGACCTGGACTTCCCGATGCTCGCCGGTTGCCTGGCCTTGCTGGGCCTGGGCCTGGtgatga                                                                   <  1:258150/150‑1 (MQ=255)
                                                                                    ttCGCCCAGGCAGCGGGAGGTCTTGCATGATCTTCGGCATCTTCAAACCCTACCCGTCGCCGCTGATCAGCGGCCGTGGCATCGACCTGGACTTCCCGATGCTCGCCGGTTGCCTGGCCTTGCTGGGCCTGGGCCTGGTGATGATCACtt                                                             >  2:815228/1‑150 (MQ=255)
                                                                                        cccAGGCAGCGGGAGGTCTTGCATGATCTTCGGCATCTTCAAACCCTACCCGTCGCCGCTGATCAGCGGCCGCGCCCCCGACCTGGAATTCATGATGCTCGCCGGTTTCCTGGCCTTGCTGGGCCTGGGCCTGGTGATGATCACTTCGGc                                                         <  1:798643/150‑1 (MQ=255)
                                                                                                  gggAGGTCTTGCATGATCTTCGGCATCTTCAAACCCTACCCGTCGCCGCTGATCAGCGGCCGTGGCATCGACCTGGACTTCCCGATGCTCGCCGGTTGCCTGGCCTTGCTGGGCCTGGGCCTGGTGATGATCACTTCGGCCTCCTCGGaa                                               >  2:406631/1‑150 (MQ=255)
                                                                                                   ggAGGTCTTGCATGATCTTCGGCATCTTCAAACCCTACCCGTCGCCGCTGATCAGCGGCCGTGGCATCGACCTGGACTTCCCGATGCTCGCCGGTTGCCTGGCCTTGCTGGGCCTGGGCCTGGTGATGATCACTTCGGCCTCCTCGGAAg                                              >  2:1050212/1‑150 (MQ=255)
                                                                                                    gAGGTCTTGCATGATCTTCGGCATCTTCAAACCCTACCCGTCGCCGCTGATCAGCGGCCGTGGCATCGAc                                                                                                                             >  2:1172996/1‑70 (MQ=255)
                                                                                                    gAGGTCTTGCATGATCTTCGGCATCTTCAAACCCTACCCGTCGCCGCTGATCAGCGGCCGTGGCATCGAc                                                                                                                             <  1:1172996/70‑1 (MQ=255)
                                                                                                    gAGGTCTTGCATGATCTTCGGCATCTTCAAACCCTACCCGTCGCCGCTGATCAGCGGCCGTGGCATCGACCTGGACTTCCCGATGCTCGCCGGTTGCCTGGCCTTGCTGGGCCTGGGCCTGGTGATGATCACTTCGGCCTCCTCGGAAGt                                             <  1:158549/150‑1 (MQ=255)
                                                                                                          ttGCATGATCTTCGGCATCTTCAAACCCTACCCGTCGCCGCTGATCAGCGGCCGTGGCATCGACCTGGACTTCCCGATGCTCGCCGGTTGCCTGGCCTTGCTGGGCCTGGGCCTGGTGATGATCACTTCGGCCTCCTCGGAAGTGGCTGc                                       <  1:406631/150‑1 (MQ=255)
                                                                                                           tGCATGATCTTCGGCATCTTCAAACCCTACCCGTCGCCGCTGATCAGCGGCCGTGGCATCGACCTGGACTTCCCGATGCTCGCCGGTTGCCTGGCCTTGCTGGGCCTGGGCCTGGTGATGATCACTTCGGc                                                         >  2:1014480/1‑131 (MQ=255)
                                                                                                           tGCATGATCTTCGGCATCTTCAAACCCTACCCGTCGCCGCTGATCAGCGGCCGTGGCATCGACCTGGACTTCCCGATGCTCGCCGGTTGCCTGGCCTTGCTGGGCCTGGGCCTGGTGATGATCACTTCGGc                                                         <  1:1014480/131‑1 (MQ=255)
                                                                                                             cATGATCTTCGTCATCTTCAAACCCTACCCGTCGCCGCTGAACAGCGGCCGTGCCAGCATTTCCGACTTCCCGATGCTCGCCGGTTGCCTGGCCTTGCTGGGCCTGGGCCTGGTGATGATCACTTCGGCCTCCTCGGAAGTGGCTGCGGt                                    >  2:1008883/1‑150 (MQ=255)
                                                                                                              aTGATCTTGGGCATCTTCAAACCCTACCCGTCGCCGCTGATCAGCGGCCGTGGCATCGACCTGGACTTCCCGATGCTCGCCGGTTGCCTGGCCTTGCTGGGCCTGGGCCTGGTGATGATCACTTCGGCCTCCTCGGAAGTGGCTGCGGTa                                   <  2:171099/150‑1 (MQ=255)
                                                                                                              aTGATCTTCGGCATCTTCAAACCCTACCCGTCGCCGCTGATCAGCGGCCGTGGCATCGACCTGGACTTCCCGATGCTCGCCGGTTGCCTGGCCTTGCTGGGCCTGGGCCTGGTGATGATCACTTCg                                                           >  2:805163/1‑126 (MQ=255)
                                                                                                              aTGATCTTCGGCATCTTCAAACCCTACCCGTCGCCGCTGATCAGCGGCCGTGGCATCGACCTGGACTTCCCGATGCTCGCCGGTTGCCTGGCCTTGCTGGGCCTGGGCCTGGTGATGATCACTTCg                                                           <  1:805163/126‑1 (MQ=255)
                                                                                                                gATCTTCGGCATCTTCAAACCCTACCCGTCGCCGCTGATCAGCGGCCGTGGCATCGACCTGGACTTCCCGATGCTCGCCGGTTGCCTGGCCTTGCTGGGCCTGGGCCTGGTGATGATCACTTCGGCCTCCTCGGAAGTGGCTGCGGTACa                                 >  1:477352/1‑150 (MQ=255)
                                                                                                                        gCATCTTCAAACCCTACCCGTCGCCGCTGATCAGCGGCCGTGGCATCGACCTGGACTTCCCGATGCTCGCCGGTTGCCTGGCCTTGCTGGGCCTGGGCCTGGTGATGATCACTTCGGCCTCCTCGGAAGTGGCTGCGGTACAGTCCGGCa                         >  2:1211196/1‑150 (MQ=255)
                                                                                                                          aTCTTCAAACCCTACCCGTCGCCGCTGATCAGCGGCCGTGGCATCGACCTGGACTTCCCGATGCTCGCCGGTTGCCTGGCCTTGCTGGGCCTGGGCCTGGTGATGATCACTTCGGCCTCCTCGGAAGTGGCTGCGGTACAGTCCGGCAAc                       >  1:837677/1‑150 (MQ=255)
                                                                                                                             ttCAAACCCTACCCGTCGCCGCTGATCAGCGGCCGTGGCATCGACCTGGACTTCCCGATGCTCGCCGGTTGCCTGGCCTTGCTGGGCCTGGGCCTGGTGATGATCACTTCGGCCTCCTCGGAAGTGGCTGCGGTACAGTCCGGCAACCCg                    >  1:308985/1‑150 (MQ=255)
                                                                                                                                  aCCCTACCCGTCGCCGCTGATCAGCGGCCGTGGCATCGACCTGGACTTCCCGATGCTCGCCGGTTGCCTGGCCTTGCTGGGCCTGTGTCTAGTGATGATCACTTCGGCCCCCTCGGAAGTGGCTGCGGTACAGTCCGGCAACCCGCTGTa               >  2:1127019/1‑150 (MQ=255)
                                                                                                                                  aCCCTACCCGTCGCCGCTGATCAGCGGCCGTGGCATCGACCTGGACTTCCCGATGCTCGCCGGTTGCCTGGCCTTGCTGGGCCTGGGCCTGGTGATGATCACTTCGGCCTCCTCGGAAGTGGCTGCGGTACAGTCCGGCAACCCGCTGTa               >  2:647003/1‑150 (MQ=255)
                                                                                                                                       aCCCGTCGCCGCTGATCAGCGGCCGTGGCATCGACCTGGa                                                                                                                        <  1:373116/40‑1 (MQ=255)
                                                                                                                                       aCCCGTCGCCGCTGATCAGCGGCCGTGGCATCGACCTGGa                                                                                                                        >  2:373116/1‑40 (MQ=255)
                                                                                                                                       aCCCGTCGCCGCTGATCAGCGGCCGTGGCATCGACCTGGACTTCCCGATGCTCGCCGGTTGCCTGGCCTTGCTGGGCCTGGGCCTGGTGATGATCACTTCGGCCTCCTCGGAAGTGGCTGCGGTGCAGTCCGGCAACCCGCTGTATcaca          <  1:815228/150‑1 (MQ=255)
                                                                                                                                       aCCCGTCGCCGCTGATCAGCGGCCGTGGCATCGACCTGGACTTCCCGATGCTCGCCGGTTGCCTGGCCTTGCTGGGCCTGGGCCTGGTGATGATCACTTCGGCCTCCTCGGAAGTGGCTGCGGTACAGTCCGGCAACCCGCTGTATcaca          >  1:566272/1‑150 (MQ=255)
                                                                                                                                         ccGTCGCCGCTGATCAGCGGCCGTGGCATCGACCTGGACTTCCCGATGCTCGCCGGTTGCCTGGCCTTGCTGGGCCTGGGCCTGGTGATGATCACTTCGGCCTCCTCGGAAGTGGCTGCGGTACAGTCCGGCAACCCGCTGTATCACATg        >  2:828045/1‑150 (MQ=255)
                                                                                                                                               ccgcTGATCAGCGGCCGTGGCATCGACCTGGACTTCCCGATGCTCGCCGGTTGCCTGGCCTTGCTGGGCCTGGGCCTGGTGATGATCACTTCGGCCTCCTCGGAAGTGGCTGCGGTACAGTCCGGCAACCCGCTGTATCACATGTTTCGc  <  1:828045/150‑1 (MQ=255)
                                                                                                                                                 gcTGATCAGCGGCCGTGGCATCGACCTGGACTTCCCGATGCTCGCCGGTTGCCTGGCCtt                                                                                          <  1:624227/60‑1 (MQ=255)
                                                                                                                                                 gcTGATCAGCGGCCGTGGCATCGACCTGGACTTCCCGATGCTCGCCGGTTGCCTGGCCtt                                                                                          >  2:624227/1‑60 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GCCCAGGCCGGCGATGCCGTGCTGCTGTCGCCAGCATGCGCCAGCCTCGACATGTTCAAGAACTTCGAGGAACGGGGACGCCTGTTCGCCCAGGCAGCGGGAGGTCTTGCATGATCTTCGGCATCTTCAAACCCTACCCGTCGCCGCTGATCAGCGGCCGTGGCATCGACCTGGACTTCCCGATGCTCGCCGGTTGCCTGGCCTTGCTGGGCCTGGGCCTGGTGATGATCACTTCGGCCTCCTCGGAAGTGGCTGCGGTACAGTCCGGCAACCCGCTGTATCACATGTTTCGC  >  NC_002947/1522025‑1522317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑