| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,786,064 | T→A | 17.0% | intergenic (+38/‑148) | rpsB → / → tsf | 30S ribosomal protein S2/elongation factor Ts |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,786,064 | 0 | T | A | 17.0% | 109.5 / 18.4 | 47 | intergenic (+38/‑148) | rpsB/tsf | 30S ribosomal protein S2/elongation factor Ts |
| Reads supporting (aligned to +/- strand): ref base T (21/18); new base A (5/3); total (26/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.42e-01 | |||||||||||
TCGAGCTGTACATGACTTCGATGGCTGACGCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCAAGAGGATTTTGAAAT > NC_002947/1785917‑1786213 | tCGAGCTGTACATGACTTCGATGGCTGACGCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCttt > 1:118163/1‑150 (MQ=255) cTTCGATGGCTGACGCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCa > 1:276112/1‑150 (MQ=255) tGACGCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAGGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCGACTTGGCACGCAAGAAGCGGGCTTTGGCCCATTTTTACCACCTTGCAAtt > 2:928834/1‑149 (MQ=255) tGACGCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATc < 1:317156/150‑1 (MQ=255) gACGCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATcc < 1:995633/150‑1 (MQ=255) gCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCctgc > 1:40778/1‑150 (MQ=255) aTCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAg < 1:72845/150‑1 (MQ=255) gCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGc < 1:332032/150‑1 (MQ=255) caacaaCGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCg > 1:518701/1‑150 (MQ=255) aCCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCtgaaa > 2:735957/1‑150 (MQ=255) ccGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCtgaaat < 1:735957/150‑1 (MQ=255) gTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGTACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTCAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCATGCTGAAATGaaaa < 2:118163/150‑1 (MQ=255) gCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGTCACGCAAAAAGGGGGCTTTGCCCCCtt < 2:788789/81‑1 (MQ=255) gCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCtt > 1:788789/1‑81 (MQ=255) tGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGAt > 1:703285/1‑150 (MQ=255) tGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGATTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACCGCGGAt > 1:138106/1‑150 (MQ=255) aagCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCa < 2:703285/150‑1 (MQ=255) gCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGaa > 1:1117804/1‑150 (MQ=255) gCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGaa < 1:115612/150‑1 (MQ=255) ctgctgAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCt < 2:799166/49‑1 (MQ=255) ctgctgAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCt > 1:799166/1‑49 (MQ=255) aGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGa > 1:1031993/1‑150 (MQ=255) aGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGa > 1:392456/1‑150 (MQ=255) tAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCctgc < 2:1239352/69‑1 (MQ=255) tAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCctgc > 1:1239352/1‑69 (MQ=255) tAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGt < 2:392456/150‑1 (MQ=255) tAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGt < 2:1031993/150‑1 (MQ=255) acgcgcTAGCGTCTACTTGTCACGCAAAAGGGGGGCTTTGCGCGCTTTTTGCGACCTTGAAATCATGCGGTCAGCATCGACGCCGCATCATGGGCCTGCTGAAATGAAGACAGCGGGATTGCAGAAATGAACGCCCGTGATGGACGGGTg > 2:522067/3‑150 (MQ=255) aGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGt < 2:518701/150‑1 (MQ=255) aCTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGaaaaa > 1:705446/1‑150 (MQ=255) cTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCa > 2:236860/1‑108 (MQ=255) cTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCa < 1:236860/108‑1 (MQ=255) cTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAAc > 1:538632/1‑150 (MQ=255) cTTGGCACGCAAAAAGGGGGCTTTGCCCCCTCTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGAttt > 1:170034/1‑105 (MQ=255) cTTGGCACGCAAAAAGGGGGCTTTGCCCCCTCTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGAttt < 2:170034/105‑1 (MQ=255) tggCAAAAAGGGGGCAAAGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCa > 2:170813/3‑150 (MQ=255) aaaaaGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGccc > 2:1182066/1‑111 (MQ=255) aaaaaGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGccc < 1:1182066/111‑1 (MQ=255) aaaaaGGGGGCAAAGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCtgaaat > 2:1092121/1‑80 (MQ=38) aaaaaGGGGGCAAAGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCtgaaat < 1:1092121/80‑1 (MQ=38) aaaaaGGGGGCAAAGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCtgaaa < 2:1166751/79‑1 (MQ=37) aaaaaGGGGGCAAAGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCtgaaa > 1:1166751/1‑79 (MQ=37) aaaaaGGGGGCAAAGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGc > 2:804367/1‑89 (MQ=38) aaaaaGGGGGCAAAGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGc < 1:804367/89‑1 (MQ=38) aaaaaGGGGGCAAAGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCAagag > 1:1022921/1‑150 (MQ=255) aaaaGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGTCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGtt > 1:656860/1‑132 (MQ=255) aaaaGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGTCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGtt < 2:656860/132‑1 (MQ=255) ggggCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCAAGAGGAtttt > 1:423365/1‑150 (MQ=255) gggCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCAAGAGGATTTTg < 2:1186708/150‑1 (MQ=255) ggCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCACCATAGGCGACGCAGCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCAAGAGGATTTTGa > 1:1186708/1‑150 (MQ=255) tttGCCCCCTTTTTTCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCAAGAGGATTTTGAAAt > 2:1175389/1‑150 (MQ=255) | TCGAGCTGTACATGACTTCGATGGCTGACGCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCAAGAGGATTTTGAAAT > NC_002947/1785917‑1786213 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |