Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,563,870 A→C 9.0% intergenic (+94/+28) PP_1372 → / ← pitB hypothetical protein/phosphate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,563,8700AC 9.0% 160.7 / 7.6 63intergenic (+94/+28)PP_1372/pitBhypothetical protein/phosphate transporter
Reads supporting (aligned to +/- strand):  ref base A (29/28);  new base C (4/2);  total (33/30)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCGGCAGTTGGTGCGCGGGTTGATGGGGTCGTTGTTAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGACCCATGCCAGCAGGATGGTTTTCACAGTGCCGCCTTGCAGGCCACT  >  NC_002947/1563721‑1564014
                                                                                                                                                     |                                                                                                                                                
ggcggcAGGTGGTGGGCGGGTTGGTGGGGTCGTTGTTAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGa                                                                                                                                                  <  2:204955/150‑1 (MQ=255)
 gcggcAGTTGGTGCGCGGGTTGATGGGGTCGTTGTTAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTCTTGAGGACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAg                                                                                                                                                 >  1:80105/1‑150 (MQ=255)
              cgcgGGTTGATGGGGTCGTTGTTAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCttcattc                                                                                                                                    <  1:339210/150‑1 (MQ=255)
                 gggTTGATGGGGTCGTTGTTAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTcattcatt                                                                                                                                 >  1:1268840/1‑150 (MQ=255)
                   gTTGATGGGGTCGTTGTTAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGc                                                                                                                               >  1:743508/1‑150 (MQ=255)
                   gTTGATGGGGTCGTTGTTAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGc                                                                                                                               >  1:1269789/1‑150 (MQ=255)
                     tGATGGGGTCGTTTTTAGGGGGCAAGAAAAGTTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGAACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGTAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCgg                                                                                                                             <  1:621920/150‑1 (MQ=255)
                      gATGGGGTCGTTGTTAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGgt                                                                                                                            >  1:727183/1‑150 (MQ=255)
                               gttgttAGGGGGCAAGAAAAGGTAGTCTTCCCGCGCCGAGCCATACCCTTGGTAGCAGACGCCAGTGCCGGCACAGAACAAGCTGGCGTAGGCATAACGTCTATGAAAAAGGCGCCCGCGGCGGCCTTCATTCATCGAGCTCGCACTCAg                                                                                                                   >  2:178325/1‑150 (MQ=255)
                                 tgttAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGcc                                                                                                                 <  2:743508/150‑1 (MQ=255)
                                     aGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATg                                                                                                             >  2:1185356/1‑150 (MQ=255)
                                     aGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATg                                                                                                             >  2:1208995/1‑150 (MQ=255)
                                         ggCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCtt                                                                                                         >  2:1205773/1‑150 (MQ=255)
                                          gCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCTCGGGCGCCtt                                                                                                                                         <  1:753124/117‑1 (MQ=255)
                                          gCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCTCGGGCGCCtt                                                                                                                                         >  2:753124/1‑117 (MQ=255)
                                          gCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCttt                                                                                                        <  2:704717/150‑1 (MQ=255)
                                           cAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCAGTGTCACTCAGCCAATGGCt                                                                                                          >  2:53468/1‑147 (MQ=255)
                                           cAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCAGTGTCACTCAGCCAATGGCt                                                                                                          <  1:53468/147‑1 (MQ=255)
                                              gAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCTCGGGCGCCttttt                                                                                                                                      >  2:410286/1‑113 (MQ=255)
                                              gAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCTCGGGCGCCttttt                                                                                                                                      <  1:410286/116‑4 (MQ=255)
                                                    gTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACgccagc                                                                                              <  1:1205773/150‑1 (MQ=255)
                                                        tCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAgg                                                                                                                                                >  2:970146/1‑96 (MQ=255)
                                                        tCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAgg                                                                                                                                                <  1:970146/96‑1 (MQ=255)
                                                         cTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGaa                                                                                         <  1:604305/150‑1 (MQ=255)
                                                             cTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCCAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAgg                                                                                     >  2:905527/1‑150 (MQ=255)
                                                                      gccATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGgccgcc                                                                            <  1:513093/150‑1 (MQ=255)
                                                                        cATACCCGTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAg                                                                          <  2:80105/150‑1 (MQ=255)
                                                                         aTACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGaa                                                                                         <  1:404821/134‑1 (MQ=255)
                                                                         aTACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGaa                                                                                         >  2:404821/1‑134 (MQ=255)
                                                                         aTACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAgg                                                                         >  1:644257/1‑150 (MQ=255)
                                                                          gaCCCTTGCTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGc                                                                        <  1:178325/149‑1 (MQ=255)
                                                                             ccTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCa                                                                     >  2:190196/1‑150 (MQ=255)
                                                                             ccTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGAGTGGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCa                                                                     >  1:392004/1‑150 (MQ=255)
                                                                                      cacaAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGaggcag                                                            <  2:958454/150‑1 (MQ=255)
                                                                                          aGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGgcaggcag                                                        <  1:1185356/150‑1 (MQ=255)
                                                                                             ccGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGt                                                     <  1:886824/150‑1 (MQ=255)
                                                                                                cTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAg                                                  <  2:1268840/150‑1 (MQ=255)
                                                                                                    ggCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGAcc                                              >  2:16387/1‑150 (MQ=255)
                                                                                                     gCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACgccagc                                                                                              >  2:1097994/1‑101 (MQ=255)
                                                                                                     gCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACgccagc                                                                                              <  1:1097994/101‑1 (MQ=255)
                                                                                                     gCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGAccc                                             <  2:841358/150‑1 (MQ=255)
                                                                                                       aaaGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGACCCAt                                           >  1:1010245/1‑150 (MQ=255)
                                                                                                       aaaGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGACCCAt                                           <  1:388459/150‑1 (MQ=255)
                                                                                                         aGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAg                                                                          >  1:120704/1‑117 (MQ=255)
                                                                                                         aGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAg                                                                          <  2:120704/117‑1 (MQ=255)
                                                                                                           aaCAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGcaggcagg                                                       >  1:200813/1‑134 (MQ=255)
                                                                                                           aaCAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGcaggcagg                                                       <  2:200813/134‑1 (MQ=255)
                                                                                                           aaCAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGACCCATGCca                                       >  2:953556/1‑150 (MQ=255)
                                                                                                                      gTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGa                                                                                                     >  1:1084721/1‑77 (MQ=255)
                                                                                                                      gTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGa                                                                                                     <  2:1084721/77‑1 (MQ=255)
                                                                                                                                cGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGACCCATGCCAGCAGGATGGTTTTCACAGTgc                  >  2:1163827/1‑150 (MQ=255)
                                                                                                                                cGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGACCCATGCCAGCAGGATGGTTTTCACAGTgc                  <  1:1208995/150‑1 (MQ=255)
                                                                                                                                   cTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGACAGGACCCATGCCCGCAGGATGGTTTTCACAGTgccgc               >  1:290456/1‑150 (MQ=255)
                                                                                                                                       gAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGACCCATGCCAGCAGGATGGTTTTCACAGTGCCGCCTTg           >  1:1206192/1‑150 (MQ=255)
                                                                                                                                         aaaaGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGACCCATGCCAGCAGGATGGTTTTCACAGTGCCGCCtt            <  1:891731/147‑1 (MQ=255)
                                                                                                                                         aaaaGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGACCCATGCCAGCAGGATGGTTTTCACAGTGCCGCCtt            >  2:891731/1‑147 (MQ=255)
                                                                                                                                         aaaaGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGACCCATGCCAGCAGGATGGTTTTCACAGTGCCGCCTTGCa         <  2:644257/150‑1 (MQ=255)
                                                                                                                                          gaaGGCGCCCTCGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGACCCATGCCAGCAGGATGGTTTTCACAGTGCCGCCTTGCAg        >  1:937120/2‑150 (MQ=255)
                                                                                                                                           aaGGCGCCCGAGGGCGCCTTCATTCATTGGGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGACCCATGCCAGCAGGATGGTTTTCACAGTGCCGCCTTGCAgg       <  1:199231/150‑1 (MQ=255)
                                                                                                                                            aGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGACCCATGCCAGCAGGATGGTTTTCACAGTGCCGCCTTGCAGGc      <  1:953556/150‑1 (MQ=255)
                                                                                                                                            aGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGACCCATGCCAGCAGGATGGTTTTCACAGTGCCGCCTTGCAGGc      >  2:711229/1‑150 (MQ=255)
                                                                                                                                                gcCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGACCCATGCCAGCAGGATGGTTTTca                        <  2:1154982/128‑1 (MQ=255)
                                                                                                                                                gcCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGACCCATGCCAGCAGGATGGTTTTca                        >  1:1154982/1‑128 (MQ=255)
                                                                                                                                                gcCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGACCCATGCCAGCAGGATGGTTTTCACAGTGCCGCCTTGCAGGCCACt  >  1:722674/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
GGCGGCAGTTGGTGCGCGGGTTGATGGGGTCGTTGTTAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGACCCATGCCAGCAGGATGGTTTTCACAGTGCCGCCTTGCAGGCCACT  >  NC_002947/1563721‑1564014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑